The invention discloses a preparation method and application of a bulbus fritillariae cirrhosae extract. The method comprises the following steps: pulverizing bulbus fritillariae cirrhosae, soaking the bulbus fritillariae cirrhosae in an alcoholic solution overnight, carrying out continuous reflux extraction at 60-90 DEG C, collecting an extracted concentrated solution, removing a solvent to obtain a concentrated extract, and carrying out reduced-pressure vacuum drying to obtain the bulbus fritillariae cirrhosae extract. Alkaloids in the bulbus fritillariae cirrhosae extract comprise sipeimine, peiminine, peimine, edpetiline, peimine, peimisine, sinpeinine A, yibeinoside A and ebeiedinone. The bulbus fritillariae cirrhosae extract disclosed by the invention can be used as a medicine for treating chronic respiratory system diseases, including chronic obstructive pneumonia, chronic pharyngitis and pulmonary fibrosis, and can activate MAPKs and Nrf2 signal channels.