75 results about "Monoterpene" patented technology

The invention discloses application of protein and related biological materials thereof in promoting accumulation of grape fruit monoterpene in the field of variation or genetic engineering. The technical problem to be solved by the invention is how to improve the monoterpene content of grape fruits. A technical scheme to be protected by the invention is application of a protein with an amino acid sequence as a sequence 2 in a sequence table in increasing the content of grape fruit monoterpenes. Experiments prove that VvHYH from grapes can promote accumulation of monoterpene substances of grape fruits and leaves, and the VvHYH can be applied to grape flavor improvement and breeding.

The present invention relates to an alcohol acyltransferase capable of esterifying a tertiary monoterpene alcohol, preferably within 36 hours, 24 hours, 18 hours, 12 hours, 6 hours, 3 hours, 2 hours, 1 hour, 45 minutes, or 30 minutes, more preferably in a microbial cell, so that at least 30% by weight of the tertiary monoterpene alcohol is esterified. The present invention further relates to a nucleic acid comprising a nucleic acid sequence encoding the alcohol acyltransferase of the present invention or a complementary sequence thereof, and a vector or genetic construct comprising the nucleic acid of the present invention. The present invention also provides a host cell comprising the vector or genetic construct of the present invention, and a transgenic non-human organism comprising the nucleic acid of the present invention, the vector or genetic construct of the present invention, or the host cell of the present invention. The present invention also relates to a method for preparing a monoterpene ester, which method comprises esterifying a monoterpene alcohol to a monoterpene ester in the presence of the alcohol acyltransferase of the present invention. Specifically, it provides a method for preparing linalyl acetate, which comprises esterifying linalool to linalyl acetate in the presence of the alcohol acyltransferase of the present invention.The present invention further provides (i) heterologous reconstitution of terpene biosynthetic pathways; (ii) for producing industrial products, preferably flavors or fragrances, biofuels, fuel compositions, fuel compounds, e.g., foaming agents for diesel fuel compositions, pesticides, insect repellents or antimicrobial agents; (iii) for producing aliphatic and / or aromatic monoterpene esters from monoterpene alcohols, preferably from tertiary monoterpene alcohols; (iv) for detoxifying monoterpene alcohols in microorganisms, thereby increasing monoterpene production in said microorganisms; (v) for combining monoterpene alcohols with GPP synthase and / or S- or R-linalool synthase; (vi) to enhance the beneficial effect of acetylation in that the hydrophobic acetate partitions more readily into the organic phase compared to the monoterpene alcohol; (vii) to express the alcohol acyltransferase of the invention so that the ratio of monoterpene acetate to monoterpene alcohol is greater than 5:1 or 10:1; or (viii) in a microbial production system for monoterpene esters. The invention also provides kits comprising the alcohol acyltransferase of the invention, the nucleic acid of the invention, the vector or gene construct of the invention, the host cell of the invention, or the transgenic non-human organism of the invention, and optionally at least one monoterpene alcohol, preferably a tertiary monoterpene alcohol.Finally, the present invention relates to a method for the production of fuel and / or biolubricant compounds, the method comprising: a) producing one or more monoterpene esters by any one of the methods of the present invention; b) optionally purifying the one or more monoterpene esters produced in step a); and c) converting some or all of the one or more monoterpene esters of step a) or the purified one or more monoterpene esters of step b) into a suitable product, preferably: tetrahydrolinalool; 2,6-dimethyloctane (DMO); saturated C20 hydrocarbon dimers; saturated C30 hydrocarbon trimers; hydrogenated methylcyclopentadiene dimers; and hydrogenated C40+ oligomers suitable for producing a biolubricant additive; and d) optionally combining the one or more fuel or biolubricant compounds with additional compounds suitable for fuels and / or biolubricants; wherein the fuel and / or biolubricant composition has, in total, from 0.01% (w / w) to 99.99% (w / w) of the fuel or biolubricant compound produced from one or more monoterpene esters obtainable by one of the methods of the present invention.

