Injectable self-sealing gel applicable to drug loading and releasing and preparation method and application thereof

An injection-type, self-healing technology, applied in pharmaceutical formulation, drug delivery, medical science, etc., can solve problems such as weak mechanical strength, inability to accurately control drug release, difficulty in taking into account biocompatibility and biodegradability, etc.

Active Publication Date: 2017-08-04
SHENZHEN HUA NOVA BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these self-healing materials are prone to microscopic phase separation due to the widespread use of intermolecular interactions between polymer chains, including hydrogen bonds, coordination of metal ion ligands, electrostatic interactions, or hydrophobic interactions, and such water Gel polymer network can not realize the controllable loading and release of drug molecules
At the same time, traditional self-healing polymer hydrogel materials have many insurmountable bottlenecks, including weak mechanical strength, inability to accurately control drug release, and difficulty in balancing biocompatibility and biodegradability
These have limited its application in the field of biomedicine, especially as implantable biomaterials

Method used

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  • Injectable self-sealing gel applicable to drug loading and releasing and preparation method and application thereof
  • Injectable self-sealing gel applicable to drug loading and releasing and preparation method and application thereof
  • Injectable self-sealing gel applicable to drug loading and releasing and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Using type A gelatin as raw material, heat it in deionized water at 40°C to dissolve it, prepare a gelatin aqueous solution with a concentration of 5w / v%, adjust the pH value of the gelatin aqueous solution to 3, and then add acetone 3 times the volume of the solution to the solution to generate The dispersion of gelatin microgel particles; adding different amounts of 25wt% glutaraldehyde aqueous solution to the dispersion respectively to crosslink the gelatin microgel particles, the amount of glutaraldehyde added is respectively 25wt% glutaraldehyde per gram of gelatin Aldehyde 66μL, 132μL, 264μL, 538μL, cross-linking reaction time 12hr, then add glycine to neutralize the unreacted aldehyde group, centrifuge and wash to obtain the dispersion liquid of type A gelatin microgel particles.

[0048] The particle size analysis of type A gelatin particles prepared with different crosslinking agent dosages was carried out using a laser particle size analyzer, and the results ar...

Embodiment 2

[0054]Use type A gelatin and type B gelatin as raw materials respectively, dissolve them in deionized water and heat at 40°C, and prepare an aqueous solution of type A gelatin with a concentration of 5w / v% and type B gelatin with a concentration of 5w / v% aqueous solution, adjust the pH value of the two gelatin aqueous solutions to be 3, and then add 3 times the volume of acetone to the solution to generate type A gelatin and type B gelatin microgel particle suspensions; add 25wt% pentadiene to the suspension respectively Aldehyde aqueous solution, to cross-link the gelatin microgel particles, the amount of glutaraldehyde added is 66 μL of 25wt% glutaraldehyde per g gelatin, the cross-linking reaction time is 12hr, then add glycine to neutralize the unreacted aldehyde groups, centrifuge and wash separately A type A gelatin particle dispersion and a type B gelatin particle dispersion were obtained. The particle size and zeta potential data of the prepared microgel measured by la...

Embodiment 3

[0067] Use type A gelatin as the raw material, dissolve it under heating at 40°C, prepare a type A gelatin aqueous solution with a concentration of 5w / v%, adjust the pH value to 11, and then add 3.5 times the volume of ethanol to the solution to generate type A gelatin Microgel particle suspension; add 25wt% glutaraldehyde aqueous solution to the suspension to cross-link the gelatin microgel particles. The amount of glutaraldehyde added is 66 μL of 25wt% glutaraldehyde per gram of gelatin. After 12 hours, type A gelatin microgel particles were obtained, and the particle size and surface zeta point parameters are shown in Table 5.

[0068] Prepare alginate microgel particles by emulsion method, the specific preparation method is as follows: add 1wt% sodium alginate aqueous solution into calcium chloride aqueous solution and continue high-speed stirring (stirring speed>5000rpm), that is, calcium alginate particles, particles The size and surface zeta point parameters are shown i...

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Abstract

The invention discloses injectable self-sealing gel applicable to drug loading and releasing and a preparation method and application thereof. Macromolecular microgel particles with surface charge are used herein, and colloidal gel is obtained by self-assembly through static mutual action of the particles. Since static actions in the microgel are physically crosslinked and are reversible; the colloidal gel of the invention has good injectability and self-healing property, no chemical crosslinking reaction needs to be introduced during injection and curing, the gel is different from chemical macromolecules and is free of small-molecular crosslinking agents, and the gel is good in biocompatibility, degradable and absorbable, free of toxic and side effects and good in safety and is possibly applicable widely to the field of biomedicine.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to an injectable self-healing gel biomaterial, which can be used for drug loading and release, and its preparation method and application. Specifically, the present invention relates to a biocompatible, degradable colloid gel material based on gelatin particles, which can be directly applied to people and other mammals caused by surgical operations (including minimally invasive operations) and trauma. Repair filling and regenerative reconstruction of tissue and organ wounds or defects. Background technique [0002] Self-healing materials are a new type of functional materials, which can automatically repair and heal after being damaged by external forces, and can completely or partially restore the structure and mechanical strength before the damage, and have become a very important class of new, intelligent, engineering materials. Material. Among them, hydrogel materia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/22A61L27/50A61L27/52A61L27/54A61L27/58B33Y70/00B33Y80/00
CPCA61L27/18A61L27/20A61L27/222A61L27/50A61L27/52A61L27/54A61L27/58A61L2300/414A61L2300/604A61L2400/06B33Y70/00B33Y80/00C08L5/04C08L71/02
Inventor 王华楠
Owner SHENZHEN HUA NOVA BIOTECH LTD
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