50 results about "Chinese cobra" patented technology

The present invention relates to the identification of the active domain of Herpoxin, a DNA virus-inhibiting-protein which was isolated from cobra venom in U.S. Pat. No. 5,648,339 and has a molecular weight of 13.5 kDa We have isolated a fragment of Herpoxin which contains the active domain and which we have named Herp. Herp mimics the activity of Herpoxin in inhibiting the replication of DNA viruses. A synthetic version of the active fragment was produced having the amino acid sequence Asn-Leu-Tyr-Gln-Phe-Lys-Asn-Met-Ile-Gln. The synthetic version of Herp consisting of ten amino acids inhibits the replication of DNA viruses such as herpes viruses types 1 and 2, cytomegalovirus and varicella zoster virus as well as Tubercle bacilli.

The present invention discloses the tumor invasion and metastasis resisting function and application of venin cysteine proteinase inhibitor, and belongs to the field of biomedicine. Through designing and synthesizing sv-Cystatin cDNA according to 99 amino acid sequences of Chinese cobra venin Cystatin protein, cloning to pPICZ alphaA vector and transforming Pichia yeast, stable and high expression engineering bacterium GS115-sv-Cystatin is screened out. Through further inducing expression and purification, the extracorporeal bioactivity experiment shows that the recombinant sv-Cystatin protein has the functions of inhibiting the activity of papain and inhibiting the tumor cell invasion and metastasis obviously, and so do the intracorporeal experiment. The present invention also constitutes pcDNA-sv-Cystatin eukaryotic expression vector. The present invention has huge application foreground in preventing and treating tumor.

The invention provides a method for separating and purifying cobra neurotoxin protein through dual-ion exchange chromatography. The method comprises the following steps of: (1) first separation and purification through ion exchange chromatography, namely, a) dissolution of crude cobra venom, and b) separation and purification through an SP-Sephodex-C25 gel column; and (2) second separation and purification through ion exchange chromatography, namely, a) balance of a SourceS(XK5030) column having a column volume of 500 ml by using 1000 ml of liquid A for future use; and b) SourceS(XK5030) column purification, and then collection of the cobra neurotoxin protein purified twice according to a standard purification chromatogram. The method provided by the invention is scientific and rational in process; and no organic reagent, no high-concentration salt and no protein modification method are used in production, so that the biological activity of cobratide can be maintained to an utmost extent. The method provided by the invention is advanced in process and simple to operate; the production period can be greatly shortened, the production time can be saved and the production cost can be reduced; and the method is completely suitable for an industrial production line.

The invention discloses an application of cobrotoxin in preparing a medicine for treating arthritis. Cobrotoxin is a neurotoxin purified from Chinese cobra-venom. People research discovers that the cobrotoxin in the range of 1-600mug / kg has obvious effect on treating rheumatoid arthritis, can relieve the symptoms of arthrocele, arthralgia and the like, and has the advantages of small dose, safety,capability of oral administration, injectability, administration through oral mucous membrane, nasal mucous membrane and skin, and the like.

The invention discloses an application of physically-modified Chinese cobra venom in preparation of medicaments for treating acute and chronic nephrosis. The physically-modified Chinese cobra venom is obtained by dissolving Chinese cobra venom with deionized water, heating for denaturation and then performing renaturation; the heating temperature is controlled to be 50-100 DEG C, and the heating time is controlled to be 1 min-300 min. The invention finds that the heated and denaturated Chinese cobra venom can effectively reduce urine protein in acute and chronic nephrosis, reduce serum creatinine, and urea nitrogen, improve the pathological damage degree of the kidney, and recover kidney functions.

