36 results about "Growth factor activity" patented technology

Modulation of the glucocorticoid inducible kinases to restore Connective tissue growth factor activity. Also disclosed are methods and compounds useful for the detection and treatment of fibroproliferative disorders.

A canine hepatocyte growth factor gene and a 15 base pairs-deleted gene thereof encode a protein having an amino acid sequence shown in SEQ ID NO: 2 or 4 and a protein having an amino acid sequence comprising a deletion, substitution or addition of one or several amino acids and having a canine hepatocyte growth factor activity. The canine hepatocyte growth factor and the 5 amino acids-deleted canine hepatocyte growth factor are useful for the treatment of liver diseases, kidney diseases, lung diseases, digestive diseases, cardiocirculatory diseases or cranial nerve diseases.

The present invention relates to a growth factor-mimicking peptide having an activity of the growth factor, and a composition and a method for improving skin conditions or for treating a wound using the same. The growth factor-mimicking peptides of this invention have identical functions or actions to natural-occurring human growth factor, and much better stability and skin penetration potency than natural-occurring growth factor. In these connections, the composition comprising the peptides of this invention can exhibit excellent efficacies on the treatment, prevention and improvement of diseases or conditions demanding growth factor activities. In addition, excellent activity and stability of the peptides of this invention can be advantageously applied to pharmaceutical compositions, quasi-drugs and cosmetics.

The invention discloses a growth factor-loaded micro-nano composite membrane. The micro-nano composite membrane is composed of a growth factor-loaded egg shell membrane, a protein membrane, a hydrophilic micro-nano fiber layer and a hydrophobic micro-nano fiber layer; the micro-nano composite membrane takes the egg shell membrane as a direct carrier of a growth factor, and meanwhile protein servesas a coating layer to be deposited on the surface of the growth factor-loaded egg shell membrane to form the protein membrane through salting out, and then the hydrophilic micro-nano fiber membrane and the hydrophobic micro-nano fiber membrane wrap the surface of the protein membrane in sequence through an electrostatic spinning technology; the protein membrane is obtained through salting-out sedimentation by adopting poultry egg white. The micro-nano composite membrane is reasonable in structural design, so that the loaded growth factor has the slow-release property, and the materials forming the membrane layers have the effect of keeping the growth factor activity.

A number of peptides of from 28 to 44 amino acids, including therein a sequence of L-K-E-K-K (SEQ ID NO: 1) have a physiological activity that can be wound healing activity, immunostimulant activity, or growth factor activity. The peptides can be linear or circular, and if linear, the amino terminus can be optionally acetylated. The invention also includes nucleic acids encoding these peptides, vectors incorporating these nucleic acid sequences, and host cells transfected with these vectors, as well as methods for producing these peptides by culturing these host cells and purifying these peptides. The invention further includes pharmaceutical compositions and methods for using these peptides.

The invention discloses a preparation method of a multi-growth-factor slow release coating of a titanium and titanium alloy surface. According to the method, a plurality of layers of coatings containing nanoparticles of growth factors are prepared on the titanium surface by taking the nanoparticles as a growth factor carrier and charged high molecular thin films as a medium. The nanoparticles included in the coating are prepared by mixing polycation solution containing dopamine of certain concentration with polyanion solution containing the growth factors. The nanoparticles have good dispersibility, can protect the activity of the growth factors and are easy to fix on the surface of a material. By controlling the electric property of the nanoparticles and the high molecular thin films, nanoparticles carrying with different factors are embedded between the high molecular thin films. By adjusting the loading quantity and the loading sequence of different nanoparticles and the thickness of the coatings, effective control over release speed, release quantity and release sequence of the various growth factors can be realized.

The invention relates to a collagen-based bilayer membrane material for directional release of growth factors and a manufacturing method of the collagen-based bilayer membrane material. The collagen-based bilayer membrane material is prepared from collagen, the growth factors, fibrinogen and thrombin; a dense layer collagen membrane and a loose layer collagen membrane are prepared, and the dense layer collagen membrane and the loose layer collagen membrane are bonded by virtue of fibrous protein, so that a bilayer membrane structure is constituted. The growth factors can be loaded on the surface of the membrane by a dosage of 1-10000ng of the growth factors per square centimeter; preferably, the growth factors are loaded on the bilayer membrane by a dosage of 30ng per square centimeter; and preferably, average thickness of the bilayer membrane is 1-5mm. The material has a bidirectional controlled-release effect on the loaded growth factors, so that directional release of the growth factors is achieved and the material can act on target tissues; and the material is applicable to restorative treatment of diseased and defective tissues. The growth factors, which are loaded on the bilayer collagen membrane prepared by the invention and have a targeted release effect, can be released to the target tissues in a directional mode and activity of the growth factors can be kept; and the bilayer membrane material can be used for guiding and inducing regeneration and repair of tissues.

This invention relates to a kind of protein that possesses erythropoietin activity and its preparation. This protein is derivatized through its amino acid sequence is substituted by one or more amino acid residue, absence or addition ,in the condition that remaining tertiary structure of pleckstrin homology structural domain to be invariant, it possesses erythropoietin activity. Technology project of this invention is that function of haemopoietin EPO is grafted on backbone of pleckstrin homology structural domain by using protein functional grafting method, progress mutant design, expression and depuration, and determine the binding capability of mutant and EPO acceptor and EPO activity by experiment. This invented protein can be used as EPO succedaneum, used for curing anaemia, chronic renal failure(CRF),HIV infected / ZDU curing patient, arthritis deformans, carcino-anaemia and any other symptoms that can be cured by using EPO.

The invention discloses a growth factor-loaded collagen group composite material and a preparation method thereof. The growth factor-loaded collagen group composite material is prepared from collagen, a growth factor, chitosan and sodium heparin, a dense layer collagen film and a loose layer collagen film form a double-layer structure, and a growth factor-loaded chitosan-heparin nano particle is compounded between the double layers. The dosage of the growth factor is 50-10,000ng / cm<2>; and the average thickness of the double-layer film is 1-4mm. The preparation method comprises the steps of preparing the dense collagen film and the loose collagen film, preparing the growth factor-loaded nano particle, compounding the collagen films and the nano particle, and the like. The collagen group composite material prepared by the invention has orient difference control slow release function on the loaded growth factor, has a slow release period longer than 20 days, maintains the activity of the growth factor and can be used for the restoration treatment of the disease defect tissue.

The method of detecting the bioactivity of recombinant human liver cell growth factor adopts the technological scheme of applying NIH3T3 fibrocyte strain and mitochondrion dehydrogenase activity method to detect the bioactivity of rhHGF product. The method is one simple, feasible, specific and sensitive rhHGF bioactivity detecting method.

The invention discloses a bacterial cellulose / biological ceramic composite membrane loaded with growth factors and a preparation method thereof. The composite membrane is based on bacterial cellulose membrane, and is loaded with growth factors on its surface in the form of gel deposition coating, and then It is obtained by coating the bioceramic membrane on its surface by electrospinning technology; both the bacterial cellulose membrane and the bioceramic membrane have a nanofiber structure, which is used as a tissue repair material and has sufficient contact space with the cell environment of the damaged tissue; at the same time, It has a good effect of maintaining the activity of growth factors, and can control the release rate of growth factors by controlling the degradation rate of the composite membrane, and has good application value in tissue repair such as promoting tissue division and cell regeneration.