155results about How to "Rapid quantitation" patented technology

The invention provides a human glutathione peroxidase (GPx6) and polynucleotides which identify and encode GPx6. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of GPx6.

The invention discloses a quantum dot multicolor marking method for quantitative detection of various cardiovascular disease markers and a kit of troponin I/creatine kinase isoenzyme/myohemoglobin. The method realizes fluorescent quantitative detection by utilizing excellent fluorescent properties of quantum dots and combining a multicolour marking technology and an immunochromatographic assay on the basis of optimizing each component of a test strip. Compared with the common collaurum immunochromatographic assay, the method has the advantages of good mark stability, low nonspecificity, high sensitivity, wide linear range, small cross interference, and accuracy in quantification. The kit disclosed by the invention is used for carrying out quantification detection on the troponin I, the creatine kinase isoenzyme and the myohemoglobin simultaneously, is suitable for detection of whole blood, blood serum and plasma samples, can provide a reference for cardiovascular and cerebrovascular disease diagnosis, and is widely applied to primary hospitals and clinics.

The invention provides human immune system associated proteins (HISAP) and polynucleotides which identify and encode HISAP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for treating or preventing disorders associated with expression of HISAP.

The invention discloses a fluorescence immunochromatographic assay and kit for quantitatively detecting acute myocardial infarction marker, namely, N-terminal pro brain natriuretic peptide (NT-proBNP). The fluorescence immunochromatographic assay for quantitatively detecting the NT-proBNP realizes fluorescence quantitative detection on the basis of optimizing various constituent parts of test paper by using excellent fluorescence characteristic of quantum dots and combining a bicolor marking technology and an immunochromatographic technology. Compared with the conventional colloidal gold immunochromatographic assay, the fluorescence immunochromatographic assay disclosed by the invention has the advantages of good marking stability, low non-specificity, high sensitivity, wide linear range and quantifying accuracy. The fluorescence immunochromatographic kit disclosed by the invention is used for carrying out quantitative detection on the NT-proBNP and detecting whole blood, blood serum and blood plasma samples, can provide reference for diagnosis of cardiovascular and cerebrovascular diseases and can be widely applied to primary hospitals and clinics.

The invention relates to the technical field of earthquake damage evaluation, in particular to a method for evaluating the overall earthquake damage level of a reinforced concrete frame structure based on an equivalent single-degree-of-freedom system. The method is used for quickly and accurately evaluating the overall earthquake damage level of the structure after an earthquake by using strong ground motion data acquired by the structure. The method includes the steps that a multi-degree-of-freedom system structure is equivalent to the single-degree-of-freedom system structure, and namely the multi-degree-of-freedom system with N layers is equivalent to the single-degree-of-freedom system, wherein the mass M[e] of the single-degree-of-freedom system is equal to the total mass of the multi-degree-of-freedom system; the maximum acceleration response of the equivalent single-degree-of-freedom system under the same earthquake ground motion effect is worked out; the ductility coefficient of the equivalent single-degree-of-freedom system under the same earthquake ground motion effect is worked out; damage index calculation is carried out; structural damage evaluation is carried out. By means of the method, the defects that large errors can be caused in a damage evaluation method based on modal parameters and time and labor are consumed and results are prone to divergence in a damage evaluation method based on physical parameters are overcome, and the overall earthquake damage level of the structure can be easily, quickly and effectively evaluated in a quantitative mode.

The invention discloses a method for rapid detection and authenticity identification of fatty acid of racy camellia oil by near infrared transmission spectroscopy (NITS). The method comprises the steps of: screening high quality varieties with oil content of 30%-60% from more than 130 camellia seed varieties, wherein in the camellia oil, the content of oleinic acid is 65%-85%, the content of linoleic acid is 5%-20%, the content of palmitic acid is 3%-15%, and the content of stearic acid is 1%-5%; establishing a near infrared transmission spectroscopy (NITS) model of the main fatty acids (oleinic acid, linoleic acid, palmitic acid, stearic acid and total unsaturated fatty acid) with a partial least squares (PLS) method by adopting the Fourier near infrared transmission spectroscopy (NITS), wherein the determination coefficients of the correction models of the oleinic acid, the linoleic acid and the palmitic acid respectively are 0.93446, 0.96538 and 0.88789, and the determination coefficients of cross validation respectively are 0.91987, 0.95755 and 0.84447; further verifying accuracy and reliability of five NIRS (Near Infrared Reflectance Spectroscopy) models by external verification so as to obtain related coefficients of the external verification, which respectively are 0.9424, 0.9682, 0.8862, 0.6834 and 0.7587; and evaluating doped camellia oil by establishing the NIRS model of binary system miscella of the camellia oil. The method is applicable to rapid measurement of the content of main fatty acids of the camellia oil and the doped camellia oil, has the characteristics of simplicity, convenience, rapidness and economy, and is suitable for industrial application.

