Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
35 results about "Rapid immunoassay" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Microchannel Magneto-Immunoassay
A single microchannel is combined with external electromagnets for performing a fast immunoassay within a very small volume. Magnetic / luminescent nanoparticles serve as carriers for the antibodies and as internal luminescent standard. The immunoreaction is accelerated by applying alternating magnetic field by means of the external electromagnets, thus inducing oscillation of the particles and achieving better diffusion during the incubation steps. Using the electromagnets the particles are held into the channel for washing and luminescence detection steps. The luminescence of the particles serves as an internal calibration for the assay and helps to avoid experimental error from particle loss.
Owner:RGT UNIV OF CALIFORNIA
Micro-fluidic chip for micro-magnetic field control and manufacturing method thereof
InactiveCN101879467APermanent irreversible bondingPermanent bondingLaboratory glasswaresChemical/physical/physico-chemical processesMagnetic field gradientMagnetic bead
The invention discloses a micro-fluidic device for micro-magnetic field control and a manufacturing method thereof. The device consists of a micro-fluidic fluid layer chip, a substrate provided with soft magnetic material micro-patterns, and a magnetic source body. The substrate provided with the soft magnetic material micro-patterns is a transparent conducting substrate, wherein the micro-patterns are arranged and designed corresponding to needed magnetic beads, and a layer of a polymeric material is covered on the micro-patterns to realize a connection in a permanent irreversible bonding mode with the micro-fluidic fluid layer chip. Under the action of an external magnetic field, nickel micro-patterns are magnetized and generate a strong local magnetic field gradient to realize the magnetic field control in a micron range. The device can capture magnetic substances at a high flow speed, and can release the magnetic substances at a low flow speed after the magnetic source body is removed. The device can be used in the fields of organism-targeted material detection, gene analysis, quick immunoassay, cell screening and the like.
Owner:WUHAN UNIV
Fluorescent microsphere immunochromatography detection card for detecting enrofloxacin and preparation method thereof
InactiveCN102023210AQuick checkSimple and fast operationBiological testingLuminescent compositionsCarrier proteinSilicon dioxide
The invention discloses a fluorescent microsphere immunochromatography detection card for detecting enrofloxacin and a preparation method thereof. The detection card sequentially comprises filter paper, a sample pad, a glass fiber film, a nitrocellulose film and absorbent paper, wherein a fluorescent microsphere-labeled antibody is sprayed on the glass fiber film; a detection area and a quality control area are fixed on the nitrocellulose film; a conjugate of enrofloxacin and carrier protein is sprayed in the detection area; and an anti-mouse antibody is sprayed in the quality control area. By taking core-shell bistructure luminous nano particles complexed by silica and fluorescent substances as labels and adopting immunochromatography in a competition blocking mode, the invention realizes rapid immunoassay of the enrofloxacin. In the detection process, an optimized excitation light source of the fluorescent microsphere is adopted for excitation, the emitted fluorescence passes through a light filter device, and that whether a detection line is provided with fluorescent substances is observed by naked eyes. The invention has the characteristics that: the detection card has high sensitivity and is rapid in detection, convenient to operate, and economic and practical.
Owner:江西中德生物工程股份有限公司
Immune diagnostic assay to diagnose and monitor tuberculosis infection
InactiveUS7785607B2High detection sensitivityHigh sensitivityBacterial antigen ingredientsPeptide/protein ingredientsDiseaseT lymphocyte
The present invention relates to a method of diagnosing and monitoring various distinct presentations of tuberculosis: active tuberculosis disease, latent tuberculosis infection and recent tuberculosis infection. The rapid immune assay is based on the evaluation of the frequency of Interferon (IFN) gamma-producing antigen-specific T lymphocytes responding to selected peptide sequences from Mycobacterium tuberculosis, selected for their immunogenicity. The invention concerns also immunogenic and vaccine compositions based on these specific peptide sequences.
