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32 results about "Cronobacter" patented technology
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Cronobacter is a genus of Gram-negative, facultatively anaerobic, oxidase-negative, catalase-positive, rod-shaped bacteria of the family Enterobacteriaceae. They are generally motile, reduce nitrate, use citrate, hydrolyze esculin and arginine, and are positive for L-ornithine decarboxylation. Acid is produced from D-glucose, D-sucrose, D-raffinose, D-melibiose, D-cellobiose, D-mannitol, D-mannose, L-rhamnose, L-arabinose, D-trehalose, galacturonate and D-maltose. Cronobacter spp. are also generally positive for acetoin production (Voges–Proskauer test) and negative for the methyl red test, indicating 2,3-butanediol rather than mixed acid fermentation. The type species of the genus Cronobacter is Cronobacter sakazakii comb. nov.
Nucleotide specific to cronobacter O antigen and application of nucleotide
ActiveCN103898227APracticalThe preparation method is simple and easyMicrobiological testing/measurementMicroorganism based processesAntigenNucleotide
The invention discloses a nucleotide specific to a cronobacter O antigen and an application of the nucleotide. The nucleotide is at least one of nucleotides as shown in SEQ ID NO: 1-12 or at least one of complementary nucleotides. The specific nucleotide can be used for preparing a PCR (Polymerase Chain Reaction) kit and / or a gene chip for quickly detecting the cronobacters. The kit is simple and convenient, high in accuracy, good in sensitivity, excellent in operability, good in repeatability and low in detection cost if being applied to detecting clinical cronobacters or cronobacters in food.
Owner:NANKAI UNIV
Tracing method of cronobacter spp. in infant formula milk powder
The invention provides a tracing method of cronobacter spp. in infant formula milk powder. The method comprises the steps that by means of a multiple-locus sequence typing technology, molecular typing research is conducted on the cronobacter spp. of PIF raw materials, semi-finished products, finished products and processing environments thereof, population characteristics of the cronobacter spp. are revealed, a phylogenetic relationship of the cronobacter spp. is confirmed, phylogenetic analysis is conducted on the cronobacter spp. through bioinformatics, and therefore genetic characteristics of the cronobacter spp. in the above-mentioned environments are revealed systematically; on the basis of cronobacter spp. MLST typing, correlation analysis is conducted by taking ST of pathogenic bacteria and environmental sources as variables, key links which are most prone to pollute the cronobacter spp. in the PIF processing process and distribution rules are determined, and therefore the source of the cronobacter spp. in the infant formula milk powder is traced. The purpose of fundamentally monitoring the PIF production process and ensuring the PIF quality are achieved.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Cronobacter broth culture medium and detection method
InactiveCN102286606ARealize authenticationShorten the timeMicrobiological testing/measurementFluorescence/phosphorescenceBiotechnologyGlucoside
The invention relates to a detection medium and a detection method for Cronobacter in food. The detection method of the present invention utilizes the activity of alpha-glucosidase produced by Cronobacter to detect Cronobacter in liquid culture medium, and can identify Cronobacter while carrying out selective enrichment. The Cronobacter broth medium of the present invention may comprise 18-20 parts by weight of mixed peptone; 3-5 parts by weight of lactose; 2-3 parts by weight of K2HPO4; 2-3 parts by weight of KH2PO4; 20-34 parts by weight of sodium chloride 0.08-0.15 parts by weight of sodium lauryl sulfate; 0.009-0.011 parts by weight of vancomycin; 0.07-0.10 parts by weight of 4-methylumbelliferone-α-D-glucopyranoside; pH6.8 ± 0.2, the best Culture temperature, 36°C. The invention also discloses a reagent kit for the above detection method. The invention has the advantages of shortening the detection time and avoiding subsequent tedious identification operation steps, thereby improving the detection efficiency of Cronobacter.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE +1
Rapid detection kit for Cronobacter sakazakii
InactiveCN108285925AReduced operating requirementsSimple and fast operationMicrobiological testing/measurementMicroorganism based processesLysisPositive control
The invention discloses a rapid detection kit for Cronobacter sakazakii. The kit comprises a dry powder detection reagent, reconstitution fluid, positive control dry powder, negative control dry powder, confining liquid, a developing solution and a lysis solution. Six primers in the kit correspond to eight sectors of a specific target gene sequence of the Cronobacter sakazakii; by adding the developing solution, the negative and positive developing difference is obvious, and the rapid detection kit has the advantages of high specificity, high sensitivity, easiness in operation, simple result judgment and the like and is suitable for rapid detection of the Cronobacter sakazakii in the field of food safety and sanitation. Besides, the rapid detection kit is a dry powder detection reagent, isconvenient to transport at normal temperature and is simple and convenient to use; no special instrument is needed, so that the cost is reduced and application range of a product is widened; the rapid detection kit is more suitable for rapid detection of the Cronobacter sakazakii in field and basic units of remote regions and has broad application prospect.
