10 results about "Paraquat" patented technology

The invention discloses a method for determining a quaternary ammonium salt pesticide in blood by using liquid chromatography-mass spectrometry, which comprises the following steps of: drawing a whole blood (plasma and serum) matrix labeling standard working curve through liquid chromatography-mass spectrometry analysis; obtaining peak areas of quantitative ion peaks of target objects containing paraquat, aquacide, diguat, chlormequat and mepiperidine with different concentrations and peak areas of quantitative ion peaks of internal standard substances, and further drawing a standard working curve; and performing liquid chromatography-mass spectrometry analysis on the whole blood sample under the same condition to obtain the peak area of a target substance quantitative ion peak and the peak area of an internal standard substance quantitative ion peak in the whole blood test solution, and calculating the content of each quaternary ammonium salt pesticide according to the standard working curve. The method can be used for simultaneously detecting the content of five quaternary ammonium salt pesticides in the whole blood of the poisoned patient simply, conveniently and quickly, and has the characteristics of simplicity, accuracy, good repeatability and high sensitivity.

The present disclosure provides a reagent and a method for detecting paraquat in herbicide preparation, the reagent comprising reagent A, reagent B and reagent C, the reagent A being a solution prepared from dipotassium hydrogen phosphate and an alkaline buffer, the reagent B being methanol, and the reagent C being a solution of acetylphenylhydrazine. The method comprises mixing the herbicide preparation to be tested with the reagent A to form a uniform solution A, then adding the reagent B to the uniform solution A, shaking and waiting for phase separation, taking the supernatant B and adding it to the reagent C, immediately visually comparing the color or using a spectrophotometer to compare the color, and determining whether the herbicide preparation to be tested contains paraquat according to the color change. The present disclosure uses acetylphenylhydrazine as a reducing agent combined with solvent to control the redox potential of the reducing agent, develops a detection system specific for reducing paraquat, and applies it to the detection of latent ingredients in herbicide preparations, which has the characteristics of high detection sensitivity, convenient operation and obvious phenomenon, and is suitable for on-site detection.

This invention relates to a paraquat granule and its preparation method, belonging to the field of pesticide technology. The granule consists of paraquat salt, a layered double hydroxide carrier, a disintegrant, microcrystalline cellulose, an outer coating of an emetic / odorant / tracking agent, and the remainder being water-soluble filler. The granule wear rate is ≤2%, it completely disintegrates within 60 seconds in standard hard water at 25℃, and the suspension rate is ≥90%. Preparation employs a one-step process of screw extrusion-centrifugal granulation-vacuum belt drying or fluidized bed granulation, resulting in high content retention. The product is dust-free and non-caking, suitable for drone-based aerial spraying and long-distance sea transport, facilitating large-scale application in overseas registered areas.

The invention discloses a ratio type fluorescent probe FITC-Lip-Au NCs and a preparation method and application thereof.The preparation method comprises the steps that lipase serves as a template, chloroauric acid serves as a metal precursor, a reaction is conducted under the alkaline condition to generate a red fluorescent gold nanocluster, the red fluorescent gold nanocluster is further coupled with fluorescein isothiocyanate, and the ratio type biological fluorescent probe with double emission peaks is obtained. The probe shows high selectivity and high sensitivity to paraquat and the like, the detection limit of paraquat is as low as 15 nM, good linear relations are shown in the ranges of 0.025-10 [mu] M and 10-45 [mu] M respectively, and the probe shows excellent quantitative detection capacity; based on the sensitivity response of the probe to paraquat, an application device of test paper and cotton swabs is constructed in combination with artificial intelligence, the fluorescence intensity ratio (R / G) of a red channel to a green channel of the test paper and the paraquat concentration are in a good linear relation in a specific range, and the paraquat is visually, sensitively and quantitatively detected. The defects of complex process, high cost, long time consumption and the like of the existing detection method are overcome.

The invention belongs to the field of plant anti-herbicides, and discloses a plant anti-paraquat herbicide PUT mutant protein and application thereof. According to the invention, transmembrane structural domain analysis is carried out on proteins PUT1, PUT2 and PUT3 of paddy rice, so that potential mutation sites PUT1G458, PUT2G359 and PUT3G444 of the paddy rice OsPUT1 / 2 / 3 gene are predicted. According to the present invention, the OsPUT1 / 2 / 3 gene is subjected to precise mutation by using the guide editing system method, the conserved site is mutated into the positively charged PUT1G458R, PUT2G359R or PUT3G444R so as to successfully obtain the target mutant plant, and the paraquat resistance identification is performed to finally create the paraquat-resistant rice germplasm, such that the important significance is provided for the diversity of the rice germplasm resource.

