5 results about "Methylumbelliferone" patented technology

The present disclosure provides compositions, methods, and kits for detecting lipolytic activity. In some embodiments, the composition comprises an aqueous assay sample and an organic solvent, wherein the organic solvent comprises 4-methylumbelliferyl oleate (4MuO). Also provided herein are methods for determining the stability of a protein preparation.

The invention relates to the technical field of food biochemistry and lipase detection, in particular to an in-situ screening and identification method of high-activity rice bran endogenous lipase and application of the in-situ screening and identification method. Comprising the following steps: carrying out electrophoretic separation on rice bran protein, incubating separated gel in a solution containing a 4-methylumbelliferone fatty acid ester substrate, and carrying out in-situ identification on a lipase strip in a protein mixture by detecting a fluorescence signal. According to the method, the cross-modal luminescence characteristic of 4-methylumbelliferone in a nucleic acid imaging environment is utilized, and the LC-MS / MS technical means is combined, so that the sequence information of enzyme is retrieved and determined through a bioinformatics database, and the identity attribution of representative lipase in rice bran is defined. The method has the characteristics of rapidness and sensitivity, and lays a foundation for explaining a mechanism of rice bran rancidity caused by rice bran lipase and excavating high-activity lipase.

The application discloses application of glycoside compounds in prevention and treatment of plant pathogenic viruses and belongs to the technical field of pesticide antiviral agents.Taking a tobacco mosaic virus coat protein as an example, small molecules including sugar rings, such as Swertiamarin I (A0) and ZINC85593196 (B0), are screened through virtual screening, molecular dynamics simulation, molecular mechanics and MM / GBSA free energy calculation. Based on this, further optimization is carried out, and six compounds similar to the structure of A0, such as 4-methylumbelliferone-beta-D-xyloside (A1), gardenoside (A2), 4-methylumbelliferone-beta-D-pyranoside (A3), 4-methylumbelliferone-beta-D-pyranoglucoside (A4), catalpol (A5) and esculin (hemihydrate) (A6), are obtained, and in-vivo and in-vitro biological activity evaluation is carried out. The active ingredients screened by the above method have good inhibitory effect on plant viruses.

The invention relates to a method for determining α-arabinofuranosidase (ABF) activity in a sample, referred to as the sample to be analyzed (p), of a product intended for animal feed and containing an additive having α-arabinofuranosidase activity, said α-arabinofuranosidase activity to be determined being specific to said additive, said method comprising the following steps: a sample, referred to as the control sample (c), of said product free from said additive is available on the one hand, and said sample to be analyzed (p) is available on the other hand; for each of said control (c) and sample to be analyzed (p), respectively, an enzymatic fraction having α-arabinofuranosidase activity is extracted under the same conditions, then the α-arabinofuranosidase activity of each of the extracts, respectively ABFc and ABFp, is measured; and ABFp – ABFc is calculated to obtain said ABF activity specific to said additive in said product;according to which method, α-arabinofuranosidase activity is measured by fluorescence using an enzymatic substrate, 4-methylumbelliferyl-α-L-arabinofuranoside (4-MU-ABF), which is hydrolyzable into fluorescent 4-methylumbelliferone (4-MU).

The invention relates to a method for determining α-arabinofuranosidase (abbreviated ABF) activity in a sample, referred to as the sample to be analyzed (p), of a product intended for animal feed and containing an additive that exhibits ABF activity, the ABF activity to be determined being specific to the additive, the method comprising the following steps: on the one hand, a sample, referred to as the control sample (c), of the product which is free of the additive, and, on the other hand, the sample to be analyzed (p), are provided; for each of the control sample (c) and the sample to be analyzed (p), respectively, an enzyme fraction exhibiting ABF activity is extracted under the same conditions, then the ABF activity of each of the extracts, respectively ABFc and ABFp, is measured; and ABFp - ABFc is calculated to obtain the specific ABF activity of the additive in the product; according to which method the ABF activity is measured by fluorescence by means of a substrate for the enzyme, 4-methylumbelliferyl-α-L-arabinofuranoside, which is hydrolyzable to fluorescent 4-methylumbelliferone.