The invention discloses application of cobra venom in preparation of a medicament for treating lung inflammatory disease and pulmonary fibrosis. Animal experiment proves that Chinese cobra venom can alleviate acute endotoxin poisoning symptom within a range of 10-3000mug / kg, particularly has obvious therapeutic action on lung inflammatory disease caused by endotoxin, and can be used for alleviating inflammatory cell infiltration, alveolar septum thickening, and focal atelectasis and emphysema. Repeated small dose of endotoxin can result in pulmonary fibrosis, the cobra venom can significantly alleviate pulmonary fibrosis symptom and reduce alveoli damage and collagen hyperplasia. Naturally-modified Chinese cobra venom and physically-modified Chinese cobra venom have similar actions, but the physically-modified Chinese cobra venom has better action. Used for treating lung inflammatory disease, the Chinese cobra venom has the advantages of low dose, safety, capability of being orally administrated, injected, and administered through oral mucous membrane and skin and the like.

The invention discloses snake gall tea and a cultivation method thereof. The cultivation method comprises the following steps: 1) extracting snake gall bile: selecting pollution-free, healthy and high-quality cobras, and extracting cobra gall bile and immediately and temporarily storing remained galls at 0-4 DEG C; 2) treating the galls: crushing the galls obtained by the step 1) and adding pure water, putting a mixture into a steam blasting pot, wherein the pressure is 4MPa, heating to 180 DEG C and keeping for 3min, and rapidly releasing pressure within 1s-3s to obtain slurry; and 3) dissolving the slurry obtained by the step 2) into water to prepare a mother solution, diluting and adding water in a utilization process and uniformly spraying a solution to tea leaves of tea trees, and after spraying for 15 days, harvesting to obtain the snake gall tea. The snake gall tea contains more active components and no parasites.

The invention discloses wulong wind-dispelling dampness-removing health snake wine and a preparation method thereof. Every 50 kg of liquor is added with the following components: long-noded pit viper, black snake, cobra, rhabdophis tigrinus and dinodon rufozonatum and traditional Chinese medicines comprising cortex acanthopanacis, Chinese angelica, astragalus, Sichuan lovage rhizome, notopterygium root, angelica pubescens, eucommia ulmoides, radix cyathulae, flowers carthami, cassia twig, homalomena occulta, large-leaved gentian, pawpaw, radix clematidis, codonopsis pilosula, teasel root, berba aristolochiae mollissimae, earthworm and radix glycyrrhizae. The preparation method comprises the following steps: steaming the snakes and adding the steamed snakes and the traditional Chinese medicines into wine; sealing a cylinder end and placing the cylinder in a shade place for 1-3 years, and keeping the temperature lower than 25 DEG C; opening the cylinder and filtering to obtain filtrate, and adding 5% (in proportion by weight) of honey into the obtained filtrate; sealing the filtrate and placing the filtrate in an environment with a temperature lower than 15 DEG C for 5-7 days, getting liquid supernatant and filling. The snake wine prepared by the preparation method disclosed by the invention is golden in color, has honey aroma, and is slightly bitter, and extremely good in mouthfeel. The snake wine has effects of promoting blood circulation to remove blood stasis, dispelling wind and removing dampness, and relieving pains, and especially has a remarkable curative effect for diseases caused by rheumatoid.

A modified human complement C3 protein (C3) is disclosed comprising a substitution of a portion of a human C3 protein, with a corresponding portion of a Cobra Venom Factor protein (CVF) which results in a human C3 protein with CVF functions, but with substantially reduced immunogenicity. Advantageously, the C3 protein can be manipulated to contain at least one of the following CVF functions: increased stability of the C3 convertase and increased resistance to the actions of factors H and / or I. A large number of hybrid C3 proteins containing substitutions in the C-terminal portion of the alpha chain of C3 are presented and tested for the above functions. Methods of treatment of diseases such as reperfusion injury, autoimmune diseases, and other diseases of increased complement activation are presented as well as methods of increasing the effectiveness of gene therapeutics and other therapeutics.

The invention discloses a purification preparation method of cobra venom CTX (cytotoxin)-4N. According to the method, a DEAE-SepharoseCL-6B anion exchange column is firstly used; then, a SephadexG-50 gel chromatographic column and a Macro-prep High S cation exchange column are used for respective separation; after separation in each time, the peak, with the highest activity, of the cobra venom CTX-4N is selected as a sample of the subsequent separation. Research shows after the method is used, CTX-4N with electrophoresis purity can be obtained; the purity is high; the activity is high. HSC-T6 proliferation inhibition and apoptosis experiments prove that the CTX-4N has a killing effect on HSC-T6 cells; the condition that the CTX-4N has the anti-hepatic fibrosis effect in the hepatic fibrosis development process is prompted; important medicinal values are realized.

