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Preparation method of multi-growth-factor slow release coating of titanium and titanium alloy surface

A technology of growth factors and titanium alloys, which is applied in the fields of surface coating liquid devices, coatings, medical science, etc., can solve the problems of unstable coating structure, slow release of growth factors, etc., and achieve convenient slow release time and Sequence, high immobilization amount, effect of improving stability

Inactive Publication Date: 2013-03-06
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it can maintain the activity of growth factors to the greatest extent and control the release sequence of different growth factors; however, the amount of growth factors that can be loaded in polymer materials is relatively small, and the interpenetration of polymer materials between layers makes the self-assembled coating The layer structure is unstable, and it is difficult to stably regulate the sustained release of various growth factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for preparing a slow-release coating of multiple growth factors on a titanium surface, the specific steps are as follows:

[0029] A. Loading of growth factors:

[0030] A1. Configure a mixed solution of chitosan (polycation) and dopamine at a concentration of 1 mg / mL;

[0031] A2. Dissolve the four growth factors of BMP-2, VEGF, FGF and TGF-β in a heparin (polyanion) solution with a concentration of 1 mg / mL to obtain growth factors containing BMP-2, VEGF, VEGF and TGF-β respectively The four groups of heparin solutions of factor, the concentration of growth factor in each group of heparin solution is 5μg / mL;

[0032] A3. Mix the four groups of polyanion solutions of step A2 with the mixed solution of step A1 in a volume ratio of 4:1, stir, centrifuge, and freeze-dry to prepare four groups of negatively charged nanoparticles; four groups of negative The charged nanoparticles carry the growth factors BMP-2, VEGF, VEGF and TGF-β, respectively.

[0033] B. Graft a negativ...

Embodiment 2

[0040] A method for preparing a slow-release coating of multiple growth factors on the surface of a titanium alloy, the specific steps are as follows:

[0041] A. Loading of growth factors:

[0042] A1. Configure a mixed solution of polylysine (polycation) and dopamine at a concentration of 2 mg / mL;

[0043] A2. Dissolve BMP-2 and TGF-β growth factors in a sodium alginate (polyanion) solution with a concentration of 2mg / mL to obtain two groups of alginic acid containing BMP-2 and TGF-β growth factors. Sodium solution, the concentration of growth factor in each group of sodium alginate solution is 10μg / mL;

[0044] A3. The two groups of sodium alginate (polyanion) solutions from step A2 are mixed with the mixed solution of step A1 in a volume ratio of 5:1, and stirred, centrifuged, and freeze-dried to prepare BMP-2 containing growth factors. Two groups of negatively charged nanoparticles with TGF-β;

[0045] B. Graft a negatively charged functional group on the surface of the titanium ...

Embodiment 3

[0052] A method for preparing a slow-release coating of multiple growth factors on a titanium surface, the specific steps are as follows:

[0053] A. Loading of growth factors:

[0054] A1. Configure a mixed solution of chitosan (polycation) and dopamine at a concentration of 0.5 mg / mL;

[0055] A2. Dissolve the two growth factors, BMP-2 and FGF, in a hyaluronic acid solution with a concentration of 0.5 mg / mL to obtain two groups of hyaluronic acid solutions containing BMP-2 and FGF growth factors. Each group of hyaluronic acid The concentration of growth factor in acid (polyanion) solution is 0.5μg / mL;

[0056] A3. Mix the two groups of hyaluronic acid solutions from step A2 with the mixed solution of step A1 in a volume ratio of 3:1, stir, centrifuge, and freeze-dry to prepare two groups of negatively charged nanoparticles, one of which The nanoparticles contained the growth factor BMP-2, and the other group contained the growth factor FGF.

[0057] B. Graft a negatively charged fun...

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PUM

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Abstract

The invention discloses a preparation method of a multi-growth-factor slow release coating of a titanium and titanium alloy surface. According to the method, a plurality of layers of coatings containing nanoparticles of growth factors are prepared on the titanium surface by taking the nanoparticles as a growth factor carrier and charged high molecular thin films as a medium. The nanoparticles included in the coating are prepared by mixing polycation solution containing dopamine of certain concentration with polyanion solution containing the growth factors. The nanoparticles have good dispersibility, can protect the activity of the growth factors and are easy to fix on the surface of a material. By controlling the electric property of the nanoparticles and the high molecular thin films, nanoparticles carrying with different factors are embedded between the high molecular thin films. By adjusting the loading quantity and the loading sequence of different nanoparticles and the thickness of the coatings, effective control over release speed, release quantity and release sequence of the various growth factors can be realized.

Description

Technical field [0001] The invention relates to a method for preparing multiple growth factor slow-release coatings on the surface of medical titanium and titanium alloy. Background technique [0002] Titanium and titanium alloys have been widely used in plastic surgery and dentistry due to their good biocompatibility and excellent mechanical properties. However, titanium and titanium alloys are biologically inert materials. After being implanted in the human body, the regeneration of new bone on the surface is slow, and the bond between the new bone and the titanium surface is weak. In order to improve the surface activity and osseointegration ability of titanium implants, it is often necessary to perform functional treatment on the titanium surface. Commonly used methods to improve biological activity on titanium surface include alkaline heat treatment, anodization, hydroxyapatite coating, and loading of biomolecules and specific growth factors that promote cell adhesion and g...

Claims

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Application Information

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IPC IPC(8): B05D7/14B05D7/24A61L27/40A61L27/34A61L27/54
Inventor 鲁雄王振铭翁杰冯波屈树新汪建新
Owner SOUTHWEST JIAOTONG UNIV
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