125 results about "Colloidal au" patented technology

The invention relates to the technical field of molecular biology, in particular to an avian adenovirus serum type 4 colloidal gold test strip based on a monoclonal antibody and application of the test strip. According to the invention, two monoclonal antibodies (6B3 and 8G11) aiming at FAdV4 penton protein are successfully prepared through a hybridoma technology, and both the monoclonal antibodies show high titer and strong serotype specificity. Specific analysis proves that the two monoclonal antibodies can recognize 12 FAdV4 isolates from different sources, and do not have cross reaction with other FAdV serotypes or common poultry pathogens. According to the invention, through systematic optimization of key parameters, the detection sensitivity of the test strip is 7.81 * 10 < 4 > TCID50 / 0.1 mL, and the detection time is only 15 minutes. Clinical sample verification shows that compared with an ELISA method, the total coincidence rate is 98.6%.

The invention belongs to the technical field of colloidal gold immunochromatography, and relates to a cetirizine monoclonal antibody, a nucleic acid molecule, a carrier, a detection kit and application thereof. The cetirizine monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises a VH-CDR1, a VH-CDR2 and a VH-CDR3, the light chain variable region comprises a VL-CDR1, a VL-CDR2 and a VL-CDR3, the sequence of the VH-CDR1 is as shown in SEQ ID NO.1, the sequence of the VH-CDR2 is as shown in SEQ ID NO.2, and the sequence of the VH-CDR3 is as shown in SEQ ID NO.3. The invention further discloses a preparation method of the cetirizine monoclonal antibody. The monoclonal antibody provided by the invention has high sensitivity to cetirizine, the lowest detection limit is 0.007 ng / mL, the quantitative range is 0.017-0.282 ng / mL, and the specificity is strong.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

The invention belongs to the technical field of biological detection, and particularly relates to a preparation method of lipopolysaccharide-beta-1, 3-glucan binding protein and application of the lipopolysaccharide-beta-1, 3-glucan binding protein in preparation of a broad-spectrum colloidal gold detection product for dermatophytes. The prokaryotic expression system induces the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein to be efficiently expressed in an inclusion body form, the optimized inclusion body purification-renaturation process is combined to realize batch preparation, and the colloidal gold chromatography technology is integrated, so that the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein is obtained. The bottlenecks of the traditional detection method in sensitivity, broad spectrum and field applicability are broken through.

The invention discloses a deep learning-based colloidal gold test paper result analysis system for detecting renal injury molecular 1. The system comprises an image acquisition module, a region processing module, an effectiveness judgment module, a concentration analysis module and a result generation module, the image acquisition module is used for acquiring a visible light image of the kim-1 colloidal gold test paper, wherein the visible light image at least comprises a detection line area and a quality control line area, and carrying out gray standardization processing, contrast enhancement processing, edge sharpening processing and target area positioning processing on the visible light image; the region processing module is used for performing positioning and boundary segmentation to obtain a mask image which is consistent with the input image in size and is marked with the position of each functional region; the effectiveness judgment module is used for judging whether the kim-1 colloidal gold test paper is effective or not; the concentration analysis module is used for predicting the target substance concentration of the kim-1 colloidal gold test paper; and the result generation module is used for generating an analysis result of the kim-1 colloidal gold test paper and packaging the analysis result into a structured detection report.

The invention provides a CPA-LFD method for detecting Rahnella aquatilis and a detection kit, and belongs to the technical field of microbiological detection. On the basis of a single cross primer isothermal amplification technology (CPA) and in combination with a transverse flow test strip (LFD) prepared by colloidal gold, a real-time detection method for Rahnella aquatilis under the condition of 63 DEG C isothermal amplification is developed. According to the method, a specific primer is designed, and a probe containing Biotin and an FAM label is used for identifying a DNA specific region, so that rahnella aquatica is effectively detected within 1 hour; the optimized Bst DNA polymerase can be directly applied to isothermal amplification reaction of DNA, so that the detection process is simplified, and the time is shortened; the method is simple to operate, does not need to depend on professional detection personnel, has low requirements on instruments, realizes visual interpretation of detection results, has relatively high sensitivity and specificity, can detect Rahnella aquatica with the lower limit of 48fg / mu L, is suitable for rapid detection of Rahnella aquatica in human clinical samples, animals, fishes and food pollution, and can be widely applied to detection of Rahnella aquatica. The method has important significance on monitoring, diagnosis and epidemiological prevention and control of diseases caused by rahnella aquatica.

