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160 results about "Marker free" patented technology
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Apparatus and method for high-speed marker-free motion capture
InactiveUS7239718B2Stable trackingEasily color differenceImage enhancementImage analysisComputer graphics (images)Three-dimensional space
A high-speed marker-free motion capture, which is capable of powerfully detecting a body's feature points corresponding to a body's end portions such as a head, hands, feet, trunk, arms and legs at a high speed in an illumination change or background or noises of cameras. The extracted feature points of the body can be directly tracked stably in a 3-dimensional space. The position errors of the feature points due to the change of the illumination conditions or a shadow can be automatically corrected and the feature points can be stably tracked with respect to overlapping and disappearance of the feature points. Further, when coordinates of the middle joints are estimated using 3-dimensional coordinates of the extracted feature points of the body, the present invention restores a human model by estimating the positions of the middle joints of the actor with high accuracy without using a motion database, thereby securing the stability and reality of the 3-dimensional motion data required in the motion capture.
Owner:ELECTRONICS & TELECOMM RES INST
Method for marker-free automatic fusion of 2-D fluoroscopic C-arm images with preoperative 3D images using an intraoperatively obtained 3D data record
In a method and apparatus for automatic marker-free fusion (matching) of 2D fluoroscopic C-arm images with preoperative 3D images using an intraoperatively acquired 3D data record, an intraoperative 3D image is obtained using a C-arm x-ray system, image-based matching of an existing preoperative 3D image in relation to the intraoperative 3D image is undertaken, which generates a matching matrix of a tool plate attached to the C-arm system is matched in relation to a navigation system, a 2D fluoroscopic image to be matched is obtained, with the C-arm of the C-arm system in any arbitrary location, a projection matrix for matching the 2D fluoroscopic image in relation to the 3D image is obtained, and the 2D fluoroscopic image is fused (matched) with the preoperative 3D image on the basis of the matching matrix and the projection matrix.
Owner:SIEMENS HEALTHCARE GMBH
Method for marker-free automatic fusion of 2-D fluoroscopic C-arm images with preoperative 3D images using an intraoperatively obtained 3D data record
ActiveUS20050004454A1Simple wayImage enhancementMaterial analysis using wave/particle radiationHat matrixFluoroscopic image
In a method and apparatus for automatic marker-free fusion (matching) of 2D fluoroscopic C-arm images with preoperative 3D images using an intraoperatively acquired 3D data record, an intraoperative 3D image is obtained using a C-arm x-ray system, image-based matching of an existing preoperative 3D image in relation to the intraoperative 3D image is undertaken, which generates a matching matrix of a tool plate attached to the C-arm system is matched in relation to a navigation system, a 2D fluoroscopic image to be matched is obtained, with the C-arm of the C-arm system in any arbitrary location, a projection matrix for matching the 2D fluoroscopic image in relation to the 3D image is obtained, and the 2D fluoroscopic image is fused (matched) with the preoperative 3D image on the basis of the matching matrix and the projection matrix.
Owner:SIEMENS HEALTHCARE GMBH
Method for estimating three-dimensional position of human joint using sphere projecting technique
InactiveUS20070126743A1Accurately determineAccurate detectionImage enhancementImage analysisGraphicsActuator
A method for estimating three-dimensional positions of human joints includes the steps of: a) marker-free motion capturing a moving figure for obtaining a multiview 2D image of the moving figure, and extracting a 2D feature point corresponding to a bodily end-effector; b) three-dimensionally matching the 2D feature point corresponding to the bodily end-effector, and recovering the 3D coordinates of the bodily end-effector; c) generating a 3D blob of the bodily end-effector, generating a virtual sphere with a radius that is a distance from a center of the 3D blob to a joint, and projecting the virtual sphere onto the obtained multiview 2D image of the moving figure; and d) detecting a coinciding point of a surface of the projected virtual sphere and the multiview 2D image of the moving figure, and estimating a 3D position corresponding to the coinciding point as a 3D position of the joint.
Owner:ELECTRONICS & TELECOMM RES INST
Marker free transgenic plants engineering the chloroplast genome without the use of antibiotic selection
InactiveUS20020137214A1Increased riskReduce development of resistanceOther foreign material introduction processesOxidoreductasesAntibiotic freeChloroplast
The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting an antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.
