16 results about "Bacterial dna" patented technology

This invention provides a detection method for distinguishing between live and dead bacteria of the watermelon fruit spot bacterial disease fungus. [Solution] A detection method for distinguishing between live and dead bacteria based on PMA-PCR technology, comprising: a step of collecting and suspending bacteria in a sample to be detected, which involves suspending the sample in physiological saline to obtain a bacterial suspension and heating it in a 100°C water bath for 10 minutes to prepare a dead bacterial suspension; a step of PMA treatment, which involves adding propidium monoazide (PMA) to the bacterial suspension; a step of blue light crosslinking, which involves irradiating the PMA-treated bacterial suspension with blue light to activate the PMA and covalently bind it to the dead bacterial DNA; a step of DNA extraction, which involves collecting bacterial cells by centrifugation and extracting DNA using a kit method; a PCR amplification step, which involves amplification using a specific primer pair; and a step of detecting the amplified product by agarose gel electrophoresis, which involves determining the result, which involves a step of detecting the product by agarose gel electrophoresis, wherein a target band appears in the live bacterial sample and no band appears in the dead bacterial sample.

This application relates to a method, apparatus, computer device, computer-readable storage medium, and computer program product for bacterial phenotypic prediction based on bacterial DNA, which can be used in the field of bioinformatics. The method includes: constructing a heterogeneous graph; the heterogeneous graph includes nodes and the connections between nodes; performing semantic training processing on mutation embedding information based on the heterogeneous graph to obtain mutation semantic embedding information of the mutation embedding information; determining the target mutation semantic embedding information corresponding to the mutation information in the input sample based on the mutation semantic embedding information; performing aggregation processing on the target mutation semantic embedding information to obtain aggregated feature information of the target mutation semantic embedding information; inputting the aggregated feature information into a preset drug resistance prediction model for drug resistance prediction processing to obtain the drug resistance prediction result of the input sample against anti-tuberculosis drugs. This method can improve the efficiency of drug resistance prediction.

The invention discloses a sampling chip and a sampling method for DNA of bacteria. The chip is composed of a bottom plate, a middle plate and an upper plate which are integrated through thermocompression bonding, a reaction cavity, a liquid storage cavity, a waste liquid cavity and microfluidic channels which are communicated with one another are sequentially arranged on the bottom plate, paraffin valves capable of being controlled by heating are arranged in the channels, and heating pieces are arranged at the bottom of the reaction cavity. According to the chip, a gas source is introduced through a gas inlet, and the steps of sample loading, cell lysis, supernate removal, ethanol cleaning, pneumatic drying, DNA elution and the like are realized in a closed flow path. The method comprises the following steps: loading and sealing a bacterium-containing sample, heating, splitting and combining, discharging supernatant under the driving of a gas source, introducing a cleaning solution, heating and volatilizing residual liquid, injecting an eluent, desorbing DNA at a constant temperature, and finally collecting the eluent. According to the scheme, extraction of bacterial DNA is completed in a single chip, dependence on external equipment such as centrifugation and pipetting is reduced, and a DNA sample which can be directly used for downstream amplification or sequencing analysis is obtained.

The application discloses a bacteria with limited reproduction capacity, a method for controlling bacterial reproduction and application, and belongs to the technical field of biotechnology and cell immunotherapy. The bacterial DNA comprises an unnatural amino acid orthogonal translation module, a suicide module and a rescue module. Based on the unnatural amino acid (ncAA) orthogonal translation system, the orthogonal genetic barrier theory is used, gene circuit design and element modularization are used, bacterial reproduction generations are accurately controlled, bacterial growth is effectively controlled, and the bacteria are prevented from escaping, and the bacteria have wide applicability. The bacteria and the method have wide application prospects in the development of attenuated live vaccines.

