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54results about How to "Save testing cost" patented technology

Detection device for monitoring water quality on line and water quality on-line monitoring method

The invention provides a detection device for monitoring water quality on line. The detection device comprises a sampling device, a microorganism fixing device, and an electrochemical detection device wherein a sample inlet of the microorganism fixing device is connected with a sample outlet of the sampling device; the microorganism fixing device is a microbial film reactor; the microbial film reactor comprises a container and a microbial film arranged in the container; the microbial film comprises a microbial carrier and a microorganism attached to the microbial carrier; the microbial film reactor is placed in a thermostat; a sample inlet of the electrochemical detection device is connected with a sample outlet of the microbial film reactor; the electrochemical detection device comprises a detection pool, an electrode system, an electrochemical work station and a program control device; the electrode system is arranged in the detection pool; the electrochemical work station is connected with the electrode system; and the program control device is used for controlling the electrochemical work station. The invention further provides a water quality on-line monitoring method. When the on-line monitoring device detects the biochemical oxygen demand and the toxicity of the water sample, the result is accurate.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI

Method for simultaneously detecting twelve kinds of common respiratory viruses

The invention discloses a method for simultaneously detecting twelve kinds of common respiratory viruses. According to the method, primers and probes are designed according to gene conservative areas of the twelve kinds of common respiratory viruses, namely influenza A virus, influenza B virus, influenza C virus, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, rhinovirus, Bocavirus, adenovirus, coronavirus, metapneumovirus and respiratory syncytial virus, nucleic acid fragments of samples to be measured are extracted for amplifying, and finally, the samples are separated by using a capillary electrophoresis method. The method disclosed by the invention has the advantages of low required sample size, high sensitivity and accuracy, good specificity and low cost; the defects that the conventional single tube multiplex fluorescence PCR (Polymerase Chain Reaction) detection primers are difficult to design, and multicolor fluorescence mutually intervenes and is not easy to part are overcome, the defects that a chip detection method is tedious in operation, high in detection cost and the like are also overcome, and a new method is provided for screening the respiratory viruses.
Owner:FUJIAN INT TRAVEL HEALTH CARE CENT +1

Manufacturing method of back drill holes on PCB (Printed Circuit Board)

The invention discloses a manufacturing method of back drill holes on a PCB (Printed Circuit Board). The manufacturing method comprises the following steps of: in a manufacturing process of the PCB, dividing all layers of the PCB into layers for forming a metalized hole layer and layers for forming a non-metalized hole layer according to layered distribution of metalized holes and non-metalized holes of the back drill holes on the PCB to be manufactured; respectively manufacturing and processing all the layers forming the metalized hole layer and all the layers forming the non-metalized hole layer to form a metalized hole layer with the metalized holes and a non-metalized layer with the non-metalized holes; and laminating the metalized hole layer with the metalized holes and the non-metalized layer with the non-metalized holes through a prepreg with low fluidity to form the PCB with the back drill holes. Therefore, according to the manufacturing method disclosed by the invention, useless hole copper can be completely eliminated, so that the back drill holes without useless hole copper residual can be manufactured on the PCB, and the signal transmission integrity of the PCB is ensured so as to adapt to development requirements of electronic products.
Owner:CHINA CIRCUIT TECH SHANTOU CORP +1

Kit for synchronously detecting twenty-three meningitis pathogens and detection method of kit

The invention discloses a kit for synchronously detecting twenty-three meningitis pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the twelve meningitis pathogens and a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-13 (sequence identifier number 1-13), and the PCR primer comprises forward and reverse PCR amplification primers of the rest eleven meningitis pathogens, a human DNA internal reference and a reaction internal reference, and PCR amplification primers of the twelve meningitis pathogens and the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 14-52. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

Primer composition for guiding nitroglycerin medication and healthy drinking, multiple gene detection kit and use method of kit

The invention discloses a primer composition for guiding nitroglycerin medication and healthy drinking, a multiple gene detection kit and a use method of the kit, and the kit comprises the primer composition, a PCR buffer solution and a positive reference substance, and the PCR buffer solution comprises ultrapure water, an X solution, a 10*PCR (polymerase chain reaction) buffer solution, a PCR primer, a 25mM magnesium chloride solution and DNA polymerase, the primer composition comprise two forward and reverse amplification primers of different gene types on the 2 SNP sites of genes related to the nitroglycerin medication and healthy drinking and forward and reverse amplification primers capable of reflecting internal reference, the gene sequences of the primers are represented as SEQ ID NO.1-NO.8; the use method comprises the step of acquiring a sample and extracting nucleic acid, the step of performing the PCR reaction by using extracted nucleic acid as a template, and the final step of separating the sample through capillary electrophoresis. The primer composition has the advantages of being strong in specificity, high in accuracy, high in flux, strong in reliability, low in cost and free from false negative result.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