Owner:GUANGDONG HUANKAI MICROBIAL SCI & TECH +1
Detecting method for Cronobacter sakazakii as well as kit and primers used in detecting method
ActiveCN103966353AShort detection timeReduce testing costsMicrobiological testing/measurementDNA/RNA fragmentationFood safetyMicrobiology
The invention discloses a detecting method for Cronobacter sakazakii as well as a kit and primers used in the detecting method. The method comprises the following steps of (1) extracting a genome DNA (Deoxyribonucleic Acid) of a sample to be detected; (2) enabling primers with sequences as shown in SEQ ID NO.1 and SEQ ID NO.2 to be subjected to PCR (Polymerase Chain Reaction) by taking the genome DNA obtained in the step (1) as a template; and (3) detecting the existence of a single amplification product 498bp in the reaction product obtained in the step (2). The primers include the primers as shown in SEQ ID NO.1 and SEQ ID NO.2. The kit comprises the primers. The method disclosed by the invention is short in detection time, low in cost, single in specificity, reliable in detecting result and simple in result judgment; and in addition, the invention provides the simple, rapid and sensitive detecting method for Cronobacter sakazakii in the technical field of food safety detection, and the detecting method has important significance for food safety in China.
Owner:BRIGHT DAIRY & FOOD CO LTD
Nucleotide specific to Cronobacter O antigen and application thereof
ActiveCN104862393APracticalThe preparation method is simple and easyMicrobiological testing/measurementMicroorganism based processesAntigenNucleotide
The invention discloses a nucleotide specific to a cronobacter O antigen and an application of the nucleotide. The nucleotide is at least one of nucleotides as shown in SEQ ID NO: 1-12 or at least one of complementary nucleotides. The specific nucleotide can be used for preparing a PCR (Polymerase Chain Reaction) kit and / or a gene chip for quickly detecting the cronobacters. The kit is simple and convenient, high in accuracy, good in sensitivity, excellent in operability, good in repeatability and low in detection cost if being applied to detecting clinical cronobacters or cronobacters in food.
Owner:NANKAI UNIV
Primer probe for RNA isothermal amplification to detect cronobacter, kit and detection method
ActiveCN108424973AEfficient detectionStrong specificityMicrobiological testing/measurementMicroorganism based processesCronobacterBiology
The invention discloses a primer probe for RNA isothermal amplification to detect cronobacter, a kit and a detection method. The provided primer probe for detecting specificity, the detection kit containing the primer probe, and the detection method using the detection kit to carry out RNA isothermal amplification have the characteristics of high sensitivity, strong specificity, low pollution (amplification products RNA are easily degraded in nature), and rapidness; the requirements on instruments are low; the operation is simple; the primer probe and the kit are suitable for self detection offood enterprises and basic detection mechanisms and onsite detection, in particular, milk powder rapid detection, and the safety of milk powder is guaranteed.
Owner:健垣科技(张家口)有限公司
Highly specific gene segment of Cronobacter spp. and application thereof
InactiveCN103468718AHigh detection specificityStrong specificityMicrobiological testing/measurementFermentationSpecific detectionMicrosphere
The invention relates to a method for specific detection of Cronobacter spp. with silica magnetic microballoon and nested PCR technology based on a Cronobacter spp. 16SrDNA gene specific sequence, which is shown in the SEQID NO.1. Under specific condition, the property that silica magnetic microballoons can absorb nucleic acids is used for realizing rapid adsorption, enrichment and separation of pathogen nucleic acids in milk powder samples; two pairs of highly specific primers are designed based on Cronobacter spp. 16SrDNA gene, Cronobacter spp. specific gene is amplified by nested PCR technology, thereby realizing accurate, rapid and high sensitivity detection of Cronobacter spp. existed in the samples.
