25 results about "Bacterial dna" patented technology

The present invention proposes a method for evaluating the growth of exogenous microorganisms in soil, comprising the following steps: S1, adding exogenous microorganisms and H2 to dry soil 18 O to carry out soil culture test, collect soil samples and extract soil DNA; S2, H2 16 O and H2 18 O. Preparing a liquid culture medium for culturing exogenous microorganisms, collecting the obtained exogenous microorganisms and extracting DNA; S3. Dissolving the soil DNA obtained in S1 and the bacterial DNA sample in S2 in a cesium chloride solution and performing density gradient centrifugation to obtain the distribution ratios of different DNAs along the density gradient; S4. Determining the average density of the exogenous microbial DNA in the soil based on the obtained distribution ratios of the different DNAs to achieve a quantitative evaluation of the growth degree of the exogenous microorganisms; the present invention performs quantitative PCR analysis on unique gene fragments of exogenous microorganisms, which can eliminate the interference of other microorganisms in the soil and perform highly specific analysis of the exogenous microorganisms.

This invention provides a detection method for distinguishing between live and dead bacteria of the watermelon fruit spot bacterial disease fungus. [Solution] A detection method for distinguishing between live and dead bacteria based on PMA-PCR technology, comprising: a step of collecting and suspending bacteria in a sample to be detected, which involves suspending the sample in physiological saline to obtain a bacterial suspension and heating it in a 100°C water bath for 10 minutes to prepare a dead bacterial suspension; a step of PMA treatment, which involves adding propidium monoazide (PMA) to the bacterial suspension; a step of blue light crosslinking, which involves irradiating the PMA-treated bacterial suspension with blue light to activate the PMA and covalently bind it to the dead bacterial DNA; a step of DNA extraction, which involves collecting bacterial cells by centrifugation and extracting DNA using a kit method; a PCR amplification step, which involves amplification using a specific primer pair; and a step of detecting the amplified product by agarose gel electrophoresis, which involves determining the result, which involves a step of detecting the product by agarose gel electrophoresis, wherein a target band appears in the live bacterial sample and no band appears in the dead bacterial sample.

The cat litter basin comprises a cat litter rotating basin, a cat litter basin main body and an excrement collecting bin, the cat litter rotating basin is arranged on the cat litter basin main body, the excrement collecting bin is arranged below the cat litter basin main body, an excrement collecting window is formed in the cat litter basin main body, and the excrement collecting window is arranged below the cat litter basin main body. A positive ion generation assembly, a negative ion generation assembly, a high-energy ion generation piece and a flow guide fan are arranged in the cat litter basin body. Through cooperation of the positive ion generating assembly and the negative ion generating assembly, a plasma state can be generated in the excrement collecting bin, peculiar smell molecules in air are disintegrated in the process of forming the plasma state, meanwhile, plasmas can break down cell walls of bacteria, viruses, microorganisms and the like attached to pet excrement, the reproductive capacity of DNA chains of the bacteria is blocked and reduced, and therefore the pet excrement collecting effect is improved. Meanwhile, through the arrangement of the high-energy ion generating part, high-energy ions can effectively decompose stubborn odor molecules in the excrement collecting bin, and the use experience of a user and the competitiveness of a product are effectively improved by adopting a consumable-free and low-power-consumption deodorization mode.

This application relates to a method, apparatus, computer device, computer-readable storage medium, and computer program product for bacterial phenotypic prediction based on bacterial DNA, which can be used in the field of bioinformatics. The method includes: constructing a heterogeneous graph; the heterogeneous graph includes nodes and the connections between nodes; performing semantic training processing on mutation embedding information based on the heterogeneous graph to obtain mutation semantic embedding information of the mutation embedding information; determining the target mutation semantic embedding information corresponding to the mutation information in the input sample based on the mutation semantic embedding information; performing aggregation processing on the target mutation semantic embedding information to obtain aggregated feature information of the target mutation semantic embedding information; inputting the aggregated feature information into a preset drug resistance prediction model for drug resistance prediction processing to obtain the drug resistance prediction result of the input sample against anti-tuberculosis drugs. This method can improve the efficiency of drug resistance prediction.