Bridge expansion joint displacement monitoring device

InactiveCN106840064AAccurate and timely detection of deformation failure problemsDiscover deformation failure problemsMeasurement devicesElectricityEngineering
The invention discloses a bridge expansion joint displacement monitoring device. There is a first beam plate on one side of the bridge expansion joint and a second beam plate on the other side, including a horizontal spring body, a microprocessor, an early warning device, and a first tension pressure sensor. , the second tensile pressure sensor, fit the first shaped steel fixed on the end face of the first beam plate, fit the second shaped steel fixed on the end face of the second beam plate, the first shaped steel is provided with a first notch groove, and the second shaped steel The section steel is provided with a second notch groove, the first tension pressure sensor is embedded in the first notch groove, the second tension pressure sensor is embedded in the second notch groove, and the first tension pressure sensor is connected to the second tension sensor through the spring body. The tension and pressure sensors are connected, and both the first tension and pressure sensors and the second tension and pressure sensors are electrically connected to the early warning device through the microprocessor. The invention realizes accurate detection of the displacement of expansion joints parallel to and perpendicular to the axis of the bridge, has high efficiency, strong real-time performance, safe and simple inspection work, reliable and comprehensive results, and greatly saves detection costs and detection procedures.
Owner:CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Kit for synchronously detecting fifteen hemorrhagic fever pathogens and detection method of kit

ActiveCN103074452AMonitor reaction efficiencyEnsuring Quality JudgmentsMicrobiological testing/measurementMicroorganism based processesPositive controlReverse transcriptase
The invention discloses a kit for synchronously detecting fifteen hemorrhagic fever pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the nine hemorrhagic fever pathogens and a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-10 (sequence identifier number 1-10), and the PCR primer comprises forward and reverse PCR amplification primers of the rest six hemorrhagic fever pathogens, a human DNA internal reference and a reaction internal reference, and PCR amplification primers of the nine hemorrhagic fever pathogens and the human RNA internal reference, and has a gene sequence show as SEQ ID NO. 10-36. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

PCB manufacturing method for conveniently detecting back drilling hole precision

The invention relates to the field of printed circuit board manufacturing and specifically relates to a PCB manufacturing method for conveniently detecting back drilling hole precision. According to the method, an open loop conductive annular line is arranged around each back drilling position; after back drilling operation is completed, a universal meter is used for detecting whether the annular line is open or not via detection points; if the annular line is open, the annular line is broken via drilling operation; if the annular line is short circuited, the annular line is not broken via the drilling operation. Via measurement, identification marks of short circuits are written down, and the identification marks indicate numerical values of distance between back drilling holes and annular lines and numbers of layers on which the annular lines are positioned; via common add-and-subtract operation, accurate offset can be known in a visual manner, and back drilling hole precision can be read in a simple and convenient manner. When the back drilling holes are large in quantity, poor quality holes can be identified easily. Via use of the method, simple operation can be achieved, detection cost and man power cost can be greatly lowered, and working efficiency can be improved.
Owner:SHENZHEN SUNTAK MULTILAYER PCB

Turbot fluorescence labeling microsatellite sextuple PCR family tree recognizing method

The invention relates to a turbot fluorescence labeling microsatellite sextuple PCR family tree recognizing method which comprises the following steps: using three kinds of fluorescence (6FAM, HEX andNED) for pairwise labeling six pairs of primers according to the sizes of the PCR amplifying products of the primers; using primer design software for excluding the possibility that the six pairs ofprimers form dipolymer in the PCR reaction process, and combining the six pairs of primers in the same PCR reaction system which comprises ROX fluorescence molecule internal labels; adjusting the PCRreaction system, and establishing parameters which can enable the six pairs of primers to be simultaneously amplified in the same PCR reaction system and a proper PCR reaction procedure; and analyzingPCR products in six microsatellite positions of the turbot through ABI3130 or a similar gene analyzer according to the fluorescence labels. The sextuple PCR technology can improve the detection efficiency of the turbot microsatellite labeling, wherein the single parent excluding ratio is 99.63%, and the individual recognizing ratio is 99.99%; and the sextuple PCR technology can distinguish different family trees of the turbot or recognize and identify individuals and parent children.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Method for detecting animal pathogenic microorganisms and special protein chip thereof

The invention belongs to the field of biomedicine, providing a method for detecting animal pathogenic microorganisms and a special protein chip thereof. The detecting method essentially comprises the following steps of: a. preparing the protein chip, selecting a plurality of different groups of specific antigen of many kinds of pathogenic microorganisms by means of an experiment to be fixed on a properly modified carrier; and b. detecting: reacting blood serum of a animal to be detected with the protein chip, hybridizing with a marked secondary antibody, detecting a hybrid positive signal in a proper detection way, and obtaining a hybrid result of the blood serum and the chip by means of scanning and analyzing, wherein the blood serum infected with the different pathogenic microorganisms can show different positive signal combination modes after the reaction, so as to ensure whether the animal to be detected is infecting or is infected with the pathogenic microorganisms marked on the protein chip. The invention can simultaneously detect many kinds of the pathogenic microorganisms, improves detecting efficiency, selects many groups of different antigen with a certain specificity aiming at each pathogenic microorganism, leads the detection to have good specificity, and has good application prospect in detecting the animal pathogenic microorganisms.
Owner:INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI

