77 results about "Colloidal au" patented technology

The invention relates to the technical field of molecular biology, in particular to an avian adenovirus serum type 4 colloidal gold test strip based on a monoclonal antibody and application of the test strip. According to the invention, two monoclonal antibodies (6B3 and 8G11) aiming at FAdV4 penton protein are successfully prepared through a hybridoma technology, and both the monoclonal antibodies show high titer and strong serotype specificity. Specific analysis proves that the two monoclonal antibodies can recognize 12 FAdV4 isolates from different sources, and do not have cross reaction with other FAdV serotypes or common poultry pathogens. According to the invention, through systematic optimization of key parameters, the detection sensitivity of the test strip is 7.81 * 10 < 4 > TCID50 / 0.1 mL, and the detection time is only 15 minutes. Clinical sample verification shows that compared with an ELISA method, the total coincidence rate is 98.6%.

The invention belongs to the technical field of colloidal gold immunochromatography, and relates to a cetirizine monoclonal antibody, a nucleic acid molecule, a carrier, a detection kit and application thereof. The cetirizine monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises a VH-CDR1, a VH-CDR2 and a VH-CDR3, the light chain variable region comprises a VL-CDR1, a VL-CDR2 and a VL-CDR3, the sequence of the VH-CDR1 is as shown in SEQ ID NO.1, the sequence of the VH-CDR2 is as shown in SEQ ID NO.2, and the sequence of the VH-CDR3 is as shown in SEQ ID NO.3. The invention further discloses a preparation method of the cetirizine monoclonal antibody. The monoclonal antibody provided by the invention has high sensitivity to cetirizine, the lowest detection limit is 0.007 ng / mL, the quantitative range is 0.017-0.282 ng / mL, and the specificity is strong.

The invention belongs to the technical field of biological detection, and particularly relates to a preparation method of lipopolysaccharide-beta-1, 3-glucan binding protein and application of the lipopolysaccharide-beta-1, 3-glucan binding protein in preparation of a broad-spectrum colloidal gold detection product for dermatophytes. The prokaryotic expression system induces the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein to be efficiently expressed in an inclusion body form, the optimized inclusion body purification-renaturation process is combined to realize batch preparation, and the colloidal gold chromatography technology is integrated, so that the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein is obtained. The bottlenecks of the traditional detection method in sensitivity, broad spectrum and field applicability are broken through.

The invention discloses a deep learning-based colloidal gold test paper result analysis system for detecting renal injury molecular 1. The system comprises an image acquisition module, a region processing module, an effectiveness judgment module, a concentration analysis module and a result generation module, the image acquisition module is used for acquiring a visible light image of the kim-1 colloidal gold test paper, wherein the visible light image at least comprises a detection line area and a quality control line area, and carrying out gray standardization processing, contrast enhancement processing, edge sharpening processing and target area positioning processing on the visible light image; the region processing module is used for performing positioning and boundary segmentation to obtain a mask image which is consistent with the input image in size and is marked with the position of each functional region; the effectiveness judgment module is used for judging whether the kim-1 colloidal gold test paper is effective or not; the concentration analysis module is used for predicting the target substance concentration of the kim-1 colloidal gold test paper; and the result generation module is used for generating an analysis result of the kim-1 colloidal gold test paper and packaging the analysis result into a structured detection report.

The invention relates to a detection kit for nasopharynx cancer diagnosis and screening, which comprises an immunochromatography detection card, and the immunochromatography detection card comprises a reagent for detecting EB virus antigen and antibody through a colloidal gold labeling method. The invention also relates to application of the detection card and the EB virus antigen-antibody in preparation of a detection kit or a detection card for nasopharynx cancer diagnosis and screening.

The present invention therefore relates to a sol-gel finish layer of a non-stick coating of a household article, said layer forming a cooking surface and being free of silica and of colloidal metal oxide.

