150 results about "Marker free" patented technology

A high-speed marker-free motion capture, which is capable of powerfully detecting a body's feature points corresponding to a body's end portions such as a head, hands, feet, trunk, arms and legs at a high speed in an illumination change or background or noises of cameras. The extracted feature points of the body can be directly tracked stably in a 3-dimensional space. The position errors of the feature points due to the change of the illumination conditions or a shadow can be automatically corrected and the feature points can be stably tracked with respect to overlapping and disappearance of the feature points. Further, when coordinates of the middle joints are estimated using 3-dimensional coordinates of the extracted feature points of the body, the present invention restores a human model by estimating the positions of the middle joints of the actor with high accuracy without using a motion database, thereby securing the stability and reality of the 3-dimensional motion data required in the motion capture.

In a method and apparatus for automatic marker-free fusion (matching) of 2D fluoroscopic C-arm images with preoperative 3D images using an intraoperatively acquired 3D data record, an intraoperative 3D image is obtained using a C-arm x-ray system, image-based matching of an existing preoperative 3D image in relation to the intraoperative 3D image is undertaken, which generates a matching matrix of a tool plate attached to the C-arm system is matched in relation to a navigation system, a 2D fluoroscopic image to be matched is obtained, with the C-arm of the C-arm system in any arbitrary location, a projection matrix for matching the 2D fluoroscopic image in relation to the 3D image is obtained, and the 2D fluoroscopic image is fused (matched) with the preoperative 3D image on the basis of the matching matrix and the projection matrix.

A method for estimating three-dimensional positions of human joints includes the steps of: a) marker-free motion capturing a moving figure for obtaining a multiview 2D image of the moving figure, and extracting a 2D feature point corresponding to a bodily end-effector; b) three-dimensionally matching the 2D feature point corresponding to the bodily end-effector, and recovering the 3D coordinates of the bodily end-effector; c) generating a 3D blob of the bodily end-effector, generating a virtual sphere with a radius that is a distance from a center of the 3D blob to a joint, and projecting the virtual sphere onto the obtained multiview 2D image of the moving figure; and d) detecting a coinciding point of a surface of the projected virtual sphere and the multiview 2D image of the moving figure, and estimating a 3D position corresponding to the coinciding point as a 3D position of the joint.

The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting an antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.

The invention belongs to the field of computer vision and computer graphics, and provides a method which can ensure a newly-generated video sequence to be vivid and reliable and solve the problem of high requirements on an experimental device. According to the technical scheme adopted by the invention, the realistic animation generation method based on Kinect uses a single Kinect v2.0 to carry out static three dimensional model and color depth skeleton information acquisition on a person. A marker-free motion capture method is used, skeleton motion information of each frame and a three dimensional model after deformation are obtained. After a user designates a new action, on the basis of the built data collection, a video for the new action by the person is generated through the proposed sparse representation-based low rank matrix filling texture synthesis method. The method of the invention is mainly applied to video processing.

The invention discloses a method for preparing an electrochemiluminescence biological sensing interface based on loaded graphite phase carbon nitride and an application thereof, and relates to the field of nano science, biological immunoassay, electrochemical sensing, etc. By means of the excellent electrochemiluminescence of a graphite phase carbon nitride nanosheet and the unique three-dimensional porous structure and good biocompatibility of nano porous gold, the graphite phase carbon nitride nanosheet is loaded in a nano porous gold skeleton for constructing a marker-free electrochemiluminescence biological sensing interface. Due to the three-dimensional porous structure, the luminescence property of the graphite phase carbon nitride nanosheet is greatly improved, and meanwhile, a biological recognition unit is fixed simply and quickly due to the good biocompatibility of the nano porous gold. The method is simple in steps and easy to operate, has relatively high luminescence stability and good response performance, and solves the problems of complicated steps and poor signal reproducibility of conventional methods. The method can be applicable to the preparation of a variety of electrochemiluminescence biological sensing interfaces of biomarkers, and has a wide application prospect in scientific research and clinical practice.

The invention discloses a method for detecting fungaltoxin through multiple signals. The method comprises the following steps: 1) combining fungaltoxin with aptamer: adding a to-be-detected sample after the reaction of the fungaltoxin aptamer and complementary sequence thereof, thereby acquiring a mixed solution A; 2) amplifying a digestion auxiliary signal: reacting the mixed solution A with restriction enzyme, thereby acquiring a mixed solution B; 3) preparing a guanine tetramer structure: reacting the mixed solution B with tail-end deoxynucleotide transferase and deoxyribonucleoside triphosphate and reacting with ligand molecules, thereby acquiring a mixed solution C; and 4) detecting and analyzing: performing catalytic oxidation reaction on the mixed solution C and different substrates, generating ultraviolet, fluorescent and chemiluminescent signals and calculating the content of fungaltoxin in the to-be-detected sample according to the relation of the response strength of all the signals and the fungaltoxin concentration. The method disclosed by the invention has the characteristics of quick and simple operation and treatment, short detection time, marker-free effect, low cost, high precision, high sensitivity, and the like.

