Rapid detection kit for Cronobacter sakazakii

Abstract

The invention discloses a rapid detection kit for Cronobacter sakazakii. The kit comprises a dry powder detection reagent, reconstitution fluid, positive control dry powder, negative control dry powder, confining liquid, a developing solution and a lysis solution. Six primers in the kit correspond to eight sectors of a specific target gene sequence of the Cronobacter sakazakii; by adding the developing solution, the negative and positive developing difference is obvious, and the rapid detection kit has the advantages of high specificity, high sensitivity, easiness in operation, simple result judgment and the like and is suitable for rapid detection of the Cronobacter sakazakii in the field of food safety and sanitation. Besides, the rapid detection kit is a dry powder detection reagent, isconvenient to transport at normal temperature and is simple and convenient to use; no special instrument is needed, so that the cost is reduced and application range of a product is widened; the rapid detection kit is more suitable for rapid detection of the Cronobacter sakazakii in field and basic units of remote regions and has broad application prospect.

Description

technical field [0001] The invention relates to a rapid detection kit for microorganisms, in particular to a rapid detection kit for Cronobacter sakazakii. Background technique [0002] Cronobacter sakazakii (Cronobacters akazakii) is a Gram-negative non-bacillus, which has the characteristics of flagella, motility, and yellow pigment production, and belongs to the Cronobacter genus (Cronobacter). Cronobacter sakazakii is a common food-borne opportunistic pathogen, which has been listed by the International Commission on Microbiological Specifications for Foods (ICMSF) as "seriously endangering specific populations, endangering life or chronic substantial sequelae or It is a kind of pathogenic bacteria with long-term effects, which is widely distributed in the body and natural environment, mainly infecting infants and young children and causing serious diseases such as necrotizing enterocolitis, meningitis and sepsis, especially for those with low immunity and high body weig...

AI Technical Summary

Problems solved by technology

In routine operations, it is necessary to prepare the corresponding reaction system solution and then perform amplification. Multiple operations are likely to cause deviations in results and cross-contamination in the experimental area. Some reagents required for detection need to be frozen and stored, which is not suitable for high temperature and long distance Transportation, especially Bst DNA polymerase, needs to be stored at -20°C, and repeated freezing and thawing should not be used to avoid affecting the enzyme activity, which will increase the transportation cost of the reagent

At present, this detection method is a non-standard method, which has not been promoted in the fields of food safety and medicine. The existing LAMP products are only aimed at the detection process, and there is no mention of the method and method of sample pretreatment. In addition, the market based on LAMP There are fewer rapid detection kits for Cronobacter sakazakii, and similar dry powder products that can be transported at room temperature have not yet appeared

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