Owner:WUXI ZODOLABS BIOTECH
Cronobacter and application thereof
The invention discloses a cronobacter with the preservation number being CGMCC No. 11032. The cronobacter can be used for preparing XFP-gy bactericide for enhancing degradation of residual chlopyrifos in crops; compared with the prior art, the cronobacter can effectively degrade chlopyrifos residues in an environment, be fixed to crop roots for a long time, enhance the activity of the crops in degrading residual chlopyrifos and efficiently degrade pesticide residues at the premise that quality of the crops is not affected and is green and free of pollution; the cronobacter is extremely high in chlopyrifos degradation capability, simple in culture method, high in growth speed, not prone to variation and capable of being directly applied to degradation of chlopyrifos in soil and pesticide production waste water, crops such as paddy rice can be inoculated to serve as endophyte to be used for chlopyrifos residue control, and the cronobacter is suitable for being widely used and popularized.
Owner:JIANGSU ACAD OF AGRI SCI
Application of reagent for detecting flora in preparation of reagent or kit for prognosis prediction markers of esophageal squamous cell carcinoma patients
ActiveCN111269956AImproved prognosisRealize precise treatmentMicrobiological testing/measurementMicroorganism based processesFamily HalomonadaceaeOncology
The invention provides application of a reagent for detecting flora in preparation of a reagent or a kit for prognosis prediction markers of esophageal squamous cell carcinoma patients, and belongs tothe technical field of prognosis detection of esophageal squamous cell carcinoma. In the present invention, the flora comprises one or multiple selected from Cronobacter in cancer tissues of patientswith esophageal squamous cell carcinoma, Family Halomonadaceae in para-carcinoma tissue of esophageal squamous cell carcinoma patients, and Carnobacterium in para-carcinoma tissue of esophageal squamous cell carcinoma patients. According to the invention, microorganism-related evidences are provided for prognosis prediction of esophageal squamous cell carcinoma, and a reference basis is providedfor prognosis prediction of esophageal squamous cell carcinoma patients.
Owner:FUJIAN MEDICAL UNIV
Rapid detection method of enterobacter sakazakii in dairy products
PendingCN111394482ARealize joint detectionAvoid False Positive InterferenceMicrobiological testing/measurementAgainst vector-borne diseasesBiotechnologyMicrobiology
The invention discloses a rapid detection method of enterobacter sakazakii in dairy products. The rapid detection method comprises the following steps: firstly, extracting dairy product samples and extracting RNA (ribonucleic acid) of more dairy product samples; adding 1-5 micrograms of the RNA in step 1 into a 0.5ml microcentrifuge tube and supplementing DEPCH2O to enable the total volume to reach 11 mu l; adding 10 microns Oligo (dt)12-18 microliters into the tube; then uniformly shaking and centrifuging; after heating at 70 DEG C for 10 min, immediately inserting the microcentrifuge tube instep 2 into an ice bath for 1 min. According to the rapid detection method of the enterobacter sakazakii in the dairy products, disclosed by the invention, mRNA (messenger ribonucleic acid) is used as a target gene for detection and can be detected through methods including PCR (polymerase chain reaction) and the like; the mRNA can be rapidly degraded after bacteria are dead, so that the proper mRNA is selected as a target gene and only the bacteria with activity can be detected, and false positive interference caused by dead bacteria is avoided; and meanwhile, a PCR primer is improved and designed and a real-time fluorescent RT-PCR technology is applied, so that combined detection of various bacteria can be realized and the time and the cost are saved.
