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Application of cobra venom or extract thereof to preparation of medicament for reducing uric acid and/or resisting to gouty arthritis

A technology for gouty arthritis and cobra snake venom, applied in the field of medicine, can solve problems such as gouty arthritis of cobra snake venom that is not yet clear, and achieve the effects of reducing inflammatory cell infiltration, repairing joint damage, and improving thickening.

Active Publication Date: 2020-11-17
SUZHOU RENBEN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is not clear whether cobra snake venom is suitable for the treatment of hyperuricemia and gouty arthritis

Method used

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  • Application of cobra venom or extract thereof to preparation of medicament for reducing uric acid and/or resisting to gouty arthritis
  • Application of cobra venom or extract thereof to preparation of medicament for reducing uric acid and/or resisting to gouty arthritis
  • Application of cobra venom or extract thereof to preparation of medicament for reducing uric acid and/or resisting to gouty arthritis

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0029] Experimental Example 1 Animal Pharmacodynamic Experiment

[0030] 1. Experimental Materials and Methods

[0031] 1.1 Drugs and Reagents

[0032] Chinese cobra snake venom (NNAV) lyophilized powder was purchased from Ludongshan Snake Farm in Baojing, Hunan. The protein content is more than 90%, the ash content is less than 2%, and the neurotoxin content is 6.2% by HPLC.

[0033] Uric acid: purchased from Anhui Kuer Bioengineering Co., Ltd.; Uric acid determination kit: purchased from Nanjing Jiancheng Bioengineering Institute, batch number 20171025.

[0034] The preparation method of microcrystalline sodium urate (MSU): take 1g of uric acid, add 194ml of distilled water and 1mol / L NaOH 6ml, boil to dissolve the uric acid completely, after cooling in a water bath, drop in dilute HCl to pH 7.0, the solution is milky white, refrigerate at 4°C Place overnight, filter, dry at 50°C, save, and prepare for immediate use.

[0035] The preparation method of MSU suspension: MSU...

experiment example 2

[0063] Experimental Example 2 Animal Pharmacodynamic Experiment

[0064] 1. Experimental Materials and Methods

[0065] 1.1 Drugs and Reagents

[0066] Chinese cobra venom (NNAV): the same as the experimental example 1.

[0067] Uric acid: purchased from Anhui Cool Bioengineering Co., Ltd.

[0068] Microcrystalline sodium urate (MSU): take 1 g of uric acid, add 194 ml of distilled water and 1 mol / L NaOH 6 ml, boil to dissolve the uric acid completely, after cooling in a water bath, drop in dilute HCl to pH 7.0, the solution is milky white, put in a refrigerator at 4°C overnight, Filter, dry at 50°C, save, and prepare for immediate use.

[0069] The preparation method of MSU suspension: MSU is added to 0.9% sodium chloride solution to obtain MSU suspension with a concentration of 20 mg / ml.

[0070] 1.2 Experimental instruments

[0071] Electronic digital caliper: purchased from Shanghai Tool Factory Co., Ltd.; Microplate reader: purchased from Thermo Fisher.

[0072] 1.3 ...

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Abstract

The invention relates to the field of medicine, and particularly provides application of cobra venom or an extract thereof to preparation of a medicament for reducing uric acid and / or resisting to gouty arthritis. According to the invention, the cobra venom can effectively reduce the blood uric acid level of a hyperuricemic mouse, meanwhile, has an obvious improvement effect on the gouty arthritis, and can repair the joint injury caused by sodium urate deposition, reduce the joint swelling rate, improve the joint synovial tissue thickening condition, reduce inflammatory cell infiltration, protect cartilage cells; and clinical experiments show that cobra neurotoxin can quickly eliminate subcutaneous gout calculi of gout patients, lumps disappear after the cobra venom is injected into the patients for three days, and no side effect exists. In conclusion, the Chinese cobra venom has the effect of repairing gouty arthritis while reducing uric acid, and can be used as a medicament for treating gout.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of a cobra venom or an extract thereof in the preparation of a medicine for lowering uric acid and / or anti-gouty arthritis. Background technique [0002] Uric acid is the end product of purine metabolism in humans. Due to the lack of uric acid oxidase in the human body, humans are prone to form hyperuricemia. Hyperuricemia is a metabolic disorder caused by purine metabolism disorders that increase uric acid production or decrease excretion. Disease, according to statistics, about 5%-12% of hyperuricemia will eventually develop into gout. If hyperuricemia persists for a long time, uric acid will be deposited in joints, subcutaneous tissues and kidneys in the form of urate, causing a series of clinical diseases such as gouty arthritis, subcutaneous gout stones, kidney stones or gouty nephropathy. kidney failure, life-threatening. Long-term hyperuricemia leads to an increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/583A61P19/06
CPCA61K35/583A61P19/06
Inventor 秦正红
Owner SUZHOU RENBEN PHARMA
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