222 results about "Colloidal au" patented technology

The invention discloses an intelligent colloidal gold test strip result identification and analysis method based on machine learning, particularly relates to the technical field of image detection, and is used for solving the problem of lack of analysis on a time dynamic rule of a developing process in the prior art. The method comprises the following steps: acquiring color development images of a test strip at different time points, extracting local features and global features of the color development images, and forming color development change feature vectors based on adaptive weighted fusion of feature weights; further constructing a time dynamic characteristic model of the color development reaction based on the color development change characteristic vector, and analyzing a time dynamic rule of the color development process; the method comprises the following steps: judging the stability of the morphological change of a color development region through morphological filtering and an active contour model, and calculating and evaluating the dynamic trend of a color development signal in combination with Shannon entropy; the time dynamic characteristic model is used for predicting the developing result and outputting the detection result of the test strip, so that the detection accuracy of the test strip is improved.

The invention relates to the technical field of molecular biology, in particular to an avian adenovirus serum type 4 colloidal gold test strip based on a monoclonal antibody and application of the test strip. According to the invention, two monoclonal antibodies (6B3 and 8G11) aiming at FAdV4 penton protein are successfully prepared through a hybridoma technology, and both the monoclonal antibodies show high titer and strong serotype specificity. Specific analysis proves that the two monoclonal antibodies can recognize 12 FAdV4 isolates from different sources, and do not have cross reaction with other FAdV serotypes or common poultry pathogens. According to the invention, through systematic optimization of key parameters, the detection sensitivity of the test strip is 7.81 * 10 < 4 > TCID50 / 0.1 mL, and the detection time is only 15 minutes. Clinical sample verification shows that compared with an ELISA method, the total coincidence rate is 98.6%.

The invention belongs to the technical field of colloidal gold immunochromatography, and relates to a cetirizine monoclonal antibody, a nucleic acid molecule, a carrier, a detection kit and application thereof. The cetirizine monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises a VH-CDR1, a VH-CDR2 and a VH-CDR3, the light chain variable region comprises a VL-CDR1, a VL-CDR2 and a VL-CDR3, the sequence of the VH-CDR1 is as shown in SEQ ID NO.1, the sequence of the VH-CDR2 is as shown in SEQ ID NO.2, and the sequence of the VH-CDR3 is as shown in SEQ ID NO.3. The invention further discloses a preparation method of the cetirizine monoclonal antibody. The monoclonal antibody provided by the invention has high sensitivity to cetirizine, the lowest detection limit is 0.007 ng / mL, the quantitative range is 0.017-0.282 ng / mL, and the specificity is strong.

The invention relates to the technical field of medical biology, in particular to a method for identifying and diagnosing PEDV (Porcine Epidemic Diarrhea Virus) and PoRV-A. Colloidal gold is used as a carrier, and a monoclonal antibody (mAb) for resisting PEDV and PoRV-A and an initiator are respectively modified on colloidal gold (Au) to prepare a probe (Au-I-mAb). An ELISA hole is coated with a monoclonal antibody (mAb), when a target virus exists, a probe is added to form a double-antibody sandwich compound (mAb-antibody-mAb-Au-I), a hairpin is added, and an initiation chain on the compound recognizes the matched hairpin and induces the hairpin to cross to generate HCR. Due to the fact that the hairpins are marked by different fluorophores, when H1 is in a closed hairpin structure, fluorescence on H1 is quenched by a quenching group, after H1 is subjected to ring opening through HCR, the fluorophores are away from the quenching group, and fluorescence is recovered. Fluorescence excitation is carried out by using corresponding emission wavelengths, and two target pathogens are finally detected at the same time by detecting output signals of different fluorophores.

