12 results about "Chorismic acid" patented technology

The embodiment of the invention provides a recombinant pichia pastoris strain as well as a construction method and application thereof. The construction method comprises the following steps: by taking a pichia pastoris strain as a host strain, constructing a dual-synthesis path of phenylalanine ammonialyase and tyrosine ammonialyase in the host strain to obtain a first engineering strain; removing chorismic acid feedback inhibition of the first engineering strain based on metabolic engineering to obtain a second engineering strain; optimizing an aromatic amino acid synthesis route of the second engineering strain to obtain a third engineering strain; knocking out a branch metabolic pathway of the third engineering strain to obtain a fourth engineering strain; increasing precursor supply of the fourth engineering strain to obtain a recombinant pichia pastoris strain; according to the construction method provided by the embodiment of the invention, the synthesis path of p-coumaric acid can be systematically optimized, the recombinant pichia pastoris strain for efficiently producing p-coumaric acid by taking methanol as a carbon source is constructed, and compared with a host strain, the recombinant pichia pastoris strain has the advantage that the yield of p-coumaric acid is remarkably increased.

The invention discloses a yarrowia lipolytica engineering strain with high yield of resveratrol as well as construction and application of the yarrowia lipolytica engineering strain, and belongs to the technical field of synthetic biology and metabolic engineering. The invention provides a yarrowia lipolytica engineering strain. A 4-coumaric acid-CoA ligase gene, a multi-copy resveratrol synthase gene, a transketolase 1 gene, a fusion gene of a histidine phosphate aminotransferase gene and a tyrosine ammonia lyase gene, a glycolytic pathway hexokinase gene, a 6-phosphofructokinase-1 gene and a phosphoglycerate kinase gene are integrated, so that the recombinant protein is obtained. According to the present invention, by using glucose as the substrate, the high yield of resveratrol can be achieved by using glucose as the substrate, the resveratrol yield during shake-flask culture fermentation can achieve 3.157 g / L, the resveratrol yield can be increased to 30.7 g / L during 5L-scale fed-batch fermentation, and the resveratrol yield can be increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L; the maximum value is reported by the yarrowia lipolytica system at present.

Aspects of the disclosure relate to methods and compositions for diagnosing and / or treating subjects having one or more symptoms of Autism Spectrum Disorder (ASD) or subjects at risk of developing ASD. In some embodiments, methods comprise determining and / or modulating levels of chorismate and / or a molecule within a chorismate metabolic pathway in a subject. In some embodiments, methods comprise determining and / or modulating levels of a microbial feature (e.g., a Sarcina bacterium) in a subject. In some embodiments, methods comprise determining and / or modulating levels of proton flux in a gastrointestinal tract in a subject.

The application provides a recombinant Pichia pastoris strain and a construction method and application thereof. The construction method comprises the following steps: taking a Pichia pastoris strain as a host strain, constructing a double synthesis pathway of phenylalanine ammonia lyase and tyrosine ammonia lyase in the host strain to obtain a first engineering strain; relieving the branch acid feedback inhibition of the first engineering strain based on metabolic engineering to obtain a second engineering strain; optimizing the aromatic amino acid synthesis pathway of the second engineering strain to obtain a third engineering strain; knocking out the branch metabolic pathway of the third engineering strain to obtain a fourth engineering strain; increasing the precursor supply of the fourth engineering strain to obtain the recombinant Pichia pastoris strain. The construction method can systematically optimize the synthesis path of p-coumaric acid, and the recombinant Pichia pastoris strain for efficiently producing p-coumaric acid with methanol as a carbon source is constructed. Compared with the host strain, the yield of p-coumaric acid of the recombinant Pichia pastoris strain is significantly improved.

This invention discloses a method for the efficient synthesis of gastrodin using recombinant Corynebacterium glutamicum, belonging to the fields of genetic engineering, fermentation engineering, and synthetic biology. This invention uses Corynebacterium glutamicum as the host bacterium and knocks out… pobA The gene was expressed, and the engineered strain WN01 / pB6 was constructed using the expression plasmid pEC-XK99E to express cladoid-pyruvate lyase, carboxylic acid reductase, and glycosyltransferase, achieving heterologous synthesis of gastrodin. Further overexpression was then performed. aroG Genes, overexpression of protein-coding genes that activate carboxylic acid reductase sfp The engineered strain WN05 / pB6 was able to synthesize 7.37 g / L of gastrodin at the shake flask level, and the gastrodin yield reached 17.77 g / L at 72 h of batch fermentation in a 5-L fermenter, with a yield of 0.247 g / L / h. This provides a brand-new biomanufacturing platform for the industrial production of gastrodin.