Owner:UNIV OF CENT FLORIDA +1
Realistic animation generation method based on Kinect
ActiveCN104915978AStrong sense of realityExperimental conditions are simpleAnimationData setSomatosensory system
The invention belongs to the field of computer vision and computer graphics, and provides a method which can ensure a newly-generated video sequence to be vivid and reliable and solve the problem of high requirements on an experimental device. According to the technical scheme adopted by the invention, the realistic animation generation method based on Kinect uses a single Kinect v2.0 to carry out static three dimensional model and color depth skeleton information acquisition on a person. A marker-free motion capture method is used, skeleton motion information of each frame and a three dimensional model after deformation are obtained. After a user designates a new action, on the basis of the built data collection, a video for the new action by the person is generated through the proposed sparse representation-based low rank matrix filling texture synthesis method. The method of the invention is mainly applied to video processing.
Owner:深圳市凌云视迅科技有限责任公司
Method for preparing electrochemiluminescence biological sensing interface based on loaded graphite phase carbon nitride and application thereof
ActiveCN104677892ASimple stepsEasy to operateChemiluminescene/bioluminescenceBiological testingCarbon nitrideElectrochemiluminescence
The invention discloses a method for preparing an electrochemiluminescence biological sensing interface based on loaded graphite phase carbon nitride and an application thereof, and relates to the field of nano science, biological immunoassay, electrochemical sensing, etc. By means of the excellent electrochemiluminescence of a graphite phase carbon nitride nanosheet and the unique three-dimensional porous structure and good biocompatibility of nano porous gold, the graphite phase carbon nitride nanosheet is loaded in a nano porous gold skeleton for constructing a marker-free electrochemiluminescence biological sensing interface. Due to the three-dimensional porous structure, the luminescence property of the graphite phase carbon nitride nanosheet is greatly improved, and meanwhile, a biological recognition unit is fixed simply and quickly due to the good biocompatibility of the nano porous gold. The method is simple in steps and easy to operate, has relatively high luminescence stability and good response performance, and solves the problems of complicated steps and poor signal reproducibility of conventional methods. The method can be applicable to the preparation of a variety of electrochemiluminescence biological sensing interfaces of biomarkers, and has a wide application prospect in scientific research and clinical practice.
Owner:UNIV OF JINAN
System and method for producing a label-free micro-array biochip
InactiveUS20090181857A1Reduce sampling costsLow costLibrary screeningMaterial analysis by optical meansFluorescenceSurface plasmonic resonance
A system and method for producing label-free micro-array biochip based on the surface plasmon resonance in metallic nano-slit arrays, wherein the micro-array biochip of the does not utilize fluorescent labeling. Without the fluorescence labeling, the label-free micro-array substantially reduces the sample cost and can detect bio-molecular interactions in their native forms.
Owner:ACAD SINIC
Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
Owner:NOVOZYMES INC
Method for detecting fungaltoxin through multiple signals and kit
ActiveCN107271668ARich identification methodsRich Signal TransformationBiological material analysisBiological testingFluorescenceUltraviolet
The invention discloses a method for detecting fungaltoxin through multiple signals. The method comprises the following steps: 1) combining fungaltoxin with aptamer: adding a to-be-detected sample after the reaction of the fungaltoxin aptamer and complementary sequence thereof, thereby acquiring a mixed solution A; 2) amplifying a digestion auxiliary signal: reacting the mixed solution A with restriction enzyme, thereby acquiring a mixed solution B; 3) preparing a guanine tetramer structure: reacting the mixed solution B with tail-end deoxynucleotide transferase and deoxyribonucleoside triphosphate and reacting with ligand molecules, thereby acquiring a mixed solution C; and 4) detecting and analyzing: performing catalytic oxidation reaction on the mixed solution C and different substrates, generating ultraviolet, fluorescent and chemiluminescent signals and calculating the content of fungaltoxin in the to-be-detected sample according to the relation of the response strength of all the signals and the fungaltoxin concentration. The method disclosed by the invention has the characteristics of quick and simple operation and treatment, short detection time, marker-free effect, low cost, high precision, high sensitivity, and the like.
Owner:ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
Apparatus and method for detecting horizon in sea image
Provided is an apparatus and method for detecting a horizon which is necessary to detect a camera movement when compositing sea images in a marker-free sea-image camera tracking system. In the method, an ROI is selected near a horizon in a still image of the moving image of the sea, and each maximum point corresponding to the maximum brightness difference is detected from brightness differences between two pixels of each pair having two symmetrical pixels in each column of the ROI. The horizon is detected through a line-fitting using the maximum points. Therefore, the result of horizon detection can be very stable and a horizon can be easily detected in a sea scene having hundreds of frames.
