14 results about "Respiratory pathogens" patented technology

The application discloses a kind of absolute quantitative detection method of child respiratory tract multi-pathogen based on multi-target ladder microarray and exogenous internal reference correction.The method sets up independent micro-reaction unit to different pathogen, adds fixed amount of exogenous internal reference nucleic acid, and calculates template number by positive unit proportion combined with Poisson distribution model, then corrects the result by using the theoretical addition amount of exogenous internal reference, so as to realize parallel detection and absolute quantification of pathogen such as bocavirus, adenovirus, mycobacterium tuberculosis and mycoplasma pneumoniae without standard curve and special digital PCR equipment.The application has the advantages of high sensitivity, strong anti-interference ability, suitable for mixed infection sample analysis and clinical application, etc.

This utility model discloses a respiratory suction device, relating to the field of respiratory medicine technology. It includes a device base plate, with an annular fixing assembly fixedly connected to the top of the base plate. A suction container is inserted inside the annular fixing assembly, and a protective cover is inserted into the top of the suction container. A sealing ring is inserted inside the protective cover. Through the sealing ring and the filtration and purification assembly, this utility model achieves safety and filtration effects during operation. The filtration and purification assembly uses multi-layer filtration. The first layer, a polypropylene membrane, with its large pore size, quickly captures larger particulate impurities in the sputum. Two subsequent layers of cellulose filter cotton provide deep purification, intercepting tiny bacteria, viruses, and harmful aerosols. It can filter a large number of respiratory pathogens, greatly reducing the risk of cross-infection in wards. The sealing ring at the bottle opening is made of highly elastic, aging-resistant medical silicone, effectively preventing leakage and improving safety.

The application discloses a kit for detecting 87 respiratory tract pathogens and drug-resistant genes by using a single tube and application thereof. The kit comprises specific primers and probes for the 87 respiratory tract pathogens and drug-resistant genes and is based on a capillary electrophoresis platform. The kit can detect nearly 100 targets and has the advantages of high throughput, high sensitivity, high specificity, simple operation, short TAT time, simple result interpretation and the like, and is suitable for rapid screening and clinical diagnosis of respiratory tract pathogens and drug-resistant genes.

The application relates to the field of biological medicine, and discloses application of a GOSR1 gene in prevention and / or treatment of respiratory pathogenic bacteria. By specifically knocking down the GOSR1 gene, the application can reduce the expression level of pathogenic gene and protein, thereby significantly inhibiting the proliferation of the respiratory pathogenic bacteria. In addition, it is disclosed that overexpression of the GOSR1 gene can activate the proliferation of the pathogenic bacteria in cells infected by the respiratory pathogenic bacteria. The application provides a brand-new strategy for treatment of respiratory pathogenic bacteria infection based on the regulation of the GOSR1 gene on the proliferation of the respiratory pathogenic bacteria, and is especially suitable for intervention of influenza A virus infection.

The application provides a use of a pretreatment reagent composition for preparing a mass spectrometry detection kit for detecting respiratory pathogens, the pretreatment reagent composition comprising all reagents for mass spectrometry detection, such as a nasal swab preservative, a mass spectrometry matrix, a mass spectrometry calibrant, a quality control product, etc. The detection object of the application is a nasal swab sample, sampling is convenient, interference is small, and the pretreatment reagent composition can quickly inactivate and keep the sample for a long time, so that a variety of respiratory pathogens can be quickly, accurately and high-throughput mass spectrometry detected by cooperating the reagent composition with an existing detection reagent or kit.

This application belongs to the field of molecular detection technology, specifically relating to a detection kit for respiratory pathogens and drug resistance genes based on a capillary electrophoresis detection platform and its application. The kit simultaneously detects the pathogens infecting patients with respiratory tract infections and the possible drug resistance genes through a single tube reaction. Compared with traditional methods, this application covers common respiratory pathogens and drug resistance genes, with more comprehensive detection sites, higher specificity, better sensitivity, and stronger reliability, and has the capability for batch detection.

This invention relates to the field of biomedical technology, and more particularly to primer-probe combinations for broad-spectrum screening of common respiratory pathogens and their applications. Through screening and optimization, this invention has obtained a primer-probe combination for nucleic acid detection of respiratory pathogens for broad-spectrum screening of common acute respiratory infections and community-acquired pneumonia. The primer-probe combination described in this invention covers 18 respiratory pathogens and possesses advantages such as high accuracy, good specificity, strong anti-interference ability, good inclusiveness, high sensitivity, and superconductivity. Combined with fully enclosed single-tube PCR amplification, it can achieve parallel detection of 20 targets, greatly improving the early diagnosis of respiratory diseases with complex pathogen structures, and is of great significance for the prevention and treatment of infectious diseases.

