56 results about "Methylumbelliferone" patented technology

A method for the synthesis of a compound of formula (1) or a salt thereof, wherein A is a carbohydrate linker which is a lactosyl moiety or which consists of a lactosyl moiety and at least one monosaccharide unit selected from the group consisting of: glucose, galactose, N-acetylglucosamine, fucose and N-acetyl neuraminic acid; and wherein R1 is one of the following anomeric protecting groups: a) -OR2, wherein R2 is a protecting group removable by catalytic hydrogenolysis; b) -SR3, wherein R3 is an optionally substituted alkyl, an optionally substituted aryl or an optionally substituted benzyl; c) -NH- C(R")=C(R')2, wherein each R' independently is one of the following electron withdrawing groups: -CN, -COOH, -COO-alkyl, -CO-alkyl, -CONH2, -CONH- alkyl or -CON(alkyl)2, or wherein the two R'-groups are linked together and form -CO-(CH2)2-4-CO- and thus form, together with the carbon atom to which they are attached, a 5-7 membered cycloalkan-1,3-dione, in which dione any of the methylene groups is optionally substituted with 1 or 2 alkyl groups, and R" is H or alkyl, in which a fucosyl donor of formula (2) wherein X is selected from the group consisting of: a guanosine diphosphatyl moiety, a lactose moiety, azide, fluoride, optionally substituted phenoxy-, optionally substituted pyridinyloxy-, optionally substituted 3-oxo-furanyloxy- of formula (A), optionally substituted 1,3,5-triazinyloxy- of formula (B), 4-methylumbelliferyloxy-group of formula (C), and a group of formula (D) wherein Ra is independently H or alkyl, or two vicinal Ra groups represent a=C(Rb)2 group, wherein Rb is independently H or alkyl, Rc is independently selected from the group consisting of alkoxy, amino, alkylamino and dialkylamino, Rd is selected from the group consisting of H, alkyl and -C(=O)Re, wherein Re is OH, alkoxy, amino, alkylamino, dialkylamino, hydrazino, alkylhydrazino, dialkylhydrazino or trialkylhydrazino, is reacted with an acceptor of formula H-A-R1 or a salt thereof, wherein A and R1 are as defined above, under the catalysis of an enzyme capable of transferring fucose. A compound of formula 1', its use in manufacture of human milk oligosaccharides, a method of manufacture of human milk oligosaccharides, and a fucosyl donor are also provided.

The invention relates to the technical field of pseudomonas aeruginosa detection and discloses a pseudomonas aeruginosa chromogenic culture medium and a method for quick detection by the same. The pseudomonas aeruginosa chromogenic culture medium comprises a basic medium, a chromogenic substrate and an antibacterial agent. The chromogenic substrate comprises 2,3,5-phenyl tetrazolium chloride, 4-methyl umbelliferone-D-glucuronide, and the antibacterial agent comprises nalidixic acid, cycloserine and bile salt. 2,3,5-phenyl tetrazolium chloride acts on a pseudomonas aeruginosa metabolite to showa red color; 4-methyl umbelliferone-D-glucuronide acts on pseudomonas aeruginosa to show fluorescence under a 366nm ultraviolet lamp; by joint action of 2,3,5-phenyl tetrazolium chloride and the antibacterial agent, growth of common disturbance bacteria can be inhibited, and false positive results are avoided. By adoption of the pseudomonas aeruginosa quick detection method, detection and count of pseudomonas aeruginosa can be finished in 24h, and time saving, labor saving, stability, reliability and high sensitivity are realized.

A method is provided for determining the activity of an enzyme which releases methylumbelliferone (MU) from an MU-containing substrate wherein the enzyme has a pH optimum below the pKa of MU comprising: contacting a sample suspected of containing the enzyme with the MU-containing substrate at a pH suitable for activity of the enzyme to allow release of MU by the enzyme; contacting the sample with light of a wavelength in the range of about 310 nm to about 350 nm; determining fluorescence produced by the released MU, thereby determining the activity of the enzyme. This real time method provides improved diagnostic methods, for example for diseases associated with an abnormal level of activity of a glycosidase. The real time assay also can be used to screen compounds for their ability to modulate enzyme activity using MU-containing substrates.