Provided is an apparatus and method for detecting a horizon which is necessary to detect a camera movement when compositing sea images in a marker-free sea-image camera tracking system. In the method, an ROI is selected near a horizon in a still image of the moving image of the sea, and each maximum point corresponding to the maximum brightness difference is detected from brightness differences between two pixels of each pair having two symmetrical pixels in each column of the ROI. The horizon is detected through a line-fitting using the maximum points. Therefore, the result of horizon detection can be very stable and a horizon can be easily detected in a sea scene having hundreds of frames.

This algorithm provides a marker-free approach to establishing the pixel correspondence among the IR images taken at different times, which is the basis for quantitatively characterizing the variation of the heat energy and patterns pixel-wise on a breast surface. The idea is to use the corner points of the heat pattern and the branch points of the skeletons of the heat pattern on the body surface as the initial fiducial points for the longitudinal IR image registration. The Thin-Plate Spline technique is used to model the nonlinear deformation between two IR images taken at two different times. Mutual information between the TPS-transformed image and the target image is employed as the metric quantifying the quality of the longitudinal IR image registration. To optimize the registration, Nelder-Mead simplex method is used to locally modify the pairings of the fiducial points in the source and target IR images to maximize the mutual information.

The invention relates to a marker-free gene deletion attenuated mutant strain of an Edwardsiella tarda wild strain. The marker-free gene deletion attenuated mutant strain is an attenuated live vaccine of an Edwardsiella tarda virulent strain, which deletes the chorismic acid synthase gene aroC of the Edwardsiella tarda virulent strain, three types of secretion system response element genes of eseB, escA, eseC and eseD and an endogenous plasmid, preferably, the Edwardsiella tarda virulent strain is an Edwardsiella tarda virulent strain EIB202 with the preservation number of CCTCC No:M208068; the endogenous plasmid is a plasmid of pEIB202; and the marker-free gene deletion attenuated mutant strain of the Edwardsiella tarda virulent strain is an attenuated strain WED with the preservation number of CCTCC No:M2010278. The invention also provides relevant preparations and application of the marker-free gene deletion attenuated mutant strain. The attenuated mutant strain or relevant preparations eliminate the potential environment and safety risk of products existing in the traditional attenuated live vaccines generally and is a safe, effective and economic vaccine aiming at Edwardsiella tarda diseases of cultured fishes.

The invention provides a coronene derivative probe and a preparation method thereof and a protein detection method based on the coronene derivative probe and aptamer. The objective of the invention is to overcome the problems of tedious operation, complex process and low sensitivity of a conventional protein detection method. The probe is 1,2,7,8-tetra-(4-trimethylamine butyloxycarbonyl)-coronene and has four charges and water solubility. The invention provides the preparation method for the coronene derivative probe. The invention further provides the protein detection method based on the coronene derivative probe and the aptamer. The protein detection method is marker-free, simple and fast and does not need covalent modification; moreover, a fluorescence enhanced signal is given out in detection, so compared with a fluorescence weakened signal, higher sensitivity is obtained.

The invention provides a deconvolution-guided semi-supervised plant leaf disease identification and segmentation method, which uses a small amount of disease category labels and disease spot pixel-level labels to achieve disease category identification and disease spot region segmentation through deconvolution. According to the method, a category prediction label of an unmarked sample is generatedthrough a consistency regularization and entropy minimization method; image mixing is carried out on the marked sample and the unmarked sample, and semi-supervised disease classification is carried out by utilizing the newly generated image; and up-sampling is performed on the category information, and semi-supervised scab segmentation is performed by using a small number of pixel-level marks. Inthe process of model training, model parameters are updated by using exponential weighted average, so that the model is more robust in test data. The method is suitable for identifying and segmentingplant leaf diseases with insufficient label samples, integration of identification and segmentation is achieved, the model has high generalization capacity in leaf images with insufficient light andforeign matter shielding, and the identification and segmentation speed can meet the real-time requirement.

The invention discloses a silver nanocluster fluorescent probe based T4 polynucleotide kinase activity detecting method. The silver nanocluster fluorescent probe based T4 polynucleotide kinase activity detecting method is characterized in that a marker-free 'turn-off' type fluorescent probe is manufactured for detecting activity of T4 PNK. A DNA chain for preparing silver nanocluster template is S1(DNA S1), and a DNA chain containing [3'-G4(TG4)2TG3] sequence is S2(DNA S2), and the two chains are hybridized; under action of a G-rich sequence, a silver nanocluster can generate strong red fluorescence, and 5-hydroxyl terminal of the double chains DNA is phosphorylated through T4 PNK, so that a 5-phosphoryl group terminal is generated; the 5-phosphoryl group terminal can be quickly distinguished and degraded by gamma exonuclease, so that two DNA chains are separated, the fluorescence signal is quickly weakened to generate a signal which is in inverse proportion with T4 PNK concentration.Therefore, the silver nanocluster fluorescent probe based T4 polynucleotide kinase activity detecting method can be used for precisely detecting activity of T4 PNK; and the detecting sensitivity of the method can be 0.01 U/mL according to the strength of the DNA-AgNCs fluorescence signal.

