Double-fluorescent PCR detection primer, probe, reaction liquid and kit capable of detecting pathogens of respiratory tract

A detection kit and dual fluorescence technology, which is applied in the field of dual fluorescence PCR detection primers, probes, reaction solutions and kits, can solve the problems of a large number of operation steps for pathogens, time-consuming and laborious, etc., and achieve a wide range of applications, simple operation and practicality strong effect

Inactive Publication Date: 2016-04-06
SHENZHEN INT TRAVEL HEALTHCARE CENT +1
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Problems solved by technology

[0003] Respiratory diseases can be caused by dozens of pathogens, and the final confirmation of pathogens requires a large number of operational steps, which is time-consuming and labor-intensive

Method used

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  • Double-fluorescent PCR detection primer, probe, reaction liquid and kit capable of detecting pathogens of respiratory tract
  • Double-fluorescent PCR detection primer, probe, reaction liquid and kit capable of detecting pathogens of respiratory tract
  • Double-fluorescent PCR detection primer, probe, reaction liquid and kit capable of detecting pathogens of respiratory tract

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Embodiment Construction

[0039] The present invention will be further described below in conjunction with specific examples, and the advantages and characteristics of the present invention will become clearer along with the description. However, these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0040] In order to quickly and accurately complete the screening of respiratory syndrome pathogens and save costs, the following embodiments of the present invention intend to develop a dual fluorescent PCR detection kit that can quickly detect 16 respiratory pathogens at the same time. This method mainly relies on the method of ...

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Abstract

The invention discloses a double-fluorescent PCR detection primer, a probe, reaction liquid and a kit capable of rapidly detecting 16 pathogens of the respiratory tract. The kit comprises pre-subpackaged PCR reaction liquid, RT-PCR enzyme, a positive quality control product and a negative quality control product, wherein the pre-subpackaged PCR reaction liquid is provided with a primer and a TaqMan probe for detecting at least two of the following pathogens: respiratory syncytial virus, enterovirus, coronavirus NL63, coronavirus HKU1, coronavirus 229E, coronavirus OC43, parainfluenza virus type I, parainfluenza virus type II, rhinovirus, parainfluenza virus type III, human bocavirus, human metapneumovirus, mycoplasma pneumoniae, chlamydia pneumoniae, adenovirus and legionella pneumophila. The kit is convenient to operate, and can be used for at most screening 16 syndrome pathogens of the respiratory tract within 2 hours, so that the detection time and cost are greatly saved. The kit has the greatest advantages of simplicity in operation and strong practicability, and can be easily popularized in laboratories of the ports.

Description

technical field [0001] The invention relates to a double fluorescent PCR (polymerase chain reaction) detection primer, a probe, a reaction solution and a kit capable of rapidly detecting 16 respiratory pathogens, and belongs to the technical field of nucleic acid detection. Background technique [0002] Fever Respiratory Syndrome (FRS) is one of the most important group of infectious diseases in my country at this stage. Through the implementation of the major scientific and technological projects of 'Prevention and Treatment of Major Infectious Diseases such as AIDS and Viral Hepatitis' during the Eleventh Five-Year Plan and the Twelfth Five-Year Plan, my country has established a nationwide The interdepartmental and interdisciplinary infectious disease prevention and control support technology platform has realized the improvement of the emergency response capability of infectious diseases from point to point. my country's febrile respiratory syndrome monitoring program (20...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12Q1/04C12N15/11C12R1/93C12R1/35C12R1/01
CPCC12Q1/6858C12Q2531/113C12Q2537/143C12Q2561/101C12Q1/701
Inventor 史蕾雷湘华刘君叶颖徐云庆郭永超叶健忠辛本强
Owner SHENZHEN INT TRAVEL HEALTHCARE CENT
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