The invention provides an engineering bacterium suitable for salicin, which comprises a host and a recombinant plasmid vector, and the recombinant plasmid vector is connected with a gene for expressing and coding glucosyltransferase. The recombinant plasmid vector is also connected with a gene for coding carboxylic acid reductase and phosphoryl transferase, which is co-expressed with the gene for coding glucosyltransferase. The recombinant plasmid vector is also connected with genes for over-expressing and coding shikimic acid kinase, pyruvate kinase, transketolase, 3-deoxy-7-phosphoenanthate synthase, iso-chorismate pyruvate lyase and isochorismate synthase; and the host is also knocked out of genes for coding pykA and pykF, so that the yield of salicin is increased. The invention also provides a construction method and an application of the engineering bacterium for synthesizing salicin. By utilizing the engineering bacteria for synthesizing the salicin, the salicin is produced by using glucose and/or glycerol and other carbon sources, so that efficient biosynthesis of the salicin is realized.