The invention provides a kit for fluorescent quantitative PCR detection of an EML4-ALK fusion gene. The kit comprises a reaction buffer solution, MgCl2, dNTP, a primer mixed solution I, a primer mixedsolution II, a primer mixed solution III, a primer mixed solution IV, a primer mixed solution V, a primer mixed solution VI, a primer mixed solution VII, a primer mixed solution VIII, DNA polymerase,purified water and a positive control, the concentration of each of above primers and probes is 10 [mu]mol, and a volume ratio of a primer to a probe in every mixed solution is 2.5:1. A amplificationreaction system of the kit includes 0.2 [mu]l of the DNA polymerase, 1.2 [mu]l of the primer mixed solution, 2 [mu]l of a sample / positive control / purified water, 12.1 [mu]l of purified water, 2 [mu]lof MgCl2, 2 [mu]l of 10 * buffer and 0.5 [mu]l of the dNTP. The kit has a high detection sensitivity and a good specificity; the specific primers and fluorescent probes are designed for seven variants of the EML4-ALK fusion gene, and a relevant reaction solution is detected in tubes to eliminate the interference among the primers, so the detection specificity and the sensitivity in the inventionare greatly higher than those of a case adopting a mixed reaction solution, and the false positive rate is low.