Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Injectable polyamino acid hydrogel for cartilage repair and preparation method of injectable polyamino acid hydrogel

A polyamino acid and cartilage repair technology, which is applied in the fields of pharmaceutical formula, medical science, and prostheses, can solve the problems of expensive growth factors, short half-life, and activity destruction, and solve the problems of sudden release and local excessive toxicity, activity Maintain a good, good growth and repair effect

Pending Publication Date: 2020-06-09
NANJING UNIV OF TECH
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, growth factors are expensive, have a short half-life, and are easily denatured. Dropping growth factors directly on the surface of the hydrogel or directly swelling the solution containing the factors with the hydrogel is prone to burst release, and may also cause toxicity and carcinogenicity due to local overdose
The problem of sudden release and local excess toxicity can be solved by covalently immobilizing the factor in the wild group in the hydrogel composition, but the activity of the factor will be destroyed by the covalent reaction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Injectable polyamino acid hydrogel for cartilage repair and preparation method of injectable polyamino acid hydrogel
  • Injectable polyamino acid hydrogel for cartilage repair and preparation method of injectable polyamino acid hydrogel
  • Injectable polyamino acid hydrogel for cartilage repair and preparation method of injectable polyamino acid hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) Double bond functionalization of polyglutamic acid:

[0052]Dissolving polyglutamic acid in deionized water to obtain a solution with a mass concentration of polyglutamic acid of 1%, then dripping glycidyl methacrylate of 0.5 times the molar amount of polyglutamic acid in the solution, and then the reaction solution The pH was adjusted to 4.5, and the reaction was stirred at 50°C for 6 hours. After the reaction solution was cooled, the reaction solution was diluted with water to twice the original volume, dialyzed for 5 days, and dried for 1 day to obtain double bond functionalized polyglutamic acid.

[0053] (2) Preparation of polyglutamic acid hydrogel:

[0054] The double-bond functionalized polyglutamic acid is dissolved in phosphate buffer solution to obtain a double-bond functionalized polyglutamic acid solution with a mass concentration of 5%, and then mixed with an aqueous solution of sodium tetraborate containing dithiothreitol, rapidly Vortex to mix. The...

Embodiment 2

[0058] (1) Double bond functionalization of polyglutamic acid:

[0059] Dissolving polyglutamic acid in deionized water to obtain a solution with a mass concentration of polyglutamic acid of 0.1%, then dripping glycidyl methacrylate of 1 times the molar amount of polyglutamic acid in the solution, and then the reaction solution Adjust the pH to 3.5, stir and react at 30°C for 8 hours. After the reaction solution is cooled, dilute the reaction solution with water to 4 times the original volume, dialyze for 1 day, and dry for 1 day to obtain double-bond functionalized polyglutamic acid.

[0060] (2) Preparation of polyglutamic acid hydrogel:

[0061] The double-bond functionalized polyglutamic acid is dissolved in phosphate buffer solution to obtain a double-bond functionalized polyglutamic acid solution with a mass concentration of 0.5%, and then mixed with an aqueous solution of sodium tetraborate containing dithiothreitol, rapidly Vortex to mix. Then let it stand, and it wi...

Embodiment 3

[0065] (1) Double bond functionalization of polyglutamic acid:

[0066] Dissolving polyglutamic acid in deionized water to obtain a solution with a mass concentration of polyglutamic acid of 3%, then dripping glycidyl methacrylate of 5 times the molar amount of polyglutamic acid in the solution, then the reaction solution The pH was adjusted to 5, and the reaction was stirred at 4°C for 12 hours. After the reaction solution was cooled, the reaction solution was diluted with water to 10 times the original volume, dialyzed for 7 days, and dried for 3 days to obtain double bond functionalized polyglutamic acid.

[0067] (2) Preparation of polyglutamic acid hydrogel:

[0068] The double-bond functionalized polyglutamic acid was dissolved in phosphate buffer solution to obtain a double-bond functionalized polyglutamic acid solution with a mass concentration of 10%, and then mixed with sodium tetraborate aqueous solution containing dithiothreitol, rapidly Vortex to mix. Then let i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of injectable polyamino acid hydrogel for cartilage repair. The preparation method comprises the steps: preparing double-bond functionalized polyamino acid; adding a solution of a sulfydryl-terminated cross-linking agent to prepare polyamino acid hydrogel; and washing, dehydrating, and then transferring into a solution containing growth factors for swelling to obtain the product. The hydrogel prepared by the method is mild in reaction condition and has good biodegradability and cell or tissue affinity, the loaded growth factors are high in activityand good in slow release performance, cartilage regeneration can be well induced, and cartilage repair is promoted.

Description

technical field [0001] The invention belongs to the field of biological tissue engineering, and in particular relates to an injectable polyamino acid hydrogel for cartilage repair and a preparation method thereof. Background technique [0002] Hydrogel is composed of a three-dimensional network formed by the cross-linking of hydrophilic polymers, which can absorb and retain large amounts of water or biological fluids, and has similar physicochemical properties to extracellular matrix (ECM). In the fields of tissue engineering and regenerative medicine, injectable hydrogels have a porous three-dimensional network structure suitable for cell migration and proliferation due to their high water content, similarity to natural extracellular matrix, minimally invasive injection operations, and high With the advantages of matching any defect site, it has been widely used as a scaffold for cell growth and a carrier of various bioactive molecules. In recent years, a variety of biomat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08J7/02C08J3/075C08J3/24C08L77/04C08K5/37C08K3/38C08G69/48A61L27/18A61L27/50A61L27/52
CPCC08J7/02C08J3/075C08J3/24C08G69/48A61L27/18A61L27/50A61L27/52A61L27/58C08J2377/04C08K5/37C08K2003/387A61L2430/06A61L2400/06C08L77/04
Inventor 迟波刘帅杨荣
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com