10 results about "Rapid immunoassay" patented technology

The invention discloses a preparation method and application of a colloidal gold immunochromatography test strip for detecting IDH1 mutant protein, and belongs to the field of rapid immunodetection. The colloidal gold chromatography test strip comprises a bottom plate, a sample pad, a nitrocellulose membrane, a water absorption pad and colloidal gold, the nitrocellulose membrane is sequentially provided with a detection line T and a quality control line C in the flowing direction of a sample, the detection line T is coated with IDH1 mutant protein, and the quality control line C is coated with a second antibody corresponding to a monoclonal antibody of the IDH1 mutant protein. Clinical tumor tissue samples collected in real time in glioma resection surgery are treated, an object to be detected and an immune gold label are mixed, incubated and dropped in a test strip sample adding hole, and the object to be detected runs along the test strip under the driving of a buffer solution and generates visual color change. The test strip has the advantages of being rapid in reaction, easy and convenient to operate, convenient to carry, high in specificity and the like, makes up the blank of an IDH1 mutant protein concentration quantitative method, and has good clinical application value.

The invention discloses a multifunctional glass microbubble, a high-flux rapid immunoassay system and application thereof, and the multifunctional glass microbubble is prepared by the following steps: S1, adding a bovine serum albumin solution into hollow glass microbubbles to obtain a bovine serum albumin coating, centrifuging, removing a lower-layer solution, cleaning and drying to obtain a hollow glass microbubble solution; the antifouling hollow glass microbubbles are obtained; s2, mixing the antifouling hollow glass microbubbles with tetrazine-active ester, centrifuging, removing a lower solution, washing and drying to obtain the antifouling hollow glass microbubbles with the biological orthogonal function. The anti-fouling hollow glass microbubble has the beneficial effects that the anti-fouling hollow glass microbubble with a biological orthogonal function, the self-suspension performance, the anti-fouling function and the bovine serum albumin rich in tyrosine residues not only can realize high-flux array detection in a 96-well plate, but also can realize the effects of anti-fouling and signal amplification, and can be applied to the field of biosensor detection. Therefore, the defects of low flux, low sensitivity, long detection time and the like of a traditional detection method are overcome.

This invention provides a specific detection method for *Daphnia magna* in nearshore marine aquatic ecosystem assessment. Based on obtaining the species-specific antigenic protein and key immunogenic peptides of *Daphnia magna*, a highly specific monoclonal antibody was successfully prepared and purified, and a colloidal gold side-flow chromatography rapid detection test strip was prepared using this antibody. The amino acid sequence of the antigenic protein is SEQ ID NO:1 or SEQ ID NO:2. This invention, by screening for the *Daphnia magna* species-specific antigen and its corresponding monoclonal antibody, combined with paired antibodies prepared from the full-length protein, and using them to develop a colloidal gold rapid immunoassay product, achieves for the first time high-throughput, rapid on-site screening of this species, significantly improving the efficiency and accuracy of marine ecological monitoring, ship ballast organism inspection, and aquaculture environmental assessment.

This invention discloses a method and system for detecting the strongest Rayleigh acoustic current in immobilized microdroplets. Specifically, it involves: calculating the acoustic current suppression coefficient through experimental quantitative testing to establish a quantitative mapping relationship between acoustic current intensity and droplet contact angle; utilizing an electrowetting structure composed of interdigitated electrodes, a dielectric layer, and a hydrophobic layer to actively regulate the droplet contact angle through an applied voltage; and dynamically adjusting the applied voltage in real time by monitoring the droplet vibration amplitude, particle velocity, and contact angle through a collaborative control program to maintain the acoustic current intensity at its strongest. This invention also provides a detection system for implementing the above method, including an indium tin oxide transparent interdigitated electrode substrate, a lead zirconate titanate piezoelectric transducer, a laser Doppler vibrometer, a microscope, and a collaborative control module. This invention can maximize and dynamically maintain the Rayleigh acoustic current intensity in immobilized microdroplets, significantly accelerating the solid-liquid interface mass transfer process, and is applicable to biochemical analysis fields such as rapid immunoassay and biological particle mixing.

The utility model discloses a rapid immunodetection device, and aims to solve the technical problem that in the prior art, a test box or test paper for sampling is inconvenient to be placed at a proper temperature, so that the waiting time of the test box is prolonged. The device comprises a bottom plate, the top surface of the bottom plate is fixedly connected with a supporting plate, the supporting plate is arranged in a U shape, the inner side of the supporting plate is rotatably connected with a round shell, the side wall of the round shell is fixedly connected with a plurality of placing boxes, the right side wall of each placing box is provided with an opening, and the side wall of the side, away from the center of the round shell, of each placing box is provided with a through groove; a heating pipe is installed in an inner cavity of the round shell, and a shielding assembly is arranged on the side wall of the round shell and located on the inner side of the containing box. A metal plate is fixedly connected to the side wall of one side of the supporting plate, and a plurality of magnetic blocks are attracted to the side wall of the metal plate. The surface of the round shell is in a proper temperature interval by utilizing the heating pipe, so that the change time of the test box is shortened, and the immunodetection rate is improved.

