18 results about "Cronobacter sakazakii" patented technology

The invention relates to the field of microorganisms, and discloses lactobacillus delbrueckii subsp. Bulgaricus, a fungicide and an additive containing the lactobacillus delbrueckii subsp. Bulgaricus, application of the fungicide and the additive and a pollution relieving method. The preservation number of the lactobacillus delbrueckii subsp. Bulgaricus is CCTCC (China Center For Type Culture Collection) NO: M 20251218, and the microbial agent comprises the lactobacillus delbrueckii subsp. Bulgaricus, the lactobacillus delbrueckii subsp. Bulgaricus and the microbial inoculum provided by the invention can be used for preparing food additives for preventing and / or relieving microplastic-induced body injury, reducing cadmium pollution of food and inhibiting food-borne enterobacter sakazakii, can provide a new scheme for infant food safety and health protection, and have relatively high application value.

The invention relates to the technical field of biology, and particularly discloses a cronobacter sakazakii nucleic acid aptamer and application thereof in preparation of a biosensor. According to the invention, a nucleic acid aptamer with a DNA sequence as shown in SEQ ID No: 1 is designed for Cronobacter sakazakii MipA protein, and 5'terminal amino is modified. The aptamer can be used for constructing a fluorescence quenching type biosensor, and comprises a fluorescent probe (fluorescent nanoparticles synthesized by citric acid diammonium, thiourea, tetramethyl orthosilicate and cerium dioxide, and a first aptamer modified by carboxyl) and a quenching probe (citric acid reduced gold nanoparticles, a second aptamer modified by sulfydryl, SEQ ID No: 2). Compared with a traditional method, the screening efficiency is remarkably improved, and the detection time is lt; the method has the advantages of high sensitivity, high interference resistance and affinity constant of 13.28 nM, is suitable for rapid monitoring of infant food, and reduces the economic cost by at least 40%.

The invention belongs to the technical field of agricultural microorganism development and application industry, and particularly relates to a high-temperature-resistant cronobacter sakazakii strain with cellulose degradation capacity and application. According to the specific technical scheme, the high-temperature-resistant cronobacter sakazakii strain with the cellulose degradation capacity is named as cronobacter sakazakii, and the 16S rRNA nucleotide sequence of the cronobacter sakazakii strain is as shown in SEQ ID NO: 1; the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.33826, and the preservation date is March 14, 2025. The Cronobacter sakazakii disclosed by the invention can be used for efficiently degrading cellulose, hemicellulose and acidic detergent lignin, and a new solution is provided for the feed pretreatment of straws and dregs, the solving of agricultural non-point source pollution caused by the straws and the acceleration of high-temperature composting. Moreover, the strain still keeps high enzymatic activity at a high temperature of 65 DEG C, so that the development of related products of a microbial agent of the strain is greatly facilitated, the application range of the strain is expanded, and the strain has a wide application prospect.

The invention discloses a Cronobacter sakazakii bacteriophage Csp-TJDF02 and an application of the Cronobacter sakazakii bacteriophage The phage is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20252611. The bacteriophage belongs to the muscular tail bacteriophage family, the whole length of a genome is 143, 084 bp, and the bacteriophage does not contain virulence genes and drug-resistant genes and has high biological safety. The Csp-TJDF02 has excellent lysis performance, the optimal infection complex number is as low as 0.001, the incubation period is short (10 min), and the Csp-TJDF02 has good pH stability and heat resistance. Particularly, the bacteriophage can still keep efficient antibacterial activity in a high-protein milk powder matrix and can remarkably inhibit formation of a bacterial biofilm. The bacteriophage provided by the invention can be prepared into a cleaning solution, a spraying agent or a biological bacteriostatic agent, is used for preventing and controlling cronobacter sakazakii in a medical environment, a food processing environment and a food matrix, and effectively reduces infection risk and quality deterioration.

This invention relates to an aptamer of *Cronobacter sakazakii* and a method for preparing a fluorescence-quenched biosensor thereof, belonging to the field of biosensor technology. The DNA sequence of the *Cronobacter sakazakii* aptamer is shown in SEQ ID No:1. Experiments show that the cross-reactivity of this aptamer with common foodborne pathogens such as *Staphylococcus aureus* and *Listeria monocytogenes* is less than 25%. The construction process of the fluorescence-quenched biosensor based on the *Cronobacter sakazakii* aptamer includes the mixing of activating agents, the preparation of fluorescent probes and quenching probes, and the mixing of fluorescent probes and quenching probes in a volume ratio to obtain a sensitive and specific fluorescence-quenched biosensor. The fluorescence-quenched biosensor of this invention exhibits high linearity and low detection limits; simultaneously, the screening method is simple and convenient, requiring only the use of an existing Autodoc server for rapid screening.

