The invention provides a novel electrochemical sensor for detecting CREG1 based on a surface
molecular imprinting technology and a preparation method of the novel electrochemical sensor. Comprising the following steps: step 1, preparing a
reagent solution required in a manufacturing process; step 2,
polishing the
glassy carbon electrode (GCE); 3, AuNPs / GCE is prepared; step 4, preparing CREG1 / GA (at) CS / AuNPs / GCE; step 5, preparing MIP / GA (at) CS / AuNPs / GCE; and step 6, scanning in the
redox probe solution by adopting a
differential pulse voltammetry (DPV), and determining the content of the CREG1. According to the invention, CREG1
protein is selected as a detection template, and a
molecularly imprinted polymer is combined with gold nanoparticles through a surface
polymerization method to prepare a corresponding
western blot material. Gold nanoparticles are used as a matrix, CREG1 is used as a template, GA (at) CS is used as a
protein fixing agent, and
polyphenol is used as an imprinted
polymer to prepare a surface imprinted material, and the surface imprinted material is measured by measuring the change of sensor current before and after the combination of a molecularly imprinted membrane (MIP) and CREG1. According to the present invention, the surface
molecular imprinting technology and the electrochemical sensing technology are combined to construct the high-sensitivity and high-specificity CREG1 sensor, and the high-sensitivity and high-specificity CREG1 sensor is the novel surface
molecular imprinting electrochemical sensor with good
biocompatibility, can simply, conveniently and rapidly recognize the CREG1
protein at a low cost, and has characteristics of high sensitivity and high sensitivity. In addition, the method can also be used as a universal sensing platform to be suitable for other proteins.