99 results about "Respiratory pathogens" patented technology

The invention discloses a respiratory pathogen multi-detection reagent kit. The respiratory pathogen multi-detection reagent kit has the advantages that the respiratory pathogen multi-detection reagent kit is based on multi-PCR (polymerase chain reaction) technologies, detection results can be determined by the aid of fluorescence resonance energy transfer via the melting temperature ranges, the respiratory pathogen multi-detection reagent kit can be used for qualitatively simultaneously detecting 16 types of respiratory pathogens, the 16 types of respiratory pathogens include 12 types of RNA(ribonucleic acid) viruses (influenza A viruses, influenza B viruses, H1N1 influenza A viruses, type A and type B respiratory syncytial viruses, type -1 / -2 / -3 parainfluenza viruses, type OC43 coronaviruses, type 229E coronaviruses, rhinoviruses and human metapneumovirus), 2 types of DNA (deoxyribonucleic acid) viruses (adenoviruses and bocavirus) and 2 types of bacteria (mycoplasma pneumoniae andbordetella pertussis), the respiratory pathogen multi-detection reagent kit is high in detection sensitivity, and the sensitivity even can reach 1 copy / reaction; the multi-detection reagent kit is good in specificity, and negative results of pathogens which have identical sampling sites and similar pathogenic mechanisms and are not in the detection range of the respiratory pathogen multi-detectionreagent kit can be obtained; the respiratory pathogen multi-detection reagent kit is short in operation time and easy to operate and can be used for quickly detecting the 16 types of respiratory pathogens in a single tube of a reaction system, the results are clear and are easy to interpret, and the like.

The invention relates to a primer composite for detecting respiratory pathogens. The primer composite comprises at least one group of a mycoplasma pneumoniae group, a chlamydia pneumoniae primer group, an influenza A / B virus primer group, a parainfluenza virus primer group, an adenovirus primer group and a respiratory syncytial virus primer group. The invention further relates to a kit comprising the primer composite. The kit further comprises a micro-fluidic chip wrapping primers, and a macroscopic indicator. The invention further relates to a detection method adopting the primer composite. The detection method comprises the steps of primer composite coating, to-be-detected sample nucleic acid extraction, LAMP reaction and visual result interpretation. The kit and the method are applied to the micro-fluidic chip for visual judgment, instant detection of the seven respiratory pathogens is rapidly and accurately realized, and a result is judged by a naked eye by color change, so that the kit and the method are simpler, more convenient and quicker in practical applications, are easy to operate, and are suitable for site operation.

The invention discloses a method for detecting 29 respiratory pathogens by using a Taqman low-density microfluidic chip (TAC) technology. According to the method, the TAC detection technology is established for the 29 respiratory pathogens, the advantages of MGB probe detecting are fully used, the defects of previous multiple detection of the respiratory pathogens are overcome, 29-respiratory-pathogen multi-target detection of the respiratory pathogens can be achieved on a sample in two hours, and the good sensitivity and the good specificity are achieved. According to the method for detectingthe 29 respiratory pathogens by using the TAC technology, a quick, accurate and high-throughput technological mean is provided for monitoring and outbreak investigation of respiratory diseases in China.

The invention discloses a probe and primer composition for rapidly detecting seven coronaviruses and other respiratory tract pathogens. A group of specific primers and the Taqman or MGB probe are designed mainly according to the specificity of N genes of OC43-CoV, NL63-CoV, 229E-CoV, HKU1-CoV, SARS-CoV, FluB and RSV, ORF1a, ORF1b and N genes of MERS-CoV, ORF1ab and N genes of 2019-nCoV, an M geneof FluA and an HEXON gene of HadV, and by utilizing a melting curve technology, CoV-OC43, HCoV-NL63, HCoV-229E, HCoV-HKU1, SARS-CoV, MERS-CoV, 2019-nCoV, FluA, FluB, HAdV and RSV are rapidly identified according to the change of the melting temperature. By means of the design, multiple pathogens can be detected in one fluorescent channel at the same time, and a relatively high sensitivity can be met.