This invention discloses a bismuth ferrite / bismuth vanadate photo-Fenton catalyst, its preparation method, and a wastewater treatment device, belonging to the field of wastewater treatment technology. This invention prepares the bismuth ferrite / bismuth vanadate photo-Fenton catalyst via a sol-gel method, which can solve the problem of Fe in traditional Fenton technology. 3+ / Fe 2+ In addition to addressing the technical problem of cyclic obstruction, the photo-Fenton catalyst of this invention has the characteristic of wavelength-selective degradation, which can generate different active species under light conditions with wavelengths <450nm and wavelengths >600nm respectively, thereby achieving segmented and efficient removal of different types of pesticides such as imidacloprid, paraquat, carbofuran, and methomyl from wastewater.

The invention belongs to the technical field of wastewater treatment, and particularly discloses a paraquat wastewater resourceful treatment method which comprises the following steps: placing pyridine production wastewater under a subcritical condition, taking air as an oxidizing agent, enabling pyridine and derivative organic matters thereof to react on the surface of a noble metal catalyst, and degrading the organic matters into CO2, N2 and water; adsorbing residual pyridine at normal temperature by using special resin; and treating by adopting a biochemical process, and discharging after reaching the standard. Adjusting the pH value of the paraquat production wastewater to be acidic; the method comprises the following steps: placing wastewater under a subcritical condition, degrading nitrogen-containing organic matters into CO2, ammonia and water, and removing most cyanide and paraquat; the ammonium chloride and the sodium chloride are separated by hot circulating evaporation, and the recovered ammonium chloride and the recovered sodium chloride meet the recycling standard. By adopting the paraquat wastewater resourceful treatment method disclosed by the invention, the problems that pyridine compounds are difficult to degrade, the biochemical inhibition property is strong, and salt and pyridine resources cannot be effectively recycled in paraquat wastewater treatment in the prior art are solved.

The application belongs to the field of plant herbicide resistance, and discloses a plant paraquat herbicide-resistant PUT mutant protein and application thereof. Through transmembrane domain analysis on rice PUT1, PUT2 and PUT3 proteins, potential mutation sites PUT1G458, PUT2G359 and PUT3G444 of the rice OsPUT1 / 2 / 3 gene are predicted. The OsPUT1 / 2 / 3 gene is precisely mutated by using a guide editing system method, the conserved sites are mutated into positive charges PUT1G458R, PUT2G359R or PUT3G444R, the target mutant plants are successfully obtained, and the paraquat resistance is identified, and finally, the paraquat-resistant rice germplasm is created, which has important significance for rice germplasm resource diversity.

The invention discloses a method and a system for recovering paraquat cations from wastewater, and belongs to the technical field of wastewater treatment and resource recovery, and the method comprises the following steps: pretreatment, preparation of special exchange resin, resin adsorption, desorption and refining. The invention solves the problems of low recovery efficiency, high cost, easy secondary pollution, environmental unfriendliness and limited resource utilization value of concentrated ammonium salt when the paraquat cation-containing wastewater is treated in the prior art. By modifying the cation exchange resin and optimizing the resin adsorption and desorption process, the recovery rate of paraquat cations in the wastewater can reach 99% or above, efficient recycling of resources is effectively achieved, and compared with traditional methods such as concentration incineration, energy consumption is low, no harmful gas is discharged in the whole treatment process, and the method is suitable for industrial production. And the content of pollutants in the wastewater is reduced, so that the finally discharged wastewater reaches the environmental protection standard, and the method is simple and convenient to operate and has a good application prospect.

The invention discloses an SERS-ICA method for simultaneously detecting paraquat and carbaryl, and relates to the technical field of rapid detection of multiple pesticide residues. The SERS-ICA test strip is composed of a water absorption pad, a nitrocellulose membrane (NC membrane) marked with a detection line (T line) and a control line (C line), a sample pad and a support base plate (PVC). In order to perform multiple detection on a detection line (T line), two Raman reporter molecules 5, 5 '-dithiobis (2-nitrobenzoic acid) (DTNB) and 4-mercaptobenzoic acid (4-MBA) are respectively connected to the surface of the gold nanostar, and then are respectively connected with a paraquat monoclonal antibody (PQ mAb) and a carbaryl monoclonal antibody (CB mAb), so that two Raman active immune probes are prepared. Then, a test strip with two antigens sprayed and mixed on a T line is prepared and used for a chromatographic experiment, and target detection objects are distinguished by utilizing different Raman characteristic peaks of two Raman reporter molecules, so that high-sensitivity detection of two pesticides paraquat and carbaryl in one-time detection is realized.