This invention provides a colloidal gold labeling method with improved sensitivity, its applications, and products, relating to the field of immunochromatography technology. The colloidal gold labeling method provided by this invention involves mixing a protein to be labeled, treated with a protein activator, with a colloidal gold solution for labeling reaction to obtain a gold-labeled protein. The protein activator includes a nonionic surfactant, a glycoprotectant, and a reducing agent. Through the synergistic effect of these components, the interactions between the gold-labeled conjugates are effectively reduced, preventing aggregation and improving the stability of the gold-labeled conjugates while protecting protein activity. The pH of the colloidal gold solution is 0.3-0.8 lower than the isoelectric point of the protein to be labeled. Under these conditions, the gold-labeled conjugates formed have good stability and high labeling efficiency. This method is simple to operate, the prepared gold-labeled conjugates have high labeling efficiency and good stability, and the prepared reagent kit has high sensitivity and is less prone to missed detections.

The present application relates to the technical field of biological medicine detection, and discloses a colloidal gold test strip for detecting anti-PLA2R antibody, a preparation method, a kit and application thereof.Capture antigens and detection antigens of the colloidal gold test strip are both PLA2R proteins, the nucleotide sequence of the PLA2R protein is shown as SEQ ID No.1, and the amino acid sequence of the PLA2R protein is shown as SEQ ID No.2.The colloidal gold test strip for detecting anti-PLA2R antibody and the kit have the characteristics of simplicity, good stability, high sensitivity, good specificity, simple operation, suitability for on-site rapid detection, rapid result output and directness, and can be applied to family and bedside detection without special laboratory and experimental personnel.

The invention discloses a test strip for detecting Alzheimer's disease and application thereof, the test strip adopts a colloidal gold-horseradish peroxidase compound labeling technology, the detection sensitivity is remarkably improved through enzyme signal amplification, and two key AD biomarkers p-Tau-181 and A beta 1-42 in blood can be synchronously detected. The test strip comprises a sample pad, a marking pad, a nitrocellulose membrane and absorbent paper, wherein the nitrocellulose membrane is provided with two detection lines (T1 and T2) for capturing p-Tau-181 and A beta1-42 respectively and a quality control line (C). The kit is high in sensitivity, rapid in detection, simple and convenient to operate, low in cost and suitable for primary medical treatment and large-scale screening scenes, and has important value in early diagnosis, popularization, prevention and control of the Alzheimer's disease.

The utility model relates to the technical field of medical detection, in particular to a malaria colloidal gold kit capable of improving rapid detection efficiency, which comprises a kit body and a blood taking needle, the side wall of the kit body is provided with two notches, and the inner side of the kit body is fixedly provided with detection test paper; a clamping structure is mounted on the kit body and comprises a rotating shaft I rotationally connected to the side wall of the kit body. The blood taking needle is clamped and fixed through the clamping structure, so that the kit body is provided with the disposable blood taking needle in a matched mode, the disposable blood taking needle does not need to be cleaned after being used, the detection speed is greatly increased, dropped blood and buffering agents can be stirred through the stirring structure, and the detection accuracy is improved. Therefore, the mixing of the blood and the buffering agent is promoted.

The application provides a pair of mouse monoclonal antibodies 4A5 and 2C12 with specific binding to canine rotavirus VP7 protein, and a colloidal gold detection kit prepared from the mouse monoclonal antibodies, which can detect various epidemic strains and standard strains of canine rotavirus with high sensitivity, has a high coincidence rate with an RT-PCR detection method, and can detect epidemic strains and target strains in different regions, has the advantages of rapidness, simplicity, accuracy, and can meet the urgent needs of the present clinical stage.

The utility model discloses a reagent kit for paraquat colloidal gold rapid detection, including box body, be provided with the placement subassembly on the box body, the placement subassembly includes eaves board and box cover, the eaves board sets up on the box body, the box cover places the top of box body, the box body inside evenly is equipped with the mounting groove, is equipped with the mounting plate on the mounting groove and is connected slidingly, the eaves board evenly is equipped with the fixed hole, is provided with the demolition subassembly between the eaves board and mounting plate, the demolition subassembly includes fixed rod and fixed cover, the fixed rod is connected on the eaves board and mounting plate slidingly, the fixed cover can detachable mounting on the fixed rod, the fixed cover one end and the box body close, is equipped with the abutment slot on the fixed cover, the fixed rod and the abutment slot place abut, to solve the technical problem that the space layout in the inside of many reagent kits in the background art does not have the technical problem of explicit separation design.

