The present invention provides a method for producing a
plant resistant to herbicides, the method expressing a base editing composition or base editor in a target
plant or part of the
plant, and growing or culturing the plant or part of the plant, the base editing composition has an activity of editing an adenine (A) base of
a DNA encoding a plant
chloroplast D1
protein into a
guanine (G) base and / or editing a
cytosine (C) base into a
thymine (T) base. The herbicide is preferably a
triazine herbicide, and more preferably
atrazine. A plant with resistance to the herbicide is a plant in which a
serine residue of the D1
protein is mutated into
glycine or an
alanine residue is mutated into
valine, the
serine residue is a
serine residue corresponding to a 264th-site serine residue of the D1
protein of
Arabidopsis thaliana, and the
alanine residue is a serine residue corresponding to a 264th-site serine residue of the D1 protein of the
Arabidopsis thaliana and is a serine residue corresponding to a 264th-site serine residue of the D1 protein of the
Arabidopsis thaliana and a
valine residue corresponding to the 264th-site serine residue of the D1 protein of the
Arabidopsis thaliana. The
alanine residue is an alanine residue corresponding to the 251 alanine residue of the D1 protein of the
arabidopsis thaliana. The base editing composition or base editor may comprise one or more fusion proteins, the one or more fusion proteins may each independently comprise
a DNA binding protein, and the
enzyme protein may comprise at least one of a
cytosine deaminase, an adenine deaminase, and a
nicking enzyme. The plant produced by the method is obtained by editing
a DNA sequence of 5 '-AGT-3' of a serine residue (a serine residue corresponding to a 264th-site serine residue of D1 protein of
arabidopsis thaliana) of encoding
chloroplast D1 protein into 5 '-GGT-3' of encoding
glycine, and editing a
DNA sequence of 5 '-AGT-3' of encoding
chloroplast D1 protein into 5 '-GGT-3' of encoding
glycine. Or the
DNA sequence encoding the 5 '-GCT-3'of the alanine residue is edited into an initial
gene edited plant encoding the 5'-GTT-3 'of
valine, or the
DNA sequence encoding the 5'-GCT-3 'of the alanine residue is edited into a 5'-GTT-3 'of valine. The invention provides a genetically edited plant or part of a plant, a progeny or seed thus obtained, and a method for controlling weeds by applying herbicides in the vicinity of these plants.