The present application relates to the technical field of microfluidics, and particularly relates to a kit for rapidly detecting 15 respiratory pathogens and a use method thereof.The kit comprises a plurality of reaction cavities, each of which has a primer group corresponding to 15 respiratory pathogens; the primer group comprises inner primers, outer primers and loop primers for PCR amplification of Mycoplasma pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Haemophilus influenzae, Streptococcus pneumoniae, Escherichia coli, Legionella pneumophila, Burkholderia, Staphylococcus aureus, Corynebacterium striatum, Aspergillus, Pneumocystis jirovecii and Candida albicans.The present application can simultaneously detect 15 respiratory pathogens by adding samples once, avoids adding liquid reagents to multiple detection tubes, reduces the intensity of manual operation and the risk of mismatch.

The application belongs to the field of biological medicine, and particularly relates to a multiplex detection lower respiratory tract pathogen counterflow amplification primer set, probe and kit. The counterflow amplification primer set comprises a Legionella pneumophila primer pair, an adenovirus primer pair and a Chlamydophila psittaci primer pair. The primer sequences in the Legionella pneumophila primer pair, the adenovirus primer pair and the Chlamydophila psittaci primer pair each comprise a target nucleic acid complementary sequence and an interface sequence. The interface sequence is located at the 5' end of the target nucleic acid sequence and is not complementary to the target nucleic acid. The target nucleic acid complementary sequence has a folding sequence, and the folding sequence is complementary to the interface sequence, so that the primer sequence has a curved hairpin structure. A primer probe is designed, and a respiratory tract pathogenic bacteria multiplex detection kit is prepared. The multiplex detection can avoid non-specific amplification and improve the detection sensitivity.

The invention belongs to the technical field of molecular detection, and particularly relates to a respiratory tract pathogen detection kit based on capillary electrophoresis and application of the respiratory tract pathogen detection kit, the kit can detect common pathogens which are possibly infected by respiratory tract patients and cannot be detected and determined through a conventional technology or a kit, and the kit at least has the technical advantages of being high in sensitivity and specificity.

This invention belongs to the field of molecular diagnostics technology, specifically relating to primers, probes, and detection methods for CRISPR-Cas12a2 used in the detection of H1N1 influenza A virus. Targeting the conserved M gene of H1N1 influenza A virus, this invention designs a specific RT-PCR amplification primer set and crRNA, constructing an RT-PCR combined with CRISPR-SuCas12a2 fluorescence detection system. This system can stably detect as low as 5 copies / reaction of viral nucleic acid, achieving 100% specificity for common respiratory pathogens. The entire detection process takes no more than 90 minutes, and 96.19% of positive samples can be interpreted within 5 minutes. Testing of 205 samples showed that this invention has a total concordance rate of 91.22% with real-time quantitative PCR, exhibiting superior sensitivity and making it suitable for rapid screening and on-site immediate detection of influenza virus.

This invention belongs to the biomedical field, specifically relating to a convective amplification primer set and probes for detecting multiple respiratory pathogens and their applications. The convective amplification primer set includes an upstream primer and a downstream primer. Both the upstream and downstream primers include a complementary sequence and a tailing sequence complementary to the target nucleic acid. The tailing sequence is located at the 5' end of the complementary sequence and is not complementary to the target nucleic acid. The complementary sequence contains a folded sequence, which is complementary to the tailing sequence, giving the primer sequence a curved hairpin structure. The target nucleic acid is a specific nucleotide sequence derived from influenza A virus, influenza B virus, and Mycoplasma pneumoniae. Primer probes were also designed, and a kit for detecting multiple respiratory pathogens was prepared. This kit can avoid non-specific amplification and improve detection sensitivity.

The application relates to the field of biological medicine, and discloses application of a VTI1A gene, in particular, application of the VTI1A gene in prevention and / or treatment of respiratory pathogenic bacteria. By specifically knocking down the VTI1A gene, the application can reduce the expression level of pathogenic gene and protein, thereby significantly inhibiting the proliferation of the respiratory pathogenic bacteria. In addition, it is disclosed that overexpression of the VTI1A gene can activate the proliferation of the pathogenic bacteria in cells infected by the respiratory pathogenic bacteria. The application provides a brand-new strategy for treatment of respiratory pathogenic bacteria infection based on the regulation of the VTI1A gene on the proliferation of the respiratory pathogenic bacteria, and is especially suitable for intervention of influenza virus A infection.