Owner:INST NAT PER LE MALATTIE INFETTIVE LAZZARO SPALLANZANI IRCCS
Immune diagnostic assay to diagnose and monitor tuberculosis infection
InactiveUS20070196878A1High detection sensitivityHigh sensitivityBacterial antigen ingredientsSugar derivativesT lymphocyteLatent tuberculosis
The present invention relates to a method of diagnosing and monitoring various distinct presentations of tuberculosis: active tuberculosis disease, latent tuberculosis infection and recent tuberculosis infection. The rapid immune assay is based on the evaluation of the frequency of Interferon (IFN) gamma-producing antigen-specific T lymphocytes responding to selected peptide sequences from Mycobacterium tuberculosis, selected for their immunogenicity. The invention concerns also immunogenic and vaccine compositions based on these specific peptide sequences.
Owner:INST NAT PER LE MALATTIE INFETTIVE LAZZARO SPALLANZANI IRCCS
Magnetic-Field Measurement Device
InactiveUS20130121879A1Measurement stabilitySimple apparatus configurationComponent separationMaterial analysis by electric/magnetic meansMagnetic markerMeasurement device
The disclosed magnetic immunoassay device, which performs magnetic immunoassays using antigen-antibody reactions, can perform speedy immunoassays without bound / free separation in the test samples. The device is also practical, being capable of stable magnetism measurement without magnetic shielding. The disclosed magnetic immunoassay device is provided with: an excitation coil that uses an AC magnetic field to magnetize a test sample containing a magnetic marker; a magnetism sensor that measures magnetism in the test sample and outputs a magnetism signal; and a displacement sensor for detecting changes in the distance between the test sample and the magnetism sensor. By optimally setting the bandwidth of a lock-in amplifier, which detects changes in the phase of the magnetism signal outputted by the magnetism sensor, and the rotational speed produced by a drive system, which moves the test sample at low speeds, the impact of environment magnetic noise is reduced, and correcting the magnetism signal using distance information obtained from the displacement sensor allows stable magnetism measurement.
Owner:HITACHI LTD
Method and kit for detection of hepatitis a virus neutralizing antibodies
A rapid immunoassay method for the detection of anti-Hepatitis A Virus (HAV) neutralizing antibodies is described herein. This microplate-based enzymatic assay may be applicable in virological diagnostics, in evaluating the immunogenicity of candidate immunogenic compositions, such as HAV vaccines, or in quantifying functional neutralizing antibodies during the course of HAV infection.
Owner:VARIATION BIOTECHNOLOGIES INC
Artificial antigen and specific antibody of veterinary drug penicillin G degradation product benzylpenicilloic thiazole acid
InactiveCN102040661AHigh similarityThe characteristic structure remains intactPeptide preparation methodsDepsipeptidesImmune profilingVeterinary Drugs
An artificial antigen and an antibody of a veterinary drug penicillin G degradation product benzylpenicilloic thiazole acid and preparation method thereof. The invention relates to the preparation of a hapten, an artificial antigen and an antibody of benzylpenicilloic thiazole acid having a structure of (2S, 5R, 6R)-3,3-dimethyl-6-(2-pheylacetamino)-7-oxo-4-thia-1-azabicyclo[3,2,0]heptane-2-formic acid and their application in the establishment of immunoassay. The invention solves the problem that traditional physical and chemical analysis methods are complicated, high in cost and slow in analysis, and provides a simple, quick, sensitive and accurate immunoassay technique. According to the invention, (2S, 5R, 6R)-3,3-dimethyl-6-(2-pheylacetamino)-7-oxo-4-thia-1-azabicyclo[3,2,0]heptane-2-formic acid is adopted as a hapten, and the haptens are linked with KLH and HRP respectively to synthesize artificial antigens and enzyme-labeled antigens. The antibody is prepared by animal immunization, blood drawing, antiserum separation, and purification of artificial antigen. The antibody is stable and has good specificity and sensitivity; the synthetic method is simple; the invention is applicable to the rapid immunoassay of veterinary drug penicillin G degradation product benzylpenicilloic thiazole acid, and has a good application prospect.
