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This invention discloses an anti-inflammatory peptide derived from pearl oysters and its applications, relating to the field of bioactive peptide technology. The amino acid sequence of this anti-inflammatory peptide is TAMY. The anti-inflammatory peptide derived from pearl oysters provided by this invention can regulate the release of cellular inflammatory mediators, reduce the levels of NO and pro-inflammatory cytokines (such as TNF-α, IL-6, and IL-1β), and simultaneously promote the secretion of anti-inflammatory cytokines (such as IL-10), exhibiting excellent anti-inflammatory activity and suitable for use in functional foods, cosmetics, or pharmaceutical ingredients.