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Triazine herbicide residual degrading strain and strain therefrom

A technology for the degradation of pesticide residues and inoculants, which is applied in the field of high biology, can solve the problems of narrow degradation spectrum of strains and limited use, and achieve the effects of low production and use cost, increased added value, and good removal effect.

Inactive Publication Date: 2005-02-23
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, our research group already has an invention patent for degrading bacteria (ZL96106317.3, publication number: CN1145408A, publication date: March 19, 1997), which is a strain of Bacillus J5, which can effectively degrade methylamine Phosphorus, but the strain has a narrow degradation spectrum and can only degrade one pesticide, methamidophos, so its use is limited

Method used

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  • Triazine herbicide residual degrading strain and strain therefrom
  • Triazine herbicide residual degrading strain and strain therefrom
  • Triazine herbicide residual degrading strain and strain therefrom

Examples

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Embodiment Construction

[0018] Examples of the present invention are described below.

[0019] Activate the original seed of the microbial organophosphorus pesticide residue-degrading bacteria BTAH1 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seeds were inoculated into 1000ml shake flasks containing 200ml broth medium, cultured with constant temperature shaking until logarithmic phase, and prepared to inoculate seed tanks. 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, sterilize under high pressure at 121°C, cool to 33°C, inoculate the above cultured shake flask strains into a 500-liter seed tank at an inoculum size of 10%, and cultivate until the logarithmic growth phase, with a stirring ...

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Abstract

A method for preparing degrading bacteria for eliminating residual triazine herbicide is disclosed. The strain is Gram's chromatic reacting positive strain BTAH1, which belongs to exiguobacterium sp. Its characteristics are G+, irregular nemaline, 0.7-1.2X2.0-4.0mum, contact enzyme is positive, and it can hydrolyze starch. Genbank landing number of the strain 16S rDNA is AY205564. Its advantages include decrement of pesticide residual amount, atoxic and innoxious green agricultural product.

Description

1. Technical field [0001] The invention discloses a triazine herbicide residue degrading bacterium and the bacterium agent produced therefrom, which belong to the field of biological high technology, degrade chemical pesticide residues by means of microorganisms, and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Technical background [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, saved a lot of losses caused by diseases, insect pests and weeds for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production. In the process of modernization of agricultural production played an immeasurable role. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also ca...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 李顺鹏胡江崔中利代先祝何健
Owner NANJING AGRICULTURAL UNIVERSITY
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