4 results about "Cobra venom" patented technology

The invention relates to the technical field of biological medicines, and relates to specific primers and a method for identifying coral snake, cobra, king cobra and snake venom source species thereof, and application of the specific primers and the method. Based on highly-conservative and specific DNA fragments contained in DNAs extracted from tissues and snake venom of bungarus multicinctus, cobra and king cobra, specific primers are respectively designed for the fragments, and the bungarus multicinctus, cobra and king cobra and snake venom source species can be accurately identified. The method can be used for identifying bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus The method is high in detection sensitivity, high in correct detection rate, free of cross reaction and simple in operation process, and has higher specificity compared with a traditional immunoassay method.

The invention discloses an electrochemical sensor based on TdT enzyme and CbAgo synergistic mediation and application of the electrochemical sensor in cobra venom protein detection, and belongs to the technical field of electrochemical biosensors. Through magnetic bead enrichment, CbAgo protein cleavage and TdT extension reaction, and construction of a TdT enzyme and CbAgo protein synergistically mediated protein-nucleic acid conversion technology, a detection target is converted from cobra venom protein into a nucleic acid product, then the nucleic acid product is utilized to activate a Cas12a system to cut a probe P combined with a gold electrode, finally, the probe P is converted into an electrochemical signal, and the electrochemical signal is detected. The efficient and accurate detection of the cobra venom protein is realized. The electrochemical biosensor disclosed by the invention is high in sensitivity and good in specificity, and has extremely high application value in the aspects of accurate identification and quantitative analysis of cobra venom protein.

The application discloses an antibody or antigen binding fragment against agkistrodon antivenom and application thereof. The antibody or antigen binding fragment against agkistrodon antivenom comprises three heavy chain complementarity determining regions which can specifically bind to agkistrodon antivenom and the amino acid sequences of which are shown in SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8; and three light chain complementarity determining regions which the amino acid sequences of which are shown in SEQ ID NO:12, SEQ ID NO:14 and SEQ ID NO:16. The antibody or antigen binding fragment against agkistrodon antivenom can specifically bind to agkistrodon antivenom and does not bind to five-step snake venom, vipers venom, cobra venom and silver ring snake venom, and can be used for detection of agkistrodon antivenom. The monoclonal antibody of the application has neutralization and protection effects on agkistrodon antivenom, and has important significance for assisting clinical treatment, reducing complications and improving survival rate of patients.

The application discloses an electrochemical sensor based on TdT enzyme and CbAgo synergistically mediated and application thereof in detection of cobra venom protein, and belongs to the technical field of electrochemical biosensors.The application constructs a protein-nucleic acid conversion technology mediated by TdT enzyme and CbAgo protein through magnetic bead enrichment, CbAgo protein cleavage and TdT extension reaction, converts the detection target from cobra venom protein into a nucleic acid product, and then utilizes the nucleic acid product to activate a Cas12a system to cleave a probe P combined on a gold electrode, and finally converts into an electrochemical signal, so that efficient and accurate detection of the cobra venom protein is realized.The electrochemical biosensor has high sensitivity and good specificity, and has extremely high application value in accurate identification and quantitative analysis of the cobra venom protein.