80 results about "Canine distemper" patented technology

The invention provides a Chinese medicinal composition for treating canine distemper and a preparation method thereof, and relates to a medicament for treating the canine distemper. The Chinese medicinal composition is prepared by mixing isatis indigotica fort, great burdock achene, Chinese pulsatilla root, astragalus, gambir plant, honeysuckle flower, platycodon root, weeping forsythiae capsule, liquoric root, baical skullcap root, indigowoad leaf, kudzuvine root, rhizoma arisaematis cum bile, halloysit and golden thread; mixing raw materials; adding water to soak and decoct the mixture for certain time; and filtering the mixture. The composition has the advantages of wide materials, low cost, safety, effectiveness, no medicament resistance and convenient use, and has the effects of clearing heat and detoxicating, clearing liver and improving vision, moistening lung and transforming phlegm, cooling blood and checking dysentery, extinguishing wind and resolving tetany, upbearing the clear and downbearing the turbid, and strengthening organic immunologic functions.

The invention relates to a yolk antibody injection against canicola fever and canine parvovirus and a preparation method thereof. The yolk antibody injection against canicola fever and canine parvovirus is extracted and prepared by the collected yolk antibody of the immunized laying hens which are immunized by the bivalent vaccine of the canicola fever and canine parvovirus. The yolk antibody injection against canicola fever and canine parvovirus prevents the diseases by improving the antibody level of canine bodies to the canicola fever and the canine parvovirus. The yolk antibody injection against canicola fever and canine parvovirus and a preparation method thereof have the beneficial effect that the anti-canicola fever and canine parvovirus disease egg yolk antibody injection is provided with faster effect in the onset of canicola fever and canine parvovirus diseases. The yolk antibody injection against canicola fever and canine parvovirus and the preparation method thereof has the advantages of obvious efficacy, low cost, fast effect, no side effects, no generated drug resistance, simple preparation, wide raw material resource, convenient use and easy application.

The present invention discloses a hybridoma cell line and an anti-canine distemper virus N protein monoclonal antibody CDV-1N8 produced through the hybridoma cell line, and belongs to the field of CDV prevention and treatment. According to the present invention, the hybridoma cell line stably secreting the anti-CDV N protein monoclonal antibody is screened, wherein the microorganism preservation number is CGMCC No.8793, and experiment results prove that the monoclonal antibody CDV-1N8 secreted by the hybridoma cell line can specifically react with the CDV N protein, and does not react with the Vero cell protein; the monoclonal antibody can specifically recognize the CDV-N protein, and accurate positioning of the CDV-N protein B-cell epitope recognized by the monoclonal antibody is QITFLHSERS; and the monoclonal antibody CDV-1N8 and the CDV N protein virus-specific conservative B cell epitope polypeptide recognized by the monoclonal antibody can be made into the CDV infection diagnosis reagent so as to establish the foundation for establishment of the CDV serological differential diagnosis method.

The invention relates to the field of veterinary biological products and particularly relates to a preparation method of a mink canine distemper virus live vaccine. The method comprises the following steps: inoculating a bioreactor with sensitive cells for vaccine preparation, and culturing by using a micro-carrier; after the sensitive cells are cultured by over 50% and grow into a dense single layer, inoculating the bioreactor with canine distemper virus for enrichment culture; harvesting the virus culture liquid and micro-carrier; performing freeze-thawing and removing the micro-carrier and cell debris to obtain virus liquid; and blending the virus liquid to obtain the vaccine. By improving the reaction conditions of each step and optimizing the production flow, the method provided by the invention realizes the technical effects of short production cycle, high virus titer, stable product quality, increased production efficiency and low side effect.

The invention discloses a two-color fluorogenic quantitative PCR (Polymerase Chain Reaction) detection kit for CDV (canine distemper viruses) and CPV (canine parvo viruses). A detection method comprises the following steps: firstly, pre-treating a sample by using a nucleic acid extraction solution of the kit and extracting nucleic acid; then detecting the nucleic acids of the CDV and CPV simultaneously in a reaction system by utilizing the two-color fluorogenic quantitative PCR detection kit, and detecting whether the canine distemper and canine parvo viruses exist in the sample to be detected or not by one step; and quantifying a virus copy number. The detection method is high in detection efficiency and good in specificity and sensibility; the aims of clinical detection and epidemic situation control of animal epidemic diseases can be realized.