The invention relates to a preparing method of a micro-fluidic chip with embedded hyaluronic acid functionalized nanofiber membrane. The preparing method comprises the steps that chitosan/PEO spinningsolution is subjected to static spinning, vacuum drying and crosslinking, and chitosan nanofiber membrane CNFs is obtained; zwitter-ions CBAA and targeting ligands HA-Cys-MPTMS are modified, and HA functionalized nanofiber membrane HA-CBAA-CNFs is obtained; the nanofiber membrane HA-CBAA-CNFs and a PDMS micro-fluidic channel cover plate are subjected to plasma bonding, and the micro-fluidic chipwith embedded hyaluronic acid(HA) functional nanofiber membrane is obtained, wherein the micro-fluidic chip can be used for sorting and non-destructive releasing of circulating tumor cells. The preparing method has the advantages that the method is simple in technology, easy to operate and high in specificity, marker-free and high-throughput sorting and non-destructive releasing of the circulatingtumor cells can be completed in a short time, accordingly efficient separation for the circulating tumor cells is achieved, and the preparing method has good application prospects in early detectionof tumor patients.

A marker-free motion capture apparatus having a function of correcting a tracking error and a method thereof are disclosed. The apparatus includes: a grouping unit for grouping feature candidates located within a threshold distance on a three-dimensional space at a previous time; a feature point selecting unit for generating a first curve connecting a predetermined number of feature points, and selecting a feature candidate closest to the first curve as a feature point of a previous time; a feature point correcting unit for generating a second curve connecting a predetermined number of feature points including the feature point of a previous time, and correcting a feature point of a current time calculated based on a Kalman filtering scheme using the second curve; and a controlling unit for calculating a location of a feature point of each time using a Kalman filtering scheme and generally controlling the marker-free motion capture apparatus.

The invention relates to a method for the selection and identification of at least on representative (interaction partner) from a plurality of peptide or protein molecules, which can specifically interact with at least one representative from a plurality of target molecules, forming a bond. The inventive method comprises the following steps: (a) a virus system consisting of a plurality of viruses, wherein each virus respectively presents at least on representative from the plurality of peptide or protein molecules on the surface thereof, is brought into contact with the plurality of target molecules (ligands) which are immobilized on the surface of a solid phase carrier such that they are position addressable in a two-dimensional grade; (b) unbound viruses removed from the surface thereof; and (c) the interaction partner is identified by detection and determination of the position of the bond between the immobilized ligand and the interaction partner presented by the virus with the aid of a marker-free detection method. The described method makes it possible to concentrate viruses presenting interaction partners by means of an optionally cyclic repetition of selection. Optionally, selected interaction partners are recombinantly expressed after identification of the coding nucleotide sequence.

The invention belongs to the fields of nano material and biosensing. The invention provides a prussian blue cube / molybdenum disulfide nano composite material and preparation method and application thereof. The nano composite material uses polyvinylpyrrolidone as a surfactant and reducing agent and uses halogen as a morphology control agent. A molybdenum disulfide solution, a potassium ferricyanide solution and molybdenum disulfide solution and a ferric trichloride solution are mixed, subjected to a wet chemical reduction method, and purified to obtain the prussian blue cube / molybdenum disulfide nano composite material. The composite material can be used to construct a marker-free immune sensor. The composite material has good dispersion and biocompatibility and is prepared by wet chemical one-step reduction method. The composite material has a fast and efficient catalytic performance, and great potential applications in electrocatalysis and electrochemical sensor of the electrochemical field; the composite material can be used for constructing marker-free electrochemical immunosensor to achieve high sensitivity and specificity detection of tumor marker.

The invention provides a transgenic carrier with function of automatic control and elimination of selection marker and application thereof in zea mays marker-free transgenic breeding, and belongs to the technical field of breeding of zea mays molecules. The carrier uses a pEGAD carrier as a skeleton carrier, and a fusion gene of EGFP (enhanced green fluorescent protein) and IPT (isopentenyl transferase) genes is used for replacing a bar gene and is used as the selection marker; an expression framework of the selection marker is integrated into a Cre/lox site specific recombination system. Theapplication comprises the following steps of transferring the carrier into a zea mays embryonic callus, obtaining the zea mays embryonic callus with the green fluorescent expression effect; after heatshock, culturing the zea mays embryonic callus without the green fluorescent expression effect by differentiating and rooting, so as to obtain a transgenic plant. The carrier has the advantages thatthe selection efficiency is improved, the removal of the transgene selection marker is realized, and the theoretical and application basis is provided for the large-range development of marker-free zea mays transgene.

The present invention relates to a method for analyzing chromosomes by preparing a chromosome preparation, measuring at least one interference property of the chromosome preparation and characterizing at least one chromosome structure by way of the interference property. Also, the invention relates to the use of a near field microscope for analyzing unstained chromosomes.