The invention discloses a method for synthesizing various glucosides on a basis of 4-methylumbelliferone. According to the invention, a glycosyl donor peracetyl saccharide and a glycosyl acceptor 4-methylumbelliferone are subjected to a glycosylation reaction under room temperature or under heating with dichloromethane or 1,2-dichloroethane as a solvent and with the combined effect of Lewis acid boron trifluoride ethyl ether and organic alkali triethylamine or pyridine; and protecting groups are removed, such that various glucosides based on 4-methylumbelliferone can be obtained. The glucosides include 4-methylumbelliferone-beta-D-glucopyranosiduronide, 4-methylumbelliferone-beta-D-glucopyranoside, 4-methylumbelliferone-beta-D-xylopyranoside, 4-methylumbelliferone-beta-D-ribofuranoside, 4-methylumbelliferone-alpha-D-galactopyranoside, and 4-methylumbelliferone-alpha-D-mannopyranoside. The method is simple, and can produce a beta or alpha single-configuration target. A glycosylation reaction yield can reach 17-93%.

The invention relates to a growth medium allying a fluorogenic substrate and a pH-sensitive fluorophore, in particular the combination of 4- methylumbelliferone (4-MU) and derivatives of fluorescein. This growth medium is used for the detection by fluorescence of microorganisms by coupling the fluorescence measurements relating to the pH-sensitive fluorophor and the fluorescence measurements relating to the activation of the fluorogenic substrate(s) by the microorganisms.

The invention discloses a reagent and card for detecting organophosphorus and carbamates pesticide residues. Through including 4-methylumbelliferone ester in a molecule with a hydrophobic space, the stability is improved. The 4-methylumbelliferone ester reagent of some examples of the invention can be stably present in an aqueous solution, and can also be hydrolyzed by a reaction with cholinesterase, so that the 4-methylumbelliferone ester reagent can be used as a color developer for the rapid detection of organophosphorus and carbamates pesticide residues.

The invention discloses a biodegradable asynchronous response polymalic acid active deformation material, which relates to the technical field of biomaterials. Polymalic acid, polycaprolactone and 4-methylumbelliferone single-terminated polycaprolactone are used to obtain the polymer through esterification, and then with ultraviolet radiation and stretching fixation process, a kind of The biodegradable polymalic acid active deformation material with asynchronous response has superior performance, simple manufacture and convenient use.

The invention provides a metal-organic framework-based β-glucuronidase probe, the probe substrate of the metal-organic framework-based β-glucuronidase probe can be β-glucuronidase Enzyme-specific catalysis, the probe substrate is 4-methylumbelliferone-β-glucuronide. It can realize ratiometric fluorescence and / or ratiometric colorimetric detection of β-glucuronidase activity. Its detection limit is low, which can reach 0.03U / L; it has good selectivity and is less affected by interference; it can realize high-sensitivity naked-eye visual detection (red to blue); at the same time, it has simple operation, fast response, good precision, High accuracy and other outstanding advantages.

The invention discloses a high-efficiency method for screening salmonella and shigella in enterobacteriaceae, and the method comprises steps of: spreading a specimen on a Mac Conkey agar plate, carrying out incubation at a temperature of 37 DEG C, and selecting a colorless bacterial colony, wherein a portion of the bacterial colony is used for detecting an oxidase activity; inoculating the other portion of the bacterial colony on a filter paper strip soaked with tryptophan, pyroglutamyl-4-methoxy naphthylamines and 4-methylumbelliferone ioxynil octoate, and moistening the inoculated filter paper strip with a drop of water or a drop of buffer; putting the moistened and inoculated filter paper strip in a moisture-maintaining vessel to subject to incubation, and putting the filter paper strip under a Wood lamp to detect fluorescence, wherein fluorescence-positive indicates that a C-8 esterase activity exists; and dropping a drop of a solution containing a diazo dye and iron chloride on the filter paper strip, so that if the filter paper strip shows fluorescence and invariant color, the bacterial colony on the filter paper strip can be identified as salmonella; and if the filter paper strip shows no fluorescence but invariant color, the bacterial colony on the filter paper strip can be identified as shigella. The method of the invention can detect activities of three kinds of enzymes at the same time by employing a method of mixing substrates, the experiment method is simple, a dosage of substrates is lesser, cost is low, and salmonella and shigella can be screened out from nonpathogenic bacteria in enterobacteriaceae efficiently.