The invention relates to a cronobacter sakazakii aptamer and a preparation method of a fluorescence quenching type biosensor of the cronobacter sakazakii aptamer, and belongs to the technical field of biosensors. The DNA sequence of the Cronobacter sakazakii aptamer is as shown in SEQ ID No: 1, and experiments show that the cross reaction rates of the aptamer to common food-borne pathogenic bacteria such as staphylococcus aureus and listeria monocytogenes are all lower than 25%. The construction process of the fluorescence quenching type biosensor based on the Cronobacter sakazakii aptamer comprises the working procedures of mixing activation reactants and preparing a fluorescence probe and a quenching probe, and the sensitive and specific fluorescence quenching type biosensor is prepared by mixing the fluorescence probe and the quenching probe according to the volume ratio. The fluorescence quenching type biosensor disclosed by the invention has a relatively high linear fitting degree and a relatively low detection limit; meanwhile, the screening method is simple and convenient, and rapid screening can be achieved only through an existing AUTODOCK server.

This invention discloses a quadruple Taqman qPCR detection reagent for detecting Cronobacter sakazakii, Cronobacter malondialdehyde, Cronobacter zurense, and Cronobacter dublin. The reagent includes specific primers and Taqman probes corresponding to these four Cronobacter sakazakii, Cronobacter malondialdehyde, Cronobacter zurense, and Cronobacter dublin. The primer-probe combination targeting Cronobacter sakazakii, Cronobacter malondialdehyde, Cronobacter zurense, and Cronobacter dublin provided by this invention has extremely high specificity and sensitivity, providing a reliable basis for the control of important Cronobacter species, effectively reducing the workload of single-pair qPCR detection, significantly improving work efficiency, and reducing economic costs.

The invention discloses screening of a Cronobacter sakazakii aptamer and development of a functional nucleic acid double-recognition element biosensor. The screening comprises the following steps: (1) determining a sequence of the Cronobacter sakazakii aptamer; (2) optimizing reaction conditions of the cronobacter sakazakii biosensor; and (3) carrying out visual detection on the cronobacter sakazakii. The detection principle of the sensor is as follows: an aptamer is coupled to a carboxyl magnetic bead to serve as a capture element, enrichment of target bacterial cells is realized through magnetic separation, and a large amount of dissolved contents provide a target with a higher order of magnitude for ribozyme cutting by splitting the target bacterial cells. Short-chain nucleic acid generated after ribozyme cutting is used as a primer of RCA amplification reaction, generation of a product with a large number of G-quadruplex sequences can be triggered, and finally, TMB is catalyzed to generate chromogenic reaction by utilizing peroxidase-like activity of a G-quadruplex / hemin compound, so that visual output of signals can be realized, and ultra-sensitive detection of cronobacter sakazakii is completed.

The application relates to the field of biotechnology, and discloses a Cronobacter sakazakii phage Csp-TJDF01 and application thereof. The phage has been preserved in the China Center for Type Culture Collection (CCTCC) on November 20, 2025, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20252610, the full length of the genome is 143081 bp, and the phage does not carry virulence and drug resistance genes. The phage has high efficient lytic activity on the Cronobacter sakazakii, can significantly inhibit the formation of a biofilm of the Cronobacter sakazakii on a solid surface, and can effectively inhibit bacterial growth in a food matrix such as infant formula milk powder. The phage has high safety, is suitable for preparing bacteriostatic agents or killing agents, and is used for pathogenic bacteria control in medical and food environments.

The application discloses a broad-spectrum bacteriophage targeting mucoid Escherichia coli, Cronobacter and Salmonella and application thereof Escherichia coli phage vB EcoP 3BB7B, the bacteriophage can specifically lyse colanic acid-producing mucoid Escherichia coli, and breaks through the technical bottleneck that a mucous layer limits adsorption of most bacteriophages; further has cross-species lysis capacity, not only has good lysis activity on mucoid Escherichia coli, but also can effectively lyse important foodborne pathogenic bacteria such as Salmonella of various serotypes and Cronobacter sakazakii, and significantly widens the host spectrum range. The bacteriophage can realize efficient amplification under the condition of a lower infection multiple, has good environmental stability, has strong lysis activity, does not carry drug resistance genes and virulence-related genes, has high safety, and has a good application prospect in clinical and food safety prevention and control.

The application belongs to the field of microorganism molecule detection, and discloses a multiplex qPCR kit and method for simultaneously detecting multiple pathogenic bacteria. rfbE The multiplex qPCR primer probe group obtained by optimizing and designing the specific gene fragments of the genomes of Salmonella, Listeria monocytogenes and Cronobacter sakazakii and the high-specificity gene of Escherichia coli O157:H7 has high specificity, and only the four target bacteria appear fluorescence signals in the specific fluorescence signal channel, so that the detection result is more accurate. The multiplex qPCR detection kit can complete high-sensitivity detection of the four pathogenic bacteria in a single tube within 10 hours, wherein the whole qPCR process only needs about 1 hour, the detection sensitivity can be as low as 1.9-12 CFU / Test, the detection efficiency is significantly improved, the detection cost is reduced, and the multiplex qPCR kit is particularly suitable for large-scale sample investigation and actual detection.