The invention provides a primer group and a kit for detecting bovine mycobacterium paratuberculosis based on a recombinase isothermal amplification technology and application, and relates to the technical field of biological detection.The primer group comprises a forward primer MAP-F3, a reverse primer MAP-R3 and a specific fluorescent probe MAP-P3 and has good specificity; the sensitivity reaches 10fg / mu L, which is more sensitive than that of a colloidal gold test strip, the dependence on equipment is broken through, and the detection result is more accurate. The technical problem that in the prior art, it is difficult to distinguish bovine mycobacterium paratuberculosis from other pathogens through a detection method, or the detection process is tedious is solved. According to the prepared reagent or kit, the liquid preparation process is simple, the whole amplification process can be completed only in 30 min, the amplification yield can reach 109-1010 copies, the method is rapid and efficient, the amplification result can be directly judged according to fluorescence data, other auxiliary means are not needed, and the method is suitable for field detection.

The invention relates to a clostridium difficile triple detection kit based on colloidal gold immunochromatography and a preparation method of the clostridium difficile triple detection kit, and belongs to the technical field of immunodetection. The clostridium difficile triple detection kit provided by the invention comprises a clostridium difficile triple detection card and a sample diluent, and the clostridium difficile triple detection card is formed by arranging and connecting three detection test strips in parallel; the detection test strip comprises a clostridium difficile toxin A detection test strip, a clostridium difficile toxin B detection test strip and a clostridium difficile glutamate dehydrogenase detection test strip. By using the clostridium difficile triple detection kit provided by the invention, three markers related to clostridium difficile can be detected at the same time, the detection limit of clostridium difficile toxin A is 0.25 ng / mL, the detection limit of clostridium difficile toxin B is 0.5 ng / mL, and the detection limit of clostridium difficile glutamate dehydrogenase is 0.5 ng / mL, so that the clostridium difficile triple detection kit has the advantages of good sensitivity, simplicity in operation, high sensitivity, high sensitivity, high sensitivity and the like. The method is suitable for large-scale popularization screening.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

The invention relates to a colloidal gold test strip for detecting a sheep pox virus antibody as well as a preparation method and application of the colloidal gold test strip. According to the invention, firstly, a recombinant 122 protein is prepared, and the recombinant 122 protein has remarkable antigenicity and immunogenicity and can be used as a good target for diagnosing the sheep pox virus; secondly, the colloidal gold test strip for detecting the sheep pox virus antibody is prepared, a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorption pad are sequentially arranged on a PVC base plate of the test strip, and the gold-labeled pad is coated with gold-labeled recombinant 122 protein; the test strip can simultaneously detect antibodies of the sheep pox virus, the bovine nodular skin disease virus and the goat pox virus, the sensitivity can reach 1: 128, 1: 128 and 1: 64 respectively, the test strip has no cross reaction with bovine viral diarrhea virus, foot-and-mouth disease O-type virus, foot-and-mouth disease A-type virus, orf virus, brucellosis and colibacillosis, the specificity is good, and the diagnosis coincidence rate reaches 93%.

The invention discloses a preparation method and application of a colloidal gold immunochromatography test strip for detecting IDH1 mutant protein, and belongs to the field of rapid immunodetection. The colloidal gold chromatography test strip comprises a bottom plate, a sample pad, a nitrocellulose membrane, a water absorption pad and colloidal gold, the nitrocellulose membrane is sequentially provided with a detection line T and a quality control line C in the flowing direction of a sample, the detection line T is coated with IDH1 mutant protein, and the quality control line C is coated with a second antibody corresponding to a monoclonal antibody of the IDH1 mutant protein. Clinical tumor tissue samples collected in real time in glioma resection surgery are treated, an object to be detected and an immune gold label are mixed, incubated and dropped in a test strip sample adding hole, and the object to be detected runs along the test strip under the driving of a buffer solution and generates visual color change. The test strip has the advantages of being rapid in reaction, easy and convenient to operate, convenient to carry, high in specificity and the like, makes up the blank of an IDH1 mutant protein concentration quantitative method, and has good clinical application value.