Owner:西安市食品药品检验所 +1
Sensitization test strip for rapidly detecting cronobacter sakazakii and application of sensitization test strip
The invention discloses a sensitization test strip for rapidly detecting cronobacter sakazakii and application of the sensitization test strip, and belongs to the technical field of detection of cronobacter sakazakii. The sensitization test strip comprises a sample pad, a gold standard pad, an NC film, an absorbent pad and a bottom plate, wherein the NC film is provided with a detection line and acontrol line, the control line is coated with a goat anti-mouse secondary antibody, and a detection line solution coating the detection line is obtained by incubating colloidal gold and an anti-cronobacter sakazakii capture antibody. The sensitization test strip has the characteristic of being capable of improving the protein binding quantity on the NC film, thereby enhancing the capture rate; the colloidal gold added at the detection line can improve aggregation coloration, thereby improving the detection effect; and the sensitivity is improved by 100 times compared with a traditional test strip, and the sensitization test strip has high specificity, short detection time, easy manufacture and a low cost, and is easy to carry and suitable for on-site detection of cronobacter sakazakii.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Cleaning agent for inhibiting Cronobacter biofilm and its preparation method and application
ActiveCN109456853BChange permeabilityInterfere with normal metabolismDetergent mixture composition preparationAmpholytes/electroneutral surface-active compoundsBiotechnologyActive agent
The invention relates to a cleaning agent for inhibiting Cronobacter biofilm, a preparation method and application thereof. The cleaning agent includes amino oligosaccharides or derivatives thereof or fucoidan oligosaccharides, anionic surfactants, zwitterionic surfactants, nonionic surfactants, glycerin and water. The cleaning agent of the invention has a good inhibitory effect on Cronobacter biofilm, especially can effectively inhibit the adhesion of bacteria in the early stage of film formation. Compared with common commercial cleaning agents, the new cleaning agents containing amino oligosaccharides or their derivatives or alginate oligosaccharides, anionic surfactants, zwitterionic surfactants, and nonionic surfactants have significantly improved inhibitory effects on biofilms.
Owner:EAST CHINA UNIV OF SCI & TECH
Application of epsilon-polylysine or hydrochloride thereof in preparation of medicine for inhibiting cronobacter spp or intervening cronobacter spp biofilm
PendingCN113768949AImprove the bactericidal effectImprove securityAntibacterial agentsOrganic active ingredientsBiotechnologyStaining
The invention discloses application of epsilon-polylysine or hydrochloride thereof in preparation of a medicine for inhibiting cronobacter spp or intervening a cronobacter spp biofilm. Experimental results show that epsilon-polylysine has the effects of inhibiting formation of the cronobacter spp and the biofilm thereof and removing the mature biofilm of the cronobacter spp. According to the invention, the bacteriostatic activity of the epsilon-polylysine to the cronobacter spp is determined by adopting a standard trace broth dilution method, the MIC value is 0.125-0.25 mg / mL, and the MBC value is 0.125-0.50 mg / mL; and by a crystal violet staining method and scanning electron microscope observation, the invention finds that the epsilon-polylysine with sub-inhibitory concentration has a remarkable inhibition effect on formation of the cronobacter spp biofilm, and the epsilon-polylysine with high concentration can destroy the mature biofilm. The invention provides experimental basis for clinical application of the epsilon-polylysine in the aspect of preparation of drugs for inhibiting the cronobacter spp or intervening the biofilm of the cronobacter spp, and the epsilon-polylysine has good application prospects and huge potential value.
Owner:XUZHOU MEDICAL UNIV
Two attenuated lipid A producing Cronobacter mutant strains and application thereof
InactiveCN108265020AImproved outer mold permeabilityReduce the level of infestationAntibacterial agentsBacterial antigen ingredientsGenetic engineeringMutant strain
The invention discloses two attenuated lipid A producing Cronobacter mutant strains and application thereof, and belongs to the technical field of genetic engineering. Through knocking out lpxM gene fragments of C.sakazakii BAA894 and ATCC 51329 bateria, the outer mold seepage performance of the strains is improved; the resistance on cationic anti-microbial peptides is reduced; the strains can bemore easily killed by the anti-microbial peptides; the copying survival capability of the C.sakazakii BAA894 and ATCC 51329 in mouse RAW264.7 cells and the stimulation effect on the HEK4 cells can bereduced; the infection level of the strains in macrophages is reduced; the cell toxicity is weakened; certain help is realized in the later development of vaccine adjuvants.