The invention discloses a thermophilic thermophilic bacteria fermentation product, a preparation method and application thereof and an oil control product, and belongs to the technical field of cosmetics. Thermophilic thermophilic bacteria are cultured in a first-stage seed solution culture medium and a second-stage seed solution culture medium in sequence, the thermophilic thermophilic bacteria are promoted to the logarithmic phase, then a mulberry leaf extracting solution is added for fermentation, and the oil control product is obtained. According to the method, the growth stability of thalli is effectively prolonged, the stability of a fermentation microenvironment is maintained, the metabolic activity of the thermophilic thermophilic bacteria is improved, normal replication of thallus DNA at high temperature can be guaranteed, and the thermophilic thermophilic bacteria can grow at a high speed in the whole growth period. According to the method disclosed by the invention, the accumulation of byproducts can be avoided, active ingredients with an oil control effect in the thermophilic thermophilic bacteria are effectively enriched, and a thermophilic thermophilic bacteria fermentation product which has the effects of improving the mRNA expression quantity of AMPK, effectively inhibiting the activity of 5 alpha-reductase, inhibiting sebaceous gland cells from synthesizing lipid droplets and reducing hormone-induced lipid synthesis is obtained; the oil control activity is excellent.

Methods are provided for detecting disease in liquid biopsy samples by identifying microbial rRNA. The methods include isolating nucleic acids from extracellular vesicles, treating the nucleic acids with DNase to remove DNA including human cfDNA, mcfDNA, and contaminating microbial DNA, and then amplifying a region of a microbial phylogenetic rRNA. To further reduce contaminating DNA, the amplifying step can include reverse transcription to translate the phylogenetic region into cDNA and extend the cDNA ends with a T7 promoter and sequencing adaptors. The translated region is amplified with primers to the extended ends of the cDNA and sequenced. The resulting phylogenetic microbial sequences are useful for diagnosis and monitoring of diseases including cancer. The method can further include amplification of other human or bacterial DNA or RNA sequences to augment disease diagnosis. The method results in a very low contribution of contaminating microbial DNA and RNA to the overall microbial signature.

The application discloses a bacterial fluorescence detection method based on asymmetric RPA and hydrogel. The bacterial detection sample, asymmetric RPA reagent, molecular beacon, lysozyme and hydrogel monomer are jointly added into a chip groove to form a hydrogel; the lysozyme first cracks the bacteria and releases DNA, and then the RPA is used for asymmetric amplification to generate a single-stranded amplicon; the single-stranded amplicon is hybridized with the molecular beacon to restore fluorescence, and the hydrogel network restricts the diffusion of bacterial DNA, single-stranded amplicon and hybridization chain, so that the fluorescence hybridization chain forms an aggregation point near the bacteria; the aggregation points are counted by a fluorescence microscope to obtain the number of target bacteria. The application cracks the bacteria and performs asymmetric isothermal amplification in the hydrogel in situ, shortens the detection time, and does not need long bacterial culture and DNA purification steps and complex instruments or professional technical operation. The system is stable, easy to carry, and suitable for rapid detection in a resource-limited scene.

This invention relates to the field of biomedical technology, specifically disclosing a biomimetic bacterial DNA nanomicelle, its preparation method, and its applications. By mimicking the structural and functional characteristics formed when microorganisms infect a host, exogenous nucleic acid stimulation signals and oxidative stress regulation functions are integrated into a single nanostructure. A micellar nanostructure is designed, comprising a complex of oligonucleotides containing CpG motifs and ferrocene groups, which is formed through self-assembly in solution. This nanomicelle can significantly promote the proliferation or differentiation of dendritic cells, improve their maturity, and enhance their immune activation capacity; by enhancing the cross-presentation capacity of dendritic cells to target antigens, it effectively promotes cytotoxic CD8+. + T-cell immune responses; it can also enhance the body's cellular immune response by inducing the activation and proliferation of antigen-specific cells. These DNA nanomicelles have significant clinical application value in areas such as vaccine adjuvants, tumor immunotherapy, and immune regulation.

The subject invention provides fluorophore-quencher nucleic acid molecules comprising relaxed or supercoiled DNA molecules, and their use in rapid and efficient high-throughput screening (HTS) assays to screen and identify compounds that inhibit DNA gyrases. These compounds can be used as antibiotics for treating bacterial infections, especially, multidrug resistant bacterial infections.