The invention provides a colloidal gold test strip for detecting renal injury molecule 1 (kim-1) and a preparation method thereof, and aims to provide a bedside rapid diagnosis tool which is high in accuracy, strong in specificity, simple to operate and capable of rapidly reporting results. The test strip is formed by sequentially connecting a plastic bottom lining, a nitrocellulose membrane, a gold label pad, a sample pad and a water absorption pad, wherein a detection line and a quality control line are arranged on the nitrocellulose membrane. The detection line contains a KIM-1 monoclonal antibody, the quality control line contains a mouse anti-human IgG antibody, and the colloidal gold pad is coated with a colloidal gold labeled KIM-1 monoclonal antibody, so that a double-antibody sandwich structure is formed to specifically recognize human KIM-1. The preparation method comprises the following steps: spraying an antibody on the nitrocellulose membrane to form the detection line and the quality control line, preparing a colloidal gold antibody compound solution, and assembling each component. By optimizing the antibody concentration and a colloidal gold preparation process, the sensitivity and the stability of the test strip are ensured. The test strip is suitable for rapidly detecting kidney injury, and has important value for early diagnosis and treatment monitoring of acute and chronic kidney injury.

The invention belongs to the technical field of biological detection, and particularly relates to a preparation method of lipopolysaccharide-beta-1, 3-glucan binding protein and application of the lipopolysaccharide-beta-1, 3-glucan binding protein in preparation of a broad-spectrum colloidal gold detection product for dermatophytes. The prokaryotic expression system induces the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein to be efficiently expressed in an inclusion body form, the optimized inclusion body purification-renaturation process is combined to realize batch preparation, and the colloidal gold chromatography technology is integrated, so that the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein is obtained. The bottlenecks of the traditional detection method in sensitivity, broad spectrum and field applicability are broken through.

The invention discloses a deep learning-based colloidal gold test paper result analysis system for detecting renal injury molecular 1. The system comprises an image acquisition module, a region processing module, an effectiveness judgment module, a concentration analysis module and a result generation module, the image acquisition module is used for acquiring a visible light image of the kim-1 colloidal gold test paper, wherein the visible light image at least comprises a detection line area and a quality control line area, and carrying out gray standardization processing, contrast enhancement processing, edge sharpening processing and target area positioning processing on the visible light image; the region processing module is used for performing positioning and boundary segmentation to obtain a mask image which is consistent with the input image in size and is marked with the position of each functional region; the effectiveness judgment module is used for judging whether the kim-1 colloidal gold test paper is effective or not; the concentration analysis module is used for predicting the target substance concentration of the kim-1 colloidal gold test paper; and the result generation module is used for generating an analysis result of the kim-1 colloidal gold test paper and packaging the analysis result into a structured detection report.

The utility model provides a colloidal gold reagent strip for detecting aminoglycoside antibiotics, which comprises a shell and a colloidal gold reagent strip, and the upper opening of the shell is connected with an upper cover in a sliding manner; a detection chamber is formed in the shell, the interior of the detection chamber is rotatably connected with a driving shaft and a driven shaft, and one end part of the driving shaft penetrates through the inner wall of the detection chamber, extends to the exterior of the shell and forms a screwing part; the surface of the driving shaft is sleeved with one end of a transmission belt, the surface of the driven shaft is sleeved with the other end of the transmission belt, a plurality of colloidal gold reagent strips are pasted on the surface of the transmission belt, and sample adding grooves, observation grooves and type display grooves are formed in the positions, corresponding to the colloidal gold reagent strips, of the top of the upper cover; according to the utility model, the types of required colloidal gold reagent strips can be selected according to different samples, the required reagent strips can be selected for multiple times and the samples can be continuously dropped through the sample adding groove when multiple experiments are carried out, the results of a plurality of reagent strips can appear at the same time, and the experiment efficiency is improved.

The invention discloses a rapid detection kit for protein residues. The rapid detection kit comprises a labeled antibody, a coated antibody and a colloidal gold test strip. The labeled antibody is prepared by treating human intestinal epithelial cells and then immunizing a BALB / c mouse through a series of steps; and the coated antibody is prepared by treating human intestinal epithelial cells, immunizing New Zealand white rabbits and purifying in a subsequent process. The colloidal gold test strip is prepared into colloidal gold through a specific method, the labeled antibody and the colloidal gold are incubated, sprayed and dried, a sample pad is processed and then dried, impression and drying are conducted on a PVC bottom plate pasted with an NC film, and then cutting and assembling are conducted. The kit is high in detection speed and meets the instrument turnover requirement; the accuracy is high, and misjudgment is reduced; the method has the advantages that the sensitivity is as low as 100ng / mL, trace protein residues can be accurately detected, the risks of endoscopic infection and the like are reduced, and the method has obvious advantages in the aspect of protein residue detection of medical instruments and has a great application value.

