Sticking agent for making bacterial scanning electron microscope observation slice and preparation method thereof

Abstract

A sticking agent for making a bacterial scanning electron microscope observation slice and a preparation method thereof are disclosed; every 100 ml of the sticking agent comprises 3-5mL of egg white, 5-7mL of glutinous rice powder emulsion, and balance of distilled water; the preparation method is as follows: respectively taking 3-5mL of the egg white and 5-7mL of the glutinous rice powder emulsion to put into a beaker, using sterile water to fix the volume to 100mL, evenly mixing, using a disposable needle filter with the specification of 0.22 mu m for filtering to obtain filtrate as the sticking agent; a method for making the scanning electron microscope bacterial ultramicroscopic morphology observation slice from the sticking agent comprises the steps of collection of bacteria, fixation, sticking, dehydration, replacement, critical point drying, sticking on a sample platform and ion sputtering; the effective sticking agent aiming at bacterial scanning electron microscope observation experiments and the preparation method thereof and a slice preparation method are provided, and the effective sticking agent solves slice shedding and mixing phenomena in the bacterial scanning electron microscope observation experiments.

Description

technical field [0001] The invention relates to a tablet adhesive, in particular to a tablet adhesive for making bacterial scanning electron microscope observation sheets and a preparation method thereof. Background technique [0002] Microorganisms cannot be directly observed by the naked eye because of their tiny size, so microscopic observation of bacteria is the most basic skill in microbial research. Electron microscope) to understand the ultra-microscopic morphological characteristics of the bacteria. Bacteria are the most typical type of organisms in microorganisms. In traditional bacterial scanning electron microscope observation experiments, there have been no reports of using adhesive tablets. The classic method is to use the natural adhesion of bacteria to glass slides to complete the experiment. However, this treatment method has a high rate of detachment of the bacteria, especially in the critical drying of carbon dioxide, because the repeated impact of carbon ...

AI Technical Summary

Problems solved by technology

[0003] The traditional bacterial scanning electron microscope observation and production technology can generally achieve the experimental purpose, but the existing problems are also obvious. These shortcomings are concentrated in: (1) The adhesion of bacteria on the slide depends only on the natural force of the bacterial cell wall on the slide. Adhesion, the obvious peeling phenomenon occurs in the dehydration, replacement and carbon dioxide drying process of film production; (2) When the film peeling phenomenon occurs in the carbon dioxide drying process, it is easy to form a mixture of different samples

Although only drying one sample at a time can avoid the occurrence of confounding, this greatly increases the time and cost of the experiment, which is not conducive to the development of multiple sample experiments

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