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Application of chitosan derivative in bacterial wilt prevention and treatment

A technology for chitosan derivatives and bacterial wilt disease, which is applied in the application field of chitosan derivatives in the prevention and control of bacterial wilt disease, can solve problems such as large environmental impact, and achieve the effect of no pollution

Inactive Publication Date: 2015-12-30
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the effect of chemical control is very good, it has a relatively large impact on the environment

Method used

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  • Application of chitosan derivative in bacterial wilt prevention and treatment
  • Application of chitosan derivative in bacterial wilt prevention and treatment
  • Application of chitosan derivative in bacterial wilt prevention and treatment

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] The preparation method of chitosan derivative solution comprises the following steps:

[0024] Weigh a small amount of chitosan derivatives, dissolve them in dilute acid solution, and prepare mother liquor. The dosage and concentration can be diluted as required.

[0025] The antibacterial effect of chitosan derivatives was determined by spectrophotometry.

[0026] Spectrophotometric method: use a microplate reader to measure the absorbance of the bacterial solution added with different concentrations of chitosan derivatives, and accurately determine its inhibitory rate and half-inhibitory concentration on R. solanacearum. The formula for calculating the antibacterial rate is formula (II):

[0027]

[0028] Among them, the T0 sample and the TF sample are the absorbance of the bacterial solution adding chitosan derivatives before and after cultivation; the T0 blank and the TF blank are the absorbance of only adding the culture medium before and after cultivation; the...

Embodiment 1

[0030] In this example, spectrophotometry is used to detect the inhibitory effect of chitosan derivatives in the lowest concentration range on the pathogenic bacteria of mulberry wilt.

[0031] Mulberry wilt pathogen RS-5 was cultured with shaking at 28° C. for 24 hours in TM liquid medium (10 g of peptone, 5 g of glucose, 1 g of casein, and 1 L of distilled water) to obtain a bacterial suspension. Dissolve chitosan derivatives in dilute acid, prepare a 20g / L solution, and filter with a 0.22μm microporous membrane to remove the influence of bacteria. Add 5mLTM liquid medium to each test tube to set different concentrations of chitosan derivatives (CTS-g-CA, CTS-g-FA, CTS-g-CIA, CTS-g-COA and CTS-g-CHA) , with a structure such as figure 1 shown. Mulberry wilt pathogen RS-5 was inserted into the test tube, and three replicates were done, and cultured overnight. Finally, 200 μL of the bacterial suspension in the test tube was added to a 96-well plate, read at 600 nm with a mic...

Embodiment 2

[0034] In this example, spectrophotometry was used to detect the inhibitory effect of chitosan derivatives in the highest concentration range on the pathogenic bacteria of mulberry wilt.

[0035]The pathogen RS-5 of mulberry wilt was cultured with shaking in TM liquid medium at 28°C for 24 hours to obtain a bacterial suspension. Dissolve chitosan and its derivatives in dilute acid, prepare a 20g / L solution, and filter with a 0.22μm microporous membrane to remove the influence of bacteria. Add 5mLTM liquid medium to each test tube to set different concentrations of chitosan derivatives (CTS-g-CA, CTS-g-FA, CTS-g-CIA, CTS-g-COA and CTS-g-CHA) , with a structure such as figure 1 shown. Mulberry wilt pathogen RS-5 was inserted into the test tube, and three replicates were done, and cultured overnight. Finally, 200 μL of the bacterial suspension in the test tube was added to a 96-well plate, read at 600 nm with a microplate reader, and the bacteriostatic rate was calculated acco...

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Abstract

The invention relates to application of a chitosan derivative in bacterial wilt prevention and treatment. The structure of the chitosan derivative is shown in the formula (I), wherein R represents H or caffeic acid, or ferulic acid, or cinnamic acid, or p-coumaric acid or chlorogenic acid with a decarboxylated hydroxyl group. The medial lethal concentration of the chitosan derivative used for restraining bacterial wilt pathogenic bacteria is provided, the low-concentration chitosan derivative can be used for effectively restraining bacterial wilt bacteria, an effective method for preventing and treating bacterial wilt is provided, and the environment is not polluted.

Description

technical field [0001] The invention belongs to the field of microorganism prevention and control, and in particular relates to the application of chitosan derivatives in the prevention and treatment of bacterial wilt. Background technique [0002] Ralstonia solanacearum (Ralstonia solanacearum) is one of the most destructive plant bacterial diseases. Its pathogenic bacteria is Ralstonia solanacearum. The pathogen of ephedra bacterial wilt GIM1.76, the pathogen of tomato bacterial wilt GIM1000, the pathogen of ginger bacterial wilt GIM1.71 and the pathogen of mulberry bacterial wilt RS-5 are mainly distributed in tropical, subtropical and temperate regions, and endanger more than 50 families and three species. More than a hundred kinds of plants have caused a large area of ​​plant diseases, which has restricted the sustainable development of the economy to a certain extent. [0003] Under natural conditions, R. solanaceae can invade from wounds in plant roots or stems, or d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P1/00C12P19/26
Inventor 吴福安杨采风周雨吴滔许晏盛晟王俊
Owner JIANGSU UNIV OF SCI & TECH
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