Paraquat-glyphosate dual detecting card and processing method of test sample thereof

A detection card and paraquat technology, which is applied in the field of equipment for detecting pesticide residues, can solve the problem of not having simultaneous detection and detection equipment, and achieve the effects of saving detection costs, fast detection, and convenient use

Inactive Publication Date: 2009-07-29
周坚
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Problems solved by technology

[0003] At present, the existing technology mainly relies on chromatographic analysis and enzyme immunoassay kits for the detect

Method used

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  • Paraquat-glyphosate dual detecting card and processing method of test sample thereof

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Embodiment Construction

[0013] The present invention is further explained as follows with reference to accompanying drawing in conjunction with specific embodiment:

[0014] A paraquat-glyphosate dual detection card, which is provided with a test strip 2 in the detection card casing 1, the structure of the detection card casing 1 and the setting of the test strip 2 are shown in the accompanying drawing 1, and the detection card casing 1 is long A flat thin shell-shaped shell with a length of 7cm, a width of 2cm, a thickness of 0.5cm, and a wall thickness of 0.1cm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0015] The test strip 2 is a thin strip with a multi-layer structure, the length of the strip is about 6.0cm, the width of the ...

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Abstract

The invention discloses a paraquat-glyphosate bigeminal detection card; the internal part of an outer shell has a test strip which is pasted with a nitrocellulose membrane in the middle of a supporting back plate; the two end parts are respectively pasted with an absorbent membrane and a sample pad; the inner ends are respectively overlapped with one end of the nitrocellulose membrane; a section of colloidal gold film which is a glass fiber membrane containing anti-paraquat and anti-glyphosate monoclonal antibody or polyclonal antibody colloidal gold marker is clamped between the overlapped part of the sample pad and the nitrocellulose membrane; the nitrocellulose membrane has 2 test strips which respectively contain a paraquat protein conjugate and a glyphosate protein conjugate; and a matrix pore area contains an anti-rabbit antibody or anti-mouse antibody. The sample pad and the nitrocellulose membrane respectively face a sampling hole and a detection window hole. The invention has the advantages that the paraquat and glyphosate contained in fruits, vegetables, and cereals can be simultaneously detected; the preparation is easy, the detection cost is saved, the use is convenient, the detection is rapid, the sensitivity is high and the result is accurate.

Description

Technical field: [0001] The invention relates to a device for detecting pesticide residues, in particular to a paraquat-glyphosate dual detection card and a processing method for detection samples thereof. Background technique: [0002] Pesticides are a special class of chemicals. According to literature reports, the utilization rate of pesticides is generally 10%, and about 90% remain in the environment. They are transferred to the human body through the enrichment of the food chain, causing harm to the human body. Paraquat and glyphosate are the earliest herbicides used in the world, and the fatality rate of long-term human intake is very high. In December 2008, a 16-year-old boy in Shanghai was severely poisoned by eating paraquat by mistake. According to the search, there have been hundreds of cases of death from poisoning by mistakenly eating "paraquat" in foreign countries, among which the conventional dosage form was 30ml. All the above 49 cases died. Glyphosate is ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N1/28
Inventor 周坚
Owner 周坚
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