Owner:ELECTRONICS & TELECOMM RES INST
Longitudinal Image Registration Algorithm For Infrared Images For Chemotherapy Response Monitoring And Early Detection Of Breast Cancers
ActiveUS20130004035A1Maximizing mutual informationMore informationImage enhancementImage analysisNonlinear deformationMutual information
This algorithm provides a marker-free approach to establishing the pixel correspondence among the IR images taken at different times, which is the basis for quantitatively characterizing the variation of the heat energy and patterns pixel-wise on a breast surface. The idea is to use the corner points of the heat pattern and the branch points of the skeletons of the heat pattern on the body surface as the initial fiducial points for the longitudinal IR image registration. The Thin-Plate Spline technique is used to model the nonlinear deformation between two IR images taken at two different times. Mutual information between the TPS-transformed image and the target image is employed as the metric quantifying the quality of the longitudinal IR image registration. To optimize the registration, Nelder-Mead simplex method is used to locally modify the pairings of the fiducial points in the source and target IR images to maximize the mutual information.
Owner:NAT TAIWAN UNIV
Marker-free gene deletion attenuated mutant strain of Edwardsiella tarda wild strain as well as relevant preparations and application thereof
ActiveCN101974472AGood control effectImprove immunityAntibacterial agentsBacterial antigen ingredientsChorismic acidAttenuated Live Vaccine
The invention relates to a marker-free gene deletion attenuated mutant strain of an Edwardsiella tarda wild strain. The marker-free gene deletion attenuated mutant strain is an attenuated live vaccine of an Edwardsiella tarda virulent strain, which deletes the chorismic acid synthase gene aroC of the Edwardsiella tarda virulent strain, three types of secretion system response element genes of eseB, escA, eseC and eseD and an endogenous plasmid, preferably, the Edwardsiella tarda virulent strain is an Edwardsiella tarda virulent strain EIB202 with the preservation number of CCTCC No:M208068; the endogenous plasmid is a plasmid of pEIB202; and the marker-free gene deletion attenuated mutant strain of the Edwardsiella tarda virulent strain is an attenuated strain WED with the preservation number of CCTCC No:M2010278. The invention also provides relevant preparations and application of the marker-free gene deletion attenuated mutant strain. The attenuated mutant strain or relevant preparations eliminate the potential environment and safety risk of products existing in the traditional attenuated live vaccines generally and is a safe, effective and economic vaccine aiming at Edwardsiella tarda diseases of cultured fishes.
Owner:EAST CHINA UNIV OF SCI & TECH
Coronene derivative probe and preparation method thereof, and protein detection method based on coronene derivative probe and aptamer
ActiveCN103992788AGood water solubilityEasy to prepareOrganic compound preparationFluorescence/phosphorescenceSolubilityProtein detection
The invention provides a coronene derivative probe and a preparation method thereof and a protein detection method based on the coronene derivative probe and aptamer. The objective of the invention is to overcome the problems of tedious operation, complex process and low sensitivity of a conventional protein detection method. The probe is 1,2,7,8-tetra-(4-trimethylamine butyloxycarbonyl)-coronene and has four charges and water solubility. The invention provides the preparation method for the coronene derivative probe. The invention further provides the protein detection method based on the coronene derivative probe and the aptamer. The protein detection method is marker-free, simple and fast and does not need covalent modification; moreover, a fluorescence enhanced signal is given out in detection, so compared with a fluorescence weakened signal, higher sensitivity is obtained.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
Deconvolution guided semi-supervised plant leaf disease identification and segmentation method
PendingCN112036335ARealize integrationMeet real-time requirementsCharacter and pattern recognitionNeural architecturesPattern recognitionDisease classification
The invention provides a deconvolution-guided semi-supervised plant leaf disease identification and segmentation method, which uses a small amount of disease category labels and disease spot pixel-level labels to achieve disease category identification and disease spot region segmentation through deconvolution. According to the method, a category prediction label of an unmarked sample is generatedthrough a consistency regularization and entropy minimization method; image mixing is carried out on the marked sample and the unmarked sample, and semi-supervised disease classification is carried out by utilizing the newly generated image; and up-sampling is performed on the category information, and semi-supervised scab segmentation is performed by using a small number of pixel-level marks. Inthe process of model training, model parameters are updated by using exponential weighted average, so that the model is more robust in test data. The method is suitable for identifying and segmentingplant leaf diseases with insufficient label samples, integration of identification and segmentation is achieved, the model has high generalization capacity in leaf images with insufficient light andforeign matter shielding, and the identification and segmentation speed can meet the real-time requirement.