Owner:JIANGNAN UNIV
Primer combination and kit for simultaneously detecting cronobacter and salmonella and application of primer combination and kit
PendingCN113699258ARealize visual detectionEasy to prepareMicrobiological testing/measurementMicroorganism based processesBiotechnologySalmonella orion
The invention discloses a primer combination and a kit for simultaneously detecting cronobacter and salmonella and application of the primer combination and the kit and belongs to the technical field of food safety. In order to solve the problems existing in detection on the salmonella and the cronobacter by a traditional microbiological method, the invention provides a dual test strip based on a LAMP reaction and a primer combination as shown in SEQ ID NO: 1-8, and the dual test strip comprises the following steps: extracting DNAs (deoxyribonucleic acids) from the salmonella and the cronobacter, and amplifying a large number of target products through the LAMP reaction; and directly interpreting a result by naked eyes through the dual test strip constructed on the basis of preparation of stable colloidal gold, a gold-labeled antibody, a redissolution solution and a diluent. The dual test strip obtained by the invention has the advantages of simplicity in manufacturing, low cost, sensitivity in detection and the like, and can be used for simultaneously detecting salmonella and cronobacter pollution in foods and food processing processes, especially pathogenic bacterium pollution in infant formula milk powder.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Cronobacter spp. standard strain containing specific molecular target and detection and application of cronobacter spp. standard strain
ActiveCN112899378AGenetic stabilitySpecial molecular markerBacteriaMicrobiological testing/measurementBiotechnologyHigh survival rate
The invention relates to the technical field of bioengineering, in particular to four cronobacter spp. standard strains which have proprietary intellectual property rights in China and conform to the domestic propagation law, and the preservation numbers of the four cronobacter spp. standard strains are respectively GDMCC 60861, GDMCC 60862, GDMCC 60863 and GDMCC 60864. The four standard strains have typical physiological and biochemical characteristics, are clear in information such as sample sources, genetic backgrounds, drug resistance and the like, and can be used as standard reference strains in different fields such as foods, medicines and clinical examination. The invention also relates to a group of specific target genes for detecting and identifying the four standard strains and corresponding PCR products. Finally, the invention also provides a cronobacter spp. freeze-drying protective agent with high survival rate and strong specificity, and the cronobacter spp. freeze-drying protective agent can be used for long-term storage of the standard strains.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
Multiple lamp detection primers, detection kits and detection methods for six foodborne pathogenic bacteria in fruits and vegetables
InactiveCN107022644BStrong specificityHigh amplification efficiencyMicrobiological testing/measurementMicroorganism based processesBiotechnologyEscherichia coli
The invention discloses a multiplex LAMP detection primer, kit and method for six food-borne pathogenic bacteria in fruits and vegetables and belongs to the technical field of bacterial gene detection. A rapid detection primer set for the six pathogenic bacteria including listeria monocytogenes, enterobacter sakazakii, shigella spp, staphylococcus aureus, salmonella spp and escherichia coli O157:H7 is designed, and multiplex LAMP reaction is performed on the genome DNA of the bacteria extracted from a sample to be detected in the same reaction system by use of the detection kit including the primer set to determine whether the sample contains the six food-borne pathogenic bacteria or not. The multiplex LAMP detection primer is high in specificity and sensitivity and can accurately detect the genome DNA of the six food-borne pathogenic bacteria in the same reaction system, can realize simple and convenient, quick and accurate detection, is suitable for on-site rapid detection and has significance on improving the pathogenic bacterium analysis and detection technology and the fruit and vegetable edible quality security.
Owner:INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
Primer probe combination, kit and method for detecting cronobacter based on RAA technology
PendingCN112795674ANo cross reactionStrong specificityMicrobiological testing/measurementMicroorganism based processesMicroorganismBiochemistry
The invention belongs to the technical field of microbiological detection, and particularly discloses a primer probe combination, a kit and a method for detecting cronobacter based on an RAA technology. The primer probe combination comprises a primer pair and a probe. The primer pair comprises an upstream primer and a downstream primer, the sequence of the upstream primer is as shown in SEQ ID NO.1, the sequence of the downstream primer is as shown in SEQ ID NO.2, and the sequence of the probe is as shown in SEQ ID NO.5. When the kit and the method are used for detecting cronobacter, the specificity is good, the sensitivity is high, the accuracy is high, the operation is simple, the cost is low, laboratory detection and on-site rapid detection can be carried out, and the kit and the method have a good application prospect.
