7 results about "Cronobacter" patented technology

The application discloses application of antibacterial peptide BMAP-28 and antibiotics in combined preparation of antibacterial drugs and belongs to the technical field of biological medicine. The antibiotics are selected from one or more of the following: ciprofloxacin, gentamicin and cefixime. The application combines the antibacterial peptide BMAP-28 with antibiotics such as ciprofloxacin and gentamicin, finds that the combination has a significant synergistic antibacterial effect on Enterobacter sakazii, can greatly save the dosage of the drug, reduce the toxic side effect and block the evolution process of drug resistance. Through the destruction of the bacterial cell membrane and the assistance of the antibiotic effect, the combination can effectively overcome the multiple drug resistance, widen the antibacterial spectrum and has a wide clinical application prospect.

The present invention discloses a method for detecting Cronobacter sakazakii and its application. The method comprises: identifying a sequence rich in AT bases in the genome sequence of Cronobacter sakazakii as a target sequence; designing specific primers for the target sequence in an automated and high-throughput manner; designing a specific Tm value calculation formula to calculate the reaction denaturation and annealing temperature, and setting the nucleic acid amplification reaction conditions accordingly; and performing a nucleic acid amplification reaction under the condition of local melting of the template. The present invention targets sequences rich in AT bases that are usually avoided by traditional nucleic acid amplification methods, greatly broadening the candidate target region; through specific screening based on massive genomic data, and accurate calculation of the Tm value for AT-rich regions, local melting of double-stranded DNA is achieved, reducing the possibility of nonspecific amplification in nucleic acid amplification technology. This method effectively expands the scope of application of traditional nucleic acid amplification technology, while significantly improving reaction performance in terms of primer design flux, reaction specificity, etc.

The invention relates to the technical field of probiotics, in particular to lactobacillus rhamnosus FJS004 for inhibiting food-borne pathogenic bacteria, lactein of lactobacillus rhamnosus FJS004 and application of lactobacillus rhamnosus FJS004. The lactobacillus rhamnosus FJS004 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 26, 2022, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.26253. The lactobacillus rhamnosus FJS004 can be used for preparing the lactobacillus rhamnosus FJS004. The lactobacillus rhamnosus FJS004 has the advantages that the lactobacillus rhamnosus FJS004 can be used for preparing the lactobacillus rhamnosus FJS004; the lactobacillus rhamnosus FJS004 and the lactein of the lactobacillus rhamnosus FJS004 provided by the invention have a good bacteriostatic or bactericidal effect on escherichia coli, salmonella, shigella, staphylococcus aureus, listeria monocytogenes, pseudomonas aeruginosa, bacillus cereus and enterobacter sakazakii.

The present invention belongs to the field of molecular biology application technology, and specifically relates to a primer pair, a kit and a method for detecting Cronobacter sakazakii. The nucleotide sequence of the target gene fragment is shown in SEQ ID NO.1; the primer pair is designed according to the target gene fragment, including an upstream primer mngB-F and a downstream primer mngB-R, and their sequences are shown in SEQ ID NO.2 and SEQ ID NO.3, respectively. The primer pair and RPA detection method of the target gene fragment based on the new target provided by the present invention solve the problem that the target gene types of the existing Cronobacter sakazakii nucleic acid detection are few and the specificity is not strong, and has the advantages of strong broad spectrum, good specificity, high sensitivity, high accuracy, simple operation and low cost, and has good application prospects in the field of food detection.

The invention relates to a fluorescence detection method for respectively and rapidly detecting Cronobacter mojinensis and Cronobacter kangdi by adopting a body temperature amplification (ERA) technology. The invention also relates to an oligonucleotide primer probe composition for the method. The invention also relates to a detection kit containing the composition. When the composition provided by the invention is used for ERA fluorescence method detection, cronobacter mojinensis and cronobacter kangdi can be simply, quickly, specifically and sensitively detected.

The invention relates to a primer probe combination, a digital PCR (Polymerase Chain Reaction) detection kit and a method for absolute quantitative detection of cronobacter in food. The primer probe combination comprises a recN-F upstream primer, a recN-R downstream primer and a recN-P probe, the nucleotide sequence of the recN-F upstream primer is as shown in SEQ ID NO.1, the nucleotide sequence of the recN-R downstream primer is as shown in SEQ ID NO.2, and the nucleotide sequence of the recN-P probe is as shown in SEQ ID NO.3. The invention further discloses a kit for detecting the protein content of the protein. Components participating in amplification are divided into millions of PCR reactions which obey Poisson distribution, then each reaction is subjected to PCR amplification, then the positive and negative properties of reaction results are judged by reading fluorescence signals, and finally the copy number and concentration of target molecules are calculated according to the judgment result according to the Poisson distribution principle, so that absolute quantification is achieved.

The invention relates to two rapid detection methods, namely a fluorescence method and a test strip method, for rapidly detecting cronobacter malonate by adopting a body temperature amplification (ERA) technology. The invention also relates to an oligonucleotide primer probe composition for the method. The invention also relates to a detection kit containing the composition. When the composition is used for detection by an ERA fluorescence method and a test strip method, the cronobacter malonate can be simply, rapidly, specifically, sensitively and visually detected.