35 results about "Rapid immunoassay" patented technology

The invention discloses a micro-fluidic device for micro-magnetic field control and a manufacturing method thereof. The device consists of a micro-fluidic fluid layer chip, a substrate provided with soft magnetic material micro-patterns, and a magnetic source body. The substrate provided with the soft magnetic material micro-patterns is a transparent conducting substrate, wherein the micro-patterns are arranged and designed corresponding to needed magnetic beads, and a layer of a polymeric material is covered on the micro-patterns to realize a connection in a permanent irreversible bonding mode with the micro-fluidic fluid layer chip. Under the action of an external magnetic field, nickel micro-patterns are magnetized and generate a strong local magnetic field gradient to realize the magnetic field control in a micron range. The device can capture magnetic substances at a high flow speed, and can release the magnetic substances at a low flow speed after the magnetic source body is removed. The device can be used in the fields of organism-targeted material detection, gene analysis, quick immunoassay, cell screening and the like.

The invention discloses a fluorescent microsphere immunochromatography detection card for detecting enrofloxacin and a preparation method thereof. The detection card sequentially comprises filter paper, a sample pad, a glass fiber film, a nitrocellulose film and absorbent paper, wherein a fluorescent microsphere-labeled antibody is sprayed on the glass fiber film; a detection area and a quality control area are fixed on the nitrocellulose film; a conjugate of enrofloxacin and carrier protein is sprayed in the detection area; and an anti-mouse antibody is sprayed in the quality control area. By taking core-shell bistructure luminous nano particles complexed by silica and fluorescent substances as labels and adopting immunochromatography in a competition blocking mode, the invention realizes rapid immunoassay of the enrofloxacin. In the detection process, an optimized excitation light source of the fluorescent microsphere is adopted for excitation, the emitted fluorescence passes through a light filter device, and that whether a detection line is provided with fluorescent substances is observed by naked eyes. The invention has the characteristics that: the detection card has high sensitivity and is rapid in detection, convenient to operate, and economic and practical.

The present invention relates to a method of diagnosing and monitoring various distinct presentations of tuberculosis: active tuberculosis disease, latent tuberculosis infection and recent tuberculosis infection. The rapid immune assay is based on the evaluation of the frequency of Interferon (IFN) gamma-producing antigen-specific T lymphocytes responding to selected peptide sequences from Mycobacterium tuberculosis, selected for their immunogenicity. The invention concerns also immunogenic and vaccine compositions based on these specific peptide sequences.

The disclosed magnetic immunoassay device, which performs magnetic immunoassays using antigen-antibody reactions, can perform speedy immunoassays without bound/free separation in the test samples. The device is also practical, being capable of stable magnetism measurement without magnetic shielding. The disclosed magnetic immunoassay device is provided with: an excitation coil that uses an AC magnetic field to magnetize a test sample containing a magnetic marker; a magnetism sensor that measures magnetism in the test sample and outputs a magnetism signal; and a displacement sensor for detecting changes in the distance between the test sample and the magnetism sensor. By optimally setting the bandwidth of a lock-in amplifier, which detects changes in the phase of the magnetism signal outputted by the magnetism sensor, and the rotational speed produced by a drive system, which moves the test sample at low speeds, the impact of environment magnetic noise is reduced, and correcting the magnetism signal using distance information obtained from the displacement sensor allows stable magnetism measurement.

The invention discloses a test strip for detecting methylamphetamine and a preparation method and an application method of the test strip. The test strip comprises a sample absorption pad, a conjugaterelease pad, a reaction membrane, a water absorption pad and a substrate in sequence, wherein an antibody which is marked by time resolution fluorescent microspheres is sprayed to the conjugate release pad; a detection line of hapten-carrier protein conjugate and a quality control line of a sheep anti-rat secondary antibody are coated on the reaction membrane. By adopting the test strip, the antibody is marked by the time resolution fluorescent microspheres, immunochromatography assay is adopted, and rapid immunoassay of methylamphetamine can be achieved. The invention further provides a method for detecting methylamphetamine in a sample by using the test strip. The test strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate and economic and practical, and rapid detection and on-site detection on large-scale methylamphetamine samples can be achieved.

The invention discloses a reagent strip carrier device used for a rapid immunoassay analyzer. The reagent strip carrier device is disc-shaped and comprises multiple separable groove-shaped structures used for placing reagent strips. Besides, the invention also discloses a use method for the reagent strip carrier device. The reagent strip carrier device used for the rapid immunoassay analyzer is low in cost and convenient for use, and can flexibly select one or more of (high flux) reagent strips to analyze at one time.

The invention discloses a test paper strip for detecting morphine as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, a conjugaterelease pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by time resolved fluorescence microspheres; the reaction membrane is coated with a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the time resolved fluorescence marked antibody and an immunochromatography technique, rapid immunoassay of morphine can be achieved. The invention further provides a method for detecting morphine in a sample by using the test paper strip. The test paper strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of morphine samples can be achieved.

