70results about How to "Intuitive interpretation of results" patented technology

The invention relates to an EML4-ALK (echinoderm microtubule associated protein like4-anaplastic lymphoma kinase) fusion gene fluorescent quantitative PCR (polymerase chain reaction) assay kit, which is applicable to assay of EML4-ALK fusion gene mutation in lung adenocarcinoma. The kit comprises probes, primers and positive controls, which are specially designed for conserved sequences of 9 fusion variations of the EML4-ALK fusion gene. The kit can be used for quickly and accurately assaying 9 most common EML4-ALK fusion gene variations with high sensitivity, namely 9 fusion variations of 7 variant subtypes, which are subtype 1 (E13; A20), subtype 2 (E20; A20), subtype 3 (E6a / b; A20), subtype 4 (E14; A20), subtype 5 (E2a / b; A20), subtype 6 (E18; A20) and subtype 7 (E14; A20), so that a real-time fluorescent quantitative PCR system for assaying 9 most common EML4-ALK fusion gene variations can be established.

The invention discloses a method for detecting nucleic acid by a colloidal gold chromatography technology and a reagent kit, and belongs to the technical field of medical biology. A universal test paper strip for nucleic acid detection is provided; colloidal gold particles are marked on a universal probe 1, and then, the universal probe is fixed on a glass cellulose membrane; the probe sequence is designed into a universal sequence; the test paper strip is fixed on a PVC (polyvinyl chloride) bottom plate; a sample pad, glass fiber, an NC membrane and water absorption paper are in sequential arrangement from the left side to the right side, wherein a T line (detection line) and a C line (quality control line) are arranged on the NC membrane; antibodies to labels b cover the detection line; antibodies to labels a cover the quality control line; the specificity probe A series and the specificity B series successfully combine a gold mark probe and nucleic acid amplification fragments in series; the specificity detection of nucleic acid amplification fragments is realized. The method has the advantages that the technical requirement on the experiment personnel is low; the required detection time is short; special instrument equipment is not needed; the popularization to basic levels and remote rural area medical institutions is easy.

The invention provides a K-Ras gene mutation typing fluorescence quantitative PCR detection kit which comprises PCR mixed reaction solution, a peptide nucleic acid probe, a mutant type K-Ras-AllgloTM fluorescent probe and a positive control sample. The invention further provides a K-Ras gene mutation typing fluorescence quantitative PCR detection method which comprises the following steps: firstly designing a pair of PCR primers and the peptide nucleic acid probe according to K-Ras genes, respectively designing the AllgloTM fluorescent probe against K-Ras gene mutation sites, and adding the PCR reaction solution, the PCR primers, the peptide nucleic acid probe and the AllgloTM fluorescent probe in a reaction system for carrying out fluorescence quantitative PCR detection. The method adopts the PNA technology and is combined with the technology of the AllgloTM fluorescent probe, thereby being capable of quickly and accurately detecting the K-Ras gene mutation sites in various cancer tissues with high sensitivity, and having short time, simple operation and clear, intuitive and safe judgment and reading.

The invention discloses a preparation method and an application method of a nucleic acid rapid detection kit for Bostaurus components in feeds and belongs to the field of molecular biology and immunology. According to the preparation method and the application method, a high-sensitivity and high-specificity method of a polymerase chain reaction in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunology detection; a unique primer is designed and the primer is labeled; an extracted target DNA is subjected to specific amplification and an amplified product is combined with a labeled antibody immobilized on a test strip in a developing solution to form a stable and visible detection strip and a quality control strip, so as to realize rapid and accurate detection on the bovine-derived components in food and feed.

The invention discloses a preparation method of a rapid nucleic acid detection kit for detecting canis familiaris components in feed and an application method thereof, and belongs to the fields of molecular biology and immunology. According to the invention, a high sensitivity and high specificity method of polymerase chain reaction in nucleic acid detection and an immuno gold staining rapid detection technology in an immunological detection are combined, a specific primer is designed and labeled, the extracted target DNA is subjected to specific amplification, and amplified product is combined with a gold labeled antibody immobilized on a test paper tape, so as to form a stable and visible detection band and quality control band, thereby achieving the rapid and correct detection of the canis familiaris components in the main feed.