The invention provides a medicine capable of controlling feline distemper as well as a preparation method and applications of the medicine. The medicine capable of controlling feline distemper comprises a culture supernatant of human umbilical cord mesenchymal stem cells containing bioactive factors, recombinant cat omega-interferon and granulocyte colony-stimulating factors, wherein the molecularweight of the bioactive factors is 3-100 Kda. The medicine can effectively control diseases caused by feline distemper viruses and alleviate the symptoms including lassitude, vomiting, decrease of leukocytes, and the like which are caused by virus infection. The medicine provided by the invention can be transported for a long distance and stored for a long term, is convenient to use, belongs to aspecific medicine aiming at feline panleukopenia virus, and has a broad application prospect.

The invention relates to a preparation method for obtaining blood used for preparing homologous antiserums and a spleen byproduct used for preparing transfer factors from the bodies of fur bearing animals such as foxes, raccoon dogs and minks; the furs of the foxes, the raccoon dogs and the minks are taken concentratedly in December and confirmed according to the fur-taking dates of different areas; healthy individuals are picked up 45 days before a predicated fur-taking date; the booster immunization of univalent vaccines or polyvalent vaccines of canine distemper, Canine parvovirus enteritis, adenovirus disease, corona virus laxness, parainfluenza, and pasteurellosis is carried out for 3 times by purified and condensed antigens on the foundation of carrying out immunization for once in summer; when the furs are taken, aseptic anticoagulation blood collection is carried out to a heart to improve the blood serum yield; and the spleens of the animals are collected for extracting specific or common transfer factors simultaneously. The blood can be conveniently prepared into the blood serums which resist communicable diseases, and the transfer factors can be extracted the spleen, thus providing guarantee for the healthy development of the breeding in the next year; the invention is used for curing the corresponding communicable diseases of the wild animals of the same species; and when a small amount of communicable disease cases appear in a breeding crowd, the corresponding pathogeny hyper-immune serums and transfer factors are mainly injected to a supposed healthy crowd after the pathogeny is confirmed so as to cure the affected animals in the early period of disease and control the spreading of the epidemic situations.

The invention relates to a combination of a mink viral enteritis inactivated vaccine and a canine distemper live vaccine. The combination is the mink viral enteritis inactivated vaccine of using an alkylating agent as a virus inactivating agent, and is used as a diluent of the canine distemper live vaccine in the combination; and the two vaccines in the combination have a specific mixture ratio. Compared with traditional application methods of the two vaccines, the vaccine combination reaches the effects of dual prevention (preventing mink viral enteritis and canine distemper) in one injection, thus the vaccine cost is reduced, the animal stress reaction is reduced, the immune effect is improved, and the labor intensity of a feeder is relieved, thus obtaining significant economic benefits.

The invention provides a preparation method of a canine distemper/infectious hepatitis/parvovirus triple egg yolk antibody and provides the canine distemper/infectious hepatitis/parvovirus triple egg yolk antibody prepared with the preparation method. The preparation method comprises steps as follows: eggs laid by immunized layer hens are collected and subjected to surface sterilization, egg yolk is separated, homogenized and diluted with sterile distilled water, pH is regulated to 5.0-5.2, a mixture is left to stand, and a supernatant is collected; the supernatant is diluted with an acetate buffer solution, pH is regulated to 5.0-5.2, n-caprylic acid is added, a mixture is left to stand for layering, a water phase is taken and centrifuged, precipitates are taken and dissolved in normal saline, filtering is performed, and the egg yolk antibody is obtained. The egg yolk antibody has the advantages of stable physicochemical properties, wide sources, high output and low cost as well as large animal phylogenetic distance, is more suitable for producing specific antibodies, can be used for preparing a diagnostic reagent, controlling diseases and serving as a feed additive, and has the potential in development of functional foods and new drugs.

The invention relates to oral liquid for treating canine distemper. The oral liquid is prepared by mixing and extracting the following components in parts by weight: 15 to 35 parts of sanguisorba officinalis, 15 to 25 parts of flos sophorae, 15 to 35 parts of herba taching, 15 to 25 parts of rhizoma coptidis, 15 to 25 parts of radix curcumae, 15 to 35 parts of fructus mume, 20 to 40 parts of honeysuckle, 10 to 20 parts of angelica, 15 to 25 parts of liquorice, and 5 to 10 parts of ribavirin. The oral liquid has the beneficial effects of being reasonable in composition and simple to prepare, being capable of looking into both root causes and symptoms by combination of traditional Chinese medicines and western medicines, achieving high biological availability, having a greatly remarkable curative effect, and having a special effect on treating the canine distemper according to clinical evidence.

