Method for promoting growth of plants on manganese tailing residues

A technology of tailings slag and plants, applied in the field of environmental biology

Inactive Publication Date: 2012-03-28
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many shortcomings such as high cost and secondary pollution caused by engineering restoration and chemical restoration. Bioremediation technology has become the preferred method for repairing tailings pollution due to its advantages of simple process, low cost, and environmental friendliness.
At present, there are no plants on the surface of many tailings ponds in my country, or there are plants but very few in number, and their growth is slow, and they cannot form vegetation covering the entire tailings wasteland, and the tailings ponds cannot be repaired.
Some plants with large biomass and tolerance to enrichment of heavy metals cannot grow in tailings ponds, so finding a way to promote the growth of plants in tailings ponds is an important part of tailings pond governance
[0003]Research shows that the lack of vegetation in tailing ponds is due to high content of heavy metals, poor water retention, lack of nutrients for plant growth, etc.
Our research has found that there are bacteria in the tailings ponds without growing vegetation, mainly bacteria, molds, actinomycetes, etc., but the number is very small, the growth is slow, and most of them are in a dormant state, which cannot make the tailings ponds suitable for plant growth. environmental changes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of indigenous bacteria culture solution: its composition is 5g / L of soluble starch, 1g / L of shea butter, 3g / L of peptone, 0.2 g / L of dipotassium hydrogen phosphate, 5g / L of sodium chloride, Sucrose 3g / L, magnesium sulfate 0.2 g / L, ferrous sulfate 0.2 g / L, potassium nitrate 0.5g / L, urea 10g / L, superphosphate 5g / L, adjusted with hydrochloric acid or sodium hydroxide solution The pH is 6.5-7.5.

[0025] (2) Obtaining the concentrated solution of indigenous bacteria: collect 10g of tailings slag to be repaired, add it to a conical flask filled with 100ml of sterile water, place it on a shaker, and shake it at a speed of 100 rpm for 10 minutes. Then take 20ml of the supernatant and add it into 980ml of native bacteria culture solution. Divide the culture solution inoculated with indigenous bacteria, place it in a shaker at 30°C and culture it with shaking at 200 revolutions per minute, and cultivate it for 3-5 days. Stop the culture when the absorbance of th...

Embodiment 2

[0029] (1) Take the manganese tailings slag from the tailings pond of Xiangtan Manganese Mine, which has no plant growth, and then air-dry it naturally for later use; prepare the water-retaining gel fungus bed and the water-retaining gel fungus bed inoculated with native bacteria according to the method described in Example 1 ,spare.

[0030] (2) Use a flower pot with a diameter of 20cm and a height of 20cm, and pack three kinds of cultures, which are 100% manganese tailings slag (control), water-retaining gel bacteria bed with a quality of 1:6 without inoculation and Manganese tailings slag mixture (treatment 1), water-retaining gel fungus bed with a quality of 1:6 inoculum and manganese tailings slag mixture (treatment 2), the volume of the culture is about 4 / 5 of the volume of the flowerpot, after installation, Water 150ml per pot, put a watertight plate under the flower pot, and place it in the natural environment of Hunan University of Science and Technology.

[0031] (3...

Embodiment 3

[0036] (1) According to the method described in Example 1, a water-retaining gel bed inoculated with indigenous bacteria was prepared and set aside.

[0037] (2) Build a planting ditch on the tailings pond of a manganese mine in Hunan where no plants grow. The specifications of the planting ditch are: length 15cm, width 15cm, depth 10cm, and the distance between the edges of each planting ditch is 30cm.

[0038] (3) Mix the water-retaining bacteria bed gel and slag inoculated with indigenous bacteria at a mass ratio of 1:4-8, and place them in the planting ditch.

[0039] (4) In April, select Pokeweed in the 3-4 leaf stage ( Phytolacca americana) seedlings were transplanted into the planting ditch, and 2 seedlings were planted in each planting ditch, covered with 3-4 cm of tailings slag, and watered 3 times.

[0040] (5) Careful management and reasonable fertilization. According to statistics in June, the survival rate of pokeweed seedlings was 96%.

[0041] (6) In Oc...

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PUM

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Abstract

The invention relates to a method for promoting growth of plants on manganese tailing residues. Used indigenous bacteria come from the tailing residues on which plants do not grow. Used plants are Phytolacca americana. An inoculated water-retaining gel bacteria bed and the tailing residues are evenly mixed according to a mass ratio being 1: (4-8) to cultivate Phytolacca americana seedlings. The plants can be promoted to grow and breed on the manganese tailing residues to form vegetation. The water-retaining gel bacteria bed contains 5 parts of white mica, 5 parts of montmorillonite, 7 parts of agar and 83 parts of indigenous bacteria culture solution. The indigenous bacteria culture solution contains soluble starch with concentration being 5g / L, beef tallow cream with concentration being 1g / L, peptone with concentration being 3g / L, dipotassium hydrogen phosphate with concentration being 0.2g / L, sodium chloride with concentration being 5g / L, cane sugar with concentration being 3g / L, magnesium sulfate with concentration being 0.2g / L, ferrous sulfate with concentration being 0.2g / L, potassium nitrate with concentration being 0.5g / L, urea with concentration being 10g / L and calcium superphosphate with concentration being 5g / L. The method has the advantages that the operation is simple, the cost is low, the efficiency of resisting and controlling the migration and the diffusion of manganese and other harmful metal in manganese tailing residues, the environment can be beautified and the like.

Description

technical field [0001] The invention relates to the field of environmental biotechnology, in particular to a method for promoting the growth and reproduction of plants in the tailings slag by using the high water-retaining gel bacteria bed and the native bacteria of the tailings pond, increasing the biomass of the plants and improving the efficiency of phytoremediation. Background technique [0002] The mining and smelting of mines have made great contributions to the development of human society, but left waste tailings that are harmful to humans, animals and plants. The long-term stacking of tailings not only takes up a lot of land, but also has a serious impact on the surrounding ecosystem. Due to the lack of nutrients and high content of heavy metals in the tailings after smelting, there is almost no vegetation in the tailings pool in the natural state. Due to the small particle size of mine tailings, tailing sandstorms are likely to occur when the wind blows, and tailin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01B79/02C12N1/00
Inventor 严明理冯涛向言词曾荣今刘丽莉李会东朱佳文
Owner HUNAN UNIV OF SCI & TECH
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