Method for promoting growth of plants on manganese tailing residues
A technology of tailings slag and plants, applied in the field of environmental biology
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Embodiment 1
[0024] (1) Preparation of indigenous bacteria culture solution: its composition is 5g / L of soluble starch, 1g / L of shea butter, 3g / L of peptone, 0.2 g / L of dipotassium hydrogen phosphate, 5g / L of sodium chloride, Sucrose 3g / L, magnesium sulfate 0.2 g / L, ferrous sulfate 0.2 g / L, potassium nitrate 0.5g / L, urea 10g / L, superphosphate 5g / L, adjusted with hydrochloric acid or sodium hydroxide solution The pH is 6.5-7.5.
[0025] (2) Obtaining the concentrated solution of indigenous bacteria: collect 10g of tailings slag to be repaired, add it to a conical flask filled with 100ml of sterile water, place it on a shaker, and shake it at a speed of 100 rpm for 10 minutes. Then take 20ml of the supernatant and add it into 980ml of native bacteria culture solution. Divide the culture solution inoculated with indigenous bacteria, place it in a shaker at 30°C and culture it with shaking at 200 revolutions per minute, and cultivate it for 3-5 days. Stop the culture when the absorbance of th...
Embodiment 2
[0029] (1) Take the manganese tailings slag from the tailings pond of Xiangtan Manganese Mine, which has no plant growth, and then air-dry it naturally for later use; prepare the water-retaining gel fungus bed and the water-retaining gel fungus bed inoculated with native bacteria according to the method described in Example 1 ,spare.
[0030] (2) Use a flower pot with a diameter of 20cm and a height of 20cm, and pack three kinds of cultures, which are 100% manganese tailings slag (control), water-retaining gel bacteria bed with a quality of 1:6 without inoculation and Manganese tailings slag mixture (treatment 1), water-retaining gel fungus bed with a quality of 1:6 inoculum and manganese tailings slag mixture (treatment 2), the volume of the culture is about 4 / 5 of the volume of the flowerpot, after installation, Water 150ml per pot, put a watertight plate under the flower pot, and place it in the natural environment of Hunan University of Science and Technology.
[0031] (3...
Embodiment 3
[0036] (1) According to the method described in Example 1, a water-retaining gel bed inoculated with indigenous bacteria was prepared and set aside.
[0037] (2) Build a planting ditch on the tailings pond of a manganese mine in Hunan where no plants grow. The specifications of the planting ditch are: length 15cm, width 15cm, depth 10cm, and the distance between the edges of each planting ditch is 30cm.
[0038] (3) Mix the water-retaining bacteria bed gel and slag inoculated with indigenous bacteria at a mass ratio of 1:4-8, and place them in the planting ditch.
[0039] (4) In April, select Pokeweed in the 3-4 leaf stage ( Phytolacca americana) seedlings were transplanted into the planting ditch, and 2 seedlings were planted in each planting ditch, covered with 3-4 cm of tailings slag, and watered 3 times.
[0040] (5) Careful management and reasonable fertilization. According to statistics in June, the survival rate of pokeweed seedlings was 96%.
[0041] (6) In Oc...
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