The invention discloses a minimally invasive surgical instrument with a flexible wrist part. The minimally invasive surgical instrument comprises an operating handle, a wrist part drive device, a connecting conduit, the flexible wrist part and an end executor. During the surgery process, due to the arrangement of a pricking and clamping hole in the body surface of a patient, a doctor can hold the intelligent minimally invasive surgical instrument and perform the surgery in the body. With the minimally invasive surgical instrument, all the operations of the common minimally invasive surgical instrument, including the instrument end opening and closing movements, can be completed, and meanwhile, the three-degree-of-freedom movements of the instrument end, including the pitching movement, the swinging movement and the autoroatation movement, can be completed by controlling the operating handle and the wrist part drive device. With the adoption of the minimally invasive surgical instrument, the spatial multi-degree-of-freedom surgery movements can be output, the movements are flexible, the operating force is large, and therefore, the minimally invasive surgical instrument is particularly suitable for carrying out the minimally invasive surgery with complicated movements.