1. The name of the design product: colloidal gold immunochromatographic analyzer. 2. The use of the design product: for detecting biomolecules. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model discloses a colloidal gold test paper card for rapidly determining rice yeast acid in whole blood. The colloidal gold test paper card comprises a bottom plate as well as a glass cellulose membrane, a filtration binding area, a detection area and a water absorption pad which are sequentially arranged on the bottom plate from left to right, the filtration binding area adopts an antigen-antibody binding technology, red blood cells in whole blood can be retained on a filter membrane of a sample pad without moving, other substances in the whole blood can smoothly pass through, and the detection effect is good. According to the present invention, the kit can be combined with the rice yeast acid so as to eliminate the background interference, can be combined with the rice yeast acid, and can be detected through the detection region, such that the rice yeast acid in the whole blood can be accurately detected, the accuracy and the specificity of the rice yeast acid determination in the whole blood can be effectively improved, the application range and the efficiency of the trace whole blood sample in the detection can be expanded, and the redundant pretreatment operation is not required; the method is simple in operation and rapid in detection, and can obtain a result within 15 minutes only by taking 10 microliters of trace whole blood, and the lowest detection limit of 50 micrograms / L is reached.

A method for the extraction and enrichment of Hepatitis C Virus (HCV) antigens by conjugating specific antibodies targeting Envelope 1, Envelope 2, NS3, and NS4 antigens to a temperature-responsive smart polymer (NIPAAm-Co-HIPAAm-Co-SAKIPAAm). The application also relates to a detection kit for HCV antigens, comprising the smart polymer conjugated to specific antibodies, colloidal gold nanoparticles conjugated to specific antibodies, and instructions for detecting HCV antigens in serum, plasma, or whole blood samples.

The invention relates to the field of bioengineering, and discloses a human immunodeficiency virus p24 antigen and antibody detection kit and a preparation method thereof. The kit comprises a colloidal gold marker composite pad, wherein the colloidal gold marker composite pad comprises a p24 antibody colloidal gold marker, an HIV antigen colloidal gold marker and a chicken IgY antibody colloidal gold marker; an AG line, an AB line and a C line are arranged on the NC membrane, the AG line is coated with a p24 monoclonal antibody, the AB line is coated with HIV-I, HIV-II and HIV-O mixed antigens, and the C line is coated with goat anti-chicken IgY; and a sample pad, a conjugate pad and a water absorbent pad. The human immunodeficiency virus p24 antigen and antibody detection kit provided by the invention is obviously superior to the prior art in the aspects of sensitivity, specificity, convenience and stability, and has important clinical application and market values.

The invention relates to the technical field of activity detection, and particularly discloses a colloidal gold method for rapidly detecting ACE inhibitory activity and a test paper preparation method. The test paper comprises a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorption pad, angiotensin converting enzyme labeled by colloidal gold is loaded on the gold-labeled pad, and a detection line coated with an angiotensin II antibody and a quality control line coated with an anti-ACE antibody are arranged on the nitrocellulose membrane. During detection, an ACE inhibitor in a sample can inhibit enzyme activity and influence aggregation of colloidal gold on the detection line, so that rapid judgment is realized through color comparison of the detection line and the quality control line. The invention also provides a preparation method and a detection method of the test paper, and the test paper has the advantages of simplicity and rapidness in operation, no need of large instruments, low cost, suitability for field detection and the like, and can be widely applied to drug screening, food detection and clinical sample preliminary screening.

The utility model provides a colloidal gold detection recording card which comprises a hard straight board card, a vertical sticking strip with a covering film and an information recording area, and the covering film realizes the antifouling protection of detection paper through the design of edge mucilage glue and a glue-free area. The recording card can prolong the storage life of detection paper, has strong traceability of detection data, and is suitable for medical detection, family self-test and other scenes.