Methods and compositions are provided to treat enteric disease. The methods and compositions use passive immunity to treat diseases of the gut, including diseases caused by Salmonella sp., E. Coli, Clostridium difficile, Clostridium perfringens A, Clostridium perfringens C, Clostridium perfringens D, canine distemper, feline distemper, Rotavirus, and Parvovirus. Specific antibodies are administered orally to a subject to improve intestinal health.

The invention provides a traditional Chinese medicine composition for treating canine distemper, which comprises the following components: radix gentianae, scutellaria baicalensis, Phellodendron amurense, honeysuckle, forsythia suspense, houttuynia cordata, gypsum, rhizoma anemarrhenae, fructus sophorae, lalang grass rhizome, anemone chinensis and liquorice. The traditional Chinese medicine composition is decocted with water according to the proportion in the prescription, and then is drenched to sick dogs. The traditional Chinese medicine composition adopts the traditional Chinese medicine formula, can treat the canine distemper effectively, is low in cost, and has no side effects.

The invention discloses a therapeutic antibody for feline distemper. The therapeutic antibody for feline distemper is prepared from an FPV inactivated vaccine and an FPV subunit vaccine as immunogensby horse immunization. Therefore, a corresponding preparation method is established; according to the method, two different types of FPV immunogens are creatively inoculated to a healthy horse according to a certain program, the FPV inactivated vaccine is taken as a basic immunogen, the FPV subunit vaccine is taken as an enhanced immunogen, hyperimmune blood is collected after a high-potency antibody is produced, and high-activity immune globulin F(ab')2 is prepared by IgG purification and Fc' segment excision. With the application of the method, a large amount of horse-derived anti-FPV hyperimmune serum can be prepared, and the high-potency F(ab')2 antibody is extracted and purified. A test indicates that the prepared therapeutic antibody for feline distemper not only has higher potency,but also has exact feline distemper preventing and treatment effects, can be used for emergency prevention and treatment of feline distemper and has important practical significance and broad application prospect.

The invention discloses a method for establishing a canine distemper sensitive cell line based on a Nectin4 receptor. The method comprises the following steps: (1) extracting total RNA of a sample andsynthesizing cDNA; (2) amplifying and cloning Nectin4; (3) modifying Nectin4; (4) connecting and converting dNectin4-Hatag and extracting recombinant plasmid; (5) packaging recombinant slow virus; abd (6) transfecting cells and screening positive cell line. The Vero-Nectin4 established according to the method disclosed by the invention can stably express the Nectin4 receptor and is high in universality; the CDV virulent strain is capable of growing in the cell line and acquiring obvious CPE lesion; and the method is suitable for wide application in culturing or separating the canine distempervirus.

The invention provides a canine interferon-alpha6 alpha7 recombinant protein and a preparation method and an application thereof. An amino acid sequence of the canine interferon-alpha6 alpha7 recombinant protein is shown as SEQ ID NO:6, the preparation method comprises the following steps: performing codon optimization on a canine interferon-alpha6 gene and a canine interferon-alpha7 gene, splicing the optimized gene sequence, cloning the gene to pPICZalphaA for construction of recombinant expression plasmids, converting pichia yeast, and preparing recombinant bacteria, and performing steps offermentation, inducible expression, purifying to obtain the canine interferon-alpha6 alpha7 recombinant protein. The canine interferon-alpha6alpha7 recombinant protein can be used as a wide spectrumantiviral preparation, has obvious prevention and treatment effects for canine distemper, canine parvovirus disease, canine parainfluenza virus infection, and canine infectious hepatitis virus infection, and provides an effective technical means for realizing recombinant canine interferon-alpha6 alpha7 series-connection recombinant protein production and further broad-spectrum prevention and canine viral epidemic disease treatment.

The invention discloses a feed additive for treating self-biting disease of minks and a preparation method thereof. The feed additive is prepared from the following ingredients: acanthopanax root, mothers of pearl, dens draconis, goat horns, Ludisia discolor, radix paeoniae rubra, cynanchum atratum, spina gleditsiae, eucalyptus leaves, citrus aurantium, red roses, flowers of silktree albizzia, Cortex Sophorae, radix astragali, red halloysite, honey, pine needle meal, shrimp meal, rice bran, silkworm chrysalis, fish scale and olive oil. The feed additive disclosed by the invention can be used for effectively treating canine distemper of the minks and is low in drug residue, good in treatment effect, high in cure rate and low in toxic or side effects, the cure recovery period is shortened, the food intake of the minks is increased, the use is convenient, and the economic benefit of farmers is increased.

