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Self-assembled compounds of dyes and eight-membered cucurbit rings for use as fluorescent probes

An eight-membered cucurbit ring and fluorescent probe technology, applied in the application field as a fluorescent probe, can solve the problems of affecting the detection result, affecting the specific binding performance of the probe molecule and the target receptor, etc.

Inactive Publication Date: 2011-12-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the change of the structure of the compound generally requires complex organic synthesis steps, and the result may affect the specific binding performance of the probe molecule and the target receptor, and ultimately affect its detection results

Method used

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  • Self-assembled compounds of dyes and eight-membered cucurbit rings for use as fluorescent probes
  • Self-assembled compounds of dyes and eight-membered cucurbit rings for use as fluorescent probes
  • Self-assembled compounds of dyes and eight-membered cucurbit rings for use as fluorescent probes

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0021] A self-assembled compound composed of one of the fluorescent dyes acridine orange (AO), methylene blue (MB), pyronin Y (PYY), diaminoacridine (PF), Lowe's violet (TH) and eight-membered melon ring was used as The fluorescent probe is prepared into an aqueous solution from the following raw materials according to the following weight (w / w) percentages: fluorescent dye: 5%, eight-membered melon ring: 12%, and water to make up to 100%.

preparation Embodiment 2

[0023] A self-assembled compound composed of one of the fluorescent dyes acridine orange (AO), methylene blue (MB), pyronin Y (PYY), diaminoacridine (PF), Lowe's violet (TH) and eight-membered melon ring was used as The fluorescent probe is prepared into an aqueous solution from the following raw materials according to the following weight (w / w) percentages: fluorescent dye: 3%, eight-membered melon ring: 15%, and water to make up to 100%.

preparation Embodiment 3

[0025] A self-assembled compound composed of one of the fluorescent dyes acridine orange (AO), methylene blue (MB), pyronin Y (PYY), diaminoacridine (PF), Lowe's violet (TH) and eight-membered melon ring was used as The fluorescent probe is prepared into an aqueous solution from the following raw materials according to the following weight (w / w) percentages: fluorescent dye: 6%, eight-membered melon ring: 12%, and water to make up to 100%.

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Abstract

The invention discloses application of a dye and cucurbit [8] uril self-assembled compound as a fluorescent probe. After being prepared into an aqueous solution, the self-assembled fluorescent probe can be used for detecting target objects to be detected, such as pesticide paraquat, DNA (Deoxyribonucleic Acid), RNA (Ribonucleic Acid) and the like. The probe has the advantages that: the backgroundfluorescence of the probe is extremely weak, and the fluorescence can be obviously enhanced only after the fluorescent probe acts with a target object to be detected, so that excess probes are not required to be removed from a system.

Description

technical field [0001] The present invention relates to the application of a class of compounds, in particular to the application of a class of fluorescent dyes and eight-membered cucurbit [8]uril (CB[8]) self-assembled compounds as fluorescent probes. Background technique [0002] Fluorescent molecular probes are based on the organic combination of molecular recognition and fluorescence technology. Molecular recognition is achieved through the binding of probe molecules to specific target receptors, and then the information recognized by these probe molecules is converted into detectable fluorescence. signal, and then realize in situ and real-time detection at the single-molecule level. Unfortunately, the probe molecules themselves generally have strong fluorescence. In practical applications, the free probe molecules that are not bound to the target receptor must be removed from the system, otherwise the fluorescence of the free probe itself will produce strong fluorescenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/33G01N21/64
Inventor 孙世国刘凤玉李福胜
Owner NORTHWEST A & F UNIV
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