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Annular gene-oriented paper micro-fluidic chip detection card for rapidly screening chronic obstructive pneumonia at early stage and test strip of detection card

A chronic obstructive, microfluidic chip technology, applied in the field of biomedicine, can solve the problems of being unsuitable for large-scale rapid screening, time-consuming, instrument accuracy, and site requirements, etc., to achieve fast detection results and short time-consuming. , The effect of low detection cost

Inactive Publication Date: 2019-08-09
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current gold standard for diagnosis - lung function test (forced expiratory volume in one second (FEV) 1 ) / forced vital capacity (FVC)<70%) have high requirements on instrument accuracy and venue, and take a long time, so it is not suitable for large-scale rapid screening in communities or factories.

Method used

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  • Annular gene-oriented paper micro-fluidic chip detection card for rapidly screening chronic obstructive pneumonia at early stage and test strip of detection card
  • Annular gene-oriented paper micro-fluidic chip detection card for rapidly screening chronic obstructive pneumonia at early stage and test strip of detection card
  • Annular gene-oriented paper micro-fluidic chip detection card for rapidly screening chronic obstructive pneumonia at early stage and test strip of detection card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A paper-based microfluidic chip detection card and its test strip for rapid screening of chronic obstructive pneumonia (hsa_circ_0005045 circular gene) for the early stage of the circular gene, including the upper card cover 1 and the lower card cover 2, and the lower card cover 2 An upper card cover 1 is provided on the top, and a test strip groove 3 is provided on the inner surface of the lower card cover 2, and a test strip 4 is arranged in the test strip groove 3; the test strip 4 includes a PVC bottom plate 5 And the sample pad 6 , the gold standard pad 7 , the nitrocellulose membrane 8 and the sampling pad 9 lapped on the PVC bottom plate 5 in sequence.

[0037] The inner surface of the upper card cover 1 is provided with fixing bars 10 around, and the inner surface of the lower card cover 2 is provided with cylindrical grooves 11 corresponding to the fixing bars 10 .

[0038] The outer surface of the upper card cover 1 is provided with an anti-slip hand-held groo...

Embodiment 2

[0041] This embodiment is to provide the preparation method of the test strip 4 described in embodiment 1.

[0042] 1. Preparation of hsa_circ_0005045 probe antibody and SA-labeled colloidal gold particles

[0043] Prepare colloidal gold by trisodium citrate reduction method: add 200ml of deionized water to the round bottom flask treated with soaking acid and silicification, heat it to boil with electric heating mantle, stir at 200r / min; quickly add 2ml of 1% chlorine Bring the gold acid solution to boiling; add 6ml of trisodium citrate solution, the solution will gradually turn into wine red until the color remains unchanged, then heat for 10min, then stop heating and stir for 15min; after returning to room temperature, restore the volume to 200ml, 4°C Store in the refrigerator.

[0044]The synthesized probe 1 and probe 2 (SEQ ID NO: 1 and SEQ ID NO: 2, purchased from thermo fisher company, each with a final concentration of 100 μM) were added to TCEP-HCl (final concentratio...

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Abstract

The invention relates to an annular gene-oriented paper micro-fluidic chip detection card for rapidly screening chronic obstructive pneumonia at an early stage and a test strip of the detection card.The detection card comprises an upper clamping cover and a lower clamping cover; the upper clamping cover is arranged on the lower clamping cover; a test strip groove is formed in the inner surface ofthe lower clamping cover; a test strip is arranged in the test strip groove; the test strip comprises a PVC bottom plate as well as a sample pad, a gold labeled pad, a nitrocellulose membrane and a sample absorption pad which are sequentially in lap joint with the PVC bottom plate. The test strip disclosed by the invention has the advantages of strong specificity and high sensitivity. According to the detection card and the test strip of the invention, widely applied and high-efficiency base complementation and compound binding reactions are adopted to perform detection, and therefore, the level of proteins in a blood sample can be accurately detected; and a high-quality gene probe and a compound are combined so as to realize capture, so that the sensitivity of the nucleic acid detectiontest strip is greatly improved.

Description

technical field [0001] The invention relates to a paper-based microfluidic chip detection card and a test strip for early rapid screening of chronic obstructive pneumonia aimed at circular genes, belonging to the field of biomedicine. Background technique [0002] Chronic obstructive pulmonary disease (COPD) is a disease characterized by airflow limitation, which is not fully reversible, can be accompanied by airway hyperresponsiveness, and develops progressively. Associated with an abnormal inflammatory response of the lungs to noxious gases or particles. COPD is a disease that seriously endangers the health of the public. It is currently the fourth leading cause of death in the world and will become the third leading cause of death by 2020. COPD has also caused a severe economic burden. The World Bank / World Health Organization (WHO) announced that by 2020, COPD will become the fifth largest disease economic burden in the world. (WHO) evaluates c0PD as the first place in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/54306G01N33/54346G01N33/558
Inventor 陈瑞刘宏孙浩李晓波
Owner SOUTHEAST UNIV
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