9 results about "Canine distemper" patented technology

The invention discloses a cat kidney suspension culture cell line suitable for serum-free suspension culture and application of the cat kidney suspension culture cell line. The name of the cell line is F81-B4, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.46350. After 50 generations of continuous subculture, the cells still can maintain the original proliferation level and cellular morphology, the cell number can reach 9.0 * 10 < 6 > / mL after the cells are subcultured at the density of 1.0 * 10 < 6 > / mL for three days, the multiplication time is 22-26 hours, and the cell proliferation speed is equivalent to that in a shake flask when the cells are amplified to a 10L bioreactor for culture. The cell line is highly susceptible to feline parvovirus, feline calicivirus, feline herpes virus, canine parvovirus and canine distemper virus, the virus content of a virus solution harvested 72 h after FPV virus inoculation can reach 107.5 TCID50 / mL, the virus content of a virus solution harvested 24 h after FCV virus inoculation can reach 1010.8 TCID50 / mL, the virus content of a virus solution harvested 48 h after FHV-1 virus inoculation can reach 107.5 TCID50 / mL, compared with an existing F81 adherent cell production process, the production process has the advantages that the production efficiency is high, and the production cost is low. The method has the characteristics of rapid lesion, short virus collection time and high titer. The cell matrix is an ideal cell matrix which can be used for culturing the three viruses.

The invention discloses a primer group and a kit for simultaneously detecting a panda aleutian virus and a panda canine distemper virus Asia type 6 and application of the primer group and the kit, and belongs to the technical field of virus detection of endangered wild animals. The invention provides a primer group and a kit for specifically recognizing the aleutian disease virus and the canine distemper virus Asia 6 type of the panda, and detection can be completed within 20 minutes under the condition of constant temperature of 39 DEG C; according to the present invention, the method has characteristics of rapid detection, good specificity, high sensitivity and the like, does not produce the cross reaction with other common pathogens, does not produce the false positive phenomenon due to the generation of the primer dimer, has characteristics of rapid detection, good specificity, high sensitivity and the like, and has characteristics of simple and easy operation, and on-site detection without special detection personnel; the method is rapid and accurate in detection, has a good application effect in preliminary application, and provides a method for rapid diagnosis, virus traceability, epidemic disease monitoring and early warning of a wild animal epidemic disease source and the like in zoos and wild animal rescue institutions.

This invention discloses a primer set, reagent kit, and their applications for the simultaneous detection of Aleutian virus and Canine Distemper Virus Asia 6 (Asia 6) in red pandas, belonging to the field of endangered wildlife virus detection technology. This invention provides a primer set and reagent kit that specifically identifies Aleutian virus and Canine Distemper Virus Asia 6 in red pandas. Detection can be completed within 20 minutes under constant temperature conditions of 39℃; it does not cross-react with other common pathogens and does not produce false positives due to primer dimer formation. In other words, it features rapid detection, high specificity, and high sensitivity. The implementation process is simple and easy to operate, requiring no specialized testing personnel for on-site testing. The detection is rapid and accurate, and initial applications have shown good results, providing a method for rapid diagnosis, virus tracing, wildlife disease monitoring, and early warning in zoos and wildlife rescue institutions.

The present invention provides a traditional Chinese medicine composition for treating neurological symptoms of canine distemper and a preparation method thereof. The traditional Chinese medicine composition comprises the following raw medicinal materials in parts by weight: 5-10 parts of donkey-hide gelatin, 2-20 parts of tortoise shell glue, 2-10 parts of turtle shell glue, 2-10 parts of white atractylodes, 2-10 parts of ophiopogon, 5-15 parts of white peony root, 5-15 parts of poria, 5-15 parts of ginger, 5-15 parts of evodia, 5-15 parts of arisaema, 5-15 parts of calamus, 5-10 parts of wolfberry, 2-10 parts of dodder seed, and drynaria rhizome. The traditional Chinese medicine composition of the present invention has the functions of calming the liver and suppressing yang, calming wind, nourishing the liver and kidney, clearing away heat and calming convulsions, and treating epilepsy and convulsions. It has a good therapeutic effect on the neurological symptoms such as muscle twitching, circling, bumping, foaming at the mouth, clenched jaw, falling to the ground and convulsions caused by wind-heat internal movement caused by canine distemper, depletion of body fluids, heat toxins entering the blood, and liver-wind internal movement, which are manifested by convulsions.