Owner:TIANJIN UNIV OF SCI & TECH
Test strip for detecting methylamphetamine and preparation method and application of test strip
InactiveCN108845151AAccurate quantitative contentGuaranteed accuracyBiological testingQuality controlCarrier protein
The invention discloses a test strip for detecting methylamphetamine and a preparation method and an application method of the test strip. The test strip comprises a sample absorption pad, a conjugaterelease pad, a reaction membrane, a water absorption pad and a substrate in sequence, wherein an antibody which is marked by time resolution fluorescent microspheres is sprayed to the conjugate release pad; a detection line of hapten-carrier protein conjugate and a quality control line of a sheep anti-rat secondary antibody are coated on the reaction membrane. By adopting the test strip, the antibody is marked by the time resolution fluorescent microspheres, immunochromatography assay is adopted, and rapid immunoassay of methylamphetamine can be achieved. The invention further provides a method for detecting methylamphetamine in a sample by using the test strip. The test strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate and economic and practical, and rapid detection and on-site detection on large-scale methylamphetamine samples can be achieved.
Owner:郑州左安检测科技有限公司
Reagent strip carrier device used for rapid immunoassay analyzer and use method thereof
The invention discloses a reagent strip carrier device used for a rapid immunoassay analyzer. The reagent strip carrier device is disc-shaped and comprises multiple separable groove-shaped structures used for placing reagent strips. Besides, the invention also discloses a use method for the reagent strip carrier device. The reagent strip carrier device used for the rapid immunoassay analyzer is low in cost and convenient for use, and can flexibly select one or more of (high flux) reagent strips to analyze at one time.
Owner:益思美诠生物科技(上海)有限公司
Rapid immunoassay of anthrax protective antigen in vaccine cultures and bodily fluids by fluorescence polarization
The inventive subject matter relates to a competitive method for estimating the concentration in a sample of a Bacillus anthracis protein or antibody thereof selected from the group consisting of protective antigen (PA), lethal factor (LF) and edema factor (EF). The method may employ the use of Fluorescence Polarization, FLT or FRET. The competitive methods are capable of detecting a target protein within 5 minutes within the range of 0.1 to 10.0 nM. The methods provide for the rapid detection and quantitation of bacteria, bacterial antigen or antibody in culture media or broth of growing cultures of bacteria, including B. anthracis by fluorescent methods.
Owner:THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY
Rapid immunoassay test strip for phytophthora infestans and application thereof
InactiveCN111735954AShort detection timeImprove the efficiency of rapid screeningBiological testingImmunoassaysBiotechnologyCellulose
The invention discloses a rapid immunoassay test strip for phytophthora infestans and application thereof. The test strip comprises a bottom plate, wherein a water absorption pad, a detection pad, a gold label pad and a sample pad are sequentially adhered to one surface of the bottom plate from top to bottom; every two adjacent pads are connected in an overlapped mode at the joint, a nitrocellulose membrane serves as a base pad of the detection pad, a quality control line and a detection line are transversely arranged on the nitrocellulose membrane from top to bottom, the detection line is located below the quality control line, the detection line is coated with an anti-pyricularia grisea polyclonal antibody 0550, and the quality control line is coated with goat anti-mouse IgG. The gold label pad is sprayed with a nanogold labeled anti-pyricularia oryzae monoclonal antibody. The specificity is high, and no cross reaction is caused to pythium aphanidermatum, pear rust water, pear rot, pear anthracnose and the like of Asian pears; the sensitivity of the test strip can reach 1*10<5> cfu / mL, and the test strip can be used for rapid screening of pythium aphanidermatum in fields.
