Fungicide used for degrading triazine herbicides and preparation method of fungicide

A technology of triazines and herbicides, which is applied in the field of triazine herbicides degrading bacterial agents and its preparation, can solve the problems of residual pollution of triazines herbicides, achieve great biological restoration significance and economic benefits, and reduce damage , the effect of low production cost

Active Publication Date: 2018-08-17
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve problems such as residual pollution of triazine herbicides, the object of the present invention is to provide a bacterial agent for degrading triazine herbicides and a preparation method

Method used

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  • Fungicide used for degrading triazine herbicides and preparation method of fungicide
  • Fungicide used for degrading triazine herbicides and preparation method of fungicide
  • Fungicide used for degrading triazine herbicides and preparation method of fungicide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The operation steps for preparing the bacterial agent for degrading triazine herbicides are as follows:

[0038] (1) Prepare Pseudomonas stutzeri (Pseudomonas stutzeri sp.) Y-2 wet cells

[0039]Under sterile conditions, a small amount of Pseudomonas stutzeri sp. Y-2 was inoculated in a solid medium with an inoculation loop, and placed in a constant temperature incubator for 3 days in the dark, at a temperature of 30 ℃±1℃, to get activated strains; inoculate two loops of activated strains into 100mg L of drug-containing -1 , In a 250mL Erlenmeyer flask with 100mL of liquid enrichment medium, culture in a constant temperature shaker at 30°C±1°C for 36h at 150r / min to obtain a bacterial suspension; inoculate 2mL of this bacterial suspension into a container containing 100mgL -1 , 300mL of liquid culture medium in a 1000mL Erlenmeyer flask, 30°C±1°C, 150r / min constant temperature shaker for 36h, the bacterial suspension was centrifuged at 6000r / min for 10min, the supernat...

Embodiment 2

[0060] Degradation experiment of simazine by bacterial agent degrading triazine herbicides

[0061] 1 Degradation experiment of bacterium agent on simazine in water body

[0062] (1) Preparation of Mineral Salt Medium (hereinafter referred to as MSM):

[0063] MgSO 4 ·7H 2 O, 0.4 g; FeSO 4 ·7H 2 O, 0.002 g; K 2 HPO 4 , 0.2 g; (NH 4 ) 2 SO 4 , 0.2 g; CaSO 4 , 0.08 g; 1 L of distilled water; adjusted to pH 7, autoclaved at 121°C and 101KPa for 20 minutes for later use.

[0064] (2) In 20 mL of MSM containing 100.0 mg / L simazine, add 0.5 g of the prepared triazine herbicide-degrading bacteria agent, and cultivate 0, 3, 6, After 9, 12, 16, and 24 hours, extract and detect the residual concentration of simazine. At the same time, the microbial agent of the present invention without humidifying the bacteria was set as a blank control, and three repetitions were set for both the control and the treatment.

[0065] Experimental results such as figure 2 It can be seen th...

Embodiment 3

[0078] (1) Degradation experiments of bacterial agents on other triazine herbicides

[0079] To the 20mL MSM containing 50.0mg / L atrazine, terbuthine, propazine, atrazine, proprazine, atrazine and terbutyne respectively, add 0.5g of bacterial agent for degrading triazine herbicides, After culturing on a shaker at 30°C and 150r / min for 2 and 4 days, extract and detect the residual concentration of triazine herbicides. At the same time, the microbial agent of the present invention without humidifying the bacteria was set as a blank control, and three repetitions were set for both the control and the treatment.

[0080] The degradation of other triazine herbicides by the bacterial agent of table 4

[0081]

[0082]

[0083] The results are shown in Table 4. The bacterial agent for degrading triazine herbicides has a good degradation effect on atrazine, terbuthyl, and permethazine. After 2 days, the degradation rate of atrazine reached 99%. The degradation rate was more th...

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Abstract

The invention relates to a fungicide used for degrading triazine herbicides. The fungicide is prepared by taking sodium alginate, polyvinyl alcohol and active carbon as carriers, and embedding with pseudomonas stutzeri sp. Y-2; in the fungicide, the content of the wet thallus of the pseudomonas stutzeri sp. Y-2 is 20g / L, the content of polyvinyl alcohol is 140g / L, and the content of sodium alginate is 10g / L; the fungicide is in the form of spherical particles, the surface is smooth, the elasticity is good, the mechanical strength is good, and the permeability is good. The pseudomonas stutzerisp. Y-2 has a good degrading effect for several kinds of triazine herbicides, and can rapidly degrade the triazine herbicides including atrazine, terbuthylazine and the like containing the chlorine group (-Cl). The free thallus is lost easily in the environment, and has the poor adaptive capacity to the environment, therefore, the thallus needs to be embedded and immobilized to form the fungicide,the fungicide is applied to the water body or soil polluted by the triazine herbicides in practical production, and the fungicide has great significance and economic benefit in improving the water body and soil.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of organic pollution, and includes a triazine herbicide-degrading bacterial agent and its preparation method and application. Background technique [0002] The triazine herbicide simazine (2-chloro-4,6-Bis(ethylamino)-1,3,5-triazine) is a systemic selective herbicide, which has teratogenic and growth-affecting toxic effects on animals and plants , but due to its excellent effect of controlling farmland broad-leaved grass and gramineous grasses, its usage is increasing day by day, and the environmental problems caused by simazine also follow. In this patent, a simazine efficient degrading bacterium screened from the sewage of a pesticide factory is used for immobilization research. Through the selection of fixation methods and materials, the optimal conditions for the preparation of bacterial agents were studied for repeated use experiments and the determination of degradation spectr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/08C12N11/14C02F3/34A62D3/02C02F101/30C12R1/38
CPCA62D3/02C02F3/34C02F2101/306C12N1/20C12N11/08C12N11/10C12N11/14C12N1/205C12R2001/38
Inventor 吴祥为潘丹丹闫涛杨雪颖花日茂
Owner ANHUI AGRICULTURAL UNIVERSITY
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