Owner:NANJING AGRICULTURAL UNIVERSITY
Micro-array biochip, and preparation method and application of micro-array biochip
InactiveCN104897617AIncrease fixed amountPrecisely control the amount of protein immobilizationMaterial analysis by optical meansBiological testingCross linkerBiology
The invention relates to a micro-array biochip, and a preparation method and an application of the micro-array biochip. The micro-array biochip comprises a glass chip substrate and a modification layer. The modification layer comprises a chromium layer, a silver and / or gold layer, a self-assembly monomolecular layer, and a photo-cross-linking agent and high-molecular layer, which are overlaid in sequential on the surface of the glass chip substrate. The invention also provides a protein micro-array, a method for preparing the protein micro-array, and an application of the protein micro-array to detect micromolecule medicine. According to the invention, three-dimensional high-molecular surface modification of the micro-array biochip is completed by the method, and the fixation amount of protein on the surface of the micro-array biochip can be effectively improved, so that micromolecule, hard to detect in the prior art, can be detected without marking, in accuracy, and in high flux. The micro-array biochip is of great significance to the field, such as molecular biology study and medicinal development.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Recombinant Salmonella choleraesuis for expressing surface antigen gene sao of streptococcus suis type 2, vaccine and application
ActiveCN101979501AGood immune protectionPreserve immune efficiencyAntibacterial agentsBacterial antigen ingredientsBacteroidesAntigen
The invention belongs to the field of animal bacterium gene engineering, and in particular relates to construction of resistance marker-free recombinant Salmonella choleraesuis for expressing surface antigen sao gene segment of streptococcus suis type 2, preparation of a vaccine and application. The resistance marker-free recombinant Salmonella choleraesuis for expressing the surface antigen sao gene segment of the streptococcus suis type 2, namely asd-C500 / Pya-saoA is obtained, and the collection number is CCTCC NO: M2010156. The asd gene of the Salmonella choleraesuis is deleted in the recombinant strain, and the recombinant strain contains plasmid pYA-saoA capable of expressing the asd and the sao gene segment of the streptococcus suis type 2. The invention also discloses a method for preparing the recombinant strain and the vaccine, and application in preparing Salmonella choleraesuis-streptococcus suis type 2 vaccines.
Owner:HUAZHONG AGRI UNIV +1
Method for classifying N central points based on gene expression programming
InactiveCN104699804AImprove accuracyImprove operational efficiencyRelational databasesSpecial data processing applicationsData setAlgorithm
The invention discloses a method for classifying N central points based on gene expression programming and belongs to the technical field of machine learning. The method includes the steps of firstly, partitioning to-be-classified training data into a classification-marker-contained data set and a classification-marker-free data set; secondly, searching classification central points from a multi-dimensional space of the classification-marker-free data set according to a distance formula; thirdly, assessing accuracy of the searched classification central points on the classification-marker-contained data set; fourthly, using a gene expression programming method for expressing, searching and updating the classification central points to obtain new classification central points; fifthly, classifying unknown points according to the distances between the unknown points and the new classification central points; sixthly, repeating the third step, the fourth step and the fifth step until an objective function satisfies stop conditions. By the aid of the method for classifying the N central points based on gene expression programming, calculated quantity can be decreased while susceptibility to unbalanced data is avoided.
Owner:ZHEJIANG UNIV OF TECH
Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
InactiveUS20040231015A1Other foreign material introduction processesOxidoreductasesAntibiotic freeChloroplast
The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting a antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.