The invention provides a CPA-LFD method for detecting Rahnella aquatilis and a detection kit, and belongs to the technical field of microbiological detection. On the basis of a single cross primer isothermal amplification technology (CPA) and in combination with a transverse flow test strip (LFD) prepared by colloidal gold, a real-time detection method for Rahnella aquatilis under the condition of 63 DEG C isothermal amplification is developed. According to the method, a specific primer is designed, and a probe containing Biotin and an FAM label is used for identifying a DNA specific region, so that rahnella aquatica is effectively detected within 1 hour; the optimized Bst DNA polymerase can be directly applied to isothermal amplification reaction of DNA, so that the detection process is simplified, and the time is shortened; the method is simple to operate, does not need to depend on professional detection personnel, has low requirements on instruments, realizes visual interpretation of detection results, has relatively high sensitivity and specificity, can detect Rahnella aquatica with the lower limit of 48fg / mu L, is suitable for rapid detection of Rahnella aquatica in human clinical samples, animals, fishes and food pollution, and can be widely applied to detection of Rahnella aquatica. The method has important significance on monitoring, diagnosis and epidemiological prevention and control of diseases caused by rahnella aquatica.

The invention relates to a detection kit for nasopharynx cancer diagnosis and screening, which comprises an immunochromatography detection card, and the immunochromatography detection card comprises a reagent for detecting EB virus antigen and antibody through a colloidal gold labeling method. The invention also relates to application of the detection card and the EB virus antigen-antibody in preparation of a detection kit or a detection card for nasopharynx cancer diagnosis and screening.

The invention discloses a salmonella typhimurium aptamer lateral flow chromatography test strip based on the photothermal effect of a ferroferric oxide and colloidal gold composite nanomaterial, a preparation method and application, and belongs to the technical field of lateral flow chromatography test strips. The preparation of the probe comprises the step of preparing a Fe3O4 (at) Au composite nano material; the preparation method comprises the following steps: under the protection of nitrogen, dissolving FeCl3. 6H2O and FeCl2. 4H2O in deionized water, adding ammonia water, stirring at room temperature, adding trisodium citrate, collecting by using a magnet, then adding into a boiling HAuCl4 solution, keeping boiling until the color of the solution becomes reddish brown, continuing heating, cooling, and collecting by using a magnet; the test strip provided by the invention can realize quantitative detection of salmonella typhimurium.

The present invention therefore relates to a sol-gel finish layer of a non-stick coating of a household article, said layer forming a cooking surface and being free of silica and of colloidal metal oxide.

This invention provides a colloidal gold labeling method with improved sensitivity, its applications, and products, relating to the field of immunochromatography technology. The colloidal gold labeling method provided by this invention involves mixing a protein to be labeled, treated with a protein activator, with a colloidal gold solution for labeling reaction to obtain a gold-labeled protein. The protein activator includes a nonionic surfactant, a glycoprotectant, and a reducing agent. Through the synergistic effect of these components, the interactions between the gold-labeled conjugates are effectively reduced, preventing aggregation and improving the stability of the gold-labeled conjugates while protecting protein activity. The pH of the colloidal gold solution is 0.3-0.8 lower than the isoelectric point of the protein to be labeled. Under these conditions, the gold-labeled conjugates formed have good stability and high labeling efficiency. This method is simple to operate, the prepared gold-labeled conjugates have high labeling efficiency and good stability, and the prepared reagent kit has high sensitivity and is less prone to missed detections.

