78results about How to "Increase fixed amount" patented technology

The invention discloses a preparation method of a multi-growth-factor slow release coating of a titanium and titanium alloy surface. According to the method, a plurality of layers of coatings containing nanoparticles of growth factors are prepared on the titanium surface by taking the nanoparticles as a growth factor carrier and charged high molecular thin films as a medium. The nanoparticles included in the coating are prepared by mixing polycation solution containing dopamine of certain concentration with polyanion solution containing the growth factors. The nanoparticles have good dispersibility, can protect the activity of the growth factors and are easy to fix on the surface of a material. By controlling the electric property of the nanoparticles and the high molecular thin films, nanoparticles carrying with different factors are embedded between the high molecular thin films. By adjusting the loading quantity and the loading sequence of different nanoparticles and the thickness of the coatings, effective control over release speed, release quantity and release sequence of the various growth factors can be realized.

The invention belongs to the technical field of analytical chemistry and chemical sensors and discloses an electrochemical immunosensor for detecting a toxoplasma gondii IgM (Immunoglobulin m) antibody (Tg-IgM) of a gravida and a preparation method of the electrochemical immunosensor. The immunosensor is prepared by sequentially modifying graphene, polythionine, gold nanoparticles and capture antigen to the surface of a glassy carbon electrode. An enzyme-functionalized nano-composite detection probe with an electrical signal amplifying function is prepared by assembling enzyme and a second antibody with high proportions on an Au-Fe3O4 surface. According to the sandwich immunoassay principle, the concentration of Tg-IgM is determined by using an electrochemical signal generated by catalysis of enzyme to a substrate. According to the electrochemical immunosensor, the specificity of immunoreaction is combined with the sensitivity of electrochemical detection; the transmission of electronics is promoted by using the graphene, the polythionine, the gold nanoparticles, Au-Fe3O4 and other material; and the sensitivity of the detection is improved. The electrochemical immunosensor has the advantages of simplicity and convenience for operation, favorable regeneration performance and detection cost reduction. The electrochemical immunosensor prepared on the basis can be also used for detecting other immunological markers and has favorable application prospect in medical diagnosis.

The invention relates to a preparation method and an application of a gynecological tumor marker immune sensor constructed with ordered mesoporous carbon. The invention belongs to the technical field of novel nano-grade functional material and biosensor. The preparation method is characterized by: (1) the preparation of an ordered mesoporous carbon nano-grade material; (2) the preparation of an ordered mesoporous carbon-toluidin blue composite material; and (3) the preparation of the gynecological tumor marker immune sensor. The gynecological tumor marker immune sensor provided by the invention has the advantages of fast identification, high sensitivity, low detection limit, low cost, and convenient operation. With the gynecological tumor marker immune sensor, high-sensitivity, specific, rapid, and accurate detections of various gynecological tumor markers can be realized. Sample determination can be finished in 3min, such that batched sample determination can be realized within a short period of time.

The invention relates to a micro-array biochip, and a preparation method and an application of the micro-array biochip. The micro-array biochip comprises a glass chip substrate and a modification layer. The modification layer comprises a chromium layer, a silver and / or gold layer, a self-assembly monomolecular layer, and a photo-cross-linking agent and high-molecular layer, which are overlaid in sequential on the surface of the glass chip substrate. The invention also provides a protein micro-array, a method for preparing the protein micro-array, and an application of the protein micro-array to detect micromolecule medicine. According to the invention, three-dimensional high-molecular surface modification of the micro-array biochip is completed by the method, and the fixation amount of protein on the surface of the micro-array biochip can be effectively improved, so that micromolecule, hard to detect in the prior art, can be detected without marking, in accuracy, and in high flux. The micro-array biochip is of great significance to the field, such as molecular biology study and medicinal development.

The invention relates to a selective absorber of low-density lipoprotein cholesterol, which comprises carrier and functional group. Wherein, the carrier is macromolecule whose surface has free function group hydroxyl and / or amido; the functional group comprises disebrin and its derivate, or one of ateroid; the functional group via coupler is fixed on the carrier. The invention also provides relative production that selecting the macromolecule with surface function group as carrier, uses conjugated linkage to fix functional group, keeps active group carboxyl and sulfonic group, to keep the function group of specific adsorption LDL and, and hold the material from dropping in application, to improve safety. The invention has low cost and simple process.