Owner:PLANTS & ANIMALS & FOOD TESTING QUARANTINE TECH CENT SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Real-time fluorescence nucleic acid isothermal amplification detection kit for Cronobacter spp
InactiveCN108265102AReduce pollutionEfficient captureMicrobiological testing/measurementAgainst vector-borne diseasesT7 RNA polymeraseReverse transcriptase
The invention discloses a real-time fluorescence nucleic acid isothermal amplification detection kit for Cronobacter spp. The kit comprises a capture probe, a detection primer T7, an nT 7 primers, a detection probe, M-MLV reverse transcriptase, T7 RNA polymerase and other reagents. The kit can detect the RNA of Cronobacter spp in food and has the advantages of being high in specificity and sensitivity (which can reach 1,000 CFU / ml), low in contamination (wherein amplified product RNA is easily degraded under the natural environment) and capable of achieving rapid detection (which is completedwithin 60 min conventionally), the kit can play an important role in food security, and the application prospect is wide.
Owner:SHANGHAI RENDU BIOTECH
Purified ginkgo leaf flavone and antibacterial application thereof
PendingCN114732838AGood antibacterial effectIncrease bodyAntibacterial agentsGinkgophyta medical ingredientsBiotechnologyGinkgo biloba
The invention discloses a ginkgo leaf flavone purified product and antibacterial application thereof. A purification method comprises a two-step purification method of NKA-9 macroporous resin and polyamide resin. The antibacterial application comprises the application of inhibiting staphylococcus aureus, escherichia coli and cronobacter malonate. The ginkgo leaf flavone purified product disclosed by the invention has a better antibacterial effect on staphylococcus aureus, escherichia coli and cronobacter malonate than that of a crude extract.
Owner:HEFEI UNIV OF TECH
Cronobacter standard strain containing specific molecular target and its detection and application
ActiveCN112899378BEasy to shapeBeautiful appearanceBacteriaMicrobiological testing/measurementBiotechnologyHigh survival rate
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
Cleaning agent with cronobacter spp. biofilm inhibiting effect and preparation method and application of cleaning agent
ActiveCN109456853AHelp preventEasy to makeDetergent mixture composition preparationAmpholytes/electroneutral surface-active compoundsBiofilmBacterial Adhesions
The invention relates to a cleaning agent with a cronobacter spp. biofilm inhibiting effect and a preparation method and application of the cleaning agent. The cleaning agent is prepared from amino oligosaccharides or derivatives thereof or alginate oligosaccharides, an anionic surfactant, a zwitterionic surfactant, a nonionic surfactant, glycerin and water. The cleaning agent has a great cronobacter spp. biofilm inhibiting effect and is especially capable of effectively inhibiting bacterial adhesion in an early stage of film formation. Compared with a common cleaning agent on the market, thecleaning agent containing the amino oligosaccharides or derivatives thereof or alginate oligosaccharides, the anionic surfactant, the zwitterionic surfactant and the nonionic surfactant has the advantage that the biofilm inhibiting effect is remarkably improved.
Owner:EAST CHINA UNIV OF SCI & TECH
Primer group for amplifying cronobacter kronoi MLST (multiple-locus typing) tracing housekeeping genes, next-generation sequencing library building method and application
ActiveCN112094931ASpeed up typingReduce workloadMicrobiological testing/measurementLibrary creationHousekeeping geneMicrobiology
The invention provides a primer group for amplifying cronobacter kronoi MLST (multiple-locus typing) tracing housekeeping genes, a next-generation sequencing library building method and application, and relates to the technical field of next-generation sequencing. The amplification primer group is simultaneously built for seven housekeeping genes specified in Cronobacter Kronoi in the Part 5 of MLST Method for Molecular Typing of Pathogenic Bacteria in Export Food of the industry standard SNT 4525.5-2016. The next-generation sequencing library building method for cronobacter kronoi MLST tracing is built on the basis of the primer group; meanwhile, the amplification of seven genes can be realized through one-time PCR (polymerase chain reaction); the method is directly used for library building; the operation is simple. The sequencing work can be more conveniently performed; the MLST typing speed is accelerated; the workload is reduced.
Owner:上海国际旅行卫生保健中心
Highly specific gene fragment of Enterobacter sakazakii and its application
ActiveCN102816761BHigh detection specificityStrong specificityMicrobiological testing/measurementAgainst vector-borne diseasesSpecific detectionMagnetic bead
The invention relates to a Cronobacter spp. 16S rDNA gene based sequence, and also provides a method for specific detection of Cronobacter spp. by combining magnetic beads and a nested PCR (polymerase chain reaction) technology. The advantage of the invention lies in that: a specific probe and primers of the Cronobacter spp. 16S rDNA gene sequence are utilized, and magnetic beads are combined to conduct rapid and specific capture and enrichment of a Cronobacter spp. target gene in a sample, finally amplification detection is performed through the nested PCR technology, and the detection sensitivity is improved.