Owner:NANJING AGRICULTURAL UNIVERSITY
Test paper strip for detecting morphine as well as preparation method and application method thereof
InactiveCN108802407AAccurate quantitative contentGuaranteed accuracyBiological testingFluorescenceMicrosphere
The invention discloses a test paper strip for detecting morphine as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, a conjugaterelease pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by time resolved fluorescence microspheres; the reaction membrane is coated with a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the time resolved fluorescence marked antibody and an immunochromatography technique, rapid immunoassay of morphine can be achieved. The invention further provides a method for detecting morphine in a sample by using the test paper strip. The test paper strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of morphine samples can be achieved.
Owner:郑州左安检测科技有限公司
Triple test strip for detecting methamphetamine, morphine and ketamine as well as preparation method and application method of triple test strip
InactiveCN110927395AAccurate quantitative contentGuaranteed accuracyBiological testingAntigenImmune profiling
The invention relates to a triple test strip for detecting methamphetamine, morphine and ketamine as well as a preparation method and an application method of the triple test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody, a morphine antibody and a ketamine antibody marked by the time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine antigen, a detection line T2 of a morphine antigen, a detection line T3 of a ketamine antigen and a quality control line C of a goat anti-mouse secondary antibody or Biotin-BSA. According to the immunochromatographytechnology, the simultaneous and rapid immunoassay of three drugs including methamphetamine, morphine and ketamine is realized. The invention also provides a method for simultaneously detecting the methamphetamine, morphine and ketamine in a sample by using the above test strip. The triple test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.
Owner:郑州左安检测科技有限公司
Electrochemical immunosensor based on DNA functionalized nano composite material
ActiveCN106568828AHigh sensitivityImprove acceleration performanceMaterial electrochemical variablesMesoporous materialDNA hybridisation
The invention discloses an electrochemical immunosensor based on a DNA functionalized nano composite material. The electrochemical immunosensor is formed by the steps: a matrix electrode is loaded with a dendritic metal, then a mesoporous material loaded with an antibody label is added, next a DNA hybrid chain is introduced, and finally, an active substance is added. Preparation comprises the following steps: 1) preparing a matrix material; 2) preparing the mesoporous material loaded with the antibody label; and (3) constructing the electrochemical immunosensor. The invention discloses an application of the electrochemical immunosensor based on the DNA functionalized nano composite material in detection of toxins. Based on a strategy of multiple signal amplification, the electrochemical immunosensor with high sensitivity and low detection limit is constructed. With combination of the high amplification ability of a DNA amplification technology, high sensitivity of electrochemical detection, signal amplification of nano material surface effects and other strategies, the rapid immunoassay is established, and trace toxins are detected.
Owner:SOUTH CHINA AGRI UNIV
A rapid immunoassay for the direct detection of furaltadone metabolite amoz
The invention discloses a fast immunological detection method for directly detecting furaltadone metablolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). The method comprises preparation and application of an AMOZ half antigen, an artificial antigen, an antibody and the like. The AMOZ half antigen has a molecular structure shown as a formula (I) or a formula (II), and the AMOZ artificial antigen has a molecular structure shown as a formula (III) or a formula (IV). An antibody prepared from the antigen can be used for specifically identifying AMOZ. The valence of the antibody is 1:64000, the lowest detection limit is 0.852ng / mL, and the half inhibiting concentration is 15.26ng / mL. The antibody can be directly applied to the detection of AMOZ. By adopting the method, the defect of derivatization of AMOZ in the conventional enzyme linked immunosorbent assay method is overcome; the method is easy, convenient, rapid and low in cost. The antigen and the antibody can be widely applied to residue detection of AMOZ in foods.
