A micro-nano composite membrane loaded with growth factors

Abstract

The invention discloses a growth factor-loaded micro-nano composite membrane. The micro-nano composite membrane is composed of a growth factor-loaded egg shell membrane, a protein membrane, a hydrophilic micro-nano fiber layer and a hydrophobic micro-nano fiber layer; the micro-nano composite membrane takes the egg shell membrane as a direct carrier of a growth factor, and meanwhile protein servesas a coating layer to be deposited on the surface of the growth factor-loaded egg shell membrane to form the protein membrane through salting out, and then the hydrophilic micro-nano fiber membrane and the hydrophobic micro-nano fiber membrane wrap the surface of the protein membrane in sequence through an electrostatic spinning technology; the protein membrane is obtained through salting-out sedimentation by adopting poultry egg white. The micro-nano composite membrane is reasonable in structural design, so that the loaded growth factor has the slow-release property, and the materials forming the membrane layers have the effect of keeping the growth factor activity.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a growth factor-loaded micro-nano composite film and its preparation method and application. Background technique [0002] As a water-soluble protein secreted by cells, growth factors have biological effects such as inducing and stimulating cell proliferation and maintaining cell survival, and play an important role in promoting cell proliferation, tissue repair and regeneration. Materials loaded with growth factors are widely used in the field of tissue repair materials. Concern; however, when the growth factor is used directly, it will be inactivated due to the existing environmental factors, making it less stable, short-lived, and unable to achieve the expected biological effect. [0003] At present, growth factors are usually loaded with biomaterials to continuously release growth factors to tissue repair cells within a certain period of time to induce repair and regener...

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Problems solved by technology

[0002] As a water-soluble protein secreted by cells, growth factors have biological effects such as inducing and stimulating cell proliferation and maintaining cell survival, and play an important role in promoting cell proliferation, tissue repair and regeneration. Materials loaded with growth factors are widely used in the field of tissue repair materials. Concern; however, when the growth factor is used directly, it will be inactivated due to the existing environmental factors, making it less stable, short-lived, and unable to achieve the expected biological effect

[0003] At present, growth factors are usually loaded with biomaterials to continuously release growth factors to tissue repair cells within a certain period of time to induce repair and regeneration of damaged tissues. At present, biomaterials loaded with growth factors are mainly used to form hydrogel biopolymers. Material-based, the loading method includes direct mixing and coating, and embedding growth factors into the gel material through layer-by-layer self-assembly, electrospinning and other technologies, but usually water-soluble gel molecules have poor mechanical strength, and in order to improve growth Factor loading and slow-release behavior often require the addition of additional chemical reagents, including inorganic fillers to enhance mechanical strength, porogens, defoamers, organic solvents, etc., and the addition of chemical substances will inevitably affect the growth factor. Activity affects

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