The invention discloses a method for increasing the yield of monoterpene glycoside in an endophytic fungus fermentation product separated from paeonia lactiflora, and relates to the technical field of plant fermentation. The method comprises the following steps: material treatment; separating strains; identifying strains; preparing a strain and an elicitor: drying and crushing the overground part of the Chinese herbaceous peony by using the endophytic fungus ER6 separated and identified in the step S3, and sieving with a 80-mesh sieve to prepare powder as a key elicitor; a potato glucose liquid culture medium is used as a basic fermentation culture medium, an activated ER6 strain is inoculated into a fermentation container filled with a sterilized PDB culture medium, and dry powder of the overground part of paeonia lactiflora is added into the culture medium to serve as a biological elicitor to be cultured in a shaking table; and harvesting fermentation liquor. According to the method, a specific endophytic fungus ER6 is separated from paeonia lactiflora roots, and it is found that the yield of monoterpene glycoside in a fermentation product can be remarkably increased by using the overground part of paeonia lactiflora as a biological elicitor to ferment the endophytic fungus ER6.

The present invention relates to a water-absorbing resin and a method for manufacturing the same. The method for manufacturing the same is to perform a polymerization reaction on an acid group-containing monomer using a specific amount of a foaming agent under pressure sealing to obtain a core layer having a specific surface porosity, and then perform a surface cross-linking reaction on the core layer using an acyclic monoterpene alcohol having a carbon number of 10 to 12 and a surface cross-linking agent, so that the water-absorbing resin obtained has both good water-absorbing properties and superior deodorizing efficacy.

The application belongs to the technical field of biology, and discloses application of CmPHL1 transcription factor in inhibition of citrus leaf oil cell formation and regulation of volatile substances. In view of the problem of insufficient research on existing citrus oil cell formation and essential oil synthesis, a pGBI-CmPHL1 overexpression vector is constructed, and positive strains are obtained by means of Agrobacterium-mediated transformation of citrus. Experiments prove that overexpression of CmPHL1 can significantly reduce the number of oil cells and the content of monoterpenes, sesquiterpenes and other volatile substances. CmPHL1 targets the molecular regulation network of oil cell development, accurately regulates and is heritable, provides a new gene target for citrus quality improvement and breeding, is in line with the direction of green agriculture, and has important economic and ecological benefits.

Provided are a Cinnamomum burmannii monoterpene synthase CbTPS1, an amino acid sequence thereof, a nucleic acid molecule encoding the protein, an use thereof in preparing the monoterpene synthase, and a method for preparing dextrorotatory borneol by using the Cinnamomum burmannii monoterpene synthase CbTPS1.

Described herein are modified monoterpene TRPM8 activating compounds. In particular, provided herein are compounds that affect intracellular ion channel function, and are useful as or in conjunction with therapeutic agents. The compounds provided herein are useful in the treatment of a variety of diseases and disorders, including inflammatory eye diseases (e.g., uveitis), cardiovascular diseases, inflammatory diseases, and diseases characterized by aberrant hyperplasia (e.g., cancer).

This invention discloses a mango terpene synthase gene TPS4 and its applications, the nucleotide sequence of which is shown in SEQ ID NO:1. The amino acid sequence of the protein it encodes is shown in SEQ ID NO:2. The invention also discloses a recombinant expression vector containing the mango terpene synthase gene TPS4, a recombinant engineered strain, and the recombinant mango terpene synthase TPS4; and the application of the mango terpene synthase gene TPS4, its encoded protein, the recombinant expression vector, the recombinant engineered strain, or the recombinant TPS4 in regulating the synthesis of terpenoid compounds from geraniol diphosphate (GPP), neroli diphosphate (NPP), and farnesyl diphosphate (FPP). This invention is the first to clone and verify a terpene synthase gene TPS4 from the Sacred Heart mango genome, capable of simultaneously catalyzing the synthesis of monoterpenes and sesquiterpenes from three substrates: GPP, NPP, and FPP. It has broad application prospects and significant economic value.