The invention relates to a detection kit for nasopharynx cancer diagnosis and screening, which comprises an immunochromatography detection card, and the immunochromatography detection card comprises a reagent for detecting EB virus antigen and antibody through a colloidal gold labeling method. The invention also relates to application of the detection card and the EB virus antigen-antibody in preparation of a detection kit or a detection card for nasopharynx cancer diagnosis and screening.

The invention discloses a salmonella typhimurium aptamer lateral flow chromatography test strip based on the photothermal effect of a ferroferric oxide and colloidal gold composite nanomaterial, a preparation method and application, and belongs to the technical field of lateral flow chromatography test strips. The preparation of the probe comprises the step of preparing a Fe3O4 (at) Au composite nano material; the preparation method comprises the following steps: under the protection of nitrogen, dissolving FeCl3. 6H2O and FeCl2. 4H2O in deionized water, adding ammonia water, stirring at room temperature, adding trisodium citrate, collecting by using a magnet, then adding into a boiling HAuCl4 solution, keeping boiling until the color of the solution becomes reddish brown, continuing heating, cooling, and collecting by using a magnet; the test strip provided by the invention can realize quantitative detection of salmonella typhimurium.

The present invention therefore relates to a sol-gel finish layer of a non-stick coating of a household article, said layer forming a cooking surface and being free of silica and of colloidal metal oxide.

This invention provides a colloidal gold labeling method with improved sensitivity, its applications, and products, relating to the field of immunochromatography technology. The colloidal gold labeling method provided by this invention involves mixing a protein to be labeled, treated with a protein activator, with a colloidal gold solution for labeling reaction to obtain a gold-labeled protein. The protein activator includes a nonionic surfactant, a glycoprotectant, and a reducing agent. Through the synergistic effect of these components, the interactions between the gold-labeled conjugates are effectively reduced, preventing aggregation and improving the stability of the gold-labeled conjugates while protecting protein activity. The pH of the colloidal gold solution is 0.3-0.8 lower than the isoelectric point of the protein to be labeled. Under these conditions, the gold-labeled conjugates formed have good stability and high labeling efficiency. This method is simple to operate, the prepared gold-labeled conjugates have high labeling efficiency and good stability, and the prepared reagent kit has high sensitivity and is less prone to missed detections.

The utility model discloses a colloidal gold detection card collecting device, which is applied to the field of collecting devices and comprises a box body, a collecting box is arranged on one side of the box body in a penetrating manner, a buffer plate is arranged in the box body, a rubber pad attached to the collecting box is adhered to one side of the buffer plate, and the other side of the buffer plate is provided with a clamping groove. A buffer spring is connected between the other side of the buffer plate and the box body through a spring fixing piece in a bolting mode, a damper is installed between the other side of the buffer plate and the box body, a fixing frame and a fixing plate are welded to one side of the collecting box and one side of the box body correspondingly, and a moving block is arranged in the fixing frame; a limiting pin is welded to the top of the movable block. The collecting box can be limited, operation is easy, labor is saved, the sealing effect and stability of the device are improved, meanwhile, the collision buffering function can be achieved, noise generated by collision can be reduced, the influence on the hand during collision can be reduced, the service life of the device is prolonged, and practicability is high.

The application discloses a colloidal gold test strip for distinguishing between wild toxin infection and vaccine immunization of porcine pleuropneumonia actinobacillus and application. The test strip of the application takes ApxIVA truncated protein with an amino acid sequence shown as SEQ ID NO. 3 as an antigen, the truncated protein can be soluble expressed in E. coli, and the structure and function of the truncated protein are close to those of natural apxIVA. The test strip of the application comprises a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and gold-labeled chicken IgY antibody, and the C line and the T line of the NC membrane are fixed with goat anti-chicken IgY antibody and apxIVA truncated protein. The test strip only needs 25 muL of pig serum to make detection within 5-10 min, and can distinguish between APP wild strain infection and gene deletion vaccine APP-HB-04M immunized serum samples.

