36 results about "Chorismic acid" patented technology

The invention discloses rapid sputum dissolving and storing liquid. The rapid sputum dissolving and storing liquid comprises the following components in parts by weight: ethanol 10-20 parts, glycerine 10-20 parts, vegetable oil 1-2 parts, chorismic acid 0.1-1 part, disodium EDTA (ethylene diamine tetraacetic acid) 0.01-0.2 part, SDS (sodium dodecyl sulfonate) 0.001-0.1 part, thiosugar 2-10 parts, and distilled water 50-80 parts. The rapid sputum dissolving and storing liquid can rapidly dissolve sputum and prevent mycobacterium tuberculosis from cracking; and meanwhile, the storage time of the dissolving and storing liquid is long, the rapid sputum dissolving and storing liquid can still be used after being continuously stored for 3-5 years, and the activity is not weakened obviously.

Provided is a microorganism that is able to efficiently produce protocatechuic acid or a salt thereof by using a saccharide as a raw material, and a method of efficiently producing protocatechuic acid or a salt thereof by using the microorganism. Provided is a transformant having protocatechuic acid producing ability, subjected to modifications of enhancement of 3-dehydroshikimate dehydratase activity; enhancement of chorismate pyruvate lyase activity; and enhancement of 4-hydroxybenzoate hydroxylase activity. Also provided is a method of producing protocatechuic acid or a salt thereof, including the step of culturing the transformant in a reaction solution containing a saccharide so as to cause the transformant to produce protocatechuic acid or a salt thereof.

The present invention relates to a polypeptide which belongs to the R2R3-type MYB family and which regulates the shikimate pathway towards the production of benzenoids. The shikimate pathway is a biosynthesis pathway through which the three essential aromatic amino acids tyrosine, phenylalanine and tryptophane are synthesized in plants, bacteria and fungi. The present inventiont provides for the first time a regulatory protein in the shikimate pathway and a means to regulate the biosynthesis of these three essential amino acids which cannot be produced by mammals. At the same time, it opens up the way for the regulation of the biosynthesis of aromatic and-non- aromatic compounds which are derived from these essential amino acids. A polypeptide or polynucleotide of the invention may be used in a method for manipulating the transcript levels of the genes of the shikimate pathway towards benzenoids for instance the genes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS), 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), L- phenylalanine ammonia-lyase (PAL) and chorismate mutase (CM). Through these enzymes, biosynthetic processes at lower levels may be influenced. For instance, compounds of the invention may be used in a method to regulate scent in flowers or to regulate resistance to pest insects or pathogenic organisms.

The invention discloses a genetically engineered strain for safe and efficient production of shenzamycin and its application; Shenzimycin metabolism gene knockout, chorismate metabolism gene knockout, point mutation method to reduce the biological activity of PheA, isozyme replacement method to reduce the biological activity of chorismate pyruvate lyase, increase the copy number of phzC gene in the genome, aroG promoter Replaced with a strong promoter Ptac to enhance the expression level of the DAHP synthase gene, the promoter replacement method to enhance the expression level of the sphenazimycin biosynthetic gene cluster and the expression level of the shenazinamycin efflux pump MexGHI-OpmD, to obtain; the bacterial strain can be safely , economical, super-high yield of Shenzimycin.

The invention discloses recombinant corynebacterium glutamicum capable of being used for highly yielding L-phenylalanine and a method for producing the L-phenylalanine (Phe) by fermentation and belongs to the field of metabolic engineering. According to the invention, a corynebacterium glutamicum engineering strain C.glutamicum19AF / 99TP capable of being used for highly yielding the L-Phe is obtained in a corynebacterium glutamicum type strain ATCC13032 by carrying out induction expression on the following four genes: a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase gene, a chorismate mutase / prephenate dehydratase gene which has resistance to feedback inhibition, a transketolase gene and a phosphoenolpyruvic acid synthetase gene. A shikimic acid metabolic pathway of an L-Phe synthetic metabolic pathway is over-expressed in the C.glutamicum ATCC13032; a key enzyme gene of a chorismic acid metabolic pathway enables the yield of the L-Phe to reach 3.47g / L; two enzyme genes which combines an expression center metabolic pathway to improve a precursor enables the highest yield of the L-Phe to reach 4.86g / L; and the original strain ATCC13032 which is used as a contrast strain cannot detect accumulation of the L-Phe in the integral fermenting process.

The invention relates to a marker-free gene deletion attenuated mutant strain of a Edwardsiella tarda wild strain. The marker-free gene deletion attenuated mutant strain is an attenuated live vaccine of an Edwardsiella tarda virulent strain, which deletes the chorismic acid synthase gene aroC of the Edwardsiella tarda virulent strain, three types of secretion system response element genes of eseB, escA, eseC and eseD and an endogenous plasmid, preferably, the Edwardsiella tarda virulent strain is a Edwardsiella tarda virulent strain EIB202 with the preservation number of CCTCC No:M208068; the endogenous plasmid is a plasmid of pEIB202; and the marker-free gene deletion attenuated mutant strain of the Edwardsiella tarda virulent strain is an attenuated strain WED with the preservation number of CCTCC No:M2010278. The invention also provides relevant preparations and application of the marker-free gene deletion attenuated mutant strain. The attenuated mutant strain or relevant preparations eliminate the potential environment and safety risk of products existing in the traditional attenuated live vaccines generally and is a safe, effective and economic vaccine aiming at Edwardsiella tarda diseases of cultured fishes.

The invention provides a preparation method of a formamide phenazine bio-fungicide. The preparation method comprises the following steps: firstly, implementing shake-flask culture on activated pseudomonas chlororaphis and pseudomonas aeruginosa, so as to obtain a seed solution A and a seed solution B; inoculating a fermentation medium with the seed solution A, conducting fermentation culture for 20h, inoculating the fermentation medium with the seed solution B, simultaneously refilling a nutrient solution, and continuing to implement fermentation culture for 40-50h, so that fermentation brothis obtained; centrifuging the fermentation broth at high speed, regulating pH value, and conducting extracting and concentrating, so as to a crude product; and implementing column chromatography and separating, so that the formamide phenazine bio-fungicide is obtained. The culture medium and culture conditions provided by the invention are quite suitable for growth of strains; the refilled nutrient solution contains chorismic acid; two strains undergo mixed fermentation; the content of formamide phenazine in the fermentation broth is obviously improved; based upon a series of post-treatment, afinished product that purity is higher than 99% is separated and obtained; and the finished product takes a strong inhibitory effect on pathogenic fungi of plants.

The invention discloses a chorismate mutase nucleotide sequence related to high yield of rice and application thereof. A method for screening rice with different yield traits provided by the inventioncomprises the following steps: (1) detecting the genotype based on a specific gene fragment, of the to-be-detected rice, wherein the specific gene fragment, OSCM3, is located in the rice genome, andhas two allelic forms: OSCM3_a and OSCM3_b; the OSCM3_a is shown by the sequence 1 of a sequence table; and the OSCM3_b is shown by the sequence 2 of the sequence table; and (2) judging in the following way: comparing tow genotypes in an adjoining growth condition, to determine that the average yield of the OSCM3_b homozygotic type rice population is higher than average yield of the OSCM3_a homozygotic type rice population. Experiments prove that the method provided by the invention can effectively screen the rice with different traits in yield per plant, and has the advantages of easiness inoperation and high accuracy, thereby having an important application value in rice breeding.