The invention relates to a fluorescence quantitative PCR detection kit for beta-thalassemia mutation. The kit comprises a PCR mixing reaction liquid, a positive control and fluorescence probes for detecting beta-thalassemia mutation genotype, wherein the PCR mixing reaction liquid contains PCR primers for amplifying a gene fragment on which a mutation site is positioned, and the mutation site is at least one mutation site selected from deletion mutation of a base corresponding to the site 41 / 42 amino acid of beta-globin gene, C-to-T mutation of a base corresponding to the site 654 amino acid of the second intron of beta-globin gene, A-to-T mutation of a base corresponding to the site 17 amino acid of beta-globin gene, A-to-G mutation of a base corresponding to the site 28 amino acid on the upstream of the promoter of beta-globin gene, A base insertion mutation of a base corresponding to the site 71 / 72 amino acid of beta-globin gene, and G-to-C mutation of a base corresponding to the site 5 amino acid of the first intron of beta-globin gene. With the technical scheme of the present invention, rapid, accurate and high sensitivity detection of mutation conditions of beta-thalassemia gene can be achieved, and especially 6 special site mutations of beta-thalassemia gene can be detected, wherein the 6 special site mutations are common in Chinese.

The invention provides a kit for simultaneously detecting mutations in mitochondria DNA A1555G and C1494T related to maternally inherited drug-induced deafness and a using method thereof. The kit comprises a reagent for extracting sample genomic DNA, a PCR amplification reactive reagent, a primer mixed liquor, a positive control template and a negative control template. The using method of the kit mainly comprises the following steps: using blood, hair with follicle, oral mucosa doctor blade, saliva, and the like, as a sample; adopting a proteinase K digestion pyrolysis method to extract genomic DNA; and then simultaneously detecting mutations in A1555G and C1494T by multiple allele specific PCR. The kit is used for detecting the mutations in mitochondria DNA A1555G and C1494T related to maternally inherited drug-induced deafness and is more rapid, economical and simpler than the single detection for mutation in A1555G or C1494T, and the kit has low requirements for equipment and environment and is conducive to promotion and application.

The invention provides a diagnostic kit for obesity gene mutation. Probe sequences are designed according to the principle of sequence reverse complementation in the direction from 5' to 3' specific to the coding sequence of obesity-related pathogenic genes, and the oligonucleotide in situ synthesis technology is adopted on a connecting joint of each probe sequence to conduct large-scale synthesisof oligonucleotide on a chip, the oligonucleotide on the chip is eluted with ammonia water to form an oligonucleotide mixture, and a biotin-labeled primer at 5' end is adopted to form a DNA probe library of biotin-labeled obesity-related pathogenic genes in a PCR method. A high-throughput screening method and application for obesity-related known sites are achieved, are faster, more economical and simpler than detection of single site-specific screening and whole-genome or whole-exon sequencing, have low equipment and environmental requirements, can be used in the polymorphic sites of obesity-related known nuclear genes, and is suitable for large-scale screening and preventive examination of obesity-related people.

The invention discloses a nucleic acid rapid detection kit for a geese origin component in food and feed and an application method of the kit, belonging to the fields of molecular biology and immunology. According to the invention, a high-sensitivity high-specificity method of PCR in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunological detection, extracted target DNA is subjected to specific amplification through designing a specific primer and labeling the primer and the amplified product is combined with a gold labeled antibody immobilized on the test strip in a developing liquid so as to form stable and visible detection zone and quality control zone, thereby realizing the rapid and accurate detection of geese origin component in food and feed.