The present application relates to the technical field of biological medicine detection, and discloses a colloidal gold test strip for detecting anti-PLA2R antibody, a preparation method, a kit and application thereof.Capture antigens and detection antigens of the colloidal gold test strip are both PLA2R proteins, the nucleotide sequence of the PLA2R protein is shown as SEQ ID No.1, and the amino acid sequence of the PLA2R protein is shown as SEQ ID No.2.The colloidal gold test strip for detecting anti-PLA2R antibody and the kit have the characteristics of simplicity, good stability, high sensitivity, good specificity, simple operation, suitability for on-site rapid detection, rapid result output and directness, and can be applied to family and bedside detection without special laboratory and experimental personnel.

The invention belongs to the technical field of virus immunodetection, and relates to detection of swine transmissible gastroenteritis virus, in particular to a Mab 12H6 antibody, a colloidal gold test strip and application. According to the invention, TGEV N protein is used as an antigen of a detection line, Mab 12H6 is used as a detection antibody combined with colloidal gold, condition optimization is carried out through different methods, the specificity and sensitivity of the methods are evaluated, and a detection method for detecting the antigen based on an N protein TGEV colloidal gold competition method is established. The established TGEV colloidal gold test strip has the advantages of strong specificity, high sensitivity, simplicity and convenience in operation, low production cost and the like, and can be applied to rapid detection of TGEV in a porcine diarrhea sample clinically.

The invention belongs to the technical field of medical supplies, and discloses a weakly ionized colloidal gold immunochromatography test strip based on a cationic polymer as a quality control line and a preparation method thereof. The weak ionized colloidal gold immunochromatography test strip comprises a substrate, a nitrocellulose membrane, a combination pad and a water absorption pad, the nitrocellulose membrane, the combination pad and the water absorption pad are all distributed on the surface of the substrate; the combination pad is lapped on the nitrocellulose membrane, the water absorption pad is lapped on the nitrocellulose membrane, and the combination pad and the water absorption pad are distributed on two sides of the nitrocellulose membrane; a detection line and a quality control line are arranged on the nitrocellulose membrane; the quality control line is coated with poly dimethyl diallyl ammonium chloride; the weak ionized colloidal gold immunochromatography test strip further comprises a CEA monoclonal antibody Ab1-weak ionized colloidal gold compound. Directional charge adsorption synergy is formed through weak ionized colloidal gold and a PDDA quality control line with strong positive electricity, and gt is still kept in a high-salt environment; the capture stability is 95%, and the production cost of the C line is reduced by 90%.

The invention discloses a test strip for detecting Alzheimer's disease and application thereof, the test strip adopts a colloidal gold-horseradish peroxidase compound labeling technology, the detection sensitivity is remarkably improved through enzyme signal amplification, and two key AD biomarkers p-Tau-181 and A beta 1-42 in blood can be synchronously detected. The test strip comprises a sample pad, a marking pad, a nitrocellulose membrane and absorbent paper, wherein the nitrocellulose membrane is provided with two detection lines (T1 and T2) for capturing p-Tau-181 and A beta1-42 respectively and a quality control line (C). The kit is high in sensitivity, rapid in detection, simple and convenient to operate, low in cost and suitable for primary medical treatment and large-scale screening scenes, and has important value in early diagnosis, popularization, prevention and control of the Alzheimer's disease.

The application discloses a kind of based on nanobody detection swine epidemic diarrhea virus (PEDV) colloidal gold test strip preparation and application, belong to animal pathogen detection technical field.The application is based on the specific nanobody of 2 strains of anti-PEDV fiber projection glycoprotein screening as basic material, by gene engineering modification to nanobody, they are respectively with the Fc region of pig IgG and ferritin fusion expression, successfully prepared two fusion proteins.Then, with two fusion proteins as matched antibody, wherein the protein labeled with colloidal gold as detection antibody, another protein fused with ferritin is capture antibody, the colloidal gold detection test strip of PEDV is established.The test strip has the advantages of strong specificity, high sensitivity, simple operation, low production cost, etc., and can be applied to the rapid detection of PEDV in clinical pig diarrhea samples.

The utility model relates to the technical field of medical detection, in particular to a malaria colloidal gold kit capable of improving rapid detection efficiency, which comprises a kit body and a blood taking needle, the side wall of the kit body is provided with two notches, and the inner side of the kit body is fixedly provided with detection test paper; a clamping structure is mounted on the kit body and comprises a rotating shaft I rotationally connected to the side wall of the kit body. The blood taking needle is clamped and fixed through the clamping structure, so that the kit body is provided with the disposable blood taking needle in a matched mode, the disposable blood taking needle does not need to be cleaned after being used, the detection speed is greatly increased, dropped blood and buffering agents can be stirred through the stirring structure, and the detection accuracy is improved. Therefore, the mixing of the blood and the buffering agent is promoted.

