Rapid sputum dissolving and storing liquid

A technology for preserving liquid and sputum, applied in the preservation of microorganisms, biochemical equipment and methods, instruments, etc., can solve the problems of inability to quickly and accurately detect Mycobacterium tuberculosis, slow dissolution of sputum, etc. Simple preparation process

Active Publication Date: 2017-07-21
NANJING BANGBO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects in the prior art that the preservation solution dissolves sputum slowly and cannot quickly and accurately de...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A rapid sputum dissolution preservation solution, which is composed of the following components in parts by weight: 15 parts of ethanol, 15 parts of glycerin, 1.5 parts of peanut oil, 0.5 parts of chorismic acid, 0.1 part of disodium EDTA, 0.05 parts of SDS, 5-thio -6 parts of D-glucose, 60 parts of distilled water.

[0018] The sputum dissolution and preservation solution is stored at -4°C for 2 years, and its activity does not decrease, and it is stored at -20°C for 3 years, and its activity does not decrease.

Embodiment 2

[0020] A rapid sputum dissolution preservation solution, which is composed of the following components in parts by weight: 10 parts of ethanol, 10 parts of glycerin, 1 part of castor oil, 0.1 part of chorismic acid, 0.01 part of disodium EDTA, 0.001 part of SDS, dithio 2 parts of threitol, 50 parts of distilled water.

[0021] The sputum dissolution and preservation solution is stored at -4°C for 2 years, and its activity does not decrease, and it is stored at -20°C for 3 years, and its activity does not decrease.

Embodiment 3

[0023] A rapid sputum dissolution preservation solution, which is composed of the following components in parts by weight: 20 parts of ethanol, 20 parts of glycerin, 2 parts of cottonseed oil, 1 part of chorismic acid, 0.2 parts of disodium EDTA, 0.1 parts of SDS, glucosinolate glycosides 10 parts, distilled water 80 parts.

[0024] The sputum dissolution and preservation solution is stored at -4°C for 2 years, and its activity does not decrease, and it is stored at -20°C for 3 years, and its activity does not decrease.

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PUM

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Abstract

The invention discloses rapid sputum dissolving and storing liquid. The rapid sputum dissolving and storing liquid comprises the following components in parts by weight: ethanol 10-20 parts, glycerine 10-20 parts, vegetable oil 1-2 parts, chorismic acid 0.1-1 part, disodium EDTA (ethylene diamine tetraacetic acid) 0.01-0.2 part, SDS (sodium dodecyl sulfonate) 0.001-0.1 part, thiosugar 2-10 parts, and distilled water 50-80 parts. The rapid sputum dissolving and storing liquid can rapidly dissolve sputum and prevent mycobacterium tuberculosis from cracking; and meanwhile, the storage time of the dissolving and storing liquid is long, the rapid sputum dissolving and storing liquid can still be used after being continuously stored for 3-5 years, and the activity is not weakened obviously.

Description

technical field [0001] The invention belongs to the technical field of Mycobacterium tuberculosis detection, and in particular relates to a quick and convenient sputum dissolution preservation solution. Background technique [0002] Mycobacterium tuberculosis is the pathogenic bacteria that causes tuberculosis, the most common of which is pulmonary tuberculosis. Tuberculosis is still an important infectious disease. According to the WHO, about 8 million new cases occur each year and at least 3 million people die from the disease. Before the founding of the People's Republic of my country, the mortality rate reached 200-300 people / 100,000, ranking first among the causes of death from various diseases. Morbidity and mortality are greatly reduced. However, it should be noted that in some areas of the world, the incidence rate is on the rise again due to AIDS, drug abuse, application of immunosuppressants, alcoholism and poverty. [0003] Mycobacterium tuberculosis is a slend...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12N1/04C12R1/32
CPCC12N1/04C12Q1/04G01N2333/35
Inventor 王楚璇郭严沈瀚顾兵赵树立周玉程燕华
Owner NANJING BANGBO BIOTECH CO LTD
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