The application belongs to the technical field of DNA extraction, and particularly relates to a field rapid extraction method of DNA in plant bacterial disease lesion, which comprises the following steps: mixing plant disease leaf lesion tissue and a lysis solution, and heating in a water bath in a thermos cup to complete nucleic acid release; the lysis solution comprises 0.05-0.2 mol / L NaOH; preferably, the lysis solution comprises 0.05-0.2 mol / L NaOH, 0.01% SDS and 0.1 mmol / L EDTA. The DNA extraction method established by the application does not need precise instruments and toxic reagents, is simple to operate, short in time consumption, and can realize field rapid extraction of plant lesion bacterial DNA, thereby providing technical support for early diagnosis and accurate prevention and control of plant bacterial diseases in the field.

The invention discloses a compound nucleic acid immunological enhancement substance with efficient delivery capacity as well as a preparation method and application thereof, the compound nucleic acid immunological enhancement substance comprises a VP1-N2 aa 23-43 polypeptide derived from chicken infectious anemia virus and an exogenous active molecule, and the amino acid sequence of the VP1-N2 aa 23-43 polypeptide is shown as SEQ ID NO. 1. The efficient cell-penetrating peptide VP1-N2 aa 23-43 polypeptide is designed, the polypeptide can carry CpG to penetrate into different cells within 30 min, an immune system is activated by simulating a CpG motif in bacterial DNA, a TLR9 signal channel is activated, and immune response is enhanced. Therefore, the VP1-aa 23-43 polypeptide compound nucleic acid immunological enhancement substance with high-efficiency delivery capacity is used for enhancing the immune response of the body to the vaccine antigen and improving the protection effect of the vaccine.

The subject invention provides fluorophore-quencher nucleic acid molecules comprising relaxed or supercoiled DNA molecules, and their use in rapid and efficient high-throughput screening (HTS) assays to screen and identify compounds that inhibit DNA gyrases. These compounds can be used as antibiotics for treating bacterial infections, especially, multidrug resistant bacterial infections.

We disclose a biochemical compound comprising CpG moieties derived from human commensal bacteria that exhibit the ability to suppress immune system responses in patients that suffer from autoimmune and allergic diseases arising from overactive immune systems. We outline several methods for use of the compound in the treatment of individuals suffering from various types of auto-immune and related diseases.

The invention relates to a probe for predicting, diagnosing or monitoring bacterial infection, a kit and application thereof, probe sequences (SAP, ECP1 and ECP2) specifically bind to bacterial DNA (such as Staphylococcus aureus and Escherichia coli), characteristic electrochemical signals are generated through a solid nanopore platform, the probe can recognize specific bacterial DNA sequences, and the specific bacterial DNA sequences can be used for predicting, diagnosing or monitoring bacterial infection. And through the structural design of the probe, multiple bacteria can be detected at the same time. The solid nanopore technology adopted by the invention can complete extraction and detection of bacterial DNA in a sample within about 1 hour, the detection limit reaches 1 * 10 < 3 > CFU / mL, and the detection period is remarkably shortened. Compared with a traditional bacterial culture method, the method has high detection sensitivity and specificity on the premise of not depending on amplification, and is suitable for early screening and rapid diagnosis of clinical infection.

Some embodiments relate to techniques for enriching eukaryotic DNA in a DNA sample containing eukaryotic DNA and prokaryotic DNA. A DNA sample comprising eukaryotic DNA and prokaryotic DNA can be accessed. The DNA sample can be treated with one or more restriction enzymes, where at least one of the one or more restriction enzymes is a methylation-specific restriction enzyme that selectively digests prokaryotic DNA by targeting methylation patterns characteristic of prokaryotic genomes, the methylation patterns being absent or rare in eukaryotic DNA. The treated DNA sample can be sequenced.

The present invention relates to a biosensor, a method for obtaining same and the use thereof to detect Pseudomonas aeruginosa in clinical samples from patients, based on the use of molecular gates on porous organic–inorganic hybrid supports that allow specific and selective recognition of bacterial DNA. The molecular gate developed comprises an oligonucleotide sequence complementary to a specific region of the DNA of this bacterium. The external surface of the porous support is chemically modified to provide reactive groups capable of bonding with short-sequence oligonucleotides (O1) designed to act as an anchor and to subsequently bond oligonucleotides (O2) specific for the recognition of P. aeruginosa. In the presence of P. aeruginosa genomic DNA, the oligonucleotides (O2) are capable of specific recognition by complementarity and of detaching from the pore surface, leading to the release of a fluorescent indicator.