The invention relates to the technical field of rat
lung transplantation, and provides a rat
intubation-free
lung transplantation construction method, in an operation, a high-flow
oxygen inhalation mask wrapping the mouth and the
nose of a rat is used for conveying, heating and humidifying
oxygen, effective
oxygenation under autonomous
respiration of the rat is maintained,
tracheal intubation and
mechanical ventilation support are not needed, and the operation is simple and convenient. The method specifically comprises the following steps: step 1,
anesthesia and
body position fixation; step 2, non-invasive ventilation support; step 3, performing
surgical approach and
exposure; step 4, fine dissociation of the pulmonary door structure; step 5, implanting a graft; and 6, closing the chest and resuscitating.
Airway injury,
postoperative infection and
respirator-related
lung injury caused by traditional
tracheal intubation are thoroughly avoided,
oxygen saturation in a rat operation is kept in a
stable state through high-flow heating and humidifying
oxygen inhalation and left-side clinostatism protection on the opposite lung, the death rate in the operation is greatly reduced, the pulmonary alveoli structure after lung
transplantation is complete and free of
inflammation infiltration, and the lung transplantation effect is good. The accuracy of
research data is obviously improved, and the physiological status is better fit.