A method for evaluating the integrity of microfiltration, ultrafiltration and nanofiltration membranes, which method comprises passing a liquid that contains a substantially mono-dispersed population of nano-probes through said membrane to form a permeate and testing said permeate for the presence of said nano-probes, wherein the non-detection of said nano-probes in said permeate indicates that said membrane is substantially intact.

The invention provides a human signal peptide-containing proteins, the polynucleotides which encode them and methods for their use. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention further provides methods for diagnosing or treating disorders associated with expression of the proteins

The invention provides a method for quantitatively discriminating nephrite production areas. The method includes the steps that firstly, nephrite samples of known production areas are prepared; secondly, the content of microelements of the samples is quantitatively tested, and test points are divided into a training group and a testing group; thirdly, the content of the microelements is subjected to significance level analysis to detect whether statistic differences exist between different production areas; fourthly, by means of the binary iteration linear discriminant analysis (IB-LDA) method, data of the training group are discriminated two by two, and feasibility of the method is tested through the testing group; fifthly, the production areas of the samples to be tested are predicted through the IB-LDA method. The method quantitatively discriminates the nephrite production areas on the basis of binary iteration linear discriminant analysis of the microelements, the mode that multiple traditional groups are compared at the same time is simplified into the mode that the groups are compared two by two, and the discrimination probability of each production area relative to other production area is obtained. Besides, the discrimination ability of a discrimination function is remarkably improved. Compared with the naked eye qualitative discrimination method and a traditional linear discriminant analysis method, the method has obvious advantages.

The invention discloses a kit and method for quantitatively detecting five fat-soluble vitamins in blood plasma. The kit comprises a fat-soluble vitamin calibrator, an isotope mixed standard substance, a fat-soluble vitamin quality control substance, an instrument quality control substance and a mobile phase additive. According to the invention, a solid-phase support liquid-liquid extraction method is adopted and is matched with an automatic pipetting station, so a sample pretreatment method is greatly simplified, and the aim of simultaneously and accurately detecting the five fat-soluble vitamins at high flux and high speed is fulfilled by combining a liquid chromatography-tandem mass spectrometry method. According to the invention, during the detection of a peripheral blood plasma sample, five fat-soluble vitamins can be quantified at a time only by about 250 microliters of plasma sample; moreover, due to a fact that the pretreatment method is simple and quick, the operation time issaved, the content of the fat-soluble vitamins in a human body can be effectively diagnosed and detected according to the indexes, and diseases caused by lack or excess of the fat-soluble vitamins canbe prevented and diagnosed in time. Therefore, the kit and method have extremely high application values in clinical, health examination and scientific research.

The invention discloses a direct fungus detection tachypleus amebocyte lysate box and a direct fungus detection method. The kit comprises: (1) a G reagent which is a mixture of lichaampje cytolyzate and color development material of tachypleus; (2) buffer solution which is 0.02 to 0.1mol/L Tris-HCL; (3) a standard substance which is (1-3)-beta-D-glucan; (4) diazotizing agent; and (5) blood treating liquid. The (1-3)-beta-D-glucan content of a sample is calculating by processing the sample and the standard substance, preparing solution of the G reagent, filling, raising temperature, stopping, measuring a light-absorbing value and making a standard curve. The kit can quickly and accurately measure the micro (1-3)-beta-D-glucan content of human body blood and body blood samples.

The invention discloses a screening method of nucleic acid aptamers for specifically recognizing streptomycin. The screening method comprises the following steps: (A) coupling epoxy group gel with streptomycin; (B) preparing a negative electrode pole and a positive electrode pole; (C) constructing an ssDNA random library, and screening and determining aptamers with relatively high affinity to streptomycin; and (D) authenticating the aptamer with the highest affinity for specifically recognizing streptomycin by virtue of a fluorescence method. According to the screening method, the screened nucleic acid aptamer is utilized for qualitatively and quantitatively determining streptomycin in food. The screening method is high in condition preciseness, other interference factors are easily eliminated, the screened nucleic acid aptamer has very high affinity and high specific recognizability, and furthermore, a false positive phenomenon can be reduced; the screening method is a novel method for simply, rapidly, scientifically and accurately screening the nucleic acid aptamers; by combining the screened nucleic acid aptamer with a colloidal gold colorimetric method, streptomycin can be simply, rapidly and accurately detected; and the screening method can be widely applied to various fields of foods, sanitation, import and export inspection and the like.

