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Cadmium pollution bioremediation agent and its preparation method and application

A bioremediation agent and cadmium-polluted technology, applied in the field of agricultural microbiology, can solve the problems of safety standards, medium and light pollution levels, etc., and achieve the effects of reasonable types, reduced absorption, and strong comprehensive functions

Active Publication Date: 2016-03-16
HUNAN TAIGU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many reports on the application of microorganisms to remediate soil heavy metal pollution in China, and some scholars believe that it is difficult for bioremediation to meet the safety standards for medium and light pollution levels in a short period of time

Method used

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  • Cadmium pollution bioremediation agent and its preparation method and application
  • Cadmium pollution bioremediation agent and its preparation method and application
  • Cadmium pollution bioremediation agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Composition of cadmium pollution bioremediation agent

[0035] 15 parts of coreulin;

[0036] 15 servings of nutrients;

[0037] 4 parts of enzyme active substance;

[0038] 66 parts of bacterin carrier.

[0039] The composition of core bacteria

[0040] Rhodopseudomonas palustris 40.0×10 8 cfu / g;

[0041] Bacillus cereus 10.0×10 8 cfu / g;

[0042] Beer yeast 10.0×10 8 cfu / g;

[0043] Total effective bacteria content≥60.0×10 8 cfu / g.

[0044] The core bacteria of the above composition are prepared by the following steps:

[0045] Preparation of mixed spore powder:

[0046] ① Slant culture: The original strains of Rhodopseudomonas palustris, Bacillus cereus and Saccharomyces cerevisiae are respectively inoculated on the slant medium under aseptic conditions, and cultivated at 29±1℃ for 40h;

[0047] ② Shaker culture: Inoculate the strains cultivated in the above step ① into liquid culture medium under aseptic conditions, and culture them on a shaker at 140r / min for 36h under the ...

Embodiment 2

[0057] Example 2 Composition of cadmium pollution bioremediation agent

[0058] Core Bacterin 20 parts;

[0059] 20 servings of nutrients;

[0060] 5 parts of enzyme active substance;

[0061] 55 parts of bacterin carrier.

[0062] The composition of core bacteria

[0063] Rhodopseudomonas palustris 30.0×10 8 cfu / g;

[0064] Bacillus cereus 15.0×10 8 cfu / g;

[0065] Beer yeast 15.0×10 8 cfu / g;

[0066] Total effective bacteria content≥60.0×10 8 cfu / g.

[0067] Preparation:

[0068] ① Slope culture: The original strains of Rhodopseudomonas palustris, Bacillus cereus and Saccharomyces cerevisiae are respectively inoculated on the slant medium under aseptic conditions, and cultivated at 29±1℃ for 48h;

[0069] ② Shaker culture: Inoculate the strains cultivated in the above step ① into liquid culture medium under aseptic conditions, and culture them on a shaker at 160r / min for 48h under the conditions of pH7.0 and 30℃;

[0070] ③Fermentor culture: The bacteria cultured in the above step ② are respe...

Embodiment 3

[0073] Example 3 Composition of cadmium pollution bioremediation agent

[0074] Core Bacterin 10 parts;

[0075] 10 servings of nutrients;

[0076] 6 parts of enzyme activity material;

[0077] 74 copies of bacterin carrier.

[0078] The composition of core bacteria

[0079] Rhodopseudomonas palustris 50.0×10 8 cfu / g;

[0080] Bacillus cereus 5.0×10 8 cfu / g;

[0081] Brewer's yeast 5.0×10 8 cfu / g;

[0082] Total effective bacteria content≥60.0×10 8 cfu / g.

[0083] The medium used in each step of preparing the mixed spore powder in the above implementation is the same as the medium used in the corresponding steps in Example 1. The preparation method of the product is the same, and the nutrient components, enzyme active substances, and bacteria carrier are the same as in Example 1, except for the preparation The ratio is different.

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Abstract

The invention provides a cadmium pollution bioremediation agent which is composed of the following ingredients in parts by weight: 10-20 parts of core rhzomorph, 10-20 parts of a nutritional ingredient, 2-6 parts of an enzyme activity substance and 54-78 parts of an rhzomorph carrier. The invention further provides an application of the cadmium pollution bioremediation agent. The cadmium pollution bioremediation agent is reasonable in microorganism specie collocation and high in comprehensive function. Microorganisms in the core rhzomorph can be planted and grow in soil rapidly to form strong bacterial colonies, and heavy metal ions, namely, cadmium Cd<+1> can be directly absorbed to microbial cell walls or into cells to be cured and passivated. Besides, bacillus cereus and beer yeast in the composition have high absorption and passivation function on heavy metal ions, namely, cadmium Cd<+1>, chromium Cr<+1> and lead Pb<+1>, due to the high comprehensive function, the mobility of the heavy metal ions, namely, cadmium Cd<+1>, is reduced, and the purpose of reducing absorption of crops on the heavy metal ions is achieved.

Description

Technical field [0001] The invention belongs to the application field of agricultural microorganisms, and specifically relates to a bioremediation agent containing active bacteria and a preparation method and application thereof. Background technique [0002] With the development of industrialization and agricultural intensive economy, the heavy metal pollution of cultivated land in my country is becoming more and more serious. In the early 1990s, the area of ​​cultivated land polluted by cadmium in my country reached 1.3×104 / hm 2 , Involving 25 regions in 11 provinces and cities. According to the special survey of heavy metal pollution in Hunan Province and the results of agricultural environmental quality monitoring in recent years, the contaminated cultivated land in Hunan Province already accounts for 23.7 of the total cultivated land in the province. There are also about 27% of farmland irrigation water and about 25% of farmland. The atmosphere has been polluted to varying ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/42C09K101/00
Inventor 唐八生曹典军郭帅蔡浩侯亚卓周艳周利群
Owner HUNAN TAIGU BIOTECH
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