The invention relates to the field of medicine, and particularly provides application of cobra venom or an extract thereof to preparation of a medicament for reducing uric acid and / or resisting to gouty arthritis. According to the invention, the cobra venom can effectively reduce the blood uric acid level of a hyperuricemic mouse, meanwhile, has an obvious improvement effect on the gouty arthritis, and can repair the joint injury caused by sodium urate deposition, reduce the joint swelling rate, improve the joint synovial tissue thickening condition, reduce inflammatory cell infiltration, protect cartilage cells; and clinical experiments show that cobra neurotoxin can quickly eliminate subcutaneous gout calculi of gout patients, lumps disappear after the cobra venom is injected into the patients for three days, and no side effect exists. In conclusion, the Chinese cobra venom has the effect of repairing gouty arthritis while reducing uric acid, and can be used as a medicament for treating gout.

The invention relates to application of a postsynaptic neurotoxin, a cardiotoxin, a cytotoxin, a phospholipase A2 and a crude venom of a cobra in resisting virus infection. A respiratory tract is easily invaded and infected by viruses due to an open anatomical structure to the outside, and viral influenza and pneumonia are relatively common cases in daily life; and in addition, hepatitis B virusesand AIDS viruses can infect human beings to cause diseases through various ways. The current treatment means of respiratory diseases caused by viruses is not ideal in vaccine or drug effect, and thetreatment effect of viral hepatitis and AIDS needs to be further improved, so that a product which has broad-spectrum inhibition capacity on viruses and has small side effect is developed to become aclinical urgent need. The postsynaptic neurotoxin, the cardiotoxin, the cytotoxin, the phospholipase A2 and the crude venom of the cobra show the effectiveness in resisting virus infection, and possibly become a candidate of a new antiviral product.

The invention discloses Dong nationality medicinal liquor. The Dong nationality medicinal liquor is prepared from the following raw materials in parts by weight of 15-25g of Chinese wolfberry fruits,30-40g of rhizoma polygonati, 20-30g of radix codonopsis, 30-40g of fleeceflower roots, 10-20g of longans, 25-35g of radix rehmanniae preparata, 10-20g of radix angelica sinensis, 15-25g of poria cocos, 15-25g of radix ophiopogonis, 25-35g of radix rehmanniae, 10-20g of rhizoma drynariae, 35-45g of cortex eucommiae, 10-20g of rhizoma alismatis, 15-25g of radix polygalae, 10-20g of radix astragali,25-35g of herba cistanches, 15-25g of morinda roots, 15-25g of spina date seeds, 15-25g of Chinese yams, 25-35g of dateplum persimmons, 15-25g of red jujubes, 10-20g of Chinese magnoliavine fruits, 45-55g of crystal sugar, 450-550g of cobras, 350-450g of long-noded pit vipers, 350-450g of pallas pit vipers and 550-650g of ophidia black snakes. Through reasonable compatibility of traditional Chinese medicines, the Dong nationality medicinal liquor has notable treatment effects on multiple diseases.

The invention relates to the technical field of special animal breeding, and discloses a cobra breeding box. The cobra breeding box comprises a breeding box body, a material taking opening is formed in the top of the breeding box body, a material taking baffle is movably arranged in the material taking opening through a hinge, a drinking water box is arranged on the left side of the inner bottom surface of the breeding box body, a rectangular drinking water trough is formed in the top of the drinking water box, two heat conduction blocks are fixedly arranged on the right side of the drinking water box, a temperature conduction pipe is fixedly arranged between the two heat conduction blocks, and two inlet grooves are symmetrically formed in the right side of the top of the drinking water box and are both semi-cylindrical grooves. According to the cobra breeding box, recycling holes and stop sheets are arranged, when cobras pass back and forth through the inlet grooves, after dust and water on the surfaces of the cobras are cleared through a clearing device, the dust and the water falling into the inlet grooves fall downwards into the recycling holes along the arc-shaped inner wall surfaces of the inlet grooves, part of the water and the dust falling into the recycling holes are recycled into the drinking water trough along inner channels of the recycling holes, and the effect ofrecycling the water collected in the inlet grooves is achieved.