Owner:AUBURN UNIV +1
Silver nanocluster fluorescent probe based T4 polynucleotide kinase activity detecting method
InactiveCN108823280AWide detection rangeLow detection limitMicrobiological testing/measurementFluorescence/phosphorescenceT4 polynucleotide kinaseNucleotide kinase activity
Owner:THE SECOND HOSPITAL OF NANJING
Preparing method of micro-fluidic chip with embedded hyaluronic acid functionalized nanofiber membrane
ActiveCN107653575ALow costReduce non-specific adsorptionCell culture supports/coatingTumor/cancer cellsTumor cellsTumor patient
The invention relates to a preparing method of a micro-fluidic chip with embedded hyaluronic acid functionalized nanofiber membrane. The preparing method comprises the steps that chitosan / PEO spinningsolution is subjected to static spinning, vacuum drying and crosslinking, and chitosan nanofiber membrane CNFs is obtained; zwitter-ions CBAA and targeting ligands HA-Cys-MPTMS are modified, and HA functionalized nanofiber membrane HA-CBAA-CNFs is obtained; the nanofiber membrane HA-CBAA-CNFs and a PDMS micro-fluidic channel cover plate are subjected to plasma bonding, and the micro-fluidic chipwith embedded hyaluronic acid(HA) functional nanofiber membrane is obtained, wherein the micro-fluidic chip can be used for sorting and non-destructive releasing of circulating tumor cells. The preparing method has the advantages that the method is simple in technology, easy to operate and high in specificity, marker-free and high-throughput sorting and non-destructive releasing of the circulatingtumor cells can be completed in a short time, accordingly efficient separation for the circulating tumor cells is achieved, and the preparing method has good application prospects in early detectionof tumor patients.
Owner:DONGHUA UNIV
No mark gene deletion deoxidated mutant strain of wild Manhu bacteria and its use
InactiveCN1644680AImprove immunityGood control effectAntibacterial agentsBacteriaVibrio anguillarumFish farming
A bacterin for fish farming is used to treat vibriosis with reduced toxicity. The vaccine is mainly formed by no marker gene deletion strains with toxicity reduced. It is effective for cross immunization of Rongzao vibrios. The vaccine made from it can effectively be used to prevent testing fishes from diseases caused by vibriosis.
Owner:SHANGHAI HAOSI MARINE BIOTECHNOLOGY CO LTD
Optimized dual T-DNA expression vector obtaining marker-free genetically modified organisms (GMOs) and applications thereof
ActiveCN103898135AReduce Chain RatioAvoid form read-throughVector-based foreign material introductionAngiosperms/flowering plantsHead to headBiology
The invention discloses an optimized dual T-DNA expression vector obtaining marker-free genetically modified organisms (GMOs) and applications thereof. The dual T-DNA expression vector A provided by the invention is prepared by inserting a dual T-DNA region into an expression vector; wherein the dual T-DNA region comprises two individual T-DNA regions: a T-DNA A region and a T-DNA B region; the right boundary of the T-DNA A region is next to the right boundary of the T-DNA B region, and the left boundary of the T-DNA A region is far away from the left boundary of the T-DNA B region. Experiment results have shown that: in the constructed dual T-DNA plasmid, two right boundaries of the two T-DNA regions get close to each other in a plasmid ring so as to form a head-to-head structure (LB-RB-RB-LR type); thus readthrough in the transcription process is avoided, the ratio of interlocked transforming genes and selective marker genes is reduced, and finally the probability that marker-free transgenic strains are separated from the T1 generation is improved, so the expression vector has a wide application prospect.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Marker-free motion capture apparatus and method for correcting tracking error
InactiveUS20060126895A1Stable and fastDetects tracking errorImage enhancementImage analysisThree-dimensional spaceMotion capture
A marker-free motion capture apparatus having a function of correcting a tracking error and a method thereof are disclosed. The apparatus includes: a grouping unit for grouping feature candidates located within a threshold distance on a three-dimensional space at a previous time; a feature point selecting unit for generating a first curve connecting a predetermined number of feature points, and selecting a feature candidate closest to the first curve as a feature point of a previous time; a feature point correcting unit for generating a second curve connecting a predetermined number of feature points including the feature point of a previous time, and correcting a feature point of a current time calculated based on a Kalman filtering scheme using the second curve; and a controlling unit for calculating a location of a feature point of each time using a Kalman filtering scheme and generally controlling the marker-free motion capture apparatus.