The invention discloses a sampling chip and a sampling method for DNA of bacteria. The chip is composed of a bottom plate, a middle plate and an upper plate which are integrated through thermocompression bonding, a reaction cavity, a liquid storage cavity, a waste liquid cavity and microfluidic channels which are communicated with one another are sequentially arranged on the bottom plate, paraffin valves capable of being controlled by heating are arranged in the channels, and heating pieces are arranged at the bottom of the reaction cavity. According to the chip, a gas source is introduced through a gas inlet, and the steps of sample loading, cell lysis, supernate removal, ethanol cleaning, pneumatic drying, DNA elution and the like are realized in a closed flow path. The method comprises the following steps: loading and sealing a bacterium-containing sample, heating, splitting and combining, discharging supernatant under the driving of a gas source, introducing a cleaning solution, heating and volatilizing residual liquid, injecting an eluent, desorbing DNA at a constant temperature, and finally collecting the eluent. According to the scheme, extraction of bacterial DNA is completed in a single chip, dependence on external equipment such as centrifugation and pipetting is reduced, and a DNA sample which can be directly used for downstream amplification or sequencing analysis is obtained.

An approach for differentially isolating eukaryotic (plant and animal) DNA from bacterial DNA prior to sequencing using a combination of size exclusion-based separation and differential cell lysis. The method of the present invention exploits the differences of the cellular size and components of each type of organism to be separated. The composition and nature of the cell wall of plant cells, enzymatic sensitivity of bacterial and animal cells and overall size difference of bacterial and plant / animal cells allows one portion of a mixed sample to be lysed while retaining the integrity of the remaining organisms. Separation of one phylogenetic component then permits the remaining components to be extracted with minimal contribution from the preceding component. The separation of DNAs from differing contributing kingdoms in an unknown sample increases interpretability through decreasing complexity in subsequent sequencing applications.

The application discloses a bacteria with limited reproduction capacity, a method for controlling bacterial reproduction and application, and belongs to the technical field of biotechnology and cell immunotherapy. The bacterial DNA comprises an unnatural amino acid orthogonal translation module, a suicide module and a rescue module. Based on the unnatural amino acid (ncAA) orthogonal translation system, the orthogonal genetic barrier theory is used, gene circuit design and element modularization are used, bacterial reproduction generations are accurately controlled, bacterial growth is effectively controlled, and the bacteria are prevented from escaping, and the bacteria have wide applicability. The bacteria and the method have wide application prospects in the development of attenuated live vaccines.

The invention discloses a thermophilic thermophilic bacteria fermentation product, a preparation method and application thereof and an oil control product, and belongs to the technical field of cosmetics. Thermophilic thermophilic bacteria are cultured in a first-stage seed solution culture medium and a second-stage seed solution culture medium in sequence, the thermophilic thermophilic bacteria are promoted to the logarithmic phase, then a mulberry leaf extracting solution is added for fermentation, and the oil control product is obtained. According to the method, the growth stability of thalli is effectively prolonged, the stability of a fermentation microenvironment is maintained, the metabolic activity of the thermophilic thermophilic bacteria is improved, normal replication of thallus DNA at high temperature can be guaranteed, and the thermophilic thermophilic bacteria can grow at a high speed in the whole growth period. According to the method disclosed by the invention, the accumulation of byproducts can be avoided, active ingredients with an oil control effect in the thermophilic thermophilic bacteria are effectively enriched, and a thermophilic thermophilic bacteria fermentation product which has the effects of improving the mRNA expression quantity of AMPK, effectively inhibiting the activity of 5 alpha-reductase, inhibiting sebaceous gland cells from synthesizing lipid droplets and reducing hormone-induced lipid synthesis is obtained; the oil control activity is excellent.

To provide nucleic acids, such as low-toxicity vectors, that can be stably amplified in the host; to provide genome editing complexes encoded by the nucleic acids; and to provide a genome editing method that uses the vectors and, if necessary, nucleic acid modification enzymes to modify bacterial DNA without relying on host-dependent factors such as RecA, is applicable to a wide range of bacteria, while suppressing nonspecific mutations.SOLUTION: The present invention provides a complex in which a nucleic acid sequence recognition module that specifically binds to a target nucleotide sequence in double-stranded DNA is bound to a proteolytic tag consisting of: (i) a peptide containing three hydrophobic amino acid residues at the C-terminus; or (ii) a peptide containing three amino acid residues in which at least some of the amino acid residues have been substituted with serine, at the C-terminus.SELECTED DRAWING: None

