2 results about "Rapid immunoassay" patented technology

This invention provides a specific detection method for *Daphnia magna* in nearshore marine aquatic ecosystem assessment. Based on obtaining the species-specific antigenic protein and key immunogenic peptides of *Daphnia magna*, a highly specific monoclonal antibody was successfully prepared and purified, and a colloidal gold side-flow chromatography rapid detection test strip was prepared using this antibody. The amino acid sequence of the antigenic protein is SEQ ID NO:1 or SEQ ID NO:2. This invention, by screening for the *Daphnia magna* species-specific antigen and its corresponding monoclonal antibody, combined with paired antibodies prepared from the full-length protein, and using them to develop a colloidal gold rapid immunoassay product, achieves for the first time high-throughput, rapid on-site screening of this species, significantly improving the efficiency and accuracy of marine ecological monitoring, ship ballast organism inspection, and aquaculture environmental assessment.

This invention discloses a method and system for detecting the strongest Rayleigh acoustic current in immobilized microdroplets. Specifically, it involves: calculating the acoustic current suppression coefficient through experimental quantitative testing to establish a quantitative mapping relationship between acoustic current intensity and droplet contact angle; utilizing an electrowetting structure composed of interdigitated electrodes, a dielectric layer, and a hydrophobic layer to actively regulate the droplet contact angle through an applied voltage; and dynamically adjusting the applied voltage in real time by monitoring the droplet vibration amplitude, particle velocity, and contact angle through a collaborative control program to maintain the acoustic current intensity at its strongest. This invention also provides a detection system for implementing the above method, including an indium tin oxide transparent interdigitated electrode substrate, a lead zirconate titanate piezoelectric transducer, a laser Doppler vibrometer, a microscope, and a collaborative control module. This invention can maximize and dynamically maintain the Rayleigh acoustic current intensity in immobilized microdroplets, significantly accelerating the solid-liquid interface mass transfer process, and is applicable to biochemical analysis fields such as rapid immunoassay and biological particle mixing.