The invention relates to a triple test strip for detecting methamphetamine, morphine and ketamine as well as a preparation method and an application method of the triple test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody, a morphine antibody and a ketamine antibody marked by the time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine antigen, a detection line T2 of a morphine antigen, a detection line T3 of a ketamine antigen and a quality control line C of a goat anti-mouse secondary antibody or Biotin-BSA. According to the immunochromatographytechnology, the simultaneous and rapid immunoassay of three drugs including methamphetamine, morphine and ketamine is realized. The invention also provides a method for simultaneously detecting the methamphetamine, morphine and ketamine in a sample by using the above test strip. The triple test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

The invention discloses an electrochemical immunosensor based on a DNA functionalized nano composite material. The electrochemical immunosensor is formed by the steps: a matrix electrode is loaded with a dendritic metal, then a mesoporous material loaded with an antibody label is added, next a DNA hybrid chain is introduced, and finally, an active substance is added. Preparation comprises the following steps: 1) preparing a matrix material; 2) preparing the mesoporous material loaded with the antibody label; and (3) constructing the electrochemical immunosensor. The invention discloses an application of the electrochemical immunosensor based on the DNA functionalized nano composite material in detection of toxins. Based on a strategy of multiple signal amplification, the electrochemical immunosensor with high sensitivity and low detection limit is constructed. With combination of the high amplification ability of a DNA amplification technology, high sensitivity of electrochemical detection, signal amplification of nano material surface effects and other strategies, the rapid immunoassay is established, and trace toxins are detected.

The invention relates to a duplex test strip for detecting morphine and ketamine as well as a preparation method and an application method of the duplex test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a morphine antibody and a ketamine antibody marked by the time-resolved fluorescentmicrospheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a morphine hapten-carrier protein conjugate, a detection line T2 of a ketamine hapten-carrier protein conjugate and a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of morphine and ketamine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting morphine and ketamine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

The invention discloses a test strip for detecting 6-monoacetylmorphine as well as a preparation method and an application method of the test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, an antibody marked by the time-resolved fluorescent microspheres is sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T of a hapten-carrier protein conjugate and a quality control line C of a goat-anti-mouse secondary antibody. According to the invention, the antibody is labeled by time-resolved fluorescent microspheres, and an immunochromatography technology is adopted to realize the rapid immunoassay of 6-monoacetylmorphine. The invention also provides a method for detecting the 6-monoacetylmorphine in a sample by using the above test strip. The test strip and the method have the advantages of being high in sensitivity and rapid in detection,being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of 6-monoacetylmorphine samples.

The invention relates to a duplex test strip for detecting methamphetamine and ketamine as well as a preparation method and an application method of the duplex test strip. The test strip sequentiallycomprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody and a ketamine antibody marked by time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine antigen, a detection line T2 of a ketamine antigenand a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of methamphetamine and ketamine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting the methamphetamine and ketamine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

The invention discloses a test strip for detecting amphetamine and a preparation method and application of the test strip. The test strip comprises a sample absorption pad, a conjugate release pad, areaction membrane, a water absorption pad and a substrate in sequence, wherein an antibody which is marked by time resolution fluorescent microspheres is sprayed to the conjugate release pad; a detection line of hapten-carrier protein conjugate and a quality control line of a sheep anti-rat secondary antibody are coated on the reaction membrane. By adopting the test strip, the antibody is marked by the time resolution fluorescent microspheres, immunochromatography assay is adopted, and rapid immunoassay of amphetamine can be achieved. The invention further provides a method for detecting amphetamine in a sample by using the test strip. The test strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate and economic and practical, and rapid detection and on-site detection on large-scale amphetamine samples can be achieved.

The invention relates to a duplex test strip for detecting methamphetamine and morphine as well as a preparation method and an application method of the duplex test strip. The test strip sequentiallycomprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody and a morphine antibody which are marked by the time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine hapten-carrier protein conjugate, adetection line T2 of a morphine hapten-carrier protein conjugate and a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of methamphetamine and morphine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting the methamphetamine and morphine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being highin sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

An FITC test paper strip for detecting benzodiazepine, a preparing method thereof and an application method of the test paper strip are disclosed. The test paper strip includes a sample absorption pad, a conjugate releasing pad, a reaction film, a water absorbing pad and a bottom plate in order. The conjugate releasing pad is sprayed with an FITC labeled antibody. The reaction film is coated witha hapten-carrier protein conjugate detection line and a goat anti-mouse secondary antibody quality control line. The FITC labeled antibody is utilized to achieve benzodiazepine rapid immunoassay through immunochromatography. The invention also provides the application method for detecting benzodiazepine in a sample by utilizing the test paper strip. The test paper strip has advantages of high sensitivity, capability of accurate quantification, rapid detection, convenient operation, and capability of being practical and economical, and can achieve rapid detection and on-site detection for large-batch benzodiazepine samples.