Owner:ELECTRONICS & TELECOMM RES INST
Fluorescent biosensor, preparation method and application of fluorescent biosensor to detect organic phosphorus pesticide
ActiveCN107937480ADetection sensitivityDetection specificityMicrobiological testing/measurementBiological material analysisTyrosineOrganophosphorus pesticides
The invention provides a fluorescent biosensor, a preparation method and an application of the fluorescent biosensor to detect an organic phosphorus pesticide. The luminous effect of copper nanoparticles which take polythymine-carrying single-chain DNA as a template is used, tyrosinase is used to quench the copper nanoparticles, and the organic phosphorus pesticide is added to inhibit the activityof tyrosinase, thereby preparing the fluorescent biosensor which is constructed based on copper nanoparticles which take polythymine-carrying DNA as a template, tyrosinase and the organic phosphoruspesticide. The fluorescent biosensor can detect the organic phosphorus pesticide with sensitivity and specificity. Compared with a fluorescent biosensor in the prior art, the fluorescent biosensor adopts unmarked DNA, is simple to operate and low in cost, and is free of any chemical mark and modification. Through enzymatic activity inhibition, a sensor capable of detecting an organic phosphorus pesticide can be prepared. The result shows that the sensor can detect the organic phosphorus pesticide with the sensitivity being 0.1 ng / L to 1000 ng / L. The fluorescent biosensor is simple to operate,high in sensitivity and low in detection limit.
Owner:ANHUI NORMAL UNIV
Methods and compositions for marker-free genome modification
Compositions and methods are provided for modifying a nucleotide sequence in the genome of a plant cell, without the use of a selectable marker. The methods and compositions employ a guide polynucleotide / Cas endonuclease system to make a double strand break in a target site located in a nucleotide sequence and plant cells are obtained without the use of a selectable marker, and to provide an effective system for modifying target sites within the genome of a plant, plant cell or seed. Compositions and methods are also provided for producing a plant cell, callus tissue or plant having a modified nucleotide sequence in its genome, without the use of a selectable marker.
Owner:PIONEER HI BRED INT INC
Method for the selection and identification of peptide or protein molecules by means of phage display
InactiveUS20050014135A1Facilitate conductionMaterial nanotechnologyMicrobiological testing/measurementProtein moleculesNucleotide
The invention relates to a method for the selection and identification of at least on representative (interaction partner) from a plurality of peptide or protein molecules, which can specifically interact with at least one representative from a plurality of target molecules, forming a bond. The inventive method comprises the following steps: (a) a virus system consisting of a plurality of viruses, wherein each virus respectively presents at least on representative from the plurality of peptide or protein molecules on the surface thereof, is brought into contact with the plurality of target molecules (ligands) which are immobilized on the surface of a solid phase carrier such that they are position addressable in a two-dimensional grade; (b) unbound viruses removed from the surface thereof; and (c) the interaction partner is identified by detection and determination of the position of the bond between the immobilized ligand and the interaction partner presented by the virus with the aid of a marker-free detection method. The described method makes it possible to concentrate viruses presenting interaction partners by means of an optionally cyclic repetition of selection. Optionally, selected interaction partners are recombinantly expressed after identification of the coding nucleotide sequence.
Owner:SANTHERA PHARMA SCHWEIZ
Preparation method of prussian blue cube / molybdenum disulfide nano composite material
ActiveCN105842438AOvercome the disadvantage of poor stabilityQuick tagMaterial analysis by electric/magnetic meansBiological testingActive agentPotassium ferricyanide
The invention belongs to the fields of nano material and biosensing. The invention provides a prussian blue cube / molybdenum disulfide nano composite material and preparation method and application thereof. The nano composite material uses polyvinylpyrrolidone as a surfactant and reducing agent and uses halogen as a morphology control agent. A molybdenum disulfide solution, a potassium ferricyanide solution and molybdenum disulfide solution and a ferric trichloride solution are mixed, subjected to a wet chemical reduction method, and purified to obtain the prussian blue cube / molybdenum disulfide nano composite material. The composite material can be used to construct a marker-free immune sensor. The composite material has good dispersion and biocompatibility and is prepared by wet chemical one-step reduction method. The composite material has a fast and efficient catalytic performance, and great potential applications in electrocatalysis and electrochemical sensor of the electrochemical field; the composite material can be used for constructing marker-free electrochemical immunosensor to achieve high sensitivity and specificity detection of tumor marker.
Owner:NANJING UNIV OF POSTS & TELECOMM
Method for estimating three-dimensional position of human joint using sphere projecting technique
InactiveUS7869646B2Accurately determineAccurate detectionImage enhancementImage analysisGraphicsActuator
Owner:ELECTRONICS & TELECOMM RES INST
Methods and compositions for obtaining marker-free transgenic plants
The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
Owner:MONSANTO TECH LLC
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