Purified ginkgo leaf flavone and antibacterial application thereof
A technology of leaf flavonoids and ginkgo, applied in the field of biomedicine, can solve the problems of low flavonoid extract content and insignificant antibacterial effect, and achieve good antibacterial effect
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[0026] Preparation of three test bacterial solutions: Inoculate Staphylococcus aureus and Escherichia coli preserved in glycerol into LB broth medium, and cultivate at 37°C until the medium is turbid, that is, the activation of the bacteria is completed. The activated bacterial solution was then inoculated into LB broth medium at 1% inoculum, and incubated at 37˚C at 150 rpm for about 7 hours to produce a bacterial concentration of about 106-107CFU / mL.
[0027] Serial double dilution method:
[0028] Pipette 100 µL of the test bacterial solution from row B to row H of the 96-well plate. Pipette 180µL of bacterial solution in row A, and add 20µL of sample or control solution to each well in columns 1-12 of this row. Add two wells for each sample or control as a parallel group. Then use a pipette to mix 100µL from row A to the next row, and so on in turn suck 100µL of mixed solution until the last 100µL of mixed solution is discarded in the last row, to ensure that the solution...
Embodiment 1
[0030] Embodiment 1: a kind of Ginkgo biloba flavonoids purified product and its antibacterial use
[0031] The NKA-9 macroporous resin (Zhengzhou Hecheng New Material Technology Co., Ltd.) was soaked in 95% ethanol solution for pretreatment. After 24h, rinse with distilled water until the effluent is clear and has no alcohol smell. Wet packing was used, and the column was equilibrated for 12 h before loading. The sample solution was prepared according to the following method: the dry powder of ginkgo biloba flavonoid extract was dissolved in 60% ethanol solution to prepare a sample solution of 0.8 mg / mL.
[0032] Load the sample solution on the macroporous resin in the chromatography column at a flow rate of 0.6BV / h; then use 70% ethanol solution as an eluent and elute at a flow rate of 1.0BV / h; collect and wash De-liquid, concentrated and evaporated under vacuum at 45°C, and the flavonoid content in the concentrated solution was measured and adjusted to 4 mg / mL to obtain t...
Embodiment 2
[0035] Embodiment 2: a kind of Ginkgo biloba flavonoids purified product and its bacteriostatic use
[0036]The NKA-9 macroporous resin (Zhengzhou Hecheng New Material Technology Co., Ltd.) was soaked in 95% ethanol solution for pretreatment. After 24h, rinse with distilled water until the effluent is clear and has no alcohol smell. Wet packing was used, and the column was equilibrated for 12 h before loading. The sample solution was prepared according to the following method: the dry powder of ginkgo biloba flavonoid extract was dissolved in 60% ethanol solution to prepare a sample solution of 0.8 mg / mL.
[0037] Load the sample solution on the macroporous resin in the chromatography column at a flow rate of 0.6BV / h; then use 70% ethanol solution as an eluent and elute at a flow rate of 1.0BV / h; collect and wash De-liquid, concentrated and evaporated under vacuum at 45°C, and the flavonoid content in the concentrated solution was measured and adjusted to 4 mg / mL to obtain t...
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