Owner:SOUTH CHINA AGRI UNIV
Duplex test strip for detecting morphine and ketamine as well as preparation method and application method of duplex test strip
InactiveCN110927379AAccurate quantitative contentGuaranteed accuracyFluorescence/phosphorescenceImmune profilingMorphine
The invention relates to a duplex test strip for detecting morphine and ketamine as well as a preparation method and an application method of the duplex test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a morphine antibody and a ketamine antibody marked by the time-resolved fluorescentmicrospheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a morphine hapten-carrier protein conjugate, a detection line T2 of a ketamine hapten-carrier protein conjugate and a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of morphine and ketamine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting morphine and ketamine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.
Owner:郑州左安检测科技有限公司
Test strip for detecting 6-monoacetylmorphine as well as preparation method and application method of test strip
InactiveCN110927381AAccurate quantitative contentGuaranteed accuracyFluorescence/phosphorescenceImmune profilingMorphine
The invention discloses a test strip for detecting 6-monoacetylmorphine as well as a preparation method and an application method of the test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, an antibody marked by the time-resolved fluorescent microspheres is sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T of a hapten-carrier protein conjugate and a quality control line C of a goat-anti-mouse secondary antibody. According to the invention, the antibody is labeled by time-resolved fluorescent microspheres, and an immunochromatography technology is adopted to realize the rapid immunoassay of 6-monoacetylmorphine. The invention also provides a method for detecting the 6-monoacetylmorphine in a sample by using the above test strip. The test strip and the method have the advantages of being high in sensitivity and rapid in detection,being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of 6-monoacetylmorphine samples.
Owner:郑州左安检测科技有限公司
Duplex test strip for detecting methamphetamine and ketamine as well as preparation method and application method of duplex test strip
InactiveCN110927380AAccurate quantitative contentGuaranteed accuracyFluorescence/phosphorescenceAntigenImmune profiling
The invention relates to a duplex test strip for detecting methamphetamine and ketamine as well as a preparation method and an application method of the duplex test strip. The test strip sequentiallycomprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody and a ketamine antibody marked by time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine antigen, a detection line T2 of a ketamine antigenand a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of methamphetamine and ketamine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting the methamphetamine and ketamine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.
Owner:郑州左安检测科技有限公司
Test strip for detecting amphetamine and preparation method and application of test strip
InactiveCN108845152AAccurate quantitative contentGuaranteed accuracyBiological testingCarrier proteinHapten
The invention discloses a test strip for detecting amphetamine and a preparation method and application of the test strip. The test strip comprises a sample absorption pad, a conjugate release pad, areaction membrane, a water absorption pad and a substrate in sequence, wherein an antibody which is marked by time resolution fluorescent microspheres is sprayed to the conjugate release pad; a detection line of hapten-carrier protein conjugate and a quality control line of a sheep anti-rat secondary antibody are coated on the reaction membrane. By adopting the test strip, the antibody is marked by the time resolution fluorescent microspheres, immunochromatography assay is adopted, and rapid immunoassay of amphetamine can be achieved. The invention further provides a method for detecting amphetamine in a sample by using the test strip. The test strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate and economic and practical, and rapid detection and on-site detection on large-scale amphetamine samples can be achieved.
Owner:郑州左安检测科技有限公司
Preparation method of nitrocellulose chromatographic membrane for rapid immunoassay, nitrocellulose chromatographic membrane and application thereof
ActiveCN112094424BLess quantityQuick checkBiological material analysisPolymer dissolutionNitrocellulose
The invention provides a method for preparing a nitrocellulose chromatographic membrane for rapid immunoassay, comprising the following steps: (1) preparing a casting solution: dissolving nitrocellulose, cellulose acetate and a hydrophilic polymer in a mixing Solvent, then add surfactant, finally add non-solvent and stir evenly, after defoaming and filtering, cast film solution; (2) scraping film: apply cast film solution on the base material, the wet film thickness of coating is 50-1000μm; (3) Film formation: Evaporate most of the solvent of the wet film after coating in a vacuum chamber, and then undergo a high-temperature non-solvent-induced phase inversion to form a film. The preparation method of the nitrocellulose chromatographic membrane for rapid immunoassay provided by the invention solves the problem in the prior art that the nitrocellulose membrane used for detection is poor in hydrophilicity and uniformity, which affects the detection efficiency.