Methods and systems for extracting additional beneficial lipid-soluble heavy compounds from plant material in an environmentally sustainable manner. A method includes preparing a solution that includes a solvent comprising one or more of a monoterpene, a monoterpenoid, a sesquiterpene, or a sesquiterpenoid. The solution further includes a solute comprising a plant material. The method includes extracting one or more compounds from the plant material using the solvent comprising the one or more of the monoterpene, the monoterpenoid, the sesquiterpene, or the sesquiterpenoid.

The application discloses a recombinant bacterium and a use thereof, the recombinant bacterium is a yeast containing or expressing aai-na-xiang monoterpene synthase BbTPS3 or an ai-na-xiang monoterpene synthase BbTPS3 fusion protein in vivo, and is used for producing levorotatory borneol; experiments prove that the recombinant bacterium after modification (such as codon optimization, protein truncation, Kozak increase or protein fusion) can improve the yield of levorotatory borneol, and is suitable for industrialized production of levorotatory borneol.

This invention belongs to the field of microbial metabolic engineering and biotechnology applications, specifically relating to an engineered bacterium for synthesizing monoterpenoid compounds, its construction method, and its applications. The construction method involves using *Hansenula polymorpha* as a host, regulating the mevalonate pathway in its cytoplasm, and overexpressing monoterpene synthase to obtain the engineered bacterium; or, overexpressing monoterpene synthase and the mevalonate pathway in peroxisomes to obtain the engineered bacterium. The engineered bacterium constructed in this application can efficiently synthesize monoterpenoid compounds such as geraniol, β-myrcene, and dextrorotatory borneol.

The invention relates to the technical field of traditional Chinese medicines, and provides a traditional Chinese medicine effective part composition for treating atopic dermatitis, a preparation as well as a preparation method and application thereof. The composition comprises a flavonoid extract of pimpinella thellungiana, an alkaloid extract of cortex dictamni, a saponin extract of fructus kochiae, a triterpenic acid extract of selfheal, a flavonoid extract of scutellaria baicalensis, a diterpenic acid extract of golden larch bark, a polysaccharide extract of angelica sinensis, a monoterpenoid glycoside extract of radix paeoniae alba, an alkaloid extract of cortex phellodendri and a coumarin extract of fructus cnidii. A sophora flavescens alkaloid extract and a honeysuckle phenylpropanoid extract; the traditional Chinese medicine preparation comprises the composition and auxiliary materials. The invention also discloses application of the composition and the preparation in preparation of medicines for treating atopic dermatitis. The medicinal preparation obtained by the invention can relieve pruritus, relieve inflammatory response, improve dry skin and relieve skin injury, thereby treating atopic dermatitis.

The present invention relates to a mixture, blend or combination, comprising at two, preferably at least three, chosen from the following compounds: (A) a pyrone derivative, (B) a tropolone derivative, (C) a phenylpropanoid, (D) a monoterpene or monoterpenoid, (E) an aromatic acid or salt, (F) a dipeptide, and (G) an alkanediol, and its use as chelating mixture.

The present invention relates to a process for producing biofertilizers / biostimulants from microalgae with intensive CO2 capture, which comprises the following steps:a) preparing an inoculum;b) cultivating for the production of microalgae biomass in a window photobioreactor;c) collecting the biomass by flocculation;d) drying the biomass, and grinding and extracting a polar fraction by solvent in a two-phase system; ande) analyzing the polar fraction by liquid chromatography.The invention also relates to the extracts of wet biomass of the microalgae Scenedesmus sp., obtaining the polar fraction. This polar fraction contains amino acids, sugars, betaine derivatives, vitamin B3 (nicotinamide or nicotinic acid) and pantothenic acid (vitamin B5), the monoterpene loliolide and the tryptophan derivative indole-lactic acid (analogous to indole-acetic acid, a phytohormone of the auxin class).The polar fraction can also be used as an input in the cultivation of soybeans, corn, sugar cane, cotton and coffee, among other cultivated species.