The invention discloses colloidal gold immunochromatography test paper for rapidly detecting pigeon I-type paramyxovirus and a preparation method of the colloidal gold immunochromatography test paper. Viral diseases of pigeons, especially pigeon plague virus and pigeon adenovirus, are rapidly detected on the basis of a newly developed test strip, and the operation is simple and convenient; according to the design of antigens and antibodies of the pigeon plague virus and the pigeon adenovirus, rapid detection of the pigeon plague virus and the pigeon adenovirus can be conveniently carried out, and the obtained test strip is high in specificity and high in sensitivity. The pigeon I-type paramyxovirus detection kit has the advantages that the pigeon I-type paramyxovirus detection kit does not have cross reaction with chicken infectious bronchitis, chicken egg drop syndrome, chicken Marek's disease, avian influenza, fowl adenovirus, infectious bursal disease, escherichia coli, salmonella and other samples, but can have cross reaction with Newcastle disease, so that the pigeon I-type paramyxovirus detection kit can be used for diagnosing pigeon I-type paramyxovirus and Newcastle disease.

The application provides a pair of mouse monoclonal antibodies 4A5 and 2C12 with specific binding to canine rotavirus VP7 protein, and a colloidal gold detection kit prepared from the mouse monoclonal antibodies, which can detect various epidemic strains and standard strains of canine rotavirus with high sensitivity, has a high coincidence rate with an RT-PCR detection method, and can detect epidemic strains and target strains in different regions, has the advantages of rapidness, simplicity, accuracy, and can meet the urgent needs of the present clinical stage.

This invention relates to the field of diagnostic kit technology, specifically disclosing a colloidal gold immunochromatographic assay kit for Mycoplasma pneumoniae IgM antibodies, comprising a storage box, a cover, a loading unit, a detection unit, and a binding structure. The storage box is rectangular, with a pair of slots on its front side wall. One of the slots has sliding grooves on both its upper and lower side walls that communicate with the right side wall. The upper wall of the storage box, near the middle of its left end, has a first insertion port that communicates with the other slot. Through the design of the loading unit, this invention allows for sequential replacement of the internal test strips. The test strip pads are assembled in sequence, facilitating replacement after testing, reducing waste of the outer casing and environmental pollution, and also reducing usage costs. By carrying the internal test strips and a loading unit, space is saved, enabling multiple uses. Furthermore, the binding assembly allows for the binding and carrying of multiple pre-loaded test strip pads within the loading unit.

The utility model discloses a reagent kit for paraquat colloidal gold rapid detection, including box body, be provided with the placement subassembly on the box body, the placement subassembly includes eaves board and box cover, the eaves board sets up on the box body, the box cover places the top of box body, the box body inside evenly is equipped with the mounting groove, is equipped with the mounting plate on the mounting groove and is connected slidingly, the eaves board evenly is equipped with the fixed hole, is provided with the demolition subassembly between the eaves board and mounting plate, the demolition subassembly includes fixed rod and fixed cover, the fixed rod is connected on the eaves board and mounting plate slidingly, the fixed cover can detachable mounting on the fixed rod, the fixed cover one end and the box body close, is equipped with the abutment slot on the fixed cover, the fixed rod and the abutment slot place abut, to solve the technical problem that the space layout in the inside of many reagent kits in the background art does not have the technical problem of explicit separation design.

1. The name of the design product: colloidal gold immunochromatographic analyzer. 2. The use of the design product: for detecting biomolecules. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model discloses a colloidal gold test paper card for rapidly determining rice yeast acid in whole blood. The colloidal gold test paper card comprises a bottom plate as well as a glass cellulose membrane, a filtration binding area, a detection area and a water absorption pad which are sequentially arranged on the bottom plate from left to right, the filtration binding area adopts an antigen-antibody binding technology, red blood cells in whole blood can be retained on a filter membrane of a sample pad without moving, other substances in the whole blood can smoothly pass through, and the detection effect is good. According to the present invention, the kit can be combined with the rice yeast acid so as to eliminate the background interference, can be combined with the rice yeast acid, and can be detected through the detection region, such that the rice yeast acid in the whole blood can be accurately detected, the accuracy and the specificity of the rice yeast acid determination in the whole blood can be effectively improved, the application range and the efficiency of the trace whole blood sample in the detection can be expanded, and the redundant pretreatment operation is not required; the method is simple in operation and rapid in detection, and can obtain a result within 15 minutes only by taking 10 microliters of trace whole blood, and the lowest detection limit of 50 micrograms / L is reached.