Owner:浙江美易膜科技有限公司
Human immunodeficiency virus, hepatitis B virus, hepatitis C virus rapid joint detection kit and its preparation and application
ActiveCN105695631BReduced risk of cross-contaminationHigh detection sensitivityMicrobiological testing/measurementMicroorganism based processesFluorescenceImmunodeficiency virus
The invention belongs to the field of biotechnology detection, and particularly relates to a fast joint inspection kit for human immunodeficiency viruses (HIV), hepatitis B viruses (HBV) and hepatitis C viruses (HCV) and preparation and application thereof. The kit comprises an HIV detecting primer and probe, an HBV detecting primer and probe and an HCV detecting primer and probe. A multiplex fluorescent PCR technology is adopted, three kinds of viral nucleic acid of the HIV, the HBV and the HCV are detected in a single PCR reaction tube at the same time, detecting sensitivity is high, good specificity is achieved, the human error rate is low, and time consumed in experiments is short. Fluorescence signals are detected in real time in the amplified reaction process, the whole process is conducted in a sealed mode, the risk of cross infection among samples is reduced, and the kit is suitable for being applied to large-scale blood screening and clinical examinations.
Owner:INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU +1
Micro-fluidic chip for micro-magnetic field control and manufacturing method thereof
InactiveCN101879467BEasy to prepareEasy to operateLaboratory glasswaresChemical/physical/physico-chemical processesMagnetic field gradientMagnetic bead
The invention discloses a micro-fluidic device for micro-magnetic field control and a manufacturing method thereof. The device consists of a micro-fluidic fluid layer chip, a substrate provided with soft magnetic material micro-patterns, and a magnetic source body. The substrate provided with the soft magnetic material micro-patterns is a transparent conducting substrate, wherein the micro-patterns are arranged and designed corresponding to needed magnetic beads, and a layer of a polymeric material is covered on the micro-patterns to realize a connection in a permanent irreversible bonding mode with the micro-fluidic fluid layer chip. Under the action of an external magnetic field, nickel micro-patterns are magnetized and generate a strong local magnetic field gradient to realize the magnetic field control in a micron range. The device can capture magnetic substances at a high flow speed, and can release the magnetic substances at a low flow speed after the magnetic source body is removed. The device can be used in the fields of organism-targeted material detection, gene analysis, quick immunoassay, cell screening and the like.
Owner:WUHAN UNIV
Duplex test strip for detecting methamphetamine and morphine as well as preparation method and application method of duplex test strip
InactiveCN110927396AAccurate quantitative contentGuaranteed accuracyBiological testingImmune profilingMorphine
The invention relates to a duplex test strip for detecting methamphetamine and morphine as well as a preparation method and an application method of the duplex test strip. The test strip sequentiallycomprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody and a morphine antibody which are marked by the time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine hapten-carrier protein conjugate, adetection line T2 of a morphine hapten-carrier protein conjugate and a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of methamphetamine and morphine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting the methamphetamine and morphine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being highin sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.
Owner:郑州左安检测科技有限公司
A novel stool collection and rapid immunoassay device and its operating method
Owner:ZHUHAI ENCODE MEDICAL ENG
An electrochemical immunosensor based on DNA-functionalized nanocomposites
ActiveCN106568828BHigh sensitivityImprove acceleration performanceMaterial electrochemical variablesImmune profilingEngineering
The invention discloses an electrochemical immunosensor based on a DNA functionalized nano composite material. The electrochemical immunosensor is formed by the steps: a matrix electrode is loaded with a dendritic metal, then a mesoporous material loaded with an antibody label is added, next a DNA hybrid chain is introduced, and finally, an active substance is added. Preparation comprises the following steps: 1) preparing a matrix material; 2) preparing the mesoporous material loaded with the antibody label; and (3) constructing the electrochemical immunosensor. The invention discloses an application of the electrochemical immunosensor based on the DNA functionalized nano composite material in detection of toxins. Based on a strategy of multiple signal amplification, the electrochemical immunosensor with high sensitivity and low detection limit is constructed. With combination of the high amplification ability of a DNA amplification technology, high sensitivity of electrochemical detection, signal amplification of nano material surface effects and other strategies, the rapid immunoassay is established, and trace toxins are detected.
