5 results about "Chorismic acid" patented technology

This invention discloses a method for the efficient synthesis of gastrodin using recombinant Corynebacterium glutamicum, belonging to the fields of genetic engineering, fermentation engineering, and synthetic biology. This invention uses Corynebacterium glutamicum as the host bacterium and knocks out… pobA The gene was expressed, and the engineered strain WN01 / pB6 was constructed using the expression plasmid pEC-XK99E to express cladoid-pyruvate lyase, carboxylic acid reductase, and glycosyltransferase, achieving heterologous synthesis of gastrodin. Further overexpression was then performed. aroG Genes, overexpression of protein-coding genes that activate carboxylic acid reductase sfp The engineered strain WN05 / pB6 was able to synthesize 7.37 g / L of gastrodin at the shake flask level, and the gastrodin yield reached 17.77 g / L at 72 h of batch fermentation in a 5-L fermenter, with a yield of 0.247 g / L / h. This provides a brand-new biomanufacturing platform for the industrial production of gastrodin.

The invention provides a genetic engineering strain, a construction method of the genetic engineering strain and application of the genetic engineering strain in biosynthesis of 5-hydroxytryptophan. According to the engineering bacterium, four key modules required by 5-HTP synthesis are sequentially introduced into a host genome in a genome integration mode through a CRISPR technology, and the four key modules comprise an aroGfbr-ppsA-tktA module for enhancing chorismate precursor supply, a trpEfbr-trpG module for catalyzing o-aminobenzoic acid generation, a salABCD gene cluster responsible for hydroxylating o-aminobenzoic acid into 5-hydroxy o-aminobenzoic acid, and a salABCD gene cluster responsible for hydroxylating 5-hydroxy o-aminobenzoic acid into 5-hydroxy o-aminobenzoic acid. And a trpDCBA module for converting the intermediate into the final product 5-HTP. The modules are respectively integrated into a plurality of non-essential gene regions, so that stable genetic expression is realized, and a complete metabolic pathway can be operated without plasmids or inducers. The strain can continuously accumulate 5-hydroxytryptophan (5-HTP) under a glucose fermentation condition, and has the advantages of simplicity and convenience in construction, high hereditary stability, low production cost, suitability for industrial-scale fermentation and the like.

Disclosed herein are compositions (e.g., pharmaceutical compositions, nutraceutical compositions) and methods useful for, inter alia, modulating levels of certain chorismate metabolites in a subject (e.g., in the gut of a subject). Modulation of levels of certain chorismate metabolites in a subject (e.g., levels of the chorismate metabolites in the gut) may have beneficial effects, prophylactic, and / or therapeutic effects for the subject, for example, in enhancing cognitive function or treating one or more signs or symptoms of a neurological disorder.

The application discloses a kind of recombinant escherichia coli and its construction method and application, the recombinant escherichia coli can be used for the production of L-phenylalanine.The application utilizes multiple rounds of mutagenesis screening to have high resistance to L-phenylalanine structural analogues, and has tyrosine auxotrophic mutant strain, and is regulated in the form of temperature-sensitive recombinant plasmid to code 3-deoxy-7-phosphoheptanoate synthase and branch acid mutase gene expression in L-phenylalanine synthesis pathway.The recombinant escherichia coli constructed in the application, in 5L fermenter, according to the cell growth stage adopts different temperature control and feeding mode, fermentation time is 56 h, under the condition that L-phenylalanine production is 62 g / L, sugar acid conversion rate is 25.2%, has the prospect and value of industrialization application.

The application discloses a high-yield resveratrol Yarrowia lipolytica engineering strain and construction and application thereof, and belongs to the technical field of synthetic biology and metabolic engineering. The Yarrowia lipolytica engineering strain provided by the application can realize high-yield resveratrol by using glucose as a substrate, and the yield of resveratrol reaches 3.157 g / L in shake flask fermentation, and the yield of resveratrol is increased to 30.7 g / L in 5L scale batch feeding fermentation, which is the highest value reported at present.