Methods are provided for detecting disease in liquid biopsy samples by identifying microbial rRNA. The methods include isolating nucleic acids from extracellular vesicles, treating the nucleic acids with DNase to remove DNA including human cfDNA, mcfDNA, and contaminating microbial DNA, and then amplifying a region of a microbial phylogenetic rRNA. To further reduce contaminating DNA, the amplifying step can include reverse transcription to translate the phylogenetic region into cDNA and extend the cDNA ends with a T7 promoter and sequencing adaptors. The translated region is amplified with primers to the extended ends of the cDNA and sequenced. The resulting phylogenetic microbial sequences are useful for diagnosis and monitoring of diseases including cancer. The method can further include amplification of other human or bacterial DNA or RNA sequences to augment disease diagnosis. The method results in a very low contribution of contaminating microbial DNA and RNA to the overall microbial signature.

The invention relates to a bacterial phenotype prediction method and device based on bacterial DNA, computer equipment, a computer readable storage medium and a computer program product, and can be applied to the technical field of biological information. The method comprises the following steps: constructing a heterogeneous graph; the heterogeneous graph comprises nodes and a connection relationship between the nodes; performing semantic training processing on the mutation embedding information according to the heterogeneous graph to obtain mutation semantic embedding information of the mutation embedding information; according to the mutation semantic embedding information, determining target mutation semantic embedding information corresponding to mutation information in the input sample; performing aggregation processing on the target mutation semantic embedding information to obtain aggregation feature information of the target mutation semantic embedding information; and inputting the aggregated feature information into a preset drug resistance prediction model for drug resistance prediction processing to obtain a drug resistance prediction result of the input sample on the anti-tuberculosis drug. By adopting the method, the drug resistance prediction efficiency can be improved.

The application discloses a bacterial fluorescence detection method based on asymmetric RPA and hydrogel. The bacterial detection sample, asymmetric RPA reagent, molecular beacon, lysozyme and hydrogel monomer are jointly added into a chip groove to form a hydrogel; the lysozyme first cracks the bacteria and releases DNA, and then the RPA is used for asymmetric amplification to generate a single-stranded amplicon; the single-stranded amplicon is hybridized with the molecular beacon to restore fluorescence, and the hydrogel network restricts the diffusion of bacterial DNA, single-stranded amplicon and hybridization chain, so that the fluorescence hybridization chain forms an aggregation point near the bacteria; the aggregation points are counted by a fluorescence microscope to obtain the number of target bacteria. The application cracks the bacteria and performs asymmetric isothermal amplification in the hydrogel in situ, shortens the detection time, and does not need long bacterial culture and DNA purification steps and complex instruments or professional technical operation. The system is stable, easy to carry, and suitable for rapid detection in a resource-limited scene.

This invention relates to the field of biomedical technology, specifically disclosing a biomimetic bacterial DNA nanomicelle, its preparation method, and its applications. By mimicking the structural and functional characteristics formed when microorganisms infect a host, exogenous nucleic acid stimulation signals and oxidative stress regulation functions are integrated into a single nanostructure. A micellar nanostructure is designed, comprising a complex of oligonucleotides containing CpG motifs and ferrocene groups, which is formed through self-assembly in solution. This nanomicelle can significantly promote the proliferation or differentiation of dendritic cells, improve their maturity, and enhance their immune activation capacity; by enhancing the cross-presentation capacity of dendritic cells to target antigens, it effectively promotes cytotoxic CD8+. + T-cell immune responses; it can also enhance the body's cellular immune response by inducing the activation and proliferation of antigen-specific cells. These DNA nanomicelles have significant clinical application value in areas such as vaccine adjuvants, tumor immunotherapy, and immune regulation.

The subject invention provides fluorophore-quencher nucleic acid molecules comprising relaxed or supercoiled DNA molecules, and their use in rapid and efficient high-throughput screening (HTS) assays to screen and identify compounds that inhibit DNA gyrases. These compounds can be used as antibiotics for treating bacterial infections, especially, multidrug resistant bacterial infections.