The invention discloses a self-assembled modified nano gold spherical array bio-sensitive film and a preparation method thereof. A nano gold particle spherical structure of which the surface is modified with a binary mixed self-assembled film is arranged on a self-assembled unimolecule layer modified gold electrode in an array form to fix biological macromolecules so as to form a bio-sensitive film. The bio-sensitive film is prepared by the following steps of: A) mixing two thiol solvents with different carbon chain lengths of which a functional group is carboxyl at one end according to an equal proportion so as to prepare thiol mixed solution; B) soaking a working electrode in the mixed solution of the step A; C) adding the same amount of or excessive thiol mixed solution into nano gold sol solution according to a molar ratio, and adding a surfactant to prepare mixed self-assembled film saturation modified nano gold sol; D) soaking the working electrode of the step B in the nano gold sol of the step C; and E) dripping solution of biological macromolecules on the surface of the working electrode of the step D, and standing to form the bio-sensitive film.

The invention discloses a surface modification method of a biomaterial magnesium alloy. The method comprises, firstly, performing surface chemical treatment on the surface magnesium alloy; secondly, self-assembling and immobilizing amino silane molecules; lastly, sequentially immobilizing polyethylene glycol, fibronectin and heparin on the surface to structure a multifunctional bioactive surface and meanwhile to improve the physiological corrosion-resistant performance of the magnesium alloy. Through surface modification of the biomaterial magnesium alloy, the method can improve the physiological corrosion-resistant performance of the magnesium alloy and endow the material with good blood compatibility and endothelial cell growth promoting property, thereby laying a good foundation for application of the biomaterial magnesium alloy to the fields of intravascular implantation of materials or devices such as intravascular stents.

The invention relates to an electrochemical immune sensor for phosphating protein. In the electrochemical immune sensor, a magnetic nanometer-antibody composition MPs-p53<15>Ab1 is used as an adsorbent to separate the phosphating protein, a carbon nanometer sphere CNS is used as a carrier, lead phosphate-apoferritin (LPA) and a phosphating protein antibody p5315Ab2 are modified on the surface of the CNS, and thus, LPA-p53<15>Ab2-CNS nanometer complexing agent is prepared for detecting signal amplification. According to the sandwich immunoassay principle, the content of the phosphating protein to be detected is in proportion to the captured LPA-p5315Ab2-CNS, and the content of lead ions encapsulated in LPA is detected according to a dissolving volt-ampere method to measure the concentration of the phosphating protein in a sample. The method has the advantages of simplicity and convenience for operation and simple and efficient separation, and the advantage that an integral immunologic reaction is finished in an epoxy (EP) tube; a large number of LPA signal molecules are introduced by taking the CAN as the carrier, and each LPA molecule contains a large number of Pb<2+>, so the sensitivity of detection is improved; and the dissolved Pb<2+> is used as a detection signal without adding an enzyme substrate, so the electrochemical immune sensor is a reagent-free sensor. In the electrochemical immune sensor for the phosphating protein, the linear concentration range of the phosphating protein phosphor-p53<15> is from 0.02 to 20 ng mL<-1>, and the detection limit is 0.01 ng mL<-1>.

The invention belongs to the technical field of a micro-fluidic chip, and particularly relates to a paper element micro-fluidic chip proteolysis reactor and a preparation method thereof. The preparation method comprises the following steps: cutting a piece of filter paper into strips or a needed shape, wetting the filter paper, then adhering the filter paper onto a thermoplastic transparent plastic sheet which is cut into the size of a chip, airing the filter paper, covering the filter paper with a piece of plastic sheet in the same size with solution connection holes, and clamping the filter paper between two glass sheets, and then pressing by utilizing a clamp; and heating for 5 to 15 minutes in a drying oven with temperature of 110 to 180 DEG C, encapsulating a paper element between two plastic sheets, and obtaining a paper element micro-fluidic chip with a paper fiber filling channel, wherein the width of the channel with the paper element is 0.5mm to 3mm; and then filling the channel with a periodic acid solution, oxidizing cellulose in the paper element into aldehyde groups, cleaning, and injecting such a protease solution as trypsin solution so as to fix the protease by covalent bonds to obtain a proteolysis micro-fluidic chip. The reactor is simple and convenient to process, short in enzymolysis time and small in sample dosage.

