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Electrochemical immunosensor based on DNA functionalized nano composite material

A technology of nanocomposite materials and immunosensors, applied in the direction of material electrochemical variables, etc., can solve problems such as differences in catalytic activity, and achieve high sensitivity and low detection limit effects

Active Publication Date: 2017-04-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although G-quadruplex-Hemin DNase has the advantages of easy preparation and low price, compared with natural horseradish peroxidase, there is still a big difference in its catalytic activity

Method used

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  • Electrochemical immunosensor based on DNA functionalized nano composite material
  • Electrochemical immunosensor based on DNA functionalized nano composite material
  • Electrochemical immunosensor based on DNA functionalized nano composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0060] 1) Synthesis of silica nanospheres

[0061] Add 0.8mL of 28% ammonia water to the mixture containing 36mL of ethanol and 5mL of distilled water, stir gently at room temperature for 10 minutes, then add 1mL of TEOS to the above mixture, stir magnetically at room temperature for 3 hours, and finally centrifuge the product. After washing three times with distilled water and absolute ethanol, the product was added to 6mL of absolute ethanol to disperse for later use.

[0062] 2) Synthesis of SiO 2 @MSN core-shell nanomaterials

[0063] Add 0.5g CTAB to 75mL distilled water, ultrasonically treat until CTAB is completely dissolved, add 0.8mL ammonia water, then add dropwise 3mL ethanol solution of silica nanospheres prepared above, ultrasonically disperse for 30 minutes, add 20mL n-hexane, and After magnetically stirring at 500 rpm at 30°C for 12 minutes, adjust the rotating speed to 170rpm and slowly add 2.5mL TEOS dropwise (the dropwise addition was completed in 20 minute...

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Abstract

The invention discloses an electrochemical immunosensor based on a DNA functionalized nano composite material. The electrochemical immunosensor is formed by the steps: a matrix electrode is loaded with a dendritic metal, then a mesoporous material loaded with an antibody label is added, next a DNA hybrid chain is introduced, and finally, an active substance is added. Preparation comprises the following steps: 1) preparing a matrix material; 2) preparing the mesoporous material loaded with the antibody label; and (3) constructing the electrochemical immunosensor. The invention discloses an application of the electrochemical immunosensor based on the DNA functionalized nano composite material in detection of toxins. Based on a strategy of multiple signal amplification, the electrochemical immunosensor with high sensitivity and low detection limit is constructed. With combination of the high amplification ability of a DNA amplification technology, high sensitivity of electrochemical detection, signal amplification of nano material surface effects and other strategies, the rapid immunoassay is established, and trace toxins are detected.

Description

technical field [0001] The invention relates to an electrochemical immunosensor based on DNA functionalized nanocomposite material. Background technique [0002] A biosensor is a device or device that can sense a specified measurement and convert it into a usable output signal according to a certain degree. It is a type of sensor composed of immobilized biological materials as sensitive elements and combined with appropriate conversion elements. According to the molecular recognition elements in biosensors, that is, sensitive elements, they can be divided into five categories: enzyme sensors, microbial sensors, organelle sensors, tissue sensors and immune sensors. The sensitive materials used in these five types of sensors are enzymes, individual microorganisms, organelles, slices of animal and plant tissues, and immune substances. Among them, immunosensors are widely used in environmental monitoring, clinical diagnosis, food safety and other fields due to their advantages ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 刘英菊甘翠芬何祖宇魏婕敖日其冷
Owner SOUTH CHINA AGRI UNIV
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