The invention discloses Lingshan snake bile tea and a processing method thereof. The processing method comprises the following steps: 1) selecting a snake bile source: selecting healthy, strong and high-quality snakes with long artificial culture time from a pollution-free snake farm; 2) extracting snake gallbladders: after killing the snakes, washing off blood, and immersing the snake gallbladders in wine for cleaning; putting the snake gallbladders into a container and soaking with wine for later use; 3) treating the snake gallbladders: (1) taking out the snake gallbladders soaked in the step 2) and piercing to press out bile; filtering; (2) adding pure water into the bile obtained by the step (1) and uniformly mixing; (3) sterilizing snake bile water obtained by the step (2) through utilizing high-temperature steam in a sealed manner, and adding the snake bile into a high-pressure homogenizing machine for homogenizing; (4) uniformly mixing the treated snake bile of cobras and coral snakes in the step (3) to prepare snake bile mother liquid for later use; (5) diluting the snake bile mother liquid obtained by the step (4) with pure water for later use; 4) mixing snake bile liquid with finished-product tea: spraying the snake bile liquid obtained by the step 3) on the finished-product tea in a mist form, and overturning to enable the finished-product tea to sufficiently absorb the snake bile liquid, and baking to obtain the snake bile tea.

The invention discloses a method for purifying cobra venom and its products. The invention provides a method for purifying cobra venom, which comprises the following steps: (1) performing cation exchange chromatography on a phosphate buffer containing the cobra venom to obtain an eluent A containing an active ingredient; wherein the phosphate buffer containing the cobra venom includes the cobra venom and a phosphate buffer solution; (2) performing complex mode chromatography on the eluent A containing the active ingredient to obtain an eluent B containing the active ingredient; and (3) desalting the eluent B containing the active ingredient to obtain the active ingredient. The purifying method has the advantages of simple process, easy industrialization, and low cost, and obtained producthas the advantages of high purity and low content of related substances.

The invention relates to the technical field of traditional Chinese medicines, in particular to an external medicinal liquor for treating soreness, swelling, numbness and pain of body joints and a preparation method thereof, which is characterized in that the external medicinal liquor comprises the following materials in parts by weight: 20-30 parts of cobra, 25-30 parts of garter snake, 20-40 parts of monkshood, 20-40 parts of radix aconiti agrestis, 20-35 parts of artemisia rupestris, 20-38 parts of semen strychni, 20-35 parts of ligusticum wallichii, 15-20 parts of radix curcumae, 20-35 parts of flowers carthami, 200-30 parts of garden balsam stem, 20-30 parts of dried rehamnnia root, and the balance of liquor. The traditional Chinese medicine wine is an external traditional Chinese medicine wine, which is specifically a traditional Chinese medicine formula for treating various arthralgia, neuropathic pain, traumatic injury and numbness of joints caused by qi and blood stasis of a human body due to rheumatism and rheumatoid, and can effectively solve the medication problem of various diseases of the body joints.

The invention discloses a processing method for a medicinal raw material of an artificially bred cobra. The processing method comprises the following steps of (1) collecting the cold-chain transportedcobra by using a thickened nylon mesh bag; (2) performing weighing in a factory, and randomly sampling and measuring medicine residues; (3) putting the cobra into a retention chamber for low-temperature retention until the cobra enters a hibernation state; (4) pouring the cobra into a normal-temperature pool, and performing immersion cleaning for 20-30 seconds; (5) putting the cobra into a cold water pool, and performing rinsing for 1-2 minutes; (6) fixing the head of the cobra without extruding the poison gland, fixing the tail of the cobra to prevent curling and struggling, obliquely putting a funnel into the mouth to receive venom, and performing electrode discharge to promote venoming; (7) after venoming is finished, cutting the head to collect the head of the cobra, and carrying outbloodletting to collect blood; (8) feeding the cobra body into a hot water pool for soaking, removing scales, and spray-washing the surface of the cobra body; (9) cutting open the abdomen of the cobrabody to strip the internal organs, and cleaning the cobra body; and (10) centrifugally spin-drying water on the surface of the cobra body, and performing disinfection, weighing and packaging in vacuum to obtain the finished fresh cobra body. The method can be suitable for processing cobras into medicinal raw materials on a large scale, and can reduce or avoid accidents that people are bitten by the cobras.