The invention relates to a traditional Chinese medicine composite preparation for effectively controlling the fur animal viral diseases. The preparation is composed of the following nine traditional Chinese herbals by weight: 20 to 24 grams of radix astragali, 5 to 12 grams of white peony root, 10 to 12 grams of Chinese angelica, 8 to 12 grams of bighead atractylodes rhizome, 5 to 12 grams of poria cocos, 3 to 6 grams of cassia twig, 1 to 3 grams of Sichuan pepper, 8 to 10 grams of saposhnicovia divaricata, and 6 to 9 grams of licorice. The preparation method of the composite preparation comprises the following steps: selecting roots, stalks, and seeds of pure natural high-quality traditional Chinese herbals, individually grinding the selected traditional Chinese herbals by a traditional Chinese herbal grinder under a clean and dry condition, sieving the grinded traditional Chinese herbals by a 100-mesh sieve, and finally evenly mixing the processed traditional Chinese herbals according to the weight ratio mentioned above. The composite preparation can be used along with feed, and can also be orally taken after being decocted with water. The composite preparation has the advantages of user-friendliness, stable curative effect, no side or toxic effect, and no drug residue. The composite preparation is mainly used to prevent and treat viral diseases (canine distemper, viral enteritis, etc.) of fur animals such as mink, fox, racoon dog, and the like, and has a high practical and promotion value.

The invention discloses a multiple-FIA (fluorescence immunoassay) method and kit for quickly distinguishing CDV (canine distemper virus), CPV (canine parvovirus) and RV (rabies virus). The operation is simple, a target amplified fragment is obtained through a PCR (polymerase chain reaction), then an amplification product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, the MFI (mean fluorescence intensity) value is read through a detector, and different types of pathogens are distinguished. The method can be used for detecting and distinguishing the CDV, CPV and RV simultaneously, has the advantages of high specificity, high sensitivity, good repeatability and the like and can realize simultaneous detection of various different target molecules in the same sample. The method is good in flexibility, and the type of detected pathogens can be increased or decreased on the basis as required.

The invention discloses an ELISA detection kit for a canine distemper and canine parvovirus antibody. The ELISA detection kit comprises an ELISA slat, a serum diluent, a concentrated detergent, a substrate developing solution, a stop solution, an enzyme-labelled monoclonal antibody, a positive control, a negative control, and a horseradish peroxidase conjugated-rabbit anti-dog IgG polyclonal antibody. The ELISA detection kit for the canine distemper and canine parvovirus antibody is convenient and efficient, and a serum antibody is detected through serum to judge the canine immune protection condition after vaccination.

The invention discloses a vaccine for expressing panda canine distemper virus H and F gene recombinant goat pox virus.The vaccine comprises recombinant goat pox virus vpTK-H-Eg or vpTK-F-Eg obtained by using goat pox virus as a vector to transfect and express respectively recombinant transfer vector plasma pTK-H-Eg or pTK-F-Eg of panda canine distemper virus H or F gene, wherein the recombinant goat pox virus vpTK-H-Eg comprises amino acid sequences shown as in SEQ ID NO.1, and the recombinant goat pox virus vpTK-F-Eg comprises amino acid sequences shown as in SEQ ID NO.2.The vaccine developed herein has the advantages of safety, high efficiency, low production cost and the like, and basis is laid for the prevention and treatment of canine distemper.

A process for preparing the leukocyte interferon of dog for preventing and treating canine distemper, canine parvovirus disease, etc includes using chicken Newcastle disease virus (DNV) to induce the leukocyte of health dog, culture, deactivating virus, removing bacteria, separation, purifying and freeze drying to obtain powder injection.

The invention provides a canine distemper genetic engineering subunit vaccine and a preparation method and application thereof. The canine distemper genetic engineering subunit vaccine comprises a vaccine adjuvant and an antigen. The antigen is an H protein having an amino acid sequence shown in the formula of SEQ ID NO: 2. The canine distemper subunit vaccine has stable biological characteristics, has good immunogenicity, is safe and reliable, has the very good protective effect on the leeches attacked by the canine distemper virulent GN strain, can effectively prevent the prevalence and spread of leech canine distemper after immunity, can reduce the economic losses caused by this disease and has broad application prospects.