The invention provides a preparation method of an electrochemical sensor for detecting 2,4,6-trichlorophenol. The preparation method comprises steps as follows: Cu-MOF-74 crystals are prepared from 2,5-dihydroxyterephthalic acid and copper nitrate trihydrate with a hydrothermal synthesis method; hemin is dissolved in a methanol aqueous solution, and a hemin stock solution is prepared; the Cu-MOF-74 crystals and the hemin stock solution are mixed, hemin and Cu-MOF-74 are subjected to coordination, hemin is immobilized on surface of Cu-MOF-74 and in pore channels, and a hemin / Cu-MOF-74 compositematerial is prepared; the hemin / Cu-MOF-74 composite material is dispersed in DMF, an electrode modification solution is prepared and dropwise applied to the surface of a polished glassy carbon electrode, then the glassy carbon electrode is put in a dryer and dried at room temperature, and the electrochemical sensor for detecting 2,4,6-trichlorophenol is obtained. The prepared hemin / Cu-MOF-74 electrochemical sensor has lower detection limit, can flexibly detect and measure 2,4,6-trichlorophenol in an actual sample, and is simple in technical operation, high in sensitivity and low in cost.

The invention discloses a preparation method for an immunosensor based on gamma-polyglutamic acid grafted dopamine and chitosan complex micelles. The preparation method comprises the following steps: preparing polymeric micelles; preparing polymeric micelle modified electrodes; preparing the immunosensor. According to the preparation method, the prepared polymeric micelles are very good in biocompatibility, the application of the polymeric micelles to the immunosensor can effectively increase the immobilization amount of antibodies and well keep the activity of biomacromolecules, and the prepared immunosensor has the advantages of high specificity, good stability, wide detection range and the like; the combination of a macromolecular self-assembly technology and an electrochemical sensor can be widely applied to immunoassay and extendedly applied to the fields of food safety, biological medicines, environmental protection monitoring and the like.

The invention discloses a fly-ash-based carbon dioxide curing agent and a preparation method thereof. The fly-ash-based carbon dioxide curing agent comprises fly ash, slaked lime and aluminum oxide, wherein the weight ratio of fly ash to slaked lime to aluminum oxide is (3-4.5):(1.5-3):2. When the ratio of fly ash to slaked lime is 1:1, the carbon fixation amount is highest, and due to doping of the slaked lime, the leaching toxicity of fly ash after carbonation is reduced, and the fly-ash-based carbon dioxide curing agent is mainly used for leaching of heavy metals;fly ash is a coal burning product, is one of main bulk wastes in China at present, greenhouse-gas carbon dioxide is sealed up for storage by adopting bulk-solid-waste fly ash and the purpose of using wastes to treat wastes is achieved.

The invention discloses a preparation method of an immunosensor based on chitosan-gold hybrid particles. The preparation method comprises the following steps: catechol group-modified chitosan synthesis, preparation of modified chitosan-gold hybrid nanoparticles, and construction of the immunosensor. The hybrid nanoparticles have good biological compatibility, adhesiveness and conductivity, an application of the polymer-inorganic conductive hybrid nanoparticles in the immunosensor can effectively increase a fixed amount of an antibody and can better keep the activity of biomacromolecules, adhesive force and conductivity of a sensing coating layer can be effectively enhanced, so that the constructed immunosensor has the advantages of high specificity, good long-term stability, wide detectionscope, and low detection lower limit; a nanocomposite material and an electrochemical sensor are combined, and the immunosensor can be widely used for immunization analysis and development in the fields of food safety, biological medicine and environmental protection monitoring.

