Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Engineering bacterium for microbial synthesis of piceatannol by taking glucose as substrate as well as construction and application of engineering bacterium

A technology for microbial synthesis and piceatannol, applied in the biological field, can solve the problems of high cost, low yield, easy to be oxidized and the like, and achieve the effect of increasing the content

Pending Publication Date: 2022-04-26
河北维达康生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural piceatanol is extremely difficult to obtain, and only 0.3g of piceatanol can be extracted from 1kg of dry rhizomes of Phaphadont, and the chemical properties of piceatanol are unstable and easily oxidized, making the naturally extracted piceatanol Alcohol is extremely expensive
Because picatanol is easily oxidized and the cis-trans configuration is difficult to control, it is difficult to prepare
At present, there are two synthetic methods of picatanol: chemical synthesis and biosynthesis, and the chemical synthesis has the disadvantages of high cost, harsh reaction conditions, complicated operation, and low yield.
The biosynthesis of picetanol belongs to the green synthesis process, which has the characteristics of less waste and easy disposal, and has broad application prospects. However, the yield of biosynthesis of picatanol in the prior art is low, which cannot meet the requirements of industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Engineering bacterium for microbial synthesis of piceatannol by taking glucose as substrate as well as construction and application of engineering bacterium
  • Engineering bacterium for microbial synthesis of piceatannol by taking glucose as substrate as well as construction and application of engineering bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 produces the construction of picetanol engineering strain

[0039] 1. pINA1312-YIARO4 K211L -YIARO7 G139S Plasmid construction

[0040] (1) Artificially synthesized YIARO4 K211L Gene as template, using primer YIARO4 K211L -YIARO7 G139S -F1 and YIARO4 K211L -YIARO7 G139 -R1 is amplified by PCR, and the amplified product is recovered to obtain YIARO4 K211L The target fragment, the primer YIARO4 K211L -YIARO7 G139S -F1 sequence 5'-CCCCACGTGATGCCGCCGAAAGTGGTGAT-3', the primer YIARO4 K211L -YIARO7 G139S - R1 sequence 5'-CGGGGTACCTTTTCTGCGCGCGCATGCTAT-3';

[0041] (2) Using artificially synthesized YIARO7 G139S Gene as template, using primer YIARO4 K211L -YIARO7 G139S -F2 and YIARO4 K211L -YIARO7 G139S -R2 is amplified by PCR, and the amplified product is recovered to obtain YIARO7 G139S The target fragment, the primer YIARO4 K211L -YIARO7 G139S -F2 sequence 5'-CCCCACGTGATGGATTTTACCAAAGCGGATAC-3', the primer YIARO4 K211L -YIARO7 G139S - R2 se...

Embodiment 2

[0062] Embodiment 2 Yarrowia lipolytica engineering bacteria fed-batch fermentation

[0063] 1. Streak the engineering bacteria II on the YNB solid medium and cultivate until a single colony grows, pick a single colony, inoculate it into the YNB liquid medium, and cultivate it for 15 hours to obtain the seed liquid. The YNB medium: 20g / L Glucose is the carbon source, 6.7g / L amino acid-free yeast nitrogen source; the culture condition of the seed liquid is 30°C, 225rpm, the OD of the seed liquid 600 = 10;

[0064] 2. Insert 2% of the seed liquid into 2L fermentation medium to carry out fermentation culture; the components of the fermentation medium are: substrate glucose 8%, (NH 4 ) 2 SO 4 0.5%, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O 0.05%, trace metal solution 0.2%, vitamin solution 0.1%, defoamer 204 0.05%; the fermentation conditions are 30°C, 225rpm, and the initial pH is 6.0; in the fermentation stage, 20g / L glucose is added every 24 hours ; The pH was adjusted to 6.0 by...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an engineering bacterium for microbial synthesis of piceatannol by taking glucose as a substrate as well as construction and application of the engineering bacterium. According to the invention, L-tyrosine ammonolysis enzyme, p-coumaroyl-coenzyme A ligase, resveratrol synthetase, DAHP synthetase, chorismate mutase and hydroxylase complex genes are over-expressed in the engineering strain, so that the de novo synthesis strategy of piceatannol is realized, and the content of the product piceatannol is remarkably increased; and a simple and effective implementation method is provided for safe biotransformation. The yield of the engineering bacterium for synthesizing piceatannol by using the glucose as the substrate microorganism disclosed by the invention can reach 7-8g / L.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the construction and application of an engineering bacterium for biosynthesizing piceatanol with glucose as a substrate. Background technique [0002] Picetanol (3,3',4,5'-tetrahydroxytrans-stilbene) is a biologically active molecule, the 3'-hydroxylation product of resveratrol, found in grape, knotweed and passion fruit Exist in many kinds of natural plants. It is a very strong antioxidant, which shows higher antioxidant activity than resveratrol in the process of free radical scavenging; besides, picatanol can also inhibit leukemia, lymphoma and melanoma, etc. Proliferation of a variety of tumor cells; studies have confirmed that picatanol has various positive effects of improving immune regulation, anti-inflammation, antibacterial, inhibiting skin melanin production and alleviating arterial vasodilation. [0003] The many beneficial effects of picatanol on the human ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12N15/81C12P7/22C12R1/645
CPCC12Y403/01023C12Y602/01012C12Y203/01095C12N9/0004C12Y504/99005C12Y205/01054C12N9/1029C12N9/1085C12N9/88C12N9/93C12N15/815C12P7/22
Inventor 赵云现杨志彬胡江林展全乐朱志通赵凯
Owner 河北维达康生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com