Overall type steel-based platform static load test load piling method for civil engineering

The invention relates to an overall type steel-based platform static load test load piling method for civil engineering. The overall type steel-based platform static load test load piling method for civil engineering is characterized in that the method is performed according to the following steps that, a steel-based platform is selected, a jack is installed at the center of test points, a main beam is installed on the steel-based platform, a jack cylinder body and the main beam are effectively connected, and the steel-based platform and the main beam are effectively connected; a reference beam is installed, and loads are piled; after the weight of the piled loads reaches a regulated weight and a test instrument meets the test requirement according to check, test loading can be performed;after a test ends, parts of the loads are unloaded; and the steel-based platform, the main beam and part of the loads are transferred to a next test point, the above steps are repeated till detectionof the test points of the whole program ends. By means of the load piling method, the large tonnage load piling potential safety hazards can be eliminated, the detection time and the detection cost are saved, a large tonnage static load experiment can be safely and conveniently implemented, integrity is good, self moving can be achieved, speed is high, cost is low, and energy conservation and environmental protection are achieved.
Owner:张宗毅

Chemical indicator monitoring disinfection effect of oxidized disinfectant and monitoring method

InactiveCN109668884AFast monitoring resultsColor indication changes significantlyMaterial analysis by observing effect on chemical indicatorDisinfectantOxygen
The invention discloses a chemical indicator monitoring the disinfection effect of an oxidized disinfectant. The chemical indicator is prepared from an indicating compound and an ink composition. Theindicating compound is triarylmethane pigment, and the mass percentage of the indicating compound to the ink composition is (6-10%) to (90-94%). The color of the triarylmethane pigment can change obviously when being exposed an oxidized disinfectant disinfection process. The method for monitoring the oxidized disinfectant disinfection process comprises the following steps that the chemical indicator and a to-be-disinfected object are placed under a same disinfection condition; color changing of the chemical indicator is observed to judge a disinfection effect, when the chemical indicator is exposed to a wet oxidized disinfectant, especially ozone, the color of the chemical indicator changes obviously; and when the chemical indicator is exposed to a dry oxidized disinfectant or oxygen, thecolor does not change. The chemical indicator provided by the invention can rapidly monitor the disinfection result, has the obvious color indicating change, and has the relatively good indicating effect.
Owner:HENAN FANGZHOU MEDICAL INSTR CO LTD

Method for determining dissolution rate of medicinal preparation containing acetaminophen, dextromethorphan hydrobromide and doxylamine succinate

The invention discloses a method for determining a dissolution rate of a medicinal preparation containing acetaminophen, dextromethorphan hydrobromide and doxylamine succinate. The method comprises the following steps of: (1) adding medicinal preparation containing acetaminophen, dextromethorphan hydrobromide and doxylamine succinate into a dissolution medium for dissolution treatment, performing sampling and filtering to obtain dissolution liquid; (2) detecting the contents of acetaminophen, dextromethorphan hydrobromide and doxylamine succinate in the dissolution liquid; and (3) and determining the dissolution rate of the medicinal preparation according to the detection result of the step (2). The method can effectively simulate the dissolution behavior in vivo in vitro, can determine the dissolution rate of the soft capsule containing acetaminophen, dextromethorphan hydrobromide and doxylamine succinate, and provides technical support for the research and development and quality control of medicaments. The method can simultaneously determine the dissolution results of the three substances under the same condition, greatly saves the detection time and the detection cost, and has good accuracy and reproducibility.
Owner:安士制药(中山)有限公司

Preparation method of sodium hypochlorite

The invention discloses a preparation method of sodium hypochlorite. According to the invention, a sodium hydroxide solution is utilized to absorb chlorine gas to prepare the sodium hypochlorite. The preparation method is implemented in a way that: any time before the preparation is finished, measuring the reaction tank liquid level M1 and the free alkali content N1 at that time, and determining the finishing reaction tank liquid level Mend according to the formula Mend=M1+(N1-Nend); and monitoring the reaction tank liquid level M in the subsequent reaction process, and finishing the reaction when M=Mend. According to the relationship between free alkali content difference and reaction liquid value difference of the sodium hypochlorite solution at different liquid levels in the production process, a qualified edge point of the sodium hypochlorite solution is determined by a liquid level metering method, and an empirical formula is educed; and the reaction tank liquid level is monitored to accurately judge the qualified reaction end point of the sodium hypochlorite solution, thereby effectively and conveniently controlling the production qualification rate of the sodium hypochlorite solution, greatly lowering the labor intensity of personnel, reducing the sampling frequency, relieving the problem of environmental pollution on the spot due to sampling, and avoiding the phenomenon of unqualified technical indexes due to shortness of hands or untimely inspection.
Owner:RUYUAN DONGYANGGUANG ELECTROCHEM FACTORY
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