The invention provides a primer group capable of simultaneously detecting 27 respiratory tract pathogens based on a nucleic acid mass spectrometry technology, a kit and application, and belongs to thefield of pathogen nucleic acid detection. According to the kit, PCR amplification primers and single-base extension primers are designed for 27 clinically common respiratory tract pathogens. Pathogenic bacteria corresponding primer and single base extension primer sequences are shown in SEQ ID NO: 1-81. According to the nucleic acid mass spectrometry, a multiple RT-PCR technology and a single base extension technology are integrated, pathogen target genes are amplified through multiple RT-PCR, and the detection sensitivity is improved; in the single-base extension reaction, one base amplification is carried out on an extension primer, and pathogen identification is carried out by distinguishing the molecular mass of an extension product, so that the specificity is high. Meanwhile, the flux of the nucleic acid mass spectrum is high, amplification of 40 genes can be achieved at most through one reaction hole, and 384 samples can be analyzed at most at a time by means of the chip technology; the kit has the advantages of high automation degree, high detection flux, high sensitivity and good specificity.

Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

The invention provides a primer, product and method for detecting feline respiratory tract pathogens as well as application, and relates to the technical field of nucleic acid tests. In the primer group for detecting the feline respiratory tract pathogens, a primer for detecting feline herpesvirus type 1 (FHV1), a primer for detecting feline calicivirus (FCV), a primer for detecting mycoplasma, aprimer for detecting feline chlamydia and a primer for detecting bordetella bronchiseptica are primers designed through highly conservative regions of each pathogen, have no cross reaction with otherfeline pathogens, and have the characteristics of high specificity and high sensitivity. By use of the primer group, five feline respiratory tract pathogens including the FHV1, the FCV, the mycoplasma, the feline chlamydia and the bordetella bronchiseptica can be identified in the same reaction system, and the detection cost and time are greatly reduced as compared with a traditional method.

The invention relates to a composition for detecting 23 respiratory pathogens. The composition comprises a primer composition consisting of the sequences shown by SEQ ID NO. 1-SEQ ID NO. 48 and a probe composition consisting of the sequences shown by SEQ ID NO. 49-SEQ ID NO. 72. The invention also relates to a kit for detecting 23 respiratory pathogens and a detection method of the kit. The kit comprises a first reaction solution, a second reaction solution, a first hybridization solution and a second hybridization solution containing the primers and probes respectively; the kit also comprises a mixed enzyme, a streptavidin-phycoerythrin solution, a negative reference, a positive reference and an interior label. The kit and the detection method provided by the invention integrate the functions of RT-PCR, full-automatic liquid-phase chip hybridization detection, data analysis and the like, and also have the advantages of high throughput, multiple detection target spots, high degree of automation, easiness in operation, short time consumption among the inventions of the same kind of liquid-phase chip technologies, etc.; moreover, the kit and the detection method provide important information for addressing the respiratory tract infection epidemic in time, and are of great significance to the prevention and control of respiratory pathogens.

A non-invasive method for rapidly determining the specific presence or absence of respiratory pathogens and chemical entities.

The invention discloses a respiratory tract pathogen detecting kit. The kit is used on the basis of multiple PCR combining nucleic acid intrusion reaction and the nano-gold color developing principle. The kit comprises one or more of 6 primer probe combinations and can be used for detecting influenza A / avian influenza viruses (FluA), influenza B viruses (FluB), SARS coronaviruses, legionella pneumophila (LP), neisseria meningitidis (N. men) and adenoviruses (ADV). The kit has the advantages that the kit is high in specificity and sensitivity during respiratory tract pathogen detecting, expensive equipment is not needed, detecting results can be observed through naked eyes, and the kit can be applied at primary level.

The invention discloses a kit for detecting respiratory adenoviruses, streptococcus pneumoniae and bordetella pertussis based on a micro-fluidic chip and a use method of the kit. The kit comprises a multi-flux micro-fluidic nucleic acid detection chip for actively controlling flow paths, a pre-filled dry powder detection reagent and a positive quality control product, wherein the pre-filled dry powder detection reagent contains primers with specific conserved sequences for the respiratory adenoviruses, the streptococcus pneumoniae and the bordetella pertussis and a TaqMan fluorescent probe. The kit disclosed by the invention adopts a micro-fluidic chip technology, all operation processes are completed by instruments after application of a sample, the operation is simple and convenient, thespeed is high, the detection can be completed within 30-60 min, and pollution can be avoided; the fluorescent PCR technology of the TaqMan probe is adopted to detect the respiratory adenoviruses, thestreptococcus pneumoniae and the bordetella pertussis; the sequences of the designed primers and probe are very conserved in genes of the respiratory adenoviruses, the streptococcus pneumoniae and the bordetella pertussis, and the designed primers and probe have high specificity; and detection results can be obtained within 2h, and the sensitivity can reach 10 copies / mu L.