Method for preparing Nabothí»s cyst containing manifold cell growth factors and use thereof

A technology for growth factors and capsules, which is applied to medical preparations containing active ingredients, medical preparations with non-active ingredients, capsule delivery, etc.

Inactive Publication Date: 2008-01-30
齐长有
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there are no reports of nano-sustained-release microcapsules containing multiple growth factors at home and abroad. The present invention has prepared nano-capsules containing multiple cell growth factors for the first time. The growth factor slow-release effect is good, the activity is high, and it can be stored stably for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Dissolve 1 g of chitosan with a degree of deacetylation of 90% and a rotational viscosity of 240 cp in 40 ml of 1% acetic acid solution to form a 2.5% chitosan solution, add 0.5 g of polyvinylpyrrolidone with a degree of polymerization of 10,000, and stir until dissolved. Add 3 times the volume of emulsifier Span80 into the above system, and stir at 1200rpm. Take 20ul of the three cell growth factors containing EGF\FGF\IGF, the ratio of EGF\FGF\IGF is 1:1:1, the total concentration is 10%, add it dropwise to the above emulsion, continue to stir for 50min, the stirring speed is 1200rpm, At the same time, phacoemulsification was performed with a power of 30W for 1 hour, and at this time, nanocapsules containing three growth factors, EGF\FGF\IGF, were formed. The prepared microsphere emulsion was further transferred to a dialysis bag, sealed and placed in pure water for dialysis. The water was changed every 3 hours. After 24 hours of dialysis, the oily solvent was removed...

Embodiment 2

[0036] Morphology and particle size distribution of nanocapsules containing various cell growth factors. The morphology of bFGF sustained-release microspheres was observed under scanning electron microscope. The particle size of the nanocapsule was measured with a Malvern laser particle size distribution tester, and the diameter distance of the nanocapsule was calculated according to the regulations of the 2000 edition of the "Chinese Pharmacopoeia": diameter distance=(D90-D10) / D50. The average diameter was 230nm, and the polydispersity coefficient was 1.05-1.08.

Embodiment 3

[0038] Determination of drug loading and encapsulation efficiency of nanocapsules containing various growth factors. The absorbance value (A value) of the contained growth factor standard solution was determined by enzyme-linked immunosorbent assay. The logarithmic linear regression was carried out with the standard concentration C of the A value, and the regression equation was established. Take an appropriate amount of the newly prepared nanocapsule liquid, and ultra-speed refrigerated centrifugation at 4°C and 40,000 r / min for 2 hours, and the supernatant after centrifugation is used to determine the A value by ELISA method. Obtain the free drug amount in the supernatant (i.e. the free drug amount in the liquid medium) according to the regression equation again, and substitute into the calculation formula of drug loading and encapsulation efficiency to obtain that the drug loading is 25.8%, and the encapsulation efficiency is 87.3%. .

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Abstract

The invention pertains to the sustained-release nano field. The invention discloses a bran-new preparation method of a capsule containing a variety of cell growth factors. The invention applies the nano-biotechnology during the preparation process, takes the chitosan which is modified by polyvinylpyrrolidone as the capsule materials and makes use of the phacoemulsification extraction method to wrap a variety of cell growth factors in the capsule, therefore, the obtained compound growth factor capsule has good sustained-release effect, high activity of the growth factor and can be preserved stably for a long time. The capsule containing a variety of cell growth factors prepared by the invention can be compounded with the biocompatibility hydro gel, so as to be used for treating the superficial refractory wounds, furthermore, the invention can be also used for wound repair of the viscera, which can greatly promote the wound healing.

Description

technical field [0001] The invention belongs to the field of nano sustained release, and discloses a novel preparation method of nanocapsules containing various cell growth factors and its application in the field of wound repair. Background technique [0002] Nanocapsules refer to microcapsules with a particle size between 10 and 1000nm, which has the advantages of both microcapsules and nanoparticles. It can protect the contained substances from the influence of the external environment. When used as a drug release system, it can also Controlled release of internally encapsulated drug. On the other hand, its small size leads to its obvious surface effect, etc., which is manifested in good material affinity. When used as a catalyst, it has high catalytic efficiency, easy absorption, good biocompatibility, easy absorption in vivo, and easy migration. Wait. [0003] Tissue regeneration is dependent on growth factors, and better development and utilization of growth factors ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/32A61K38/18A61P17/02
Inventor 齐长有邓桂荣
Owner 齐长有
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