The invention discloses a microorganism sample rapid detection method and a detection device thereof, which is realized by a mesoporous biochip. The detection method comprises the following steps: firstly fixing antibodies on micropore tunnel surfaces of a mesoporous biochip, allowing a test sample to flow through the chip micropore tunnels with the antibodies fixed so as to realize separation and enrichment of pathogenic microorganisms or other biological substances by the chip, then injecting a solution containing a fluorescence-labeled or an enzyme-labeled antibody into the chip micropore tunnel to form a sandwich-type antibody-antigen-labeled antibody immune complex, and detecting the pathogenic microorganisms or other biological substances by detecting the fluorescence intensity of the fluorescence-labeled antibody or the light absorption or chemiluminiscence intensity of the product of the enzymic catalytic reaction so as to realize the detection of the microorganisms to be detected. The mesoporous biochip has a great number of micropore tunnels, which greatly increases the reaction area, and thus when used for microorganism sample detection, the detection method and the detection device of the invention have the characteristics of high detection sensitivity and high detection speed.

The invention relates to the field of medical devices, in particular to a preparation method of a surface-heparinized anticoagulation medical device based on ionic bond-covalent bond synergistic action. The method comprises the following steps: firstly, fixing heparin on the surface of a medical device by utilizing a hydrophilic polymer modified by low-molecular-weight hyperbranched polyethyleneimine as a side chain in an ionic bond combination manner; and furthermore, grafting a stable heparin coating through covalent bonds, so as to obtain the anticoagulant biomedical device with high anticoagulant activity and high anticoagulant stability. The device has a wide application value in the field of biomedical treatment. The method is simple in preparation process, high in controllability and low in material price, does not involve toxic reagents in the experiment process, has universality for various medical device structures, and is suitable for industrial production.

The invention provides a method for improving immobilization of chloridion in hardened cement paste, and belongs to the technical field of building materials. According to the method provided by the invention, 3-5% by mass of glass fiber and 0.025-0.1% by mass of triethanolamine are put into coal ash doped cement paste, so that the immobilization property of the chloridion in the hardened cement paste can be remarkably improved. The hardened cement paste prepared by using the method can be applied to sea sand or marine concrete, is capable of effectively inhibiting damage of chloridion, and has a function of alleviating rusting of concrete reinforcement.

The invention discloses a directional covalent immobilization method of biotin-protein ligases (BirA) on a magnetic microsphere surface. The method comprises the following steps: firstly, using a genetic engineering technology to express a biotin-protein ligase recombinant protein of which the N-terminal has an MLAQGS (methionine-leucine-alanine-glutamine-glycine-serine) sequence and a polyhistidine sequence; then utilizing the catalysis of transglutaminase, making -NH2 of glutamine in the biotin-protein ligase recombinant protein and -NH2 on the amino-modified magnetic microsphere surface form covalent connection, thus directionally immobilizing the biotin-protein ligases on the magnetic microsphere carrier surface by a manner of covalent binding. According to the invention, the new method for immobilizing BirA is established, so that the enzymatic catalysis activity of BirA can be fully kept, and can be repeatedly used to improve the use efficiency of BirA and lower the use cost.

The ion exchange effect between a cation-containing polymeric material Nafion film modified glassy carbon electrode and cations in ruthenium terpyridine is used to immobilize ruthenium terpyridine to the surface of the electrode and dope the Nafion film with multi-walled carbon nano-tubes and zinc oxide nano-particles. The promotion effect of amido groups on the electrochemiluminescence of ruthenium terpyridine realizes histamine detection. The method using the MWCNTs / ZnO / Nafion compound film modified electrode to immobilize the ruthenium terpyridine has the advantages of reduction of the use amount of the ruthenium terpyridine, reduction of the cost of experiments, reduction of harms to environment and improvement of the stability and the repeated utilization rate of the experiments. The electrochemiluminescence sensing method used for histamine detection also has the advantages of wide application range, low detection limit and good stability and repeatability, and can be applied to detection of histamine in a practical sample.

