35 results about "Rapid immunoassay" patented technology

The present invention relates to a method of diagnosing and monitoring various distinct presentations of tuberculosis: active tuberculosis disease, latent tuberculosis infection and recent tuberculosis infection. The rapid immune assay is based on the evaluation of the frequency of Interferon (IFN) gamma-producing antigen-specific T lymphocytes responding to selected peptide sequences from Mycobacterium tuberculosis, selected for their immunogenicity. The invention concerns also immunogenic and vaccine compositions based on these specific peptide sequences.

The invention provides a method for preparing a nitrocellulose chromatographic membrane for rapid immunoassay, comprising the following steps: (1) preparing a casting solution: dissolving nitrocellulose, cellulose acetate and a hydrophilic polymer in a mixing Solvent, then add surfactant, finally add non-solvent and stir evenly, after defoaming and filtering, cast film solution; (2) scraping film: apply cast film solution on the base material, the wet film thickness of coating is 50-1000μm; (3) Film formation: Evaporate most of the solvent of the wet film after coating in a vacuum chamber, and then undergo a high-temperature non-solvent-induced phase inversion to form a film. The preparation method of the nitrocellulose chromatographic membrane for rapid immunoassay provided by the invention solves the problem in the prior art that the nitrocellulose membrane used for detection is poor in hydrophilicity and uniformity, which affects the detection efficiency.

The present invention aims to provide a novel stool collection and rapid immunological detection device and its operation method which are low in cost, easy to manufacture, convenient for result observation, and can avoid contamination and infection in the detection process. The device includes a stool collection pipe (1), an outer cover (2) and a stool collection stick (3). The stool collection pipe (1) is composed of an upper pipe piece (11) and a lower pipe piece (12). The upper pipe piece ( The bottom of 11) is connected with the outer side wall of the lower pipe (12) and forms a groove (4); Describe the through hole (5) that the center of the mining stick (3) is connected. In the operation method of the device, except for collecting samples and placing test strips, there is no need to unscrew the outer cover (2) in other steps, the dilution or lysing of samples, and the detection are all in the stool collection tube (1) completed within. The invention is applied to the technical field of sample collection and detection devices.

The invention discloses an electrochemical immunosensor based on a DNA functionalized nano composite material. The electrochemical immunosensor is formed by the steps: a matrix electrode is loaded with a dendritic metal, then a mesoporous material loaded with an antibody label is added, next a DNA hybrid chain is introduced, and finally, an active substance is added. Preparation comprises the following steps: 1) preparing a matrix material; 2) preparing the mesoporous material loaded with the antibody label; and (3) constructing the electrochemical immunosensor. The invention discloses an application of the electrochemical immunosensor based on the DNA functionalized nano composite material in detection of toxins. Based on a strategy of multiple signal amplification, the electrochemical immunosensor with high sensitivity and low detection limit is constructed. With combination of the high amplification ability of a DNA amplification technology, high sensitivity of electrochemical detection, signal amplification of nano material surface effects and other strategies, the rapid immunoassay is established, and trace toxins are detected.

The present invention relate to the field of Chinese herbal medicine material field, in particular to a Chinese medicinal immunotherapeutic material and a method for detecting the dynamic mechanism oftumor, a method for extracting, choosing and detecting the related Chinese herbal medicine and specifically a method for extracting components from Chinese herbal medicine Chinese wolfberry or astragalus membranaceus, wherein the components can play fine, accurate and rapid immunoassay preventive effect. The method comprises the following steps: using fresh Chinese wolfberry or astragalus membranaceus as raw material, pulverizing, adding ethanol with 60-79% by mass and citric acid solution with 1-5% by mass according to the ratio of 60:1, adding positive charge resin with 10-20% by mass and soaking for 2-8 hours at 30-50 DEG C, pumping in methane microbubble for 10-30 min, so as to accurately and quickly play the effect of the immune function improvement and its tumor inspection and analysis mechanism prevention, and the method can obtain small and medium molecular polysaccharides with immune function quickly and accurately under limited production conditions, and the production conditions and equipment environment are simple and convenient.