Double-fluorescent PCR detection primer, probe, reaction liquid and kit capable of detecting pathogens of respiratory tract

A detection kit and dual fluorescence technology, which is applied in the field of dual fluorescence PCR detection primers, probes, reaction solutions and kits, can solve the problems of a large number of operation steps for pathogens, time-consuming and laborious, etc., and achieve a wide range of applications, simple operation and practicality strong effect
CN105463129AInactive Publication Date: 2016-04-06SHENZHEN INT TRAVEL HEALTHCARE CENT +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENZHEN INT TRAVEL HEALTHCARE CENT
Publication Date
2016-04-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a double-fluorescent PCR detection primer, a probe, reaction liquid and a kit capable of rapidly detecting 16 pathogens of the respiratory tract. The kit comprises pre-subpackaged PCR reaction liquid, RT-PCR enzyme, a positive quality control product and a negative quality control product, wherein the pre-subpackaged PCR reaction liquid is provided with a primer and a TaqMan probe for detecting at least two of the following pathogens: respiratory syncytial virus, enterovirus, coronavirus NL63, coronavirus HKU1, coronavirus 229E, coronavirus OC43, parainfluenza virus type I, parainfluenza virus type II, rhinovirus, parainfluenza virus type III, human bocavirus, human metapneumovirus, mycoplasma pneumoniae, chlamydia pneumoniae, adenovirus and legionella pneumophila. The kit is convenient to operate, and can be used for at most screening 16 syndrome pathogens of the respiratory tract within 2 hours, so that the detection time and cost are greatly saved. The kit has the greatest advantages of simplicity in operation and strong practicability, and can be easily popularized in laboratories of the ports.
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Description

technical field

[0001] The invention relates to a double fluorescent PCR (polymerase chain reaction) detection primer, a probe, a reaction solution and a kit capable of rapidly detecting 16 respiratory pathogens, and belongs to the technical field of nucleic acid detection. Background technique

[0002] Fever Respiratory Syndrome (FRS) is one of the most important group of infectious diseases in my country at this stage. Through the implementation of the major scientific and technological projects of 'Prevention and Treatment of Major Infectious Diseases such as AIDS and Viral Hepatitis' during the Eleventh Five-Year Plan and the Twelfth Five-Year Plan, my country has established a nationwide The interdepartmental and interdisciplinary infectious disease prevention and control support technology platform has realized the improvement of the emergency response capability of infectious diseases from point to point. my country's febrile respiratory syndrome monitoring program (20...

Claims

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