This invention discloses a primer set,
reagent kit, and their applications for the simultaneous detection of Aleutian
virus and
Canine Distemper Virus Asia 6 (Asia 6) in red pandas, belonging to the field of endangered
wildlife virus detection technology. This invention provides a primer set and
reagent kit that specifically identifies Aleutian
virus and
Canine Distemper Virus Asia 6 in red pandas. Detection can be completed within 20 minutes under constant temperature conditions of 39℃; it does not cross-react with other common pathogens and does not produce false positives due to
primer dimer formation. In other words, it features
rapid detection, high specificity, and high sensitivity. The implementation process is simple and easy to operate, requiring no specialized testing personnel for on-
site testing. The detection is rapid and accurate, and initial applications have shown good results, providing a method for rapid diagnosis, virus tracing,
wildlife disease monitoring, and early warning in zoos and
wildlife rescue institutions.