The invention discloses organo-mineral biochar complex formula fertilizer and a preparation method thereof. The preparation method of the organo-mineral biochar complex formula fertilizer comprises the following steps: (1) a premix is obtained by mixing organic matter with biochar; (2) the premix obtained in the step (1) is subjected to composting fermentation; (3) the organo-mineral biochar complex formula fertilizer is obtained by adding chemical fertilizer to a fermentation product obtained in the step (2). According to the complex formula fertilizer, plant-sourced organic matter and animal-sourced organic matter are subjected to mixed fermentation, raw materials are subjected to biosafety disposal, the biochar is formed and plays an adsorption role, and clean organic fertilizer is produced; a balanced and reasonable NPK formula is realized according to the organic matter with difference sources, and maximization of the fertilizer efficiency is realized.

The invention provides a cheap inorganic porous adsorption carrier capable of being used for cell immobilization. Clay is fired to prepare the carrier; the carrier has the characteristics of porosity, high mechanical strength, stable properties, low price, large cell immobilization quantity and the like, is simple in cell immobilization operation, is non-toxic for microbial cells and can be reused; and the carrier can be used for industrial-scale cell immobilization. The carrier is used to immobilize nitrobacteria so as to prepare immobilized nitrobacteria used for converting ammonium in a water body to nitrate; and the conversion speed of the obtained immobilized nitrobacteria to ammonium is high, and the efficiency is high. The method for converting ammonium with the immobilized nitrobacteria comprises the following steps: the immobilized nitrobacteria are placed in a netted container, the netted container is placed in an ammonium sulfate solution or ammonium-containing water body, and after a certain time period, the immobilized nitrobacteria can convert ammonium to nitric nitrogen. The immobilized nitrobacteria in the netted container can be reused. After the immobilized nitrobacteria are respectively placed in a 5g / L ammonium sulfate solution and a 20g / L ammonium sulfate solution for 4h, the average conversion rates of ammonium nitrogen are respectively 79.7%-83.6% and 86.5%-86.7%. After being stored for half a year at 4 DEG C, the nitrification ability of the immobilized nitrobacteria is not reduced.

The invention belongs to the technical field of ecology agriculture, and concretely relates to a preparation capable of enhancing absorption of carbon dioxide by plant. The invention discloses a formula of a preparation capable of enhancing absorption of carbon dioxide by plant and a production technology, the preparation comprises the following components by weight: 20-50% of carbon dioxide absorbent, 10-20% of photosynthetic enzyme, 10-20% of photosynthetic enzyme stimulating hormone, 1-20% of auxiliary agent and 0-55% of water. The preparation can be a liquid preparation or a solid preparation. The preparation capable of enhancing absorption of carbon dioxide by plant can reduce the usage of fertilizer and chemical pesticide, can effectively promote the yield of the crops, can improve the ecology environment and soil structure, and can increase the crops quality and reduce the production cost.

The invention discloses preparation and an application of an electrochemical luminescence sensor for detecting procalcitonin based on energy transfer between g-C3N4 and CuO, and belongs to the technical field of construction of novel sensors. Based on the good specificity between antigen and antibody, the sensor utilizes Au and carbon nanotube modified g-C3N4 composite material g-C3N4-CNT@Au as asubstrate luminescent material and polydopamine coated CuO as a quenching agent, and the electrochemical luminescence sensor is constructed through layer-by-layer self-assembly. The electrochemical luminescence sensor constructed by the invention has a wider detection range, higher sensitivity and lower detection limit, and has important significance for detection of procalcitonin.

The invention relates to an immobilized enzyme and a preparation method thereof. Chitin nanofibers are used as a carrier, wherein the enzyme is fixed to the carrier in a covalent connection manner, and the hydroxyl of the chitin nanofibers is oxidized into a carboxylic acid group for covalent connection of the enzyme and the chitin nanofibers. The hydroxyl of the chitin nanofibers is oxidized into the carboxylic acid group, so that the enzyme can be in covalent connection through the carboxylic acid group to be fixed to the carrier, and a cross-linking agent is used as assistance for further fixing the enzyme; and through cooperation of the methods, the fixing quantity of the enzyme is further increased, the stability of the enzyme is improved, and the catalyzing efficiency of the enzyme is further improved.

