Method using surface enhanced raman spectroscopy to detect paraquat

A surface-enhanced Raman and spectral detection technology, applied in Raman scattering, preparation of test samples, measurement devices, etc., can solve the problems of detection substrate interference, complex components, affecting the sensitivity of detection results, etc., and achieve low-cost detection, The effect of high sensitivity and low detection limit

Inactive Publication Date: 2017-05-31
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Beverages such as fruit juice and vegetable juice have complex components, which wil

Method used

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  • Method using surface enhanced raman spectroscopy to detect paraquat
  • Method using surface enhanced raman spectroscopy to detect paraquat
  • Method using surface enhanced raman spectroscopy to detect paraquat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation of gold nanoparticle sol

[0039]100mL concentration is that the chloroauric acid solution of 0.01wt% is poured in the flask, is placed on the constant temperature magnetic stirrer, 1100rpm vigorously stirs and is heated to solution boiling, then adds 0.7mL concentration and is the trisodium citrate solution of 1wt%, simultaneously Stir vigorously at 1100rpm and keep boiling for 15min. The reaction is quickly terminated in an ice bath. After the reaction solution is cooled, 15mL is taken for centrifugation and the supernatant (about 14mL) is discarded. The gold nanoparticle hydrosol in the lower layer is used for subsequent detection.

[0040] The prepared gold nanoparticles were detected, and the results were as follows: figure 2 , the average particle size of the prepared gold nanoparticles is 64±6nm, the size is uniform, and the dispersion is good.

Embodiment 2

[0041] Example 2 Detection of Paraquat Standard Solution

[0042] Firstly prepare paraquat standard aqueous solutions with gradient concentrations (10μg / L, 5μg / L, 2μg / L, 1μg / L, 0.5μg / L, 0.2μg / L, 0μg / L), and filter the standard aqueous solution with a 0.22μm filter membrane Finally, take 50 μL and place it in a 1.5 mL centrifuge tube, add an equal volume of gold nanoparticle sol (prepared in Example 1) to mix, vortex and shake for 10 seconds, take 5 μL of the mixed solution and add it dropwise to a clean silicon chip, and dry it. Surface-enhanced Raman spectroscopy was performed using a micro-Raman spectrometer. The power of the micro-Raman spectrometer is 80mW, the laser wavelength is 780nm, and the collected spectrum ranges from 550 to 2000cm -1 .

[0043] The test results of paraquat standard solution with gradient concentration are as follows: image 3 As shown, the Raman spectrum of paraquat standard is shown as figure 1 shown. according to image 3 It can be seen fr...

Embodiment 3

[0044] Example 3 Direct Determination of Paraquat in Apple Juice

[0045] Add paraquat mother liquor to apple juice, prepare apple juice samples of paraquat with a gradient concentration of 0-20 μg / mL and filter through a 0.22 μm filter membrane, take 50 μL and place it in a 1.5 mL centrifuge tube, add an equal volume of gold nanoparticle sol (implemented Example 1 (preparation and mixing), after shaking for 10s, take 5 μL and drop it on a clean silicon wafer, dry it, and use a micro-Raman spectrometer to measure it. The power of the micro-Raman spectrometer is 80mW, the laser wavelength is 780nm, and the scanning range is 550-2000cm -1 .

[0046] The obtained SERS spectrum is as follows Figure 6 As shown, it can be seen that the direct mixing method was used to determine the content of paraquat in apple juice, and the minimum detection concentration was 5 μg / mL.

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Abstract

The invention belongs to the field of pesticide detection, and provides a method using surface enhanced raman spectroscopy to detect paraquat. The method comprises the following steps of pretreating samples by weak anion ion exchange resin, such as fruit juice and vegetable juice, collecting an eluent, using gold nanometer particle sol as a substrate, and using the surface enhanced raman spectroscopy to detect the paraquat. The method has the advantages that the content of paraquat in the fruit juice and vegetable juice can be quickly and effectively detected, the sensitivity is high, the detection limit is low, and the paraquat with content of 0.02mug/mL can be effectively detected; the paraquat pesticide can be quickly and efficiently detected at low cost.

Description

technical field [0001] The invention relates to a method for detecting paraquat, in particular to a method for detecting paraquat by using surface-enhanced Raman spectroscopy. Background technique [0002] With the advancement of science and technology, the use of pesticides in agriculture has become increasingly widespread. However, pesticide residues in agricultural products are harmful to personal safety. Pesticide residues in agricultural products gradually accumulate in the human body after being eaten, causing chronic poisoning and endangering personal safety. [0003] As a quick-acting contact-killing quaternary ammonium salt herbicide, paraquat has high-efficiency herbicide effect and is widely used in the planting process of fruit and vegetable crops. At present, more than 130 countries and regions in the world are using pesticides containing paraquat in large quantities. Because paraquat has a stable chemical molecular structure and is not easy to degrade, it re...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/34
CPCG01N21/658G01N1/34
Inventor 樊玉霞黄轶群赖克强罗海瑞
Owner SHANGHAI OCEAN UNIV
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