The invention discloses a PIK3CA gene mutation fluorescence quantitative PCR genotype detection kit comprising a PCR mixed reaction liquid, a positive control, and a fluorescent probe used for detecting PIK3CA gene mutant genotypes. The PCR mixed reaction liquid comprises PCR primers used for amplifying PIK3CA gene segments where the mutation sites exist. The invention also discloses a PIK3CA gene mutation fluorescence quantitative PCR genotype detection method. According to the method, PIK3CA gene mutant genotypes are subjected to fluorescence quantitative PCR detections by using the kit provided by the invention. With the technical scheme provided by the invention, detections can be carried out upon tissue PIK3CA gene mutations, and especially rapid, accurate, and high-sensitively detections upon PIK3CA gene 542,545 and 1047 nucleotide mutations can be realized.

The invention discloses a preparation method and an application method of a nucleic-acid rapid detection kit for Gallusgallus ingredients in food and feeding stuff, and belongs to the fields of molecular biology and immunology. The detection kit is characterized in that the high-sensitivity and high-specificity method of the polymerase chain reaction in nucleic acid detection is combined with the immune colloidal gold rapid detection technology in immunological detection, unique primers are designed and marked, the extracted target DNA is specifically amplified and the product of the amplification is bonded with gold labeled antibodies immobilized on a test strip in a developing solution, and as a result, stable and visual detection zone and quality control zone are formed, and therefore, rapid and accurate detection on the Gallusgallus ingredients in the food and the feeding stuffing is realized.

The invention discloses a rapid nucleic acid test strip detection kit for a food-borne pathogenic microorganism cronobacter sakazakii and an application method of the kit, and belongs to the fields of molecular biology and immunology. According to the kit and the application method thereof, a high-sensitivity and high-specificity polymerase chain reaction method for nucleic acid detection is combined with an immune colloidal gold rapid-detection technology for immunological detection, unique primers are designed and labeled, extracted target DNA (deoxyribonucleic acid) is subjected to specific amplification, and an amplification product is bound with a gold labeled antibody fixed on a test strip in a developing solution to form a stable and visible detection strip and a stable and visible quality control strip, so that main food-borne pathogenic microorganisms are rapidly and accurately detected.

The present invention discloses a preparation method of a rapid nucleic acid detection kit for a donkey (Equusafricanusasinus) component in food and feed, and an application method thereof, and belongs to the field of molecular biology and immunology. According to the invention, the high sensitivity and high specificity polymerase chain reaction method in nucleic acid detection and the immune colloidal gold rapid detection technology in immunology detection are combined, the unique primers are designed, the primers are labeled, specific amplification is performed on the extracted target DNA, and the amplified product is bound with gold-labeled antibody immobilized on test paper strip in a spreading solution so as to form the stable and visible detection zone and the quality control zone, such that the rapid and accurate detection on the donkey source component in food and feed is achieved.

The invention provides a gene detection method of Leber's hereditary optic neuropathy, (LHON), a gene chip and a kit. The kit comprises a plurality of PCR probes and primers for detecting SNP of optic nerve lesion genes. The chip is provided with detection probes aiming at 13 mitochondrial genes and 46 SNP loci. According to the gene detection method, the kit and the gene chip are utilized to perform detection, the gene detection method comprises the following steps: performing amplification and hybridization on marked samples; scanning hybridization signals; obtaining chip data, and processing the obtained chip data; judging SNP loci, and judging and reading SNP loci information of the visual lesion genes. The gene detection method disclosed by the invention overcomes the detects that by adopting the direct sequencing, the using sample volume is large and the blood sample quantity is large, the amplification time is long, the amplification frequency is high, and false positive or false negative results can be easily caused, and the like, so that the pain of detected persons can be relieved, and not only be the detection accuracy improved, but also the detection time is shortened. According to the invention, the chip and the kit which have the characteristics of being rapid and being high in flux are provided, so that the early diagnosis and treatment of diseases can be facilitated, and nationwide large-scale screening and preventive inspection are convenient to carry out.