To provide an efficient method for producing a monocyclic monoterpene diol.SOLUTION: A method for producing a monocyclic monoterpene diol according to the present invention, comprises a step of hydrolyzing a monocyclic monoterpene oxide represented by the following formula (II) under acidic conditions. [In the formula, a double line composed of a dotted line and a solid line indicates a carbon-carbon single bond or a carbon-carbon double bond, and R1 indicates a 2-propenyl group, etc.]SELECTED DRAWING: None

A kind of hydrogenation bio-based terpene two-unit random copolymer elastomer and its preparation method and application belong to the field of olefin catalytic polymerization and hydrogenation.The purpose of the present application is to solve the bottleneck of POE industry development and the technical problem that the comprehensive performance of bio-based material is not high.The elastomer of the present application is copolymerized and hydrogenated by bio-based monoterpene and bio-based sesquiterpene, the molar content of monoterpene polymerization unit is 5-95%, the molar content of sesquiterpene polymerization unit is 5-95%, the hydrogenation degree is 90-99.9%, the number average molecular weight is 1.0-500,000 g / mol, and the molecular weight distribution is 1.0-5.0.The elastomer of the present application is used to prepare hot melt adhesive and its film products applied in photovoltaic solar and battery, automotive interior, clothing, shoe material, electronics, wall cloth, building, filtration or pharmaceutical field.

The present invention relates to the enhancement of flumetyl sulforim when it is utilized concomitantly, contemporaneously or in conjunction with Flumetylsulforim-Enhancing compounds, collectively, or typically, Isoprene unit-based compounds or compounds structurally based on 2-methyl-l,3-butadiene units or derived from them, such as, monoterpenoid and derivatives thereof, secondary metabolites originating from isoprene selected from Phenylpropanoids; Monoterpenoids, monoterpenes, flavones, Flavonoids etc., as part of a program to address agricultural and particularly crop plant pathogens, whether in curative, protective, and / or preventative programs. The present invention also relates to methods of increasing the enhancement of flumetylsulforim when it is utilized concomitantly, contemporaneously or in conjunction with Flumetylsulforim- Enhancing compounds collectively, or typically, Isoprene unit-based compounds or compounds structurally based on 2-methyl- 1,3 -butadiene units or derived from them, by adjusting the ratios between the flumetylsulforim and Flumetylsulforim-Enhancing compounds and / or by adjusting the concentration of the compounds in the spray mix from which they are applied.

The present application relates to the technical field of chemical analysis, in particular to a kind of intelligent evaluation method of organic matter emission flux in air, comprising: according to the difference between data in each cluster and each time period, obtain the isolated coefficient of each time period in relative abundance sequence;According to the difference between the isolated coefficient of all time periods of relative abundance sequence, the initial screening parameter of each time period is corrected, and the screening parameter after correction of each time period is obtained;According to the screening parameter after correction of each time period, obtain the artificial monoterpene concentration of each collection device at each time;According to the artificial monoterpene concentration of each collection device at each time, the flux evaluation of artificial monoterpene emission is carried out.The present application improves the accuracy of the prediction of the emission amount of artificial monoterpene, and improves the accuracy of the flux evaluation of artificial source monoterpene.

The invention discloses a monoterpene indole alkaloid compound as well as a preparation method and application thereof, and belongs to the technical field of medicines. The monoterpene indole alkaloid compound disclosed by the invention is separated from ervatamia palmata, has remarkable activity for repairing podocyte injury under the hypoxia condition, and can be used for preparing a medicine for treating chronic renal injury under the hypoxia condition. The traditional Chinese medicine composition has relatively high economic benefits aiming at the current situation of non-hypoxia chronic kidney injury specific medicines.