The invention relates to the technical field of films, in particular to a preparation method of a polylactic acid antibacterial film. The polylactic acid antibacterial film is prepared from the following raw materials in parts by weight: 80-95 parts of a polylactic acid base material, 3-10 parts of a first antibacterial material, 2-8 parts of a second antibacterial material, 4-12 parts of a first reinforcing material, 2-5 parts of a second reinforcing material and 1-3 parts of a dispersing aid. According to the preparation method, phenolic acid molecules penetrate through microbial cell membranes through a hydrophobic effect to destroy membrane integrity, flavone components are combined with bacterial DNA topoisomerase to inhibit nucleic acid replication, and the two substances generate a synergistic effect through a pi-pi conjugation effect, so that the antibacterial performance of the polylactic acid film can be remarkably improved; in addition, kudzuvine root polysaccharide and konjac glucomannan form a slow-release layer on the surface of the film, antibacterial components are gradually released through hydrogen bonds, the antibacterial performance of the film can be kept for a long time, and the problem that a traditional antibacterial agent is prone to migration and failure is solved.

The invention discloses a nano sound-sensitive agent for treating and preventing chronic osteomyelitis and a preparation method thereof, and the preparation method comprises the following steps: step 1, dropwise adding a zinc acetate solution into a dimethylimidazole solution, fully reacting, and centrifugally washing to obtain ZIF-8; step 2, adding a manganese-containing compound into the ZIF-8 solution, fully reacting, and centrifugally washing to obtain Mn < 2 + >-doped ZIF-8 nanoparticles; and step 3, adding the Mn < 2 + >-doped ZIF-8 nanoparticles into a trehalose solution, fully stirring and mixing, and centrifugally washing to obtain the required sound-sensitive agent. The sonosensitizer obtained by the invention can gradually degrade and release Mn < 2 + > in an acidic microenvironment of an osteomyelitis infected part. The released Mn < 2 + > further enhances the sensitivity of cGAS to bacterial DNA, effectively activates an STING pathway in DCs, promotes maturation of the DCs, enhances the antigen presentation capability, and then promotes proliferation and differentiation of T cells and induces generation of memory B cells, thereby being beneficial to prevention of hidden survival and infection recurrence of bacteria.

The application belongs to the technical field of DNA extraction, and particularly relates to a field rapid extraction method of DNA in plant bacterial disease lesion, which comprises the following steps: mixing plant disease leaf lesion tissue and a lysis solution, and heating in a water bath in a thermos cup to complete nucleic acid release; the lysis solution comprises 0.05-0.2 mol / L NaOH; preferably, the lysis solution comprises 0.05-0.2 mol / L NaOH, 0.01% SDS and 0.1 mmol / L EDTA. The DNA extraction method established by the application does not need precise instruments and toxic reagents, is simple to operate, short in time consumption, and can realize field rapid extraction of plant lesion bacterial DNA, thereby providing technical support for early diagnosis and accurate prevention and control of plant bacterial diseases in the field.

The invention discloses a compound nucleic acid immunological enhancement substance with efficient delivery capacity as well as a preparation method and application thereof, the compound nucleic acid immunological enhancement substance comprises a VP1-N2 aa 23-43 polypeptide derived from chicken infectious anemia virus and an exogenous active molecule, and the amino acid sequence of the VP1-N2 aa 23-43 polypeptide is shown as SEQ ID NO. 1. The efficient cell-penetrating peptide VP1-N2 aa 23-43 polypeptide is designed, the polypeptide can carry CpG to penetrate into different cells within 30 min, an immune system is activated by simulating a CpG motif in bacterial DNA, a TLR9 signal channel is activated, and immune response is enhanced. Therefore, the VP1-aa 23-43 polypeptide compound nucleic acid immunological enhancement substance with high-efficiency delivery capacity is used for enhancing the immune response of the body to the vaccine antigen and improving the protection effect of the vaccine.

The invention relates to the technical field of biology, in particular to application of an antibacterial peptide with antibacterial activity on Xanthomonas oryzae pv. Oryzae, the antibacterial peptide contains 11 amino acids, the C terminal of the antibacterial peptide is amidated, and the amino acid sequence of the antibacterial peptide is Trp-Lys-Leu-Phe-Lys-Lys-Ile-Leu-Lys-Val-Leu-NH2. The antibacterial peptide is of a typical amphiphilic alpha-helical structure. According to the present invention, the antibacterial peptide has significant antibacterial activity on Xanthomonas oryzae pv.oryzae, Xoo, the minimum inhibitory concentration of the antibacterial peptide is 1.5625 [mu] g / mL, the minimum bactericidal concentration of the antibacterial peptide is 3.125 [mu] g / mL, and the half maximum inhibitory concentration of the antibacterial peptide is 1.14 [mu] g / mL; the antibacterial peptide can cause membrane permeability increase, cause membrane structure damage and cause calcium ion leakage. Moreover, the compound can effectively penetrate through pathogenic bacteria cell membranes to trigger excessive accumulation of active oxygen in pathogenic bacteria cells, so that oxidative damage is caused. The antibacterial peptide can also be combined with bacterial DNA to interfere the replication and repair process of genetic materials. On rice leaves, the antibacterial peptide can significantly inhibit disease development.