The invention discloses a preparation method of cobra traditional Chinese medicine raw powder, and belongs to the technical field of medicine powder processing, and the preparation method comprises the following steps: crushing: taking cobra decoction pieces, drying until the handfeel is dry, then putting into crushing equipment for crushing, and then putting into superfine crushing equipment for continuously crushing to obtain fine powder; s2, sterilization: sterilizing the fine powder obtained in the step S1 by adopting an irradiation sterilization method to obtain sterilized fine powder; and packaging: packaging the fine powder treated in the step S2 with a medicinal composite film to obtain a finished cobra powder product. The cobra traditional Chinese medicine raw powder fine powder prepared by the method is high in powder yield and complete in effective components, and the microbial limit of the cobra traditional Chinese medicine raw powder fine powder meets the microbial limit standard of traditional Chinese medicine decoction pieces in non-sterile product microbial limit inspection in the general rule of Chinese pharmacopoeia in 2020 edition 4; the water content and the total ash content conform to the second method 0832 of the four-part general rule of the Chinese pharmacopoeia 2020 edition and the provision 2302 of the four-part general rule of the Chinese pharmacopoeia 2020 edition.

The invention discloses a high-titer anti-cobra neurotoxin serum and a preparation method thereof. The preparation method comprises the following steps: firstly, reducing neurotoxicity of cobra venom; then, embedding the cobra venom in polyacrylamide gel; and finally, grinding neurotoxin-containing gel, performing immune injection on rabbits to obtain antiserum, and performing cryopreservation. In the invention, different doses of cobra venom are adopted; the neurotoxicity is reduced by heating, SDS and beta-mercaptoethanol treatment; the emulsification is replaced with polyacrylamide gel embedding, so as to ensure healthy survival of immunized animals, reduce production cost of he antiserum, and prepare high-titer antiserum.

The invention relates to the technical field of cobra breeding, and discloses a cobra breeding-based breeding box. The breeding box comprises a main body; the main body is a hollow box body composed of a triangle and a rectangle; a trapezoidal block is fixedly installed at the central point of the interior of the rectangle on the main body; inlets are formed in the front and rear side wall surfaces of a rectangular block on the main body; the inlets are rectangular through holes; openable door plates are movably clamped in the two inlets; the door plates are rectangular blocks; and the door plates are matched with the interiors of the inlets in shape and size. When in use, water can be injected into a partition plate through a water injection pipe, and the water is discharged into a waterstorage plate through two drainage holes in the partition plate to be collected, so that the environment humidity in the main body is ensured, the humidity in a shed is ensured to be 60%-80%, and it is avoided that due to the fact that the humidity in the main body does not reach the standard, the growth speed and survival rate of cobras are influenced and the breeding cost is increased.

The invention discloses an anticoagulant drug based on cobra venom PIII type metalloprotease. The cobra venom PIII type metalloprotease is extracted from snake venom of naja atra and then purified. The metalloprotease is single chain glycoprotein. The structure analysis shows that metalloprotease has three structural domains: metalloprotease structural domain, disintegrin like structural domain, and cysteine enriched structural domain, and belongs to PIII type cobra venom metalloprotease. The metalloprotease is named as Atrase A and Atrase B; and the sequences of Atrase A and Atrase B are represented by SEQ ID No.1 and SEQ ID No.2 respectively. Target protein and anticoagulant drugs thereof have multiple anticoagulant effects: preventing multiple blood coagulation factors; reducing fibrinogen level; preventing platelet aggregation; and preventing complement so as to relieve abnormal blood coagulation caused by inflammation.