The invention provides a 4-methylumbelliferone enrichment material. The enrichment material is prepared by the following method: mixing agarose and a NaOH solution and dimethyl sulfoxide at the mass ratio of agarose to the NaOH solution to dimethyl sulfoxide beign 1:0.2-5:1-10, placing the mixture under the thermostatic waterbath condition, stirring while adding halogenated hydrocarbon or halogenated acid at the mass ratio of agarose to halogenated hydrocarbon or halogenated acid being 1: 0.2-5, continuously stirring after addition and carrying out a thermostatic reaction until the end of thereaction, cleaning with acetone and deionized water, centrifuging and drying to prepare the 4-methylumbelliferone enrichment material. By introducing the material to the bottom of a culture tube, simultaneous determination of Escherichia coli and Escherichia coli can be simultaneously determined in the same culture tube when respective chromogenic / fluorogenic substrate mediums of Escherichia coliand Escherichia coli are added into the culture tube for culture.

The invention discloses an ITO-based iron ion photoelectric sensor and a preparation method thereof, belonging to the technical field of metal ion detection. The ferric ion photoelectric sensor of the invention comprises two parts, a modified fluorescent material and an ITO substrate. By modifying ITO, 4-methylumbelliferone and chitosan-modified fluorescein isothiocyanate materials are cured on the surface of ITO to construct a "one-body dual-purpose" iron ion with both fluorescent and electrochemical properties sensor. Combining the visualization of fluorescence analysis and the advantages of sensitive and rapid electrochemical analysis, the fluorescence characteristics of the electrode are used to realize the visual determination of the concentration of iron ions in water. The results showed that the sensor for Fe 3+ Selective fluorescent recognition at 10‑ 1 ~10- 7 Within the range, the concentration of iron can be judged visually. Fe ion photoelectric sensor of the present invention is to Fe 3+ It also showed good electrochemical detection performance, and the Fe 3+ The detection limit can be as low as 5nM.

The invention discloses a reagent and a card for detecting residues of organophosphorus and carbamate pesticides. The stability of the 4-methylumbelliferyl ketone ester is clathrated in molecules with hydrophobic spaces. The 4-methylumbelliferyl ester reagent of some examples of the present invention can exist stably in an aqueous solution, and can also be hydrolyzed with cholinesterase, which makes it applicable to organophosphorus and amino groups as a color developing agent. Rapid detection of formate pesticide residues.

The invention provides an anti-influenza virus compound. The compound is a benzoic acid derivative, compared with the active compound on the market, the active compound of the anti-influenza virus compound has no chiral center, and the synthesis is simpler; the raw material is simple and easy to obtain, the market supply is more, and the price is low. Through an in-vitro neuraminidase-specific fluorogenic substrate 2'-4-methylumbelliferone-a-N-acetylneuraminic acid test, the anti-influenza virus compound has strong anti-H3N2 influenza activity and strong Anti-H1N1 influenza activity, has better therapeutic effect on influenza B, and is expected to develop anti-influenza virus drugs. The preparation method of the invention is simple, and suitable for industrial production.

The invention discloses a method for synthesizing various glucosides on a basis of 4-methylumbelliferone. According to the invention, a glycosyl donor peracetyl saccharide and a glycosyl acceptor 4-methylumbelliferone are subjected to a glycosylation reaction under room temperature or under heating with dichloromethane or 1,2-dichloroethane as a solvent and with the combined effect of Lewis acid boron trifluoride ethyl ether and organic alkali triethylamine or pyridine; and protecting groups are removed, such that various glucosides based on 4-methylumbelliferone can be obtained. The glucosides include 4-methylumbelliferone-beta-D-glucopyranosiduronide, 4-methylumbelliferone-beta-D-glucopyranoside, 4-methylumbelliferone-beta-D-xylopyranoside, 4-methylumbelliferone-beta-D-ribofuranoside, 4-methylumbelliferone-alpha-D-galactopyranoside, and 4-methylumbelliferone-alpha-D-mannopyranoside. The method is simple, and can produce a beta or alpha single-configuration target. A glycosylation reaction yield can reach 17-93%.