The subject invention provides fluorophore-quencher nucleic acid molecules comprising relaxed or supercoiled DNA molecules, and their use in rapid and efficient high-throughput screening (HTS) assays to screen and identify compounds that inhibit DNA gyrases. These compounds can be used as antibiotics for treating bacterial infections, especially, multidrug resistant bacterial infections.

We disclose a biochemical compound comprising CpG moieties derived from human commensal bacteria that exhibit the ability to suppress immune system responses in patients that suffer from autoimmune and allergic diseases arising from overactive immune systems. We outline several methods for use of the compound in the treatment of individuals suffering from various types of auto-immune and related diseases.

The invention relates to a probe for predicting, diagnosing or monitoring bacterial infection, a kit and application thereof, probe sequences (SAP, ECP1 and ECP2) specifically bind to bacterial DNA (such as Staphylococcus aureus and Escherichia coli), characteristic electrochemical signals are generated through a solid nanopore platform, the probe can recognize specific bacterial DNA sequences, and the specific bacterial DNA sequences can be used for predicting, diagnosing or monitoring bacterial infection. And through the structural design of the probe, multiple bacteria can be detected at the same time. The solid nanopore technology adopted by the invention can complete extraction and detection of bacterial DNA in a sample within about 1 hour, the detection limit reaches 1 * 10 < 3 > CFU / mL, and the detection period is remarkably shortened. Compared with a traditional bacterial culture method, the method has high detection sensitivity and specificity on the premise of not depending on amplification, and is suitable for early screening and rapid diagnosis of clinical infection.

Some embodiments relate to techniques for enriching eukaryotic DNA in a DNA sample containing eukaryotic DNA and prokaryotic DNA. A DNA sample comprising eukaryotic DNA and prokaryotic DNA can be accessed. The DNA sample can be treated with one or more restriction enzymes, where at least one of the one or more restriction enzymes is a methylation-specific restriction enzyme that selectively digests prokaryotic DNA by targeting methylation patterns characteristic of prokaryotic genomes, the methylation patterns being absent or rare in eukaryotic DNA. The treated DNA sample can be sequenced.

The invention relates to the technical field of pet treatment, and discloses an atomization treatment box for pets, the atomization treatment box comprises an atomization box, an atomization treatment mechanism and a cleaning and disinfection mechanism are fixedly mounted outside the atomization box, and an equipment box is fixedly mounted at the bottom of the atomization box; and a cleaning water tank, a disinfection water tank and a conveying pump are fixedly mounted in the equipment box. According to the atomization treatment box for the pets and the using method, after treatment is finished, clear water in the cleaning water tank is driven by the conveying pump to conduct high-pressure flushing on the inner wall of the atomization box and a bearing net through a nozzle of the spraying pipe, and excrement residues are removed; then, an electric three-way valve is switched to introduce disinfectant in a disinfection water tank for spray disinfection, and finally, an ultraviolet disinfection lamp is turned on to emit ultraviolet rays with specific wavelengths to penetrate through bacteria DNA to destroy the replication ability of the bacteria, so that physical sterilization is realized; according to the process, through triple treatment of automatic cleaning, disinfection and ultraviolet rays, it is guaranteed that no residual pathogen exists in the box body, and the cross infection risk is reduced.

The present invention relates to a biosensor, a method for obtaining same and the use thereof to detect Pseudomonas aeruginosa in clinical samples from patients, based on the use of molecular gates on porous organic–inorganic hybrid supports that allow specific and selective recognition of bacterial DNA. The molecular gate developed comprises an oligonucleotide sequence complementary to a specific region of the DNA of this bacterium. The external surface of the porous support is chemically modified to provide reactive groups capable of bonding with short-sequence oligonucleotides (O1) designed to act as an anchor and to subsequently bond oligonucleotides (O2) specific for the recognition of P. aeruginosa. In the presence of P. aeruginosa genomic DNA, the oligonucleotides (O2) are capable of specific recognition by complementarity and of detaching from the pore surface, leading to the release of a fluorescent indicator.