The invention provides a kit for detecting deaf related mitochondrial DNA T7505C mutation. The kit is composed of a DNA extraction mixed liquid, a PCR mixed liquid for T7505C fragment amplification, a pair of outer primers designed against T7505C, a pair of inner primers designed against the T7505C, a restrictive endonuclease, a positive contrast, a negative contrast and a kit body. A protease K digestion cracking method is utilized in the invention to rapidly extract genome DNA from a small amount of specimens, so a problem of the traditional extraction of DNA from the blood of a deaf patient is solved, the pains of a detected person is mitigated, and a trans-regional sample transmission problem is also solved. The kit has the advantages of low application cost, simple and rapid detection flow, visual result interpretation, ensuring of the specificity and the stability of the detection result, and realization of the application in the detection of the deaf related mitochondrial tRNAGlnT7505C mutation.

The invention discloses an RET fusion gene ARMS (amplification refractory mutation system) fluorescent quantitative PCR typing detection kit and relates to a primer, a probe and a detection kit for detecting 7 common KIF5B-RET fusion gene variants and 1 RET fusion gene type ARMS-qPCR, wherein the RET fusion gene mutation comprises eight fusion variants including KR1(K15:R12), KR2(K16:R12), KR3(K23:R12), KR4(K24:R8), KR5(K22:R12), KR6(K24:R11), KR7(K15:R11) and newly discovered NR8(N6:R12). In the invention, the detection is fast, efficient and sensitive; the result interpretation is very clear and visual, and the result is reliable and peculiar; and by adopting the primer, probe and kit provided by the invention, 8 RET fusion gene mutations can be effectively detected.

This invention belongs to the moleculal biology field. It involves a kind of fluorescent quantitive PCR test kit of detection gene mutations. The test kit includes fluorescent quantitive PCR premixed liquid, fluorescent quantitive reaction liquid A, luorescent quantitive reaction liquid B (the feature is that it contains primer and anti-primer and fluorescence probes), positive comparison sample A, positive comparison sample B, positive comparison sample C. This invention features that: short detection time, easy operation, low quality requirement of sample DNA, the result reading is clear, direct with high sensitivity.

The present invention discloses a preparation method of a rapid nucleic acid detection kit for a duck (scientific name: Anas platyrhynchos) component in food and feed, and an application method thereof, and belongs to the field of molecular biology and immunology. According to the invention, the high sensitivity and high specificity polymerase chain reaction method in nucleic acid detection and the immune colloidal gold rapid detection technology in immunology detection are combined, the unique primers are designed, the primers are labeled, specific amplification is performed on the extracted target DNA, and the amplified product is bound with gold-labeled antibody immobilized on test paper strip in a spreading solution so as to form the stable and visible detection zone and the quality control zone, such that the rapid and accurate detection on the duck source component in food and feed is achieved.

The invention relates to the field of medicines and particularly relates to a kit for detecting gene mutation of deafness. The kit comprises specific primers, a PCR mixed liquid, an enzyme digestion reaction mixed liquid and the like, wherein corresponding specific primers are specially designed for the deaf gene. Mutation sites from G to A of a WFS1 gene c.2389 in the body of a patient can be quickly detected by using the kit, so that large-scaled screening and preventive inspection of deafness related gene mutation on a national scale, particularly in underdeveloped areas can be better carried out. Moreover, the kit is low in detection cost, simple to operate and high in accuracy.

The invention discloses a rapid nucleic acid test strip detection kit for foodborne pathogenic microorganism-listeria monocytogenes and an application method thereof, belonging to the technical field of bacterial examination. The stable and visible detection zone and quality control zone are formed by combining the high-sensitivity and high-specificity polymerase chain reaction method in nucleic acid detection with the immune colloidal gold rapid detection technology in immunological detection, designing unique primers, labelling the primers, specifically amplifying the extracted target DNA and combining the amplification product with gold labelled antibodies immobilized on the test strip in a developing solution, thus achieving rapid and accurate detection of the main foodborne pathogenic microorganism.