The utility model discloses a colloidal gold detection card collecting device, which is applied to the field of collecting devices and comprises a box body, a collecting box is arranged on one side of the box body in a penetrating manner, a buffer plate is arranged in the box body, a rubber pad attached to the collecting box is adhered to one side of the buffer plate, and the other side of the buffer plate is provided with a clamping groove. A buffer spring is connected between the other side of the buffer plate and the box body through a spring fixing piece in a bolting mode, a damper is installed between the other side of the buffer plate and the box body, a fixing frame and a fixing plate are welded to one side of the collecting box and one side of the box body correspondingly, and a moving block is arranged in the fixing frame; a limiting pin is welded to the top of the movable block. The collecting box can be limited, operation is easy, labor is saved, the sealing effect and stability of the device are improved, meanwhile, the collision buffering function can be achieved, noise generated by collision can be reduced, the influence on the hand during collision can be reduced, the service life of the device is prolonged, and practicability is high.

The application discloses a colloidal gold test strip for distinguishing between wild toxin infection and vaccine immunization of porcine pleuropneumonia actinobacillus and application. The test strip of the application takes ApxIVA truncated protein with an amino acid sequence shown as SEQ ID NO. 3 as an antigen, the truncated protein can be soluble expressed in E. coli, and the structure and function of the truncated protein are close to those of natural apxIVA. The test strip of the application comprises a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and gold-labeled chicken IgY antibody, and the C line and the T line of the NC membrane are fixed with goat anti-chicken IgY antibody and apxIVA truncated protein. The test strip only needs 25 muL of pig serum to make detection within 5-10 min, and can distinguish between APP wild strain infection and gene deletion vaccine APP-HB-04M immunized serum samples.

The invention discloses a method for detecting isofenphos-methyl in vegetables and fruits based on colloidal gold immunochromatography, and relates to the technical field of pesticide residue detection.The method for detecting isofenphos-methyl in vegetables and fruits comprises the following steps that a colloidal gold immunochromatography reagent strip is prepared; acquiring a color developing image of the colloidal gold immunochromatography reagent strip; obtaining the color of the detection line and the color of the control line; obtaining color feature data of the detection line and color feature data of the control line; the concentration of the isofenphos-methyl in the vegetables and fruits is obtained. The colloidal gold immunochromatography reagent strip is used for detecting isofenphos-methyl, the whole detection process can be completed in a short time by combining color development image processing, and the colloidal gold immunochromatography method is easy to operate and low in cost, so that the colloidal gold immunochromatography reagent strip is convenient to popularize and apply in primary laboratories and even field rapid detection.

The invention discloses a self-adaptive ovulation test paper detection method which comprises the following steps: flatly placing colloidal gold ovulation test paper soaked with urine into a test paper groove of a test paper photographing device, fixing a mobile phone on a mobile phone clamping table of the test paper photographing device, and enabling a mobile phone camera to face the test paper; by adjusting the height of a mobile phone clamping table and the position of a mobile phone of the test paper photographing device, a camera of the mobile phone can shoot a clear and upright test paper picture; analyzing the clear and upright test paper picture to determine the model of the test paper; querying a test paper concentration algorithm database in which a plurality of test paper concentration algorithms are stored according to the determined test paper model to obtain a test paper concentration algorithm corresponding to the test paper model; using the obtained test paper concentration algorithm to process the clear and upright test paper photo to obtain the LH concentration value of the test paper; and estimating the ovulation time according to the LH concentration value of the test paper.

The present application relates to the technical field of biological medicine detection, and discloses a colloidal gold test strip for detecting anti-PLA2R antibody, a preparation method, a kit and application thereof.Capture antigens and detection antigens of the colloidal gold test strip are both PLA2R proteins, the nucleotide sequence of the PLA2R protein is shown as SEQ ID No.1, and the amino acid sequence of the PLA2R protein is shown as SEQ ID No.2.The colloidal gold test strip for detecting anti-PLA2R antibody and the kit have the characteristics of simplicity, good stability, high sensitivity, good specificity, simple operation, suitability for on-site rapid detection, rapid result output and directness, and can be applied to family and bedside detection without special laboratory and experimental personnel.