This invention is related to a method and assay kit for rapidly quantifying global DNA methylation through immobilizing DNA by simple dry-capture on the plastic carrier followed by immunodetection of 5-methylcytosine structure that is the marker of DNA methylation.

The invention discloses a kit for detection of acute T-lymphocytic leukemia naive T cells, application and a method thereof, and belongs to the field of biological medicine. The adopted technical scheme is as below: the kit for the detection of acute T-lymphocytic leukemia naive T cells can be applied to a flow cytometry, and includes monoclonal antibodies aiming at the following materials: TDT, ccd3, CD34, CD5, CD7, mCD3 and CD45. The antibodies respectively have fluorescent labels. The invention also provides the application of the above kit to the preparation of products for diagnosis of acute T-lymphocytic leukemia. The invention has the beneficial effects that: (1) the kit provided by the invention can quickly and accurately conduct analysis and quantification on trace LBTC antigen of all T-ALL patients; and (2) the gating method and multiple antigen markers integral evaluation provided by the kit has high specific degree and high sensitivity.

The invention discloses a method of determining nitrogen fertilizer application in crops, which can determine the nitrogen fertilizer application Nfert of the crops according to the quantitative relation of nitrogen fertilizer, soil nitrogen and nitrogen uptake by the crop at the crop root zone. The method can realize quick, accurate and quantitative nitrogen fertilizer application without testing the soil or plants, so that the cost for determining the nitrogen fertilizer application in the production is greatly saved.

The invention discloses a method for detecting blue algae capable of producing 2-MIB by PCR qualification and TaqMan fluorescent quantitative PCR quantity. The method comprises the following steps: water sample to be measured is collected, filtered through a cellulose acetate film, extracted and purified total genome DNA on the filtered algae; B, a qualitative PCR reaction system comprises: corresponded monoterpene cyclase gene is synthesized, 2-MIB blue algae is taken as positive control and double distilled water is taken as negative control; C, a qualitative PCR reaction progress comprises: denaturation is carried out at the temperature of 94 DEG C for 3 minutes, circulated and finally extended; D, qualitative PCR products and positive control, negative control; E, the fluorescent quantitative PCR reaction system, aseptic double distilled water is added in negative template control; F, the fluorescent quantitative PCR reaction progress; G, stand curve making; H, the concentration of blue algae capable of producing 2-MIB in the water sample is calculated according to the Ct value and the standard curve of the sample to be measured. The method of the invention has the advantages of easy process, simple operation, high sensitivity and accuracy, so that the concentration of blue algae capable of producing 2-MIB in the water sample can be rapidly and accurately quantified.

The invention provides a human protein kinase C inhibitor (HPKCI) and polynucleotides which identify and encode HPKCI. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for treating or preventing disorders associated with expression of HPKCI.

The invention belongs to the field of in-vitro diagnosis, and particularly relates to a test strip card which can filter samples and is provided with a storage medium (14) for storing a tested object standard curve, coefficient parameters or other detection information. The test strip card comprises a card box (3) and a test strip (2) in the card box (3), wherein a storage medium (14) is installed on the card box (3). When detecting a sample, an instrument with signal detection function acquires characteristic signals of a test strip (2) detection belt (7) and calculates the sample single component or multicomponent concentration in combination with the tested object standard curve or coefficient parameters simultaneously read from the storage medium (14) by the instrument. The test strip card for detecting samples has is simple and quick, and has the characteristics of high sensitivity, objective result, high use flexibility and the like.

The invention discloses a fluorescence immunochromatographic assay and kit for quantitative detection of acute myocardial infarction marker-creatine kinase isoenzyme (CK-MB). The fluorescence immunochromatographic assay for quantitative detection of the CK-MB realizes fluorescent quantitative detection by utilizing excellent fluorescent properties of quantum dots and combining a bicolour marking technology and an immunochromatographic assay on the basis of optimizing each component of a test strip. Compared with the common collaurum immunochromatographic assay, the fluorescence immunochromatographic assay has the advantages of good mark stability, low nonspecificity, high sensitivity, wide linear range and accuracy in quantification. The kit disclosed by the invention is used for carrying out quantification detection on the CK-MB, is suitable for detection of whole blood, blood serum and plasma samples, can provide a reference for cardiovascular and cerebrovascular disease diagnosis, and is widely applied to primary hospitals and clinics.