The invention discloses a mutation detection lit for Parkinson disease and leukoaraiosis and a detection method thereof, which belong to the field of molecular diagnosis. The kit is provided with a packaging box, two pairs of primer sequences, a PCR (polymerase chain reaction) amplification reaction reagent, an enzyme digestion reaction reagent, a positive standard substance and a negative standard substance. The kit is applied in detection for the related mutations rs2066842 and rs75932628 of Parkinson disease and leukoaraiosis. The detection method comprises the following steps: using software Primer 5.0 and software Oligo 7.0 for obtaining specific PCR amplification products containing rs2066842 and rs75932628 according to the sequences of gene CARD15 and gene TREM2 of human respectively; digesting the specific PCR amplification products by restriction enzymes BamHI and HhaI; and detecting whether the two sites are mutated and the mutation types according to the quantity and size of digestion segments and in combination of the capillary electrophoresis.

The invention relates to an EML4-ALK (echinoderm microtubule associated protein like4-anaplastic lymphoma kinase) fusion gene fluorescent quantitative PCR (polymerase chain reaction) assay kit, which is applicable to assay of EML4-ALK fusion gene mutation in lung adenocarcinoma. The kit comprises probes, primers and positive controls, which are specially designed for conserved sequences of 9 fusion variations of the EML4-ALK fusion gene. The kit can be used for quickly and accurately assaying 9 most common EML4-ALK fusion gene variations with high sensitivity, namely 9 fusion variations of 7 variant subtypes, which are subtype 1 (E13; A20), subtype 2 (E20; A20), subtype 3 (E6a / b; A20), subtype 4 (E14; A20), subtype 5 (E2a / b; A20), subtype 6 (E18; A20) and subtype 7 (E14; A20), so that a real-time fluorescent quantitative PCR system for assaying 9 most common EML4-ALK fusion gene variations can be established.

The invention discloses preparation and applications of a nucleic acid lateral flow test strip kit for detecting EHEC O157, and belongs to the technical field of bacterial examination. According to the preparation and applications, a high-sensitivity and high-specificity method for polymerase chain reaction in a nucleic acid test is combined with an immune colloidal gold quick testing technology in immunological detection, specific amplification is carried out on a target DNA by virtue of a unique marked primer pair, and an amplification product is combined with a gold labeled antibody fixed on a test paper strip so as to form a stable and visible test band and a stable and visible quality control band, and thus the quick and accurate testing on Escherichia coli O157:H7 can be realized.

The invention discloses a Bim (Bcl-2 interacting mediator of cell death) gene deletion fluorescent quantitative PCR (polymerase chain reaction) detection primer, a probe and a detection reagent kit, and belongs to the field of molecular biological detection. The Bim gene deletion fluorescent quantitative PCR detection primer and the probe comprise forward primers 5'-CAACAAACCCATCAGAACAGACAC-3', reverse primers 5'-ACAGCCTCTATGGAGAACAGTGATT-3 and fluorescent probes 5'-FAM-CAGACACTGGAACAAA-MGB-3'. The detection reagent kit comprises the Bim gene deletion fluorescent quantitative PCR detection primer, the probe, premixed liquid of PCR liquid and positive control samples A. The Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit have the advantages that Bim gene deletion mutation can be detected by the aid of the Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit, and the Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit are simple, convenient and feasible and are high in accuracy and sensitivity and particularly suitable for detecting clinical samples; Bim deletion mutation can be detected, and accordingly the Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit can bring convenience for guiding individual treatment on bodies.

The invention discloses a preparation method and an application method of a nucleic acid rapid detection kit for detection of fox (VulpesFox) components in food and feeds, and belongs to the field of molecular biology and immunology. According to the preparation method and the application method, a high sensitivity and high specificity method of polymerase chain reaction for nucleic acid detection and an immune colloidal gold rapid detection technology of immunological detection can be combined, a unique primer is designed, the primer is marked, extracted target DNA is specifically amplified, an amplified product is combined with a gold labeled antibody fixed on a test paper strip in a developing solution for formation of a stable and visible detection band and a quality control band, so that fast and accurate detection of the fox source components in the food and feeds can be realized.

