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Preparation method of multi-growth-factor slow release coating of titanium and titanium alloy surface

A technology of growth factors and titanium alloys, which is applied in the fields of surface coating liquid devices, coatings, medical science, etc., can solve the problems of unstable coating structure, slow release of growth factors, etc., and achieve convenient slow release time and Sequential, high immobilization, high activity effect

Inactive Publication Date: 2015-02-11
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it can maintain the activity of growth factors to the greatest extent and control the release sequence of different growth factors; however, the amount of growth factors that can be loaded in polymer materials is relatively small, and the interpenetration of polymer materials between layers makes the self-assembled coating The layer structure is unstable, and it is difficult to stably regulate the sustained release of various growth factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of a multi-growth factor sustained-release coating on a titanium surface, the specific steps of which are as follows:

[0029] A. Loading of growth factors:

[0030] A1, the concentration of configuration chitosan (polycation) and dopamine is the mixed solution of 1mg / mL;

[0031] A2, four kinds of growth factors of BMP-2, VEGF, FGF and TGF-β are dissolved in the heparin (polyanion) solution that concentration is 1mg / mL respectively, obtain the growth factor containing BMP-2, VEGF, VEGF and TGF-β respectively Factor four groups of heparin solution, the concentration of growth factor in each group of heparin solution is 5μg / mL;

[0032] A3, the four groups of polyanion solutions of A2 step are mixed with the mixed solutions of A1 step in a volume ratio of 4:1, and stirred, centrifuged, and freeze-dried to prepare four groups of negatively charged nanoparticles; four groups of negatively charged nanoparticles The charged nanoparticles were loaded wi...

Embodiment 2

[0040] A method for preparing a multi-growth factor sustained-release coating on the surface of a titanium alloy, the specific steps of which are as follows:

[0041] A. Loading of growth factors:

[0042] A1, the concentration of configuration polylysine (polycation) and dopamine is the mixed solution of 2mg / mL;

[0043]A2, two kinds of growth factors of BMP-2 and TGF-β are dissolved in the sodium alginate (polyanion) solution that concentration is 2mg / mL respectively, obtain two groups of alginic acid containing BMP-2 and TGF-β growth factor respectively Sodium solution, the concentration of growth factors in each group of sodium alginate solution is 10 μg / mL;

[0044] A3, the two groups of sodium alginate (polyanion) solutions in step A2 are mixed with the mixed solution in step A1 at a volume ratio of 5:1, and stirred, centrifuged, and freeze-dried to prepare BMP-2 loaded with growth factors respectively. and TGF-β two groups of negatively charged nanoparticles;

[0045...

Embodiment 3

[0052] A preparation method of a multi-growth factor sustained-release coating on a titanium surface, the specific steps of which are as follows:

[0053] A. Loading of growth factors:

[0054] A1, the concentration of configuration chitosan (polycation) and dopamine is the mixed solution of 0.5mg / mL;

[0055] A2, two kinds of growth factors of BMP-2 and FGF are dissolved in the hyaluronic acid solution that concentration is 0.5mg / mL respectively, obtain two groups of hyaluronic acid solutions containing BMP-2 and FGF growth factor respectively, each group of hyaluronic acid The concentration of growth factors in the acid (polyanion) solution is 0.5 μg / mL;

[0056] A3. Mix the two groups of hyaluronic acid solutions in step A2 with the mixed solutions in step A1 at a volume ratio of 3:1, and stir, centrifuge, and freeze-dry to prepare two groups of negatively charged nanoparticles, one of which is The nanoparticles were loaded with the growth factor BMP-2, and another set wa...

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Abstract

The invention discloses a preparation method of a multi-growth-factor slow release coating of a titanium and titanium alloy surface. According to the method, a plurality of layers of coatings containing nanoparticles of growth factors are prepared on the titanium surface by taking the nanoparticles as a growth factor carrier and charged high molecular thin films as a medium. The nanoparticles included in the coating are prepared by mixing polycation solution containing dopamine of certain concentration with polyanion solution containing the growth factors. The nanoparticles have good dispersibility, can protect the activity of the growth factors and are easy to fix on the surface of a material. By controlling the electric property of the nanoparticles and the high molecular thin films, nanoparticles carrying with different factors are embedded between the high molecular thin films. By adjusting the loading quantity and the loading sequence of different nanoparticles and the thickness of the coatings, effective control over release speed, release quantity and release sequence of the various growth factors can be realized.

Description

technical field [0001] The invention relates to a method for preparing slow-release coatings of various growth factors on the surface of medical titanium and titanium alloys. Background technique [0002] Titanium and titanium alloys have been widely used in orthopedics and dentistry due to their good biocompatibility and excellent mechanical properties. However, titanium and titanium alloys are biologically inert materials. After being implanted into the human body, the regeneration of new bone on the surface is slow, and the bonding between the new bone and the titanium surface is weak. In order to improve the surface activity and osseointegration ability of titanium implants, it is often necessary to functionalize the titanium surface. Commonly used methods to improve biological activity on titanium surfaces include alkali heat treatment, anodic oxidation, hydroxyapatite coating, and loading of biomolecules and specific growth factors that promote cell adhesion and growt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B05D7/14B05D7/24A61L27/40A61L27/34A61L27/54
Inventor 鲁雄王振铭翁杰冯波屈树新汪建新
Owner SOUTHWEST JIAOTONG UNIV
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