36 results about "Growth factor activity" patented technology

The invention discloses a preparation method of hydrogel for cartilage repair and with tissue inductivity. The preparation method comprises the following main steps: A, polysaccharide or protein double bonding, B, hydrogel preparation, and C, adsorption of growth factors. According to the preparation method, double bonded natural polysaccharide/protein and methacrylamide dopamine which are completely degradable are used as the main components, and hydrogel adopting a double network structure can be formed through free radical polymerization of two materials of double bonded polysaccharide/protein and methacrylamide dopamine. Then hydrogel adsorbs biological activity growth factors through the dehydration and reswelling functions, and the growth factors are fixed into hydrogel throguh the dopamine component. The hydrogel obtained according to the preparation method is excellent in biological degradability and cell/tissue affinity, high in activity of the loaded growth factors and good in slow release performance, and can better induce cartilage regeneration and promote cartilage repair.

The invention discloses a growth factor-loading silk fibroin/collagen bracket material and a preparation method thereof. The material is prepared from the raw materials of collagens, growth factors, silk fibroin, polylysine (PLL) and heparin sodium. A loose layer fibroin/collagen film and a compact layer fibroin/collagen film form a double-layered structure, and growth factor-loading PLL-heparin sodium nanoparticles are compounded in the middle of the double-layered structure. The preparation method comprises the following steps: preparing the loose layer fibroin/collagen film and the compact layer fibroin/ collagen film; preparing the growth factor-loading nanoparticles, compounding the fibroin/ collagen film and the nanoparticles and the like. The growth factor-loading silk fibroin/collagen bracket material disclosed by the invention plays a directional differential slow release role on the loaded growth factors and can fully maintain the activity of the growth factors and effectively prolong the action time of the growth factors, so that the material is a good guided tissue regeneration material for prosthodontic treatment of impaired and damaged tissues.

Modulation of the of glucocorticoid inducible kinases to restore Connective tissue growth factor activity. Also disclosed are methods and compounds useful for the detection and treatment of fibroproliferative disorders.

This invention relates to a kind of protein that possesses erythropoietin activity and its preparation. This protein is derivatized through its amino acid sequence is substituted by one or more amino acid residue, absence or addition ,in the condition that remaining tertiary structure of pleckstrin homology structural domain to be invariant, it possesses erythropoietin activity. Technology project of this invention is that function of haemopoietin EPO is grafted on backbone of pleckstrin homology structural domain by using protein functional grafting method, progress mutant design, expression and depuration, and determine the binding capability of mutant and EPO acceptor and EPO activity by experiment. This invented protein can be used as EPO succedaneum, used for curing anaemia, chronic renal failure(CRF),HIV infected/ZDU curing patient, arthritis deformans, carcino-anaemia and any other symptoms that can be cured by using EPO.

It is an object of the invention to examine the minimum unit of the exhibition of the activity of insulin-like growth factor-I and find a pharmaceutical use thereof in the fields of ophthalmology and dermatology. A joint administration of a peptide containing the amino acid sequence represented by Ser-Ser-Ser-Arg as the minimum unit of the exhibition of the activity of insulin-like growth factor-I and a peptide containing the amino acid sequence represented by Phe-Gly-Leu-Met-NH₂ is effective for curing corneal disorders and can significantly promote the healing of skin wounds.

The invention relates to a collagen-based bilayer membrane material for directional release of growth factors and a manufacturing method of the collagen-based bilayer membrane material. The collagen-based bilayer membrane material is prepared from collagen, the growth factors, fibrinogen and thrombin; a dense layer collagen membrane and a loose layer collagen membrane are prepared, and the dense layer collagen membrane and the loose layer collagen membrane are bonded by virtue of fibrous protein, so that a bilayer membrane structure is constituted. The growth factors can be loaded on the surface of the membrane by a dosage of 1-10000ng of the growth factors per square centimeter; preferably, the growth factors are loaded on the bilayer membrane by a dosage of 30ng per square centimeter; and preferably, average thickness of the bilayer membrane is 1-5mm. The material has a bidirectional controlled-release effect on the loaded growth factors, so that directional release of the growth factors is achieved and the material can act on target tissues; and the material is applicable to restorative treatment of diseased and defective tissues. The growth factors, which are loaded on the bilayer collagen membrane prepared by the invention and have a targeted release effect, can be released to the target tissues in a directional mode and activity of the growth factors can be kept; and the bilayer membrane material can be used for guiding and inducing regeneration and repair of tissues.

The invention discloses a preparation method of injectable polyamino acid hydrogel for cartilage repair. The preparation method comprises the steps: preparing double-bond functionalized polyamino acid; adding a solution of a sulfydryl-terminated cross-linking agent to prepare polyamino acid hydrogel; and washing, dehydrating, and then transferring into a solution containing growth factors for swelling to obtain the product. The hydrogel prepared by the method is mild in reaction condition and has good biodegradability and cell or tissue affinity, the loaded growth factors are high in activityand good in slow release performance, cartilage regeneration can be well induced, and cartilage repair is promoted.