The application discloses a fluorescent lateral flow immunoassay method and preparation and application of a test strip thereof, and the AuNCs-LFA test strip comprises a sample pad, a gold label pad, an NC membrane, an absorption pad and a PVC bottom plate; wherein the NC membrane comprises a signal probe AuNCs-Ab, an antigen ZEN-BSA and a goat anti-mouse lgG. The application provides a fluorescent LFA detection method based on metal nanocluster-labeled antibodies, which can be used for detecting ZEN and other small molecule compounds. Compared with a traditional colloidal gold LFA method, the method has improved speed and sensitivity for detecting small molecules.

The invention discloses colloidal gold chromatography test paper and a kit for detecting platelet CD36 antigen, the colloidal gold chromatography test paper comprises a bottom plate, a sample pad, a gold-labeled combination pad, a nitrocellulose membrane and a water absorption pad, the gold-labeled combination pad is coated with a colloidal gold labeled mouse anti-human CD36 IgG2a monoclonal antibody and a colloidal gold labeled quality control rabbit IgG polyclonal antibody; the nitrocellulose membrane is provided with a detection line coated with a human platelet CD36 antigen capture antibody and a quality control line coated with quality control goat anti-rabbit polyclonal antibody molecules. When the kit is used for detecting the CD36 antigen, a detected sample is fully split by the platelet lysis buffer and then is added into the sample adding hole of the kit, and a detection result can be observed after waiting for 10-15 minutes. By adopting the detection mode provided by the invention, the CD36 antigen on the human platelets can be quickly, simply, conveniently, sensitively and specifically detected.

The invention relates to an antigen construct for rapid immune detection, in particular to an antigen molecule for realizing dimerization or trimerization through foldon or a variant thereof. The antigen construct can enhance the conformation stability and improve the antibody binding efficiency, so that the sensitivity and shelf life stability in colloidal gold, fluorescent microspheres, ELISA (enzyme-linked immuno sorbent assay) and other in-vitro diagnosis methods in immunochromatography are remarkably improved. The construct is suitable for antibody detection of rabies, influenza, coronavirus, Brucella, tuberculosis and the like in zoonosis and HIV, HBV, HCV, syphilis and the like in human infectious diseases, and can be widely applied to clinical screening, animal quarantine, family self-test and public health emergency monitoring.

The invention relates to the technical field of biological detection, in particular to a PYD urine colloidal gold diagnostic kit which comprises a detection unit and a butt joint unit, the detection unit comprises a detection plate, detection paper, a detection groove, a sampler and a tube head, the detection paper is arranged in the detection plate, the detection groove is formed in the upper surface of the detection plate, and the sampler is arranged in the detection plate. The butt-joint unit comprises a butt-joint ring fixedly connected to the upper surface of the detection plate, the middle of the butt-joint ring is fixedly connected with a butt-joint plate, and a butt-joint groove is formed in the upper surface of the butt-joint ring in a penetrating mode. Compared with the prior art, the butt joint of the sampler and the butt joint ring is achieved by arranging the butt joint unit, the pipe head can smoothly slide into the butt joint groove by aligning the point location rod to the positioning hole and pushing the sampler downwards, the lantern ring is driven by the pressing rod to rotate to drive the sealing plate to be opened, and butt joint is achieved; the condition of pollution caused by leakage in the lofting process can be avoided while lofting of the sampler is facilitated, and the cleanliness of the device and the environment is ensured.

1. Name of the product in this design: Packaging Box (Colloidal Gold). 2. Purpose of this design: For packaging reagents. 3. The key design feature of this product is its pattern. 4. The image or photograph that best illustrates the design's key features: the front view.

The invention discloses a colloidal gold immunochromatography test strip for saliva sample detection and a preparation method thereof.The test strip comprises a supporting layer, and a sample pad, a combination pad, an NC membrane and a water absorption layer are sequentially arranged on the supporting layer from the sample introduction end; at least partial areas of the sample pad and the combination pad are obtained by drying after being pretreated by saliva sample treatment liquid, and the saliva sample treatment liquid comprises the following components: a zwitterionic anti-adsorption buffer system, an antibody conformation relaxation agent, a chelating agent type colloid redispersant, hydrophilic small molecular protein and a cyclodextrin type hydrophobic shielding agent. According to the method, systematic improvement is performed from the biochemical performance of the sample and the state of the antigen target, so that the defects that the target is covered by the colloidal environment of saliva and the target is lost due to aggregation of the target antigen are effectively overcome, and the detection sensitivity is greatly improved.