The invention discloses a preparation process of fatty alcohol polyoxyethylene ether sulfate with low residual organic solvent. The process comprises the following steps: cooling C12-C14 fatty alcohol-polyoxyethylene ether to 25 DEG C, introducing a polyol-monoterpene synergistic solvent, carrying out 7 kPa vacuum film evaporation at about 60 DEG C, blowing nitrogen microbubbles with the pressure of 0.2-0.4 MPa into a microwell plate with the pore diameter of 5-10 m, and carrying out enhanced desolvation according to a gas-liquid volume ratio of 0.6-1.0; sulfonating the obtained material with SO3 / air mixed gas subjected to graded cooling, neutralizing, continuously desolventizing through a thin-layer falling film (80-120 m) at the temperature of 45-50 DEG C, and scavenging with nitrogen; the whole process cooperates with mass transfer to drive 1, 4-dioxane and isopropanol to rapidly migrate, and the total residual solvent of the finished product is greatly reduced.

The invention belongs to the technical field of separation and purification of natural products, and discloses a process of a 1, 8-cineole enrichment method for refining eucalyptus oil through short-path molecular distillation. The method is characterized by comprising the following steps: carrying out selective hydroxylation or epoxidation reaction on crude eucalyptus oil and a buffer system containing F87A / A328G mutant CYP102A1 enzyme under temperature and oxygen control conditions to convert impurities into high-boiling-point derivatives, and collecting light components through short-path molecular distillation. By adopting the technical scheme, an enzymatic selective conversion-short-path molecular distillation coupling process is constructed, the key separation problem in the preparation of the high-purity 1, 8-cineole is successfully solved, an efficient, green and large-scale technical path is provided, and the method is suitable for industrial production. The urgent requirements on high-purity natural monoterpene compounds in the fields of medicines, high-end daily chemicals and the like are met.

This invention belongs to the field of oil-in-water emulsion preparation technology, and relates to a pH-controlled gelatin-lecithin composite system stabilized oil-in-water emulsion and its preparation method. In the preparation process, lecithin, gelatin, and water are mixed to obtain solution A. The pH value of solution A is adjusted to 3-6. Finally, the pH-adjusted solution A is used as the aqueous phase, and then the oil phase is added to the aqueous phase. Emulsification is performed at a shear rate of 15000-18000 rpm to obtain the pH-controlled gelatin-lecithin composite system stabilized oil-in-water emulsion. The oil phase consists of cyclic monoterpenes. The particle size of the pH-controlled gelatin-lecithin composite system stabilized oil-in-water emulsion is 1-15 μm. The method of this invention is simple and easy to operate; the obtained pH-controlled gelatin-lecithin composite system stabilized oil-in-water emulsion exhibits good dispersibility and stability.

The application discloses application of proteins and related biological materials in the field of variation or genetic engineering in promoting monoterpenes accumulation in grape fruits. The technical problem to be solved by the application is how to improve monoterpenes content in grape fruits. One technical solution to be protected by the application is application of a protein with an amino acid sequence of sequence 2 in the sequence listing in improving monoterpenes content in grape fruits. Experiments prove that VvHYH derived from grape can promote monoterpenes accumulation in grape fruits and leaves, and the application can be applied to grape flavor improvement and breeding.

The invention discloses a tobacco terpenoid synthase gene NtTPS2184 and application thereof, and belongs to the technical field of plant molecular biology, the nucleotide sequence of the tobacco terpenoid synthase gene NtTPS2184 is shown as SEQ ID NO.1, the invention provides a recombinant expression vector, the recombinant expression vector is obtained by positively inserting the tobacco terpenoid synthase gene NtTPS2184 between BamHI and SacI sites of pGEX4.0 plasmid, and the recombinant expression vector is used for expressing the tobacco terpenoid synthase gene NtTPS2184. The invention also provides an application of the tobacco terpene synthase gene NtTPS2184 or the recombinant expression vector in the synthesis of linalool and trans-nerolidone by a catalytic substrate, and the terpene synthase gene NtTPS2184 provided by the invention can be massively expressed by using escherichia coli genetically engineered bacteria, so that the yield of the tobacco terpene synthase gene NtTPS2184 is increased, and the yield of the tobacco terpene synthase gene NtTPS2184 is increased. In the presence of a substrate geranyl pyrophosphate, the monoterpene synthase exerts the activity of the monoterpene synthase to catalytically synthesize linalool; and in the presence of a substrate farnesyl pyrophosphate, the activity of the sesquiterpene synthase is exerted, and the trans-nerolidinol is catalytically synthesized.