Owner:SOUTH CHINA AGRI UNIV
An FITC test paper strip for detecting benzodiazepine, a preparing method thereof and an application method of the test paper strip
InactiveCN108918896AAccurate quantitative contentGuaranteed accuracyBiological testingFluorescence/phosphorescenceBenzodiazepineQuality control
An FITC test paper strip for detecting benzodiazepine, a preparing method thereof and an application method of the test paper strip are disclosed. The test paper strip includes a sample absorption pad, a conjugate releasing pad, a reaction film, a water absorbing pad and a bottom plate in order. The conjugate releasing pad is sprayed with an FITC labeled antibody. The reaction film is coated witha hapten-carrier protein conjugate detection line and a goat anti-mouse secondary antibody quality control line. The FITC labeled antibody is utilized to achieve benzodiazepine rapid immunoassay through immunochromatography. The invention also provides the application method for detecting benzodiazepine in a sample by utilizing the test paper strip. The test paper strip has advantages of high sensitivity, capability of accurate quantification, rapid detection, convenient operation, and capability of being practical and economical, and can achieve rapid detection and on-site detection for large-batch benzodiazepine samples.
Owner:郑州左安检测科技有限公司
Rapid immunoassay test strip for bacterial fruit blotch II type strains of melons and application thereof
InactiveCN111735953AShort detection timeImprove the efficiency of rapid screeningBiological testingImmunoassaysBiotechnologyCellulose
The invention discloses a rapid immunoassay test strip for bacterial fruit blotch II type strains of melons and application thereof. The test strip comprises a bottom plate, wherein a water absorptionpad, a detection pad, a gold label pad and a sample pad are sequentially adhered to one surface of the bottom plate from top to bottom; every two adjacent pads are connected in an overlapped mode atthe joint, a nitrocellulose membrane serves as a base pad of the detection pad, a quality control line and a detection line are transversely arranged on the nitrocellulose membrane from top to bottom,the detection line is located below the quality control line, the detection line is coated with an anti-cucurbit bacterial fruit blotch germ polyclonal antibody 1152, and the quality control line iscoated with goat anti-mouse IgG. The gold label pad is sprayed with a nanogold-labeled monoclonal antibody for resisting bacterial fruit blotch II type strains of melons. The test strip is high in specificity, capable of distinguishing bacterial fruit blotch type I strains and bacterial fruit blotch type II strains of melons, free of cross reaction with bacterial angular leaf blotch and the like,capable of achieving the sensitivity of 1*10<5> cfu / mL and capable of being used for rapid screening of the bacterial fruit blotch type II strains of the melons in the field.
Owner:NANJING AGRICULTURAL UNIVERSITY
Preparation of visible colored insoluble carrier particles labeled with a fluorescent dye and an immunoassay method using the same
InactiveUS20190361029A1Improve accuracyBiological testingFluorescence/phosphorescenceVisual observationFluorochrome Dye
The object of the present invention is to provide insoluble carrier particles used for high-sensitivity rapid immunoassay, which allow visual observation and high-sensitivity apparatus measurement.The insoluble carrier particles are visible colored insoluble carrier particles labeled with a fluorescent dye, which are used for immunoassay, wherein absorption of fluorescence by the visible colored insoluble carrier particles is low within a wavelength range of fluorescence emitted by the fluorescent dye.
Owner:DENKA CO LTD
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com