Bactericide used for degrading triazine herbicides and preparation method thereof

A herbicide and triazine technology, which is applied in the field of triazine herbicide degrading bacteria and its preparation, can solve problems such as residual contamination of triazine herbicides, and achieve great bioremediation significance, economic benefits and production costs. Low, damage-reducing effect

Active Publication Date: 2016-09-07
安徽省公众检验研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve problems such as residual pollution of triazine herbicides, the object of the...

Method used

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  • Bactericide used for degrading triazine herbicides and preparation method thereof
  • Bactericide used for degrading triazine herbicides and preparation method thereof
  • Bactericide used for degrading triazine herbicides and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The operation steps for preparing the bacterial agent for degrading triazine herbicides are as follows:

[0031] (1) Preparation of wet bacterial cells of Leucobacter sp. JW-1

[0032] Under sterile conditions, a small amount of Leucobacter sp. JW-1 was inoculated in a solid medium with an inoculation loop, and placed in a constant temperature incubator for 4 days in the dark at a culture temperature of 30°C±1°C. Obtain activated strains; inoculate two loops of the activated strains into a 250mL Erlenmeyer flask filled with 100mL liquid enrichment medium, culture in a constant temperature shaker at 30°C±1°C, 150r / min for 40h, to obtain a bacterial suspension; The bacterial suspension was centrifuged at 6000r / min for 10min, the supernatant was discarded, and the precipitate was retained; the precipitate was washed 3 times with 0.9% sodium chloride solution to obtain wet bacterial cells of Leucobacter sp. JW-1.

[0033] The solid medium is beef extract peptone solid medi...

Embodiment 2

[0043] The Degradation Experiment of Promethazin by Bacteria Degrading Triazine Herbicides

[0044] 1 Degradation experiment of promethazin by bacterial agents degrading triazine herbicides in water

[0045] (1) Preparation of Mineral Salt Medium (hereinafter referred to as MSM):

[0046] MgSO 4 ·7H 2 O, 0.4 g; FeSO 4 ·7H 2 O, 0.002 g; K 2 HPO 4 , 0.2 g; (NH 4 ) 2 SO 4 , 0.2 g; CaSO 4 , 0.08 g; 1 L of distilled water; the pH was adjusted to 7.5, and the prepared MSM was autoclaved at 121°C for 20 minutes for later use.

[0047] (2) Add 0.3 g of the prepared triazine herbicide-degrading bacteria to 20 mL of MSM containing 100.0 mg / L proturon, and cultivate 0, 1, 2, After 3, 4, 6, 8, 10, and 12 hours, extract and detect the residual concentration of promethazine. At the same time, the microbial agent of the present invention without humidifying the bacteria was set as a blank control, and three repetitions were set for both the control and the treatment.

[0048] Ex...

Embodiment 3

[0056] The Degradation Experiment of Other Triazine Herbicides by Bacteria Degrading Triazine Herbicides

[0057] To 20mL of MSM containing 50.0mg / L of ametrazine, sigrazine, atrazine, permethazine, and simazine, respectively, add 0.5g of the bacterial agent for degrading triazine herbicides, and heat at 37°C, 150r / After culturing on a shaker for 2 and 4 days, extract and detect the residual concentration of triazine herbicides. At the same time, the microbial agent of the present invention without humidifying the bacteria was set as a blank control, and three repetitions were set for both the control and the treatment.

[0058] Table 1

[0059]

[0060] The results are shown in Table 1. The bacterial agents for degrading triazine herbicides have good degradation effects on atrazine, permethazine, terbuthylazine, simazine, atrazine and sizazone. After 2 days, atrazine , permethazine, terbuthine, arazine and sizazine were almost completely degraded, and the degradation ra...

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Abstract

The invention relates to a bactericide used for degrading triazine herbicides. The bactericide used for degrading the triazine herbicides is prepared by embedding Leucobacter sp. JW-1 in a crosslinked product, serving as a carrier, of sodium alga acid and polyvinyl alcohol; the content of wet vectors of the Leucobacter sp. JW-1 in the bactericide is 30 g/L, the content of polyvinyl alcohol in the bactericide is 120 g/L, and the content of sodium alga acid in the bactericide is 20 g/L; the bactericide used for degrading the triazine herbicides is in round particle shapes, smooth in surface, and good in elasticity, mechanical strength and permeability. The Leucobacter sp. JW-1 has an excellent degrading effect for the multiple triazine herbicides, and can be used for fast degrading the triazine herbicides containing SCH3 and Cl. Free Leucobacter sp. JW-1 is prone to running off and poor in environmental adaptability, and therefore the Leucobacter sp. JW-1 is embedded and fixed to be prepared to be the bactericide which is practically applied to the technical field of bioremediation of the triazine herbicides and has major bioremediation significance and economical benefits.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of organic pollution, and relates to a triazine herbicide-degrading bacterial agent and a preparation method and application thereof. Background technique [0002] Triazine herbicides are one of the most widely used herbicides in the world. In 2007, the global sales of triazine herbicides accounted for 5.3% of the herbicide market. There are currently 11 varieties on the market, such as atrazine, simazine, terbuthylazine, permethazine, promethazin, sigrazin, atrazine, metrizone, etc. Triazine herbicides are widely used in soybean, corn, sorghum and other crop fields to control gramineous and broad-leaved weeds. Triazine herbicides have stable chemical properties and strong adsorption in soil, so the half-life in the environment can reach several months or even more than a year. Triazine herbicides are easy to pollute soil, surface water and groundwater . Some studies have shown th...

Claims

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Application Information

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IPC IPC(8): A62D3/02A62D101/04A62D101/26
CPCA62D3/02A62D2101/04A62D2101/26
Inventor 吴祥为刘军委潘丹丹花日茂
Owner 安徽省公众检验研究院有限公司
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