The invention relates to the field of a biological medicine, and particularly relates to a real-time fluorescent PCR (polymerase chain reaction) detection kit for non-deletion type alpha thalassemia. The kit comprises two PCR reagents, namely a PCR reagent I and CPR reagent II, wherein each PCR reagent comprises a primer, a probe, PCR Buffer, dNTPs, MgCl2, DNA (deoxyribonucleic acid) polymerase, UNG (unguentum) enzyme and glycine betaine. By adopting the kit, QS (quality safety) and CS mutant types of the non-deletion type alpha-thalassemia can be simultaneously detected by a single test. The real-time fluorescent PCR detection kit has the characteristics of short detection time, high sensitivity and strong specificity, and is simple to operate; the result can be judged and read after fluorescent PCR is finished; the problem of carrying out complicated operations of hybridization, sequencing and solubility curve analysis on a PCR product in the prior art is overcome.

The invention discloses a preparation method and application method of quick detection kit for nucleic acid of a felissilvestriscatus component in a feed and belongs to the field of molecular biology and immunology. According to the invention, a high-sensitivity high-specificity method for polymerase chain reaction in nucleic acid detection is combined with a quick detection technique for immune colloidal gold in immunology detection, an ingenious primer is designed and marked, extracted target DNA is subjected to specific amplification, and amplification products are combined with the gold labeled antibody fixed on test strips in amplification liquid to form stable and visible detection strips and quality control strips, thereby realizing the quick and accurate detection for the felissilvestriscatus component in the main feed.

The invention provides a kit for fluorescent quantitative PCR detection of an EML4-ALK fusion gene. The kit comprises a reaction buffer solution, MgCl2, dNTP, a primer mixed solution I, a primer mixedsolution II, a primer mixed solution III, a primer mixed solution IV, a primer mixed solution V, a primer mixed solution VI, a primer mixed solution VII, a primer mixed solution VIII, DNA polymerase,purified water and a positive control, the concentration of each of above primers and probes is 10 [mu]mol, and a volume ratio of a primer to a probe in every mixed solution is 2.5:1. A amplificationreaction system of the kit includes 0.2 [mu]l of the DNA polymerase, 1.2 [mu]l of the primer mixed solution, 2 [mu]l of a sample / positive control / purified water, 12.1 [mu]l of purified water, 2 [mu]lof MgCl2, 2 [mu]l of 10 * buffer and 0.5 [mu]l of the dNTP. The kit has a high detection sensitivity and a good specificity; the specific primers and fluorescent probes are designed for seven variants of the EML4-ALK fusion gene, and a relevant reaction solution is detected in tubes to eliminate the interference among the primers, so the detection specificity and the sensitivity in the inventionare greatly higher than those of a case adopting a mixed reaction solution, and the false positive rate is low.

The invention discloses a preparation method and an application method of a nucleic acid rapid detection kit for detection of rabbit source (Leporidae) components in food and feeds, and belongs to the field of molecular biology and immunology. According to the preparation method and the application method, a high sensitivity and high specificity method of polymerase chain reaction for nucleic acid detection and an immune colloidal gold rapid detection technology of immunological detection can be combined, a unique primer is designed, the primer is marked, extracted target DNA is specifically amplified, and an amplified product is combined with a gold labeled antibody fixed on a test paper strip in a developing solution for formation of a stable and visible detection band and a quality control band, so that fast and accurate detection of the rabbit source components in the food and feeds can be realized.

The invention discloses an alpha-thalassemia related gene detection kit. The invention provides an alpha-thalassemia detection primer group for detecting alpha globin fusion gene, particularly primers with nucleotide sequences as shown in SEQ ID NO: 2-3, and a detection kit constructed based on the alpha-thalassemia detection primer group; and the operation is simple, the cost is low, the specificity is high, the result interpretation is visual, the popularization is easy, and the alpha-thalassemia detection primer group is suitable for being used in various occasions. Moreover, the primer group also has a very good multi-system general advantage, and can be matched with other detection primers for multiple PCR detection, for example, primers with nucleotide sequences as shown in SEQ ID NO: 4-5 or primers with nucleotide sequences as shown in SEQ ID NO: 6-7 can be used for detecting various types of alpha-thalassemia at one time. The technical scheme provided by the invention is of great significance to screening, genetic counseling and prenatal diagnosis of crowds suffering from thalassemia.