Paper-based detection device for detecting cronobacter and application of paper-based detection device

Abstract

The invention discloses a paper-based detection device for detecting cronobacter and application of the paper-based detection device, belonging to the technical field of food safety. In order to solve the problems existing in processes of detecting cronobacter by using a traditional microbiological method and a molecular biological method, the invention provides the paper-based detection device, and the detection principle of the paper-based detection device is that the cronobacter can generate intracellular enzyme alpha-glucosidase after ultrasonication treatment, a specific enzyme-substrate reaction can be carried out on the paper-based detection device modified by the substrate X(alpha)lc, and then the cronobacter can be quickly and visually qualified and quantified on the paper-based detection device. The paper-based detection device disclosed by the invention has the characteristics of simple manufacturing method, low cost, high device sensitivity and the like, and can be used for detecting cronobacter pollution in food and food processing processes in real time, especially cronobacter pollution in production of infant formula milk powder.

Description

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AI Technical Summary

Benefits of technology

This patented method allows crookbial cells called Coryneforma coccoides (Croc), or other microorganisms like Bacillus subtilis, producing an enzymatic substance known as alpha glucan lyases, specifically labeled beta gluconolactonase, this modification being able to bind certain sugars such as xylitol molecules on proteins found naturally within these organism's cell walls. These modifications make it difficult for harmful germs from animals to infect humans through ingestion. By measuring changes caused by gene expression during fermentation, researchers have been able to identify potentially dangerous strain(s). Additionally, there was also some evidence showing how long ago they were alive when exposed to different types of chemical agents. Overall, this technique offers technical benefits over existing techniques but at least one advantage due to its simplicity, ease of manufacture, fast response times, and accurate results compared to current technologies.

Problems solved by technology

This patents discusses various ways to detect harmful organisms like crenasiruses by measuring enzymatic activity called alpha glucanases. However, current assays require long periods before they become visible signs indicating deteriorating levels of biosurfactants. There exists a problem where conventional chemical testing requires complex procedures and equipment. Immune sensory tests offer better accuracy than existing diagnoses while DNA probings costly and requiring expertise operation.

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