The invention discloses a cefalonium hapten, an antigen, an antibody as well as a preparation method and application thereof, and relates to the cefalonium hapten, the antigen, the antibody as well as the preparation method thereof and the application in detecting cefalonium residues. The cefalonium hapten and the artificial antigen as shown in the formula (I) are prepared by using a total synthesis method, and a specific antibody for detecting cefalonium is further prepared. The cross reaction rate of the antibody prepared from the hapten / antigen to common structural analogues is lower than 0.01%, the accuracy is high, the interference of other structural analogue drugs can be effectively eliminated, and a basic raw material is provided for subsequent further development of an immune rapid detection technology and a product aiming at cefalonium. # imgabs0 # is represented by formula (I).

A time-resolved fluorescence microsphere immunochromatographic test strip for detecting cannabidiol, its preparation method and application. The test strip includes a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate. The conjugate release pad is sprayed with a monoclonal antibody against cannabidiol labeled with time-resolved fluorescence microspheres. The reaction membrane is fixed with a test line and a quality control line. The test line is coated with a cannabidiol hapten-carrier protein conjugate, and the quality control line is coated with goat anti-mouse secondary antibody. The monoclonal antibody against cannabidiol is obtained by using the cannabidiol-hapten carrier protein conjugate as an immunogen. The cannabidiol hapten retains the characteristic structure of cannabidiol to the greatest extent. The present invention uses time-resolved fluorescence microspheres to label antibodies and adopts immunochromatographic technology to realize rapid immunoassay of cannabidiol in samples, with high sensitivity, accurate quantification, rapid detection and convenient operation, and can realize rapid detection and on-site detection of a large number of samples.

The invention discloses a time-resolved fluorescent quantitative test strip for detecting alternaria alternata in tobacco leaves as well as a preparation method and application of the test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction film, a water absorption pad and a bottom plate, the kit is characterized in that a detection antibody marked by time-resolved fluorescent microspheres is sprayed on the conjugate release pad; the reaction film is coated with a detection line for capturing an antibody and a quality control line for a goat-anti-mouse second antibody; according to the invention, a detection antibody is marked by time-resolved fluorescent microspheres, and rapid immunoassay of alternaria alternata in tobacco leaves is realized by adopting an immunochromatography technology. The invention also provides a method for detecting the content of alternaria alternata in tobacco leaves by using the test strip. The method has the advantages of high sensitivity, accurate quantification, rapid detection, convenient operation, economy and practicability, and can realize rapid detection and on-site detection of a large batch of tobacco leaf samples.

This invention discloses a detection system and method for NbY24 conjugated with carbon quantum dots, belonging to the field of immunoassay technology. The detection system for NbY24 conjugated with carbon quantum dots provided by this invention includes the following components: a CQDs-NbY24 solution prepared by bioconjugating NbY24 with carbon quantum dots; and a standard curve established with the fluorescence quenching efficiency calculated from the fluorescence intensity of the CQDs-NbY24 solution and the fluorescence intensity of the CQDs-NbY24 solution mixed with a gradient concentration of AFM1 solution as the ordinate and the AFM1 solution concentration as the abscissa. This invention employs a fluorescence signal amplification strategy, conjugating NbY24 with CQDs, to establish a highly sensitive, selective, simple, and rapid immunoassay method based on FIA for the detection of AFM1 in dairy products, providing a reference for future monitoring of AFM1 contaminants.

The invention provides a homogeneous rapid immunoassay method for circulating tumor cells by using a metal ion mediated self-assembly functional nucleic acid nano-machine, relates to the technical field of biomedical diagnosis, and is used for establishing a simple, rapid, sensitive and high-sensitivity immune sensor system from the aspects of construction of a hypersensitive homogeneous immunosensing system, application of the metal ion functional nucleic acid nano-machine and the like. On the basis of a circulating tumor cell analysis strategy of various surface markers, expensive instruments or reagents are not needed, all materials are prepared in advance, the detection time is within 1 hour, multi-target simultaneous high-sensitivity detection under the room temperature condition is realized, and compared with a clinical imaging and pathology'gold standard 'detection result, the consistency reaches 90% or above. According to the invention, a medical diagnosis system can be enriched, and technical support is provided for rapid, non-invasive and accurate tumor diagnosis.