Polyamides are claimed that comprise at least 10% by weight of at least one cyclic terpenoid substructure. The substructure is preferably derived from a monoterpene. A method for preparing the polyamides and for producing the moulding compounds is also described. The moulding compounds comprise 10% to 90% by weight of these polyamides.

The invention discloses a monoterpene compound purification device, and relates to the technical field of monoterpene compound purification, the monoterpene compound purification device comprises an extraction tower and a plurality of filler frames fixedly connected to the inner side of the extraction tower; and the multiple elastic filler balls are arranged, the inner side of the filler frame is filled with the multiple elastic filler balls, and the inner side of each elastic filler ball is provided with multiple through holes penetrating through the interior. Through cooperation of the elastic filler balls and other parts, the mass transfer resistance between two-phase liquid in the purification process of the monoterpene compound can be reduced, and the transfer efficiency of the monoterpene component is remarkably improved; besides, when the elastic filler balls roll, the through holes can continuously change the flowing path of the liquid, so that a dead liquid area (a liquid flowing stagnation area) in a local area is avoided, all the liquid in the extraction tower can participate in the extraction reaction, and finally the extraction purity and the extraction yield of the monoterpene compound are improved.

The present invention relates to a method for preparing a halide perovskite film having a double-layer structure and excellent piezoelectric performance by using an eco-friendly solvent, a perovskite film prepared by using the same, and an energy harvesting device comprising the same. In the present invention, by using lactate ester and monoterpene, which are eco-friendly solvents, as solvents of the halide perovskite precursor, the perovskite layers can be stably formed, and a perovskite film having very excellent piezoelectric performance can be prepared according to the formation of a double-layer structure of a three-dimensional perovskite and a zero-dimensional perovskite. Therefore, by using the present invention, an environmental pollution problem caused by the use of a solvent during the formation of the perovskite film can be solved, and the piezoelectric performance of the film can be greatly improved through optimization of materials and structure.

The application discloses a gene of eudesmolsynthase AoTPS1 and an application of the gene and a coded protein, and provides a gene AoTPS1 for regulating biosynthesis of a monoterpenoid compound eudesmol, wherein a nucleotide sequence is shown as SEQ ID NO. 1, the total length of the sequence is 1785 bp, 594 amino acids are coded, the molecular weight is 69.03 kDa, and a coded amino acid sequence is shown as SEQ ID NO. 2. The eudesmolsynthase AoTPS1 protein has the function of catalyzing synthesis of eudesmol in vitro, meanwhile, overexpression of the eudesmolsynthase AoTPS1 gene by using a genetic transformation technology can significantly increase the content of eudesmol in monoterpenoid volatile oil compounds. Therefore, the AoTPS1 gene is a key gene for regulating synthesis of volatile oil of Eucommia ulmoides Oliv., and can significantly promote biosynthesis of eudesmol in monoterpenoid volatile oil and improve the medicinal value of volatile oil of Eucommia ulmoides Oliv.

The present invention provides a topical pharmaceutical composition that contains a nonsteroidal anti-inflammatory drug, a lower alcohol, vitamin E derivatives, and water, without the harmful effects caused by high concentrations of alcohol, and without suppressing the precipitation of insoluble substances. [Solution] The topical pharmaceutical composition of the present invention contains (A) a nonsteroidal anti-inflammatory drug, (B) a nonionic surfactant with an HLB value greater than 6.5, (C) 30-45% by weight of a lower alcohol, (D) an antihistamine, (E) 0.1-2% by weight of vitamin E derivatives, (F) 3-6% by weight of monoterpenes, and (G) water.