The invention discloses a Cronobacter sakazakii specific outer membrane protein gene target based on RPA-CRISPR, a primer, a detection system and a detection method. According to the present invention, the target sequence, the RPA primer and the crRNA have good inter-genus specificity, and the constructed detection method has no specific recognition reaction on other genus species, has a certain broad spectrum on other Cronobacter species of the same genus, and has accurate and reliable detection results. Besides, the detection method is combined with a boiling method or a kit to extract sample DNA, and on the premise that thallus enrichment is not carried out, the detection sensitivity of the cronobacter sakazakii genome DNA is 10 <-4 > ng / mu L, the target sensitivity is 1.43 copies / mu L, and the bacterial liquid sensitivity is 6 CFU / mL. The method is simple in operation, high in sensitivity and reliability and suitable for on-site rapid detection of cronobacter sakazakii in related supervision departments when standard detection is carried out on infant formula milk powder and goat milk powder, the sample detection limit reaches 100 CFU / mL, and the method is simple in sample pretreatment, convenient to operate, high in sensitivity and high in reliability and is suitable for on-site rapid detection of cronobacter sakazakii in related supervision departments.

Provided are a Cronobacter phage, a liquid formulation thereof, and use thereof in the prevention and control of Cronobacter sakazakii. The provided phage is Cronobacter phage vB_CsaM_2375H2, which is a novel phage belonging to Pseudotevenvirus and has an accession number of CCTCC NO: M 2024570. The phage vB_CsaM_2375H2 has good stability at pH 3-11 and a temperature of 25-70 °C and does not carry any virulence or antibiotic resistance gene, meeting the safety requirements in actual applications. The phage vB_CsaM_2375H2 specifically lyses Cronobacter sakazakii and can remove the biofilm of Cronobacter sakazakii, with a biofilm removal rate of up to 62.55%.

The present invention provides a kit for joint detection of eight foodborne pathogenic bacteria based on the multiplex probe melting curve method, belonging to the technical field of microbial detection. The primer-probe Mix in the kit provided by the present invention includes primers shown in SEQ ID NO.1-16, probes shown in SEQ ID NO.17-24, and substrates shown in SEQ ID NO.25-32. The present invention uses 2 fluorescence channels and differentiates them through different Tm temperature peaks of substrate melting peaks in a single fluorescence channel. The present invention realizes the full detection of characteristic genes in 8 foodborne pathogenic bacteria (Salmonella, Staphylococcus aureus, Escherichia coli O157, Listeria monocytogenes, Vibrio, Yersinia enterocolitica, Cronobacter sakazakii, Shigella) in a single-tube fluorescence quantitative PCR reaction, and has the advantages of simple operation, good specificity, high sensitivity and low cost.

Provided are an isoenzyme aroG mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase and the use thereof. The mutant is obtained by means of mutation of aroG derived from Escherichia coli, Klebsiella aerogenes, Proteus mirabilis, Yersinia kristensenii or Cronobacter sakazakii. The mutant can increase the yield of tryptophan in gene-edited engineered bacteria, and has a great application value.

The invention discloses screening of cronobacter sakazakii deoxyribozyme and development of a fluorescence sensor. The screening comprises the following steps: (1) determining a sequence of the cronobacter sakazakii deoxyribozyme; and (2) detecting the cronobacter sakazakii. The detection principle of the sensor is as follows: a fluorophore and a quenching group are respectively modified at two ends of an RNA substrate. When the reaction does not occur, the distance between the fluorophore and the quenching group is close, due to the fluorescence resonance energy transfer effect, fluorescence emitted by the fluorophore is absorbed by the quenching group, the system is in a closed state, and no fluorescence signal is generated. When a target molecule exists, the catalytic activity of deoxynucleic acid can be activated, so that the deoxynucleic acid can specifically cut an RNA substrate. After cutting, the fluorophore and the quenching group are physically separated, the fluorophore can freely emit fluorescence, and the system is turned into an open state. By detecting the intensity change of a fluorescence signal when the excitation wavelength is 490 nm and the emission wavelength is 520 nm, the detection of target molecules can be realized.

The present application relates to the single fluorescence method and double fluorescence method for rapid detection of Cronobacter sakazakii and Cronobacter zurichensis by using ERA technology. The present application also relates to the oligonucleotide primer probe composition for the method. The present application also relates to the detection kit comprising the composition. The ERA fluorescence method detection using the composition of the present application can simply, rapidly, specifically and sensitively detect Cronobacter sakazakii and Cronobacter zurichensis.

The present disclosure relates to a novel bacteriophage having specific antibacterial activity against Cronobacter sakazakii and a method for preparing an encapsulated bacteriophage powder. The novel bacteriophage of the present disclosure exhibits high specificity for Cronobacter sakazakii, excellent lytic activity, rapid and sustained antimicrobial activity, and superior stability with respect to temperature and pH. In addition, according to the present disclosure, by encapsulating the bacteriophage using collagen peptide, the survivability of the bacteriophage can be improved. The encapsulated bacteriophage thus prepared remains stably viable even after being converted into powder form, maintains excellent antimicrobial activity, and offers enhanced convenience in distribution and use, thereby demonstrating high industrial applicability.