The invention discloses a method of removing an antibiotic from a water body with hollow mesoporous carbon immobilized laccase. The method comprises the following steps of: adding hollow mesoporous carbon immobilized laccase into a citric acid-sodium citrate buffer solution containing the antibiotic for oscillating reaction, allowing the antibiotic to enter a hollow inner cavity through mesoporouschannels on a hollow mesoporous carbon shell and be absorbed and degraded by laccase immobilized by the hollow inner cavity, and accomplishing the removal of the antibiotic. The method of removing the antibiotic from the water body with hollow mesoporous carbon immobilized laccase has the advantages that the method is simple, effective, easy to implement and high in safety and causes no secondarypollution.

A process for producing a micro fluid device in which a porous resin layer capable of fixing a most appropriately large amount of proteins, such as enzymes and antigens, catalysts, etc. on the inside surface of minute channels of the micro fluid device without clogging of the channels is provided with a uniform thickness on the surface of the channels. Providing of a porous resin layer with a uniform thickness on the surface of the channels can be easily accomplished through a process comprising forming in advance on a support a porous resin layer having a multiplicity of pores on its surface, applying an actinic energy radiation hardening composition onto the porous resin layer so as to form depressed portions having the porous resin layer at bottoms thereof, and thereafter securing a covering member to the depressed portions so as to provide channels.

The invention discloses an electrochemical immunosensor based on a DNA functionalized nano composite material. The electrochemical immunosensor is formed by the steps: a matrix electrode is loaded with a dendritic metal, then a mesoporous material loaded with an antibody label is added, next a DNA hybrid chain is introduced, and finally, an active substance is added. Preparation comprises the following steps: 1) preparing a matrix material; 2) preparing the mesoporous material loaded with the antibody label; and (3) constructing the electrochemical immunosensor. The invention discloses an application of the electrochemical immunosensor based on the DNA functionalized nano composite material in detection of toxins. Based on a strategy of multiple signal amplification, the electrochemical immunosensor with high sensitivity and low detection limit is constructed. With combination of the high amplification ability of a DNA amplification technology, high sensitivity of electrochemical detection, signal amplification of nano material surface effects and other strategies, the rapid immunoassay is established, and trace toxins are detected.

The invention relates to a micro-nano carbon long-lasting environment-friendly mixed magnetic fertilizer which comprises the following components in percentage by weight: 0.03-0.05% of micro-nano carbon, 1-1.5% of magnetic iron-ore slag powder, 11-15% of humic acid, 2.8-4.2% of ammonium bicarbonate, 0.1-0.3% of rare earth, 0.01-0.07% of catechol, 1.5-1.7% of nanometer silicon powder and the balance of a chemical fertilizer, wherein the chemical fertilizer comprises nitrogen, phosphorus, potassium, calcium, magnesium, zinc, manganese, iron, copper and boron. According to crops, 20-50kg of the mixed magnetic fertilizer is applied to each mu of farmlands. The mixed magnetic fertilizer is tested in cereal crops such as corn, wheat, rice and various coarse cereals and economic crops such as cotton, watermelons, sweet melons and vegetables, the yield increase is high, the per unit yield of field crops is increased by 5-15%, the per unit yield of vegetable crops is increased by 20-40%, and fertilizers can be saved by 20-30%.

The invention discloses a method for improving concrete cement paste chlorine ion combination long residual action, and belongs to the technical field of building materials. The method comprises the following steps: adding ferric hydroxide and silicon ash to cement paste, and doping triethanolamine or triisopropanolamine, wherein a total mass of a cement drier, the ferric hydroxide and the siliconash is 100%, wherein the ferric hydroxide is 5%-10%, the silicon ash is 2%-5%, the triethanolamine accounts for 0.01%-0.1% of a total mass of the above three parties, and the triisopropanolamine accounts for 0.0025%-0.1% of the total mass of the above three parties. The method is capable of improving chlorine ion fixation capacity of cement concrete, widening an application of the triethanolamineand the triisopropanolamine in the cement concrete, and relieving a corrosion problem of a reinforcing steel bar because of the action of chlorine ions in seawater.

The invention relates to the field of biological detection, in particular to a peanut allergen specific IgE quantitative detection reagent, a kit and application thereof. The kit is based on a magnetic particle chemiluminescence method, used allergens are subjected to biotinylation modification based on hydrophilic polymers, and the kit is high in sensitivity, good in specificity, short in reaction time and high in automation degree.