A method for the extraction and enrichment of Hepatitis C Virus (HCV) antigens by conjugating specific antibodies targeting Envelope 1, Envelope 2, NS3, and NS4 antigens to a temperature-responsive smart polymer (NIPAAm-Co-HIPAAm-Co-SAKIPAAm). The application also relates to a detection kit for HCV antigens, comprising the smart polymer conjugated to specific antibodies, colloidal gold nanoparticles conjugated to specific antibodies, and instructions for detecting HCV antigens in serum, plasma, or whole blood samples.

The invention relates to the field of bioengineering, and discloses a human immunodeficiency virus p24 antigen and antibody detection kit and a preparation method thereof. The kit comprises a colloidal gold marker composite pad, wherein the colloidal gold marker composite pad comprises a p24 antibody colloidal gold marker, an HIV antigen colloidal gold marker and a chicken IgY antibody colloidal gold marker; an AG line, an AB line and a C line are arranged on the NC membrane, the AG line is coated with a p24 monoclonal antibody, the AB line is coated with HIV-I, HIV-II and HIV-O mixed antigens, and the C line is coated with goat anti-chicken IgY; and a sample pad, a conjugate pad and a water absorbent pad. The human immunodeficiency virus p24 antigen and antibody detection kit provided by the invention is obviously superior to the prior art in the aspects of sensitivity, specificity, convenience and stability, and has important clinical application and market values.

The invention relates to an antibody specifically binding to a novel coronavirus N protein or an antigen binding fragment thereof and application thereof. The antibody or the antigen binding fragment thereof has improved detection sensitivity and specificity, and the related colloidal gold test strip is convenient for rapid detection and operation, low in detection cost and wide in applicability.

The invention relates to the technical field of activity detection, and particularly discloses a colloidal gold method for rapidly detecting ACE inhibitory activity and a test paper preparation method. The test paper comprises a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorption pad, angiotensin converting enzyme labeled by colloidal gold is loaded on the gold-labeled pad, and a detection line coated with an angiotensin II antibody and a quality control line coated with an anti-ACE antibody are arranged on the nitrocellulose membrane. During detection, an ACE inhibitor in a sample can inhibit enzyme activity and influence aggregation of colloidal gold on the detection line, so that rapid judgment is realized through color comparison of the detection line and the quality control line. The invention also provides a preparation method and a detection method of the test paper, and the test paper has the advantages of simplicity and rapidness in operation, no need of large instruments, low cost, suitability for field detection and the like, and can be widely applied to drug screening, food detection and clinical sample preliminary screening.

The invention provides a monoclonal antibody of a canine distemper virus and application thereof, and belongs to the technical field of animal medicine detection. The antibody provided by the invention has ultrahigh binding affinity to the CDV H protein of the canine distemper virus, and the linear epitope is the 373-381 site of the CDV H protein. According to the kit disclosed by the invention, the nitrocellulose membrane is adopted to coat the canine virus specific antibody and the fluorescent marker, so that the virus in a canine sample can be rapidly detected. The adopted antibody is prepared through a genetic engineering means, and the detection sensitivity of the antibody is obviously superior to that of a conventional colloidal gold test strip and an ELISA detection method. The antibody disclosed by the invention keeps excellent stability in a high-temperature environment, and shows an extremely low cross reaction characteristic to pathogens such as CAV. By matching with a special fluorescence detection device, the technology can complete efficient detection of the canine distemper virus in a short time, and is suitable for clinical diagnosis and treatment and on-site rapid screening scenes.

The utility model provides a colloidal gold detection recording card which comprises a hard straight board card, a vertical sticking strip with a covering film and an information recording area, and the covering film realizes the antifouling protection of detection paper through the design of edge mucilage glue and a glue-free area. The recording card can prolong the storage life of detection paper, has strong traceability of detection data, and is suitable for medical detection, family self-test and other scenes.