The invention provides a genetic engineering strain, a construction method of the genetic engineering strain and application of the genetic engineering strain in biosynthesis of 5-hydroxytryptophan. According to the engineering bacterium, four key modules required by 5-HTP synthesis are sequentially introduced into a host genome in a genome integration mode through a CRISPR technology, and the four key modules comprise an aroGfbr-ppsA-tktA module for enhancing chorismate precursor supply, a trpEfbr-trpG module for catalyzing o-aminobenzoic acid generation, a salABCD gene cluster responsible for hydroxylating o-aminobenzoic acid into 5-hydroxy o-aminobenzoic acid, and a salABCD gene cluster responsible for hydroxylating 5-hydroxy o-aminobenzoic acid into 5-hydroxy o-aminobenzoic acid. And a trpDCBA module for converting the intermediate into the final product 5-HTP. The modules are respectively integrated into a plurality of non-essential gene regions, so that stable genetic expression is realized, and a complete metabolic pathway can be operated without plasmids or inducers. The strain can continuously accumulate 5-hydroxytryptophan (5-HTP) under a glucose fermentation condition, and has the advantages of simplicity and convenience in construction, high hereditary stability, low production cost, suitability for industrial-scale fermentation and the like.

Disclosed herein are compositions (e.g., pharmaceutical compositions, nutraceutical compositions) and methods useful for, inter alia, modulating levels of certain chorismate metabolites in a subject (e.g., in the gut of a subject). Modulation of levels of certain chorismate metabolites in a subject (e.g., levels of the chorismate metabolites in the gut) may have beneficial effects, prophylactic, and / or therapeutic effects for the subject, for example, in enhancing cognitive function or treating one or more signs or symptoms of a neurological disorder.

The present invention provides a yeast genetic engineering bacterium for producing resveratrol and a construction method and application thereof. The engineering bacterium overexpresses resveratrol synthase on the chromosome of Saccharomyces cerevisiae BY4742. STS , p-Coumaryl-CoA ligase 4CL , phenylalanine / tyrosine ammonia lyase TAL , cinnamate-4-hydroxylase C4H , cytochrome P450 enzyme reductase CPR1 , DAHP synthase mutant ARO4 , chorismate mutase mutant ARO7 Chorismate synthase ARO2 shikimate kinase AroL , acetyl-CoA synthetase ACS and malonyl-CoA synthetase ACC1 , and knock out bypass genes CIT2, LPP1, DPP1, and PDC6 at the same time, to achieve biosynthesis of resveratrol using glucose as a substrate. The present invention utilizes phenylalanine / tyrosine ammonia lyase to react with phenylalanine and tyrosine as substrates to generate key precursors for resveratrol synthesis, strengthens the precursors required for resveratrol synthesis, and greatly increases the yield of the product resveratrol.

Provided are: a transformant capable of improving the productivity of 3-HBA; and a method for producing 3-HBA using said transformant. In one aspect, the present disclosure pertains to a transformant for producing 3-hydroxybenzoic acid (3-HBA), the transformant being obtained by expressibly introducing, into a microbial host having chorismic acid-producing ability, a gene (A) encoding an enzyme (3-HBA synthase) having activity for producing 3-HBA from chorismic acid, wherein the microbial host is Corynebacterium glutamicum. In another aspect, the present disclosure pertains to a method for producing 3-HBA, the method comprising a step for culturing this transformant to produce 3-HBA.

The application discloses a kind of recombinant escherichia coli and its construction method and application, the recombinant escherichia coli can be used for the production of L-phenylalanine.The application utilizes multiple rounds of mutagenesis screening to have high resistance to L-phenylalanine structural analogues, and has tyrosine auxotrophic mutant strain, and is regulated in the form of temperature-sensitive recombinant plasmid to code 3-deoxy-7-phosphoheptanoate synthase and branch acid mutase gene expression in L-phenylalanine synthesis pathway.The recombinant escherichia coli constructed in the application, in 5L fermenter, according to the cell growth stage adopts different temperature control and feeding mode, fermentation time is 56 h, under the condition that L-phenylalanine production is 62 g / L, sugar acid conversion rate is 25.2%, has the prospect and value of industrialization application.

The application discloses a high-yield resveratrol Yarrowia lipolytica engineering strain and construction and application thereof, and belongs to the technical field of synthetic biology and metabolic engineering. The Yarrowia lipolytica engineering strain provided by the application can realize high-yield resveratrol by using glucose as a substrate, and the yield of resveratrol reaches 3.157 g / L in shake flask fermentation, and the yield of resveratrol is increased to 30.7 g / L in 5L scale batch feeding fermentation, which is the highest value reported at present.

The invention relates to the technical field of microbial fermentation engineering, and discloses a method for directly producing tyrosol by using a recombinant Escherichia coli and recombinant Saccharomyces cerevisiae double-bacterium co-culture system and using low-cost glucose or other sugar sources as substrates, precursors do not need to be added, the preparation method is simple, and the cost is greatly reduced. The recombinant Escherichia coli is obtained by the following steps: carrying out genetic engineering modification, over-expressing glucose-6-phosphate dehydrogenase G6PDH to regulate and control a glucose utilization pathway, over-expressing tyrosine synthase tyrA and knocking out chorismate mutase pheA; the saccharomyces cerevisiae is obtained by introducing an exogenous way and overexpressing tyrosine decarboxylase AAS and phenylacetaldehyde reductase PAR, so that the tyrosol is directly and efficiently synthesized from a sugar source. The De novo synthesis yield of the tyrosol by adopting the double-bacterium co-culture system reaches 15.67 g / L, so that the De novo synthesis yield of the tyrosol is greatly improved, and a new route is provided for producing the tyrosol by efficient fermentation of microorganisms.