6 results about "Cronobacter sakazakii" patented technology

The invention discloses a Cronobacter sakazakii bacteriophage Csp-TJDF02 and an application of the Cronobacter sakazakii bacteriophage The phage is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20252611. The bacteriophage belongs to the muscular tail bacteriophage family, the whole length of a genome is 143, 084 bp, and the bacteriophage does not contain virulence genes and drug-resistant genes and has high biological safety. The Csp-TJDF02 has excellent lysis performance, the optimal infection complex number is as low as 0.001, the incubation period is short (10 min), and the Csp-TJDF02 has good pH stability and heat resistance. Particularly, the bacteriophage can still keep efficient antibacterial activity in a high-protein milk powder matrix and can remarkably inhibit formation of a bacterial biofilm. The bacteriophage provided by the invention can be prepared into a cleaning solution, a spraying agent or a biological bacteriostatic agent, is used for preventing and controlling cronobacter sakazakii in a medical environment, a food processing environment and a food matrix, and effectively reduces infection risk and quality deterioration.

This invention relates to an aptamer of *Cronobacter sakazakii* and a method for preparing a fluorescence-quenched biosensor thereof, belonging to the field of biosensor technology. The DNA sequence of the *Cronobacter sakazakii* aptamer is shown in SEQ ID No:1. Experiments show that the cross-reactivity of this aptamer with common foodborne pathogens such as *Staphylococcus aureus* and *Listeria monocytogenes* is less than 25%. The construction process of the fluorescence-quenched biosensor based on the *Cronobacter sakazakii* aptamer includes the mixing of activating agents, the preparation of fluorescent probes and quenching probes, and the mixing of fluorescent probes and quenching probes in a volume ratio to obtain a sensitive and specific fluorescence-quenched biosensor. The fluorescence-quenched biosensor of this invention exhibits high linearity and low detection limits; simultaneously, the screening method is simple and convenient, requiring only the use of an existing Autodoc server for rapid screening.

This invention discloses a quadruple Taqman qPCR detection reagent for detecting Cronobacter sakazakii, Cronobacter malondialdehyde, Cronobacter zurense, and Cronobacter dublin. The reagent includes specific primers and Taqman probes corresponding to these four Cronobacter sakazakii, Cronobacter malondialdehyde, Cronobacter zurense, and Cronobacter dublin. The primer-probe combination targeting Cronobacter sakazakii, Cronobacter malondialdehyde, Cronobacter zurense, and Cronobacter dublin provided by this invention has extremely high specificity and sensitivity, providing a reliable basis for the control of important Cronobacter species, effectively reducing the workload of single-pair qPCR detection, significantly improving work efficiency, and reducing economic costs.

The application relates to the field of biotechnology, and discloses a Cronobacter sakazakii phage Csp-TJDF01 and application thereof. The phage has been preserved in the China Center for Type Culture Collection (CCTCC) on November 20, 2025, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20252610, the full length of the genome is 143081 bp, and the phage does not carry virulence and drug resistance genes. The phage has high efficient lytic activity on the Cronobacter sakazakii, can significantly inhibit the formation of a biofilm of the Cronobacter sakazakii on a solid surface, and can effectively inhibit bacterial growth in a food matrix such as infant formula milk powder. The phage has high safety, is suitable for preparing bacteriostatic agents or killing agents, and is used for pathogenic bacteria control in medical and food environments.

The application discloses a broad-spectrum bacteriophage targeting mucoid Escherichia coli, Cronobacter and Salmonella and application thereof Escherichia coli phage vB EcoP 3BB7B, the bacteriophage can specifically lyse colanic acid-producing mucoid Escherichia coli, and breaks through the technical bottleneck that a mucous layer limits adsorption of most bacteriophages; further has cross-species lysis capacity, not only has good lysis activity on mucoid Escherichia coli, but also can effectively lyse important foodborne pathogenic bacteria such as Salmonella of various serotypes and Cronobacter sakazakii, and significantly widens the host spectrum range. The bacteriophage can realize efficient amplification under the condition of a lower infection multiple, has good environmental stability, has strong lysis activity, does not carry drug resistance genes and virulence-related genes, has high safety, and has a good application prospect in clinical and food safety prevention and control.

The present application relates to the single fluorescence method and double fluorescence method for rapid detection of Cronobacter sakazakii and Cronobacter zurichensis by using ERA technology. The present application also relates to the oligonucleotide primer probe composition for the method. The present application also relates to the detection kit comprising the composition. The ERA fluorescence method detection using the composition of the present application can simply, rapidly, specifically and sensitively detect Cronobacter sakazakii and Cronobacter zurichensis.