The application provides a primer, a probe and application of an RPA-gold colloidal lateral flow immunochromatography method for detecting tobacco bacterial wilt bacteria. The primer comprises a specific primer pair designed for a tobacco bacterial wilt bacteria genome ABNQ24_03690-lpxC gene interval sequence; the probe comprises a gold nanoparticle combined probe, a quality control line capture probe and a tobacco bacterial wilt bacteria capture probe. Meanwhile, application of the primer and the probe in preparation of a tobacco bacterial wilt bacteria detection product and a detection method are provided. The specific primer and the probe designed for the tobacco bacterial wilt bacteria genome ABNQ24_03690-lpxC gene interval sequence are fused with the rapid isothermal amplification technology of RPA and the visual detection advantage of the gold colloidal lateral flow immunochromatography, rapid, high-sensitivity and high-specificity detection of the tobacco bacterial wilt bacteria is realized, complex instruments and professional operations are not needed, and the method is suitable for early, convenient and rapid diagnosis in multiple scenes.

The present application relates to the fields of biotechnology and food detection technology, and discloses a rice flower honey colloidal gold immunochromatographic test strip as well as a preparation method and application thereof. Through proteomic analysis, the Ycf1 protein of chloroplast origin is identified as a specific marker of the plant source in rice flower honey, and a rice flower honey colloidal gold immunochromatographic test strip is developed. The test strip comprises a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad which are connected in sequence. The gold label pad is coated with gold-labeled antibodies formed by the combination of a monoclonal antibody against a specific segment of the Ycf1 protein and colloidal gold. The nitrocellulose membrane is provided with a detection line and a quality control line. The detection line is coated with a monoclonal antibody against the Ycf1 protein, and the quality control line is coated with a goat anti-mouse IgG. The test strip is simple to operate, and the detection can be completed within 15 minutes. The test strip has high sensitivity, strong specificity, and the results are intuitive and reliable, and is suitable for the on-site rapid screening and identification of the authenticity of rice flower honey.

The application belongs to the technical field of colloidal gold immunochromatography, and relates to cetirizine monoclonal antibody, nucleic acid molecules, vectors, detection kits and application thereof. The cetirizine monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises VH-CDR1, VH-CDR2 and VH-CDR3, the light chain variable region comprises VL-CDR1, VL-CDR2 and VL-CDR3, the sequence of VH-CDR1 is shown as SEQ ID NO. 1, the sequence of VH-CDR2 is shown as SEQ ID NO. 2, and the sequence of VH-CDR3 is shown as SEQ ID NO. 3. The monoclonal antibody of the application has high sensitivity to cetirizine, the minimum detection limit is 0.007 ng / mL, the quantitative range is 0.017-0.282 ng / mL, and the specificity is strong.

Systems and method for use in measuring lead levels in biological samples is provided. Using square wave coulometry and colloidal metallic particles impregnated on printed carbon electrodes on a sensor, the system provides a rapid, reliable, portable and inexpensive means of detecting low lead levels.

This invention provides a method and device for interpreting colloidal gold test strips. The detection method includes: after the test strip collects the sample to be tested, waiting for the test strip to complete the reaction; moving the test strip or the collection device so that the detection area or the sensor collection area moves along a first direction in a preset manner, wherein the collection device collects the reflected light signal of the test strip in a preset manner to obtain signal data covering the control part and the detection part; the collection device extracts the signal data and calculates the abrupt change characteristic value of the signal data to obtain the detection result.

The present application relates to the technical field of in vitro detection, and more particularly to a colloidal gold test strip for detecting pig whole blood, wherein the sample pad of the colloidal gold test strip is coated with a complex antibody of anti-pig red blood cell antibody and anti-pig hemoglobin antibody, which can directly bind to red blood cells and hemoglobin in pig whole blood, eliminating the interference of hemolysis in whole blood detection on the result. The colloidal gold test strip has the advantages of simple operation, good serum separation effect, small sample dosage, fast detection speed and the like, and has a wide application prospect in the field detection of pig epidemic.