The present invention provides a primer set for multiplex PCR for the simultaneous detection of four pathogens, a detection method, and a kit thereof, and relates to the field of biological detection technology. The present invention has developed and designed a rapid nucleic acid extraction method for four common pathogens in living minks and a primer set for multiplex PCR detection. The primer set includes upstream and downstream primers for detecting mink canine distemper virus, parvovirus, Aleutian virus, and Pseudomonas aeruginosa, respectively. The nucleic acid extraction method provided by the present invention is simple and rapid to operate, and has a good extraction effect; the primer set, detection system, and detection method for multiplex PCR detection are characterized by high efficiency, strong sensitivity, good specificity, and good repeatability, and overcome the cumbersome steps of single-plex PCR, are easy to operate, simple and efficient, and have pioneering significance.

The invention discloses a probiotic fermented traditional Chinese medicine composition for preventing and controlling canine distemper and a preparation method of the probiotic fermented traditional Chinese medicine composition, and belongs to the technical field of fermented compositions. The feed additive is prepared from the following raw materials in parts by weight through fermentation: 100 to 200 parts of cortex phellodendri, 10 to 20 parts of radix aucklandiae, 100 to 200 parts of liquorice root, 200 to 400 parts of herba taraxaci, 100 to 200 parts of rhizoma coptidis and 10 to 40 parts of a microbial preparation. The microbial preparation comprises bacillus licheniformis SXDYYB15, the bacillus licheniformis SXDYYB15 is preserved in the China Center for Type Culture Collection on April 18, 2025, and the preservation number of the bacillus licheniformis SXDYYB15 is CCTCC (China Center for Type Culture Collection) NO: M2025814. The composition breaks through the bottleneck that the traditional Chinese medicine takes effect slowly through a triple synergistic mechanism of traditional Chinese medicine antivirus, probiotic immunoregulation and gastrointestinal function repair; on the premise of adapting to the canine intestinal environment, the pharmacodynamic bioavailability is remarkably improved through directional fermentation, and an efficient, safe and economical solution is provided for prevention and control of canine distemper.

The present invention provides a recombinant canine distemper virus vector comprising a heterologous polynucleotide encoding an avian influenza antigen. The recombinant viral vectors are suitable for use in immunogenic compositions and vaccines that can provide protection against avian influenza and other pathogens when administered to animals.

A giant panda canine distemper virus detection kit based on multi-enzyme isothermal nucleic acid rapid amplification technology and a use method are provided. For CDV, the kit selects virus conserved gene, designs specific primers in the conserved region of the gene, amplifies the N gene fragment of CDV by multi-enzyme isothermal rapid amplification technology, and then detects the amplified products with nucleic acid colloidal gold test strips to establish a MIRA detection method for rapid auxiliary diagnosis with CD.

The application discloses a canine distemper detection card with a safety protection structure, which comprises a shell, a sample adding hole is formed in the top of the shell, an observation groove is formed in the top of the shell on one side of the sample adding hole, a cavity is formed in the shell, a detection line and a quality detection line are arranged on a detection test paper, and a mark is marked in English on the top of the shell on one side of the observation groove. The canine distemper detection card with the safety protection structure is provided with a safety protection assembly, a rotating column, a cover plate and an extension column are arranged in the safety protection assembly, the cover plate is rotated, the cover plate is closed or opened by rotating the rotating column to close or open a detection part, when the extension column is pressed downward, the extension column is extended and retracted to drive a fixing ring and a third extension rod to be extended and retracted in a first cylindrical groove, the third extension rod is extended and retracted to extrude a limiting block in the limiting hole, meanwhile, the limiting block on one side of the extension column and the cover plate are rotated to the other side under the elasticity of a first spring on one side, so that the sample adding hole is opened, and a sample is added into the sample adding hole for detection.