The invention relates to an application of a cobratide preparation in preparation of a medicine for treating haemorrhoids. The cobratide which is a main component of the cobratide preparation is neurotoxin protein separated and purified from cobra venom, and the content of the neurotoxin protein is not less than 35.0 percent according to a dry product; the preparation is an injection, an oral preparation, an enteric preparation, a rectal administration preparation, an oral absorption preparation and the like. The cobratide preparation has a good effect on main symptoms such as hematochezia, prolapse, anal pendant expansion, pain, anal foreign body sensation and the like represented by haemorrhoids. In addition, animal experiments prove that the preparation provided by the invention has thecurative effect of treating hemorrhoid-related diseases, and provides another choice for patients.

The invention discloses a manufacturing method of a Chinese cobra bite pig model and half lethal dose (LD50) of cobra venom to pigs and application of the cobra venom to an animal model. Cobra venom freeze-dried powder is dissolved and then injected into pig leg muscles according to 2mg / kg, and a target animal model can be obtained after 6 hours, so that the problem that no Chinese cobra bite model of large animals in the prior art is solved. The model has the advantages of high success rate, strong repeatability, stable pathological change and the like. The cobra bite disease condition is long, the model simulates human being bite by the cobra, the physiological and pathological dynamic process of the human being bite by the cobra can be well close to, and dynamic analysis of the disease clinical manifestation and mechanism of the cobra bite is facilitated. The model can provide a good animal model for researching metabolic change of Chinese cobra venom in a human body and a muscle necrosis mechanism, and provides an innovative technical method support for researching clinical characterization of cobra bite, pathophysiological mechanism, precise treatment, screening of anti-venomous snake bite medicines, evaluation of curative effect of anti-venom serum and the like.

The invention discloses application of long-chain neurotoxin (alpha-cobratoxin, alpha-CbTX) of cobra in preparation of a muscarine type M4 receptor activator. The neurotoxin has the function of reducing pain through activating M4 receptor-dependent T type calcium current, and the muscarine type M4 receptor activator containing the long-chain neurotoxin of the cobra can be established to perform the application of clinical analgesia.

The invention relates to the field of traditional Chinese medicine preparations for eliminating snake venom, in particular to a traditional Chinese medicine preparation for eliminating the snake venom and a preparation method thereof. An active ingredient of the traditional Chinese medicine preparation is prepared from, by weight, 1-100 parts of radix angelicae, 1-100 parts of radix astragali, 1-100 parts of radix glycyrrhizae, 1-100 parts of fructus forsythiae, 1-100 parts of bulbus fritillariae thunbergii, 1-100 parts of folium isatidis, 1-100 parts of rhizoma coptidis, 1-100 parts of fructus evodiae, 1-100 parts of radix clematidis, 1-100 parts of trogopterus dung, 1-100 parts of rheum officinale, 1-100 parts of ligusticum wallichii, 1-100 parts of herba asari and 0.5-50 parts of centipede. The traditional Chinese medicine preparation has the advantages that the preparation can effectively treat the snake venom of cobra or coral snake, can astringe to promote granulation, promote wound healing, can repair wounds by external application without scarring and is free of side effect.

The invention discloses a preparation method of a cobra venom nerve growth factor. The method is characterized in that crude cobra venom is subjected to gel chromatography twice, wherein the gel chromatography I is SP-Sephadex G-25 column chromatography, and the gel chromatography II is Acta Rosorse column chromatography. The cobra venom nerve growth factor provided by the invention is simple and feasible in preparation method, can be used for industrial large-scale production, and is small in activity loss, and the activity loss of the cobra venom NGF is less than 25%; the conventional limitation of protein separation can be broken through, and the crude snake venom liquid with higher initial concentration can also be used for effective separation; the method is high in production efficiency, reasonable in cost and particularly suitable for industrial large-scale production; and the yield is high, exceeds 0.5%, and even can reach 0.6% or above.