Owner:WUXI ZODOLABS BIOTECH
Cronobacter bacteriophage and application thereof
PendingCN114540313AImprove stabilityIncrease temperatureBiocideMicroorganism based processesMicrobiologyBacteriophage
The invention provides a Cronobacter bacteriophage, the Cronobacter bacteriophage is Cronobacter bacteriophage vBCtuPB1, the preservation number of the Cronobacter bacteriophage vBCtuPB1 is GDMCC No: 61185-B1, the Cronobacter bacteriophage is a short-tail bacteriophage, the short-tail bacteriophage is of a polyhedral head and short-tail structure, the head is about 51 mm, and the tail is about 11 mm. The bacteriophage can be used for specifically splitting and splitting the cronobacter malonate and the cronobacter suridatensis. The bacteriophage vBCtuPB1 has good stability in the pH range of 4.0-11 and at the temperature of 25-60 DEG C, does not carry any virulence or antibiotic resistance gene, and can be safely applied to prevention and control of food cronobacter.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
An immunomagnetic bead chromatography test strip and rapid detection method for detecting Cronobacter
ActiveCN108362876BEasy to manufactureAvoid interferenceMicrobiological testing/measurementBiological material analysisPhosphoric acidBovine serum albumin
Owner:HEFEI UNIV OF TECH
Paper-based detection device for detecting cronobacter and application of paper-based detection device
PendingCN113061644AEasy to prepareLow costMicrobiological testing/measurementBiotechnologyMicroorganism
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Cronobacter thuringiensis type 5 lipopolysaccharide O-antigen oligosaccharide fragment and preparation method and application thereof
The invention relates to a cronobacter thelingiensis type 5 lipopolysaccharide O-antigen oligosaccharide fragment and a preparation method and application thereof. The prepared oligosaccharide fragment is a cronobacter thelingiensis type 5 lipopolysaccharide O-antigen tetrasaccharide repeating unit and is prepared by taking various protected monosaccharide modules as raw materials and carrying out glycosylation reaction through a sequential glycosylation strategy, the C-1 site of a pyranosyl group at the reducing end of the oligosaccharide fragment is modified by an amino alkane chain, and can be conjugated with proteins, lipids, polypeptides and the like to prepare oligosaccharide conjugates, and then related immunological research is carried out. The preparation method disclosed by the invention is reasonable in design, strong in operability, simple in separation, high in product yield and good in purity. Immunological research is carried out by synthesizing polysaccharide repeated fragments with different reducing ends, and the method has important significance on research and development of corresponding cronobacter carbohydrate vaccines.
Owner:SHANDONG UNIV
Method for detecting cronobacter in infant formula milk powder
ActiveCN113416765AEfficient enrichmentPromote growthMicrobiological testing/measurementBiological material analysisBiotechnologyAnimal science
The invention relates to a method for detecting cronobacter in infant formula milk powder, and belongs to the technical field of detection of biological products. The innovation lies in improvement of enrichment and separation of cronobacter in infant formula milk powder. A liquid culture medium (dry damage repair factors comprise 0.4-0.8 g / L of mannitol and 2.5-5.0 g / L of trehalose, and selective enrichment factors comprise 80-150 g / L of sucrose, 4-8 g / L of vancomycin and 0.3-0.6 g / L of sodium deoxycholate) is adopted for efficient enrichment, and a solid culture medium (selective components comprise 0.5-1.0 g / L of sodium deoxycholate and 80-100 mg / L of 5-bromo-4-chloro-3-indole-D-alpha-glucoside) is adopted for selective separation. The method has the advantages of high enrichment efficiency, strong selectivity, convenience in operation, short time and the like, and overcomes the defects of long time, low enrichment effect, complicated operation and the like of the existing detection method. The method is suitable for detecting cronobacter in formula milk powder, shortens the detection time, improves the detection accuracy and efficiency, reduces the detection cost, and has important significance in guaranteeing the safety of infant formula milk powder.
Owner:HEFEI UNIV OF TECH
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