The invention discloses a growth factor-loaded micro-nano composite membrane. The micro-nano composite membrane is composed of a growth factor-loaded egg shell membrane, a protein membrane, a hydrophilic micro-nano fiber layer and a hydrophobic micro-nano fiber layer; the micro-nano composite membrane takes the egg shell membrane as a direct carrier of a growth factor, and meanwhile protein servesas a coating layer to be deposited on the surface of the growth factor-loaded egg shell membrane to form the protein membrane through salting out, and then the hydrophilic micro-nano fiber membrane and the hydrophobic micro-nano fiber membrane wrap the surface of the protein membrane in sequence through an electrostatic spinning technology; the protein membrane is obtained through salting-out sedimentation by adopting poultry egg white. The micro-nano composite membrane is reasonable in structural design, so that the loaded growth factor has the slow-release property, and the materials forming the membrane layers have the effect of keeping the growth factor activity.

The invention discloses a growth factor loaded bacterial cellulose/biological ceramic composite membrane and a preparation method thereof. According to the composite membrane, a bacterial cellulose membrane is used as a basal body, growth factors are loaded on the surface of the bacterial cellulose membrane in a gel depositing and coating manner, and then a biological ceramic membrane is coated onto the surface through an electrostatic spinning technique so that the growth factor loaded bacterial cellulose/biological ceramic composite membrane is obtained. The bacterial cellulose membrane andthe biological ceramic membrane both have a nanometer fiber structure, so that the growth factor loaded bacterial cellulose/biological ceramic composite membrane can be used for materials for tissue repair, and has ample contact space with the cell environment of damaged tissue. Besides, the growth factor loaded bacterial cellulose/biological ceramic composite membrane has favorable effect of maintaining activity of the growth factors, the releasing speed of the growth factor can be controlled through controlling the degradation speed of the composite membrane, and the growth factor loaded bacterial cellulose/biological ceramic composite membrane has favorable application value in the tissue repair respect of promoting tissue division and cell regeneration and the like.

The method of detecting the bioactivity of recombinant human liver cell growth factor adopts the technological scheme of applying NIH3T3 fibrocyte strain and mitochondrion dehydrogenase activity method to detect the bioactivity of rhHGF product. The method is one simple, feasible, specific and sensitive rhHGF bioactivity detecting method.

The invention discloses a composition for preserving epidermal growth factor activity for a long time. The composition comprises a preservative, a protective agent and a phosphate buffer solution and preferably comprises the following components in percentage by mass: 0.00001-0.05% of EGF, 0.001-5.00% of the preservative and 0.01-25.00% of the protective agent and 0.01-0.1mol/L phosphate buffer solution with a pH value of 6-8 is used as the solvent. The preservative mainly comprises phenoxyethanol, DMDM hydantoin, benzyl alcohol, benzoic acid, methylisothiazolinone, propyl hydroxybenzoate and methylparaben; the protective agent mainly comprises hydrolyzed collagen, marine collagen, hydrolyzed wheat protein, sodium hyaluronate, amino acid, dextran and trehalose, ascorbic acid, hydrolyzed wheat protein, sodium hyaluronate, amino acid, glucan, mycose, ascorbic acid, water soluble vitamin E, chitosan and silk peptide; experiments prove that the composition can effectively alleviate the degradation of EGF and keep the bioactivity of EGF.

The invention provides a cream containing a growth factor activity protection technology. The cream can keep activity for a long time to promote cell growth in skin tissues, and adopts the technical scheme that a keratinocyte growth factor KGF is added into the cream, and glycerol, glucan and carboxymethyl deacetylated chitin are added into the formula of the cream, and through the synergistic effect of the three components, the activity of the growth factor is not lost for a long time.

The invention relates to the biotechnological field, and concretely relates to a method for screening snake vNGF active polypeptides. The method comprises the following steps: constructing fusion proteins with sputa NGF (vNGF) as a template; constructing a plasmid expression system through pBluscriptII SK+ and pQE30; carrying out separation, identification and purification through a 6X histidine recovery purification technology to form a stimulant stock solution of the vNGF active polypeptides; and verifying the biological activity of prokaryotic expression vNGF beta fragments. According to the invention, the sputa NGF is adopted as the template to carry out the breakpoint analysis of a functional zone, a design scheme that different polypeptide stimulants with two histone labeled fusion proteins as research models are constructed through gene recombination with the sputa NGF as the template is provided, and the activities of the vNGF polypeptides are researched to screen the vNGF active polypeptides and provide molecular biological supports for the basic researches of the sputa NFG application.

A canine hepatocyte growth factor gene and a 15 base pairs-deleted canine hepatocyte growth factor gene. These genes encode the following protein (a) or (b): (a) a protein having an amino acid sequence shown in SEQ ID NO: 2 or 4; and (b) a protein having an amino acid sequence comprising a deletion, substitution or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 2 or 4, and having a canine hepatocyte growth factor activity. The canine hepatocyte growth factor and the 5 amino acids-deleted canine hepatocyte growth factor are useful for the treatment of liver diseases, kidney diseases, lung diseases, digestive diseases, cardiocirculatory diseases or cranial nerve diseases.