According to the multiple mycotoxin target detection method based on CRISPR-Cas12a provided by the invention, multiple detection of OTA and AFB1 is successfully realized by utilizing a transverse flow analysis platform assisted by CRISPR / Cas12a under the condition that Cas12a and crRNA are not replaced, and the experiment cost is reduced. Compared with a commercial colloidal gold test strip, the detection sensitivity is improved by 1-2 orders of magnitude. Under the condition that a complex magnetic separation step is not needed, a Signal-on signal mode can be directly achieved, and complex experiment operation is reduced. The research shows the potential of the MC-LFA strategy for food safety detection in a resource limited environment, and the detection target range can be effectively expanded only by replacing the aptamer sequence, so that the MC-LFA strategy becomes a general technology suitable for various analysis schemes, and has potential application prospects in multiple fields such as public health, agriculture and environment monitoring.

The invention belongs to the technical field of virus immunodetection, and relates to detection of swine transmissible gastroenteritis virus, in particular to a Mab 12H6 antibody, a colloidal gold test strip and application. According to the invention, TGEV N protein is used as an antigen of a detection line, Mab 12H6 is used as a detection antibody combined with colloidal gold, condition optimization is carried out through different methods, the specificity and sensitivity of the methods are evaluated, and a detection method for detecting the antigen based on an N protein TGEV colloidal gold competition method is established. The established TGEV colloidal gold test strip has the advantages of strong specificity, high sensitivity, simplicity and convenience in operation, low production cost and the like, and can be applied to rapid detection of TGEV in a porcine diarrhea sample clinically.

The invention relates to the technical field of detection reagents, in particular to a test strip for detecting mites, application of the test strip and a kit for detecting the mites. The test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and absorbent paper, the combination pad is coated with an anti-mite antibody marked by colloidal gold; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with a mite antigen, and the quality control line is coated with a goat anti-rabbit IgG antibody. The test strip is prepared by using a colloidal gold method, and the adopted principle is a double-antibody sandwich method. By utilizing the test strip disclosed by the invention, whether the acarid is contained or not can be obtained within 5-10 minutes without an extra detection instrument, and the detection method is high in accuracy. The possibility is provided for efficiently and quickly detecting the mites.

1. Name of the Design Product: Detection Card (Colloidal Gold Quadruple Card). 2. Use of the Design Product: The design product is a detection card for biological detection. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.

The invention provides a rapid detection method for detecting a rice stripe virus in a laodelphax striatellus mixed sample based on a colloidal gold immune test strip. The method is used for detecting whether laodelphax striatellus carrying RSV exists in a 3-5-age laodelphax striatellus nymph or adult population, and only one laodelphax striatellus carrying RSV within 2-50 laodelphax striatellus can be detected. The detection period is shortened to 30-60 minutes, the detection method is simple, convenient and rapid, low in cost and suitable for field real-time detection, and necessary technical guarantee is provided for establishment of a precise early warning and monitoring system for the rice stripe disease.

The invention belongs to the technical field of medical supplies, and discloses a weakly ionized colloidal gold immunochromatography test strip based on a cationic polymer as a quality control line and a preparation method thereof. The weak ionized colloidal gold immunochromatography test strip comprises a substrate, a nitrocellulose membrane, a combination pad and a water absorption pad, the nitrocellulose membrane, the combination pad and the water absorption pad are all distributed on the surface of the substrate; the combination pad is lapped on the nitrocellulose membrane, the water absorption pad is lapped on the nitrocellulose membrane, and the combination pad and the water absorption pad are distributed on two sides of the nitrocellulose membrane; a detection line and a quality control line are arranged on the nitrocellulose membrane; the quality control line is coated with poly dimethyl diallyl ammonium chloride; the weak ionized colloidal gold immunochromatography test strip further comprises a CEA monoclonal antibody Ab1-weak ionized colloidal gold compound. Directional charge adsorption synergy is formed through weak ionized colloidal gold and a PDDA quality control line with strong positive electricity, and gt is still kept in a high-salt environment; the capture stability is 95%, and the production cost of the C line is reduced by 90%.