Venin-sourced demulcent CTXn, and purification method and applications thereof

A technology of cytotoxin and purification method, applied in the field of biopharmaceuticals, to achieve strong analgesic effect, significant analgesic effect, and obvious dose-effect relationship
CN101717441AInactive Publication Date: 2010-06-02广州医学院
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
广州医学院
Publication Date
2010-06-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a venin-sourced demulcent CTXn, and a purification method and applications thereof. The invention is characterized in that (1) the molecular weight of the CTXn is 6697.429 Da; (2) and the amino acid sequence of the CTXn is described as the sequence list SEQ ID No.1. The preparation method comprises the following steps of: using a Zhoushan cobra venom sample; carrying out the CTXn separation and purification on the Zhoushan cobra venom sample; primary-screening to select a component III for next step of ion exchange; carrying out the ion exchange on the component III through a quick-purification technique exploitation system by using a filler to obtain a single-virulence polypeptide CTXn, desalting with a G-50 prepacked column, and freeze-drying; and measuring the molecular weight and the amino acid sequence of the CTXn. The CTXn of the invention has favorable demulcent function on somatalgia and visceralgia caused by nociceptive stimulus as well as pains caused by addiction to morphine, has the characteristics of obvious drug effect and low toxic or side effect, is beneficial to large-scale production, and has wide application prospects in the aspect of analgesic development.
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Description

technical field

[0001] The invention relates to a cytotoxin CTXn derived from snake venom for analgesia, a purification method and application thereof. A cytotoxin derived from the poison of Zhoushan cobra (Naja atra Cantor) is mainly used, which is suitable for analgesia, especially involving the new pharmaceutical application of the cytotoxin for treating acute and chronic pain. It belongs to the technical field of biopharmaceuticals. Background technique

[0002] Since the 1970s, NT has conducted in-depth research on the basic research of analgesia, and its analgesic mechanism may be related to various systems such as cholinergic and endogenous opioid peptides. Compared with the traditional analgesic drug morphine, the analgesic effect of NT has the characteristics of less dosage, longer analgesic time and no addiction, etc. It is effective for intractable pain, neuropathic pain